环境细菌遗传多样性分析的基因标签串测序法

把研究真核细胞表达基因种类和水平的基因标签串测序法引入环境细菌遗传多样性分析 ,选择细菌 1 6S核糖体RNA基因 (rDNA)内一 2 6碱基对高变异区段为该基因变异类型的标签 ,连接成标签串并测序 ,用标签类型、频率和遗传多样性指数等参数在分子水平上分析环境细菌遗传多样性 ,建立了环境细菌遗传多样性分析的基因标签串测序法 .该方法在分析环境隐含遗传多样性、揭示污染生物学效应和评价环境质量等方面有广泛的应用价值 .因使用共同基因标签的数据容易相互比较和整合 ,该方法还特别有利于开展广地域、宽时限的合作调查 .

短波SFH/MFSK系统PN码串序捕获性能的分析

在ＪｏｖａｎｏｖｉｃＶＭ．所介绍的直接分析方法的基础上，对单、双驻留方式的串序捕获性能作了分析和计算．结果表明，双驻留方式优于单驻留方式．最后，通过数值模拟，确定了适用于短波ＳＦＨ／ＭＦＳＫ系统的ＰＮ码周期，并估算了该系统的平均捕获时间．

四元同名元素不相邻可重复排列数的算法

研究四元同名元素不相邻可重复排列数F(a,b,c,d)的一般性质,获得了排列数F(a,a,a,a)和F(a,a,c,d)的递推公式以及F(1,1,c,d)的计算公式。

六角格网的弧线矢量数据量化拟合方法

兵棋地图矢量化是指将地理信息因子以属性形式映射到以六角网格为组织结构的兵棋地图中的过程.由于地理信息连通结构复杂,其中包含大量的有向弧段数据,如何在保持原有拓扑连接的前提下快速准确地量化到六角网格一直是一个困难的问题.对此,提出一种串序匹配拟合方法.首先依据有向弧段生成所穿越的匹配单元序列;然后对每个匹配单元依据离散Fréchet距离确定最佳的拟合六角格边;最后寻找并连接各条有向格边序列的连通端点,形成连通图.实验结果表明,文中方法可以在线性时间内完成大量弧段的量化拟合计算;同时,由于在量化中弧段间的连接节点被调整到格点,各弧段间的连接关系可在拟合过程中得以保持;串序匹配拟合方法具有很好的适应性和很高的效率.

An improved linear dispersion codes transmission scheme based on OSIC detection

Linear dispersion codes (LDCs) were originally designed based on maximum likelihood detection. They do not have good performance when using ordered successive interference cancellation (OSIC) detection. In this paper,we propose a new improved linear dispersion codes transmission scheme to combat performance loss of original LDCs when using OSIC detection. We introduce an interleaver to each data substream transmitted over different antennas after LDCs encoder. Furthermore,a new computer search criterion for a linear transformation matrix is also proposed. New search criterion is to minimize the symbol error rate based on OSIC detection. Computer simulations show that the performance of proposed LDCs transmission scheme is better than the original LDCs.

Structure of Mitochondrial DNA Control Region of Pholis fangi and Its Phylogenetic Implication

In this study, the entire mitochondrial DNA(mtDNA) control region(CR) of Pholis fangi was amplified via polymerase chain reaction followed by direct sequencing. The length of the mtDNA CR consensus sequence of P. fangi was 853 bp in length. In accordance with the recognition sites as were previously reported in fish species, the mtDNA CR sequence of P. fangi can be divided into 3 domains, i.e., the extended terminal associated sequence(ETAS), the central conserved sequence block(CSB), and the CSB domain. In addition, the following structures were identified in the mtDNA CR sequence of P. fangi: 2 ETASs in the ETAS domain(TAS and cTAS), 6 CSBs in the central CSB domain(CSB-F to CSB-A), and 3 CSBs in the CSB domain(CSB-1 to CSB-3). These demonstrated that the structure of the mtDNA CR of P. fangi was substantially different from those of most other fish species. The mtDNA CR sequence of P. fangi contained one conserved region from 656 bp to 815 bp. Similar to most other fish species, P. fangi has no tandem repeat sequences in its mtDNA CR sequence. Phylogenetic analysis based on the complete mtDNA CR sequences showed that there were no genetic differences within P. fangi populations of the same geographical origin and between P. fangi populations of different geographical origins.

Phylogeography of Batrachospermum arcuatum in North China based on ITS sequence data

Batrachospermum arcuatum Kylin is typically dioecious,although some monoecious specimens have been collected from four locations in North China.In this study,B.arcuatum populations,including monoecious and dioecious thalli,were collected from seven stream segments.The nuclear DNA internal transcribed spacer(ITS)region was sequenced for the seven populations,and a phylogenetic tree was constructed using Bayesian inference to assess intraspecifi c relationships.A haplotype network was also created.The ITS region in B.arcuatum from North China comprised 822–853 bp,with 10 haplotypes detected from the seven locations.The results of this study support the inclusion of monoecious individuals in the species B.arcuatum.

