HPRP-A1, a 15-mer α-helical cationic peptide, was derived from N-terminus of ribosomal protein L1 (RpL1) of Helicobacter pylori. In this study, HPRP-A1 was used as a framework to obtain a series of peptide analogs wi...HPRP-A1, a 15-mer α-helical cationic peptide, was derived from N-terminus of ribosomal protein L1 (RpL1) of Helicobacter pylori. In this study, HPRP-A1 was used as a framework to obtain a series of peptide analogs with different hydrophobicity by single amino acid substitutions in the center of nonpolar face of the amphipathic helix in order to systematically study the effect of hydrophobicity on biological activities of -helical antimicrobial peptides. Hydrophobicity and net charge of peptides played key roles in the biological activities of these peptide analogs; HPRP-A1 and peptide analogs with relative higher hydrophobicity exerted broad spectrum antimicrobial activity against Gram-negative bacteria, Gram-positive bacteria and pathogenic fungi, but also showed stronger hemolytic activity; the change of hydrophobicity and net charge of peptides had similar effects with close trend and extent on antibacterial activities and antifungal activities. This indicated that there were certain correlations between the antibacterial mode of action and the antifungal mode of action of these peptides in this study. The peptides exhibited antimicrobial specificity for bacteria and fungi, which provided potentials to develop new antimicrobial drugs for clinical practices.展开更多
Antibacterial nanoagents with broad-spectrum antibacterial activities have emerged as an evolution of antibiotics.However,their bacteria-capturing capability and bacteria-killing efficiency remain insufficient for com...Antibacterial nanoagents with broad-spectrum antibacterial activities have emerged as an evolution of antibiotics.However,their bacteria-capturing capability and bacteria-killing efficiency remain insufficient for commercial implementation.Inspired by the hunting behavior of spiders,here,a novel spiderweb-like nanoagent based on silver-adenine nanowires is designed to achieve high-efficiency capture and killing of bacteria.By virtue of the initiative bacteriacapturing functionality,this spiderweb-like nanoagent could effectively attract and trap bacteria through electrostatic interaction and its reticular morphology feature.Furthermore,its synergetic antibacterial mechanism combining physical membranolysis with reactive oxygen species(ROS)release allows such nanoagent to harvest a promoted bactericidal activity.Importantly,the woundplast employing the spiderweblike nanoagent exhibits a superior antibacterial efficacy against drug-resistant Gram-negative Escherichia coli and Gram-positive methicillin-resistant Staphylococcus aureus compared with commercially available woundplasts.This work may pave a new way for rational design of new generation bactericidal agents facing the future super-bacteria booming.展开更多
基金Natural Science Foundation of Jilin Province(201015130)the Youth Foundation of Jilin Province (20100126)
文摘HPRP-A1, a 15-mer α-helical cationic peptide, was derived from N-terminus of ribosomal protein L1 (RpL1) of Helicobacter pylori. In this study, HPRP-A1 was used as a framework to obtain a series of peptide analogs with different hydrophobicity by single amino acid substitutions in the center of nonpolar face of the amphipathic helix in order to systematically study the effect of hydrophobicity on biological activities of -helical antimicrobial peptides. Hydrophobicity and net charge of peptides played key roles in the biological activities of these peptide analogs; HPRP-A1 and peptide analogs with relative higher hydrophobicity exerted broad spectrum antimicrobial activity against Gram-negative bacteria, Gram-positive bacteria and pathogenic fungi, but also showed stronger hemolytic activity; the change of hydrophobicity and net charge of peptides had similar effects with close trend and extent on antibacterial activities and antifungal activities. This indicated that there were certain correlations between the antibacterial mode of action and the antifungal mode of action of these peptides in this study. The peptides exhibited antimicrobial specificity for bacteria and fungi, which provided potentials to develop new antimicrobial drugs for clinical practices.
基金supported by the National Natural Science Foundation of China(21703230,21635007 and 21721003)K.C.Wong Education Foundation。
文摘Antibacterial nanoagents with broad-spectrum antibacterial activities have emerged as an evolution of antibiotics.However,their bacteria-capturing capability and bacteria-killing efficiency remain insufficient for commercial implementation.Inspired by the hunting behavior of spiders,here,a novel spiderweb-like nanoagent based on silver-adenine nanowires is designed to achieve high-efficiency capture and killing of bacteria.By virtue of the initiative bacteriacapturing functionality,this spiderweb-like nanoagent could effectively attract and trap bacteria through electrostatic interaction and its reticular morphology feature.Furthermore,its synergetic antibacterial mechanism combining physical membranolysis with reactive oxygen species(ROS)release allows such nanoagent to harvest a promoted bactericidal activity.Importantly,the woundplast employing the spiderweblike nanoagent exhibits a superior antibacterial efficacy against drug-resistant Gram-negative Escherichia coli and Gram-positive methicillin-resistant Staphylococcus aureus compared with commercially available woundplasts.This work may pave a new way for rational design of new generation bactericidal agents facing the future super-bacteria booming.