Spatiotemporal evolution of high-aspect-ratio filamentary trace in sapphire of picosecond pulse burst-mode for laser lift-off

The influence of the picosecond(ps) pulsed burst with a nanosecond scale of temporal separation(50 ns) on filamentary traces in sapphire substrate is investigated. The spatiotemporal evolution of the filamentary plasma string induced by sub-pulses of the burst-mode is revealed according to the analysis of the instantaneous photoluminescence images. Due to the presence of residual plasma, the energy loss of sub-pulse during the balancing of self-focusing effect is reduced, and thus refreshes the plasma via refocusing. The refreshed plasma peak generated by the subsequent subpulse appears at relatively low density positions in the formed filamentary plasma string, which results in more uniform densities and less spatial overlap among the plasma peaks. The continuity and uniformity of the filamentary trace in sapphire are enhanced by the burst-mode. Besides, the burst filamentary propagation can also remain effective when the sub-pulse energy is below the self-focusing threshold. Based on this uniform and precise energy propagation mode, the feasibility of its use for the laser lift-off(LLO) process is verified.

Identification and Analysis of SSRs Derived from Protein-coding Genes in Grape

SSR(Simple Sequence Repeats), also known as microsatellites or STRs(short tandem repeats), are a type of PCRbased markers. So far, the version of grape genome has been updated constantly, but SSRs derived from protein-coding genes in grape have not yet been identified. In this study, 4 337 SSR-containing genes were found among 29 971 protein-coding genes in grape(Vitis vinifera L.), and 5 384 SSRs were found. There were 96 types of repeat motifs in SSRs derived from protein-coding genes in grape, and the most frequently occurring repeat motif was A/T. Among various repeat motifs in dinucleotide SSRs, the most frequently occurring repeat motif was AG/CT. Moreover, many genes exhibited codon usage bias, which was affected by the mutation pressure. GO annotation, KEGG annotation and domain analysis of these genes were performed.Several genes were found to be closely related to the synthesis and metabolism of secondary metabolites, synthesis of flavones or anthocyanins, development and morphology of plant organs, and tolerance to biotic or abiotic stresses, including transcription factors in MYB, Hsf, NBS and TPC families. This study laid a solid foundation for the development of SSR markers and research of QTLs controlling complex agronomic traits in grape.

Genotyping of White Spot Syndrome Virus in Chinese Cultured Shrimp during 1998-1999

Recent studies showed that white spot syndrome virus(WSSV) isolates from different geographic locations share a high genetic similarity except the variable regions in ORF23/24 and ORF14/15,and variable number of tandem repeats(VNTR) within ORF94.In this study,genotyping was performed according to these three variable regions among WSSV isolates collected during 1998/1999 from Southern China.These WSSV isolates contain a deletion of 1168,5657,5898,9316 and 11093 bp,respectively in the variable region ORF23/24 compared with WSSV-TW,and a deletion of 4749 or 5622 bp in the variable region ORF14/15 relative to TH-96-II.Four types of repeat units(RUs)(6,8,9 and 13 RUs) in ORF94 were detected in these isolates,with the shortest 6 RUs as the most prevalent type.Our results provide important information for a better understanding of the spatio-temporal transmission mode and the WSSV genetic evolution lineage.

Simplified microsatellite instability detection protocol provides equivalent sensitivity to robust detection strategies in Lynch syndrome patients

Objective:Germline mutations in mismatch repair(MMR)genes cause Lynch syndrome(LS).LS is an inherited disease,and an important consequence of MMR deficiency is microsatellite instability(MSI)phenotype.MSI phenotype influences the efficacy of5 fluorouracil(5-FU)chemotherapy.Reproducible,cost effective,and easy to perform laboratory tests are required to include MSI detection in routine laboratory practice.Evaluation of CAT25 as monomorphic short tandem repeat sequence enables CAT25 to be an efficient screening tool among hereditary nonpolyposis colorectal cancer(HNPCC)patients compared with other methods used currently.Methods:Based on Amsterdam II criteria,31 patients in 31 families were shortlisted from a total number of 1,659 colorectal cancer patients.MSI status was examined in these patients using CAT25 and a commercially available Promega MSI five-markerbased detection system as well as immunohistochemical(IHC)staining of four important MMR proteins.Patients were scored as high microsatellite instable(MSI-H),low(MSI-L),or stable(MSS).MSI status determined by CAT25 single mononucleotide marker was compared with that of five mononucleotide markers,Promega commercial kit,and IHC method.Results:MMR protein deficiency was observed on 7/31 probands using IHC methodology and 6/31 categorized as MSI-H using commercial kit or CAT25 single marker.The sensitivity and specificity of the CAT25 single marker were the same as those detected by five-marker Promega commercial kit in our patients.Conclusions:Based on our results,the performance of the CAT25 single mononucleotide marker for MSI status determination in our HNPCC patients is the same as that of the five-marker-based commercial kit.

Broader pattern of tandem repeats in the mitochondrial control region of Perciformes

Perciformes,the largest order of vertebrates with 20 suborders,is the most diverse fish order that dominates vertebrate ocean life.The complete mitochondrial control region(CR) of Trichiurus japonicus(Trichiuridae,Scombroidei) and Pampus sp.(Stromateidae,Stromateoidei) were amplified and sequenced.Together with data from GenBank,the tandem repeats in the mitochondrial CR from 48 species,which covered nine suborders of Perciformes,are reported in this study.The tandem repeats tend to be long in the suborder Percoidei and Stromateoidei.The identical repeats in 21 species of Cichlidae suggest a common origin and have existed before species divergence.Larimichthys crocea shows tandem repeats instead of the typical structure of the central conserved sequence blocks,which was first reported in Perciformes and vertebrates.This might have resulted from interruption of the polymerase activity during the H-strand synthesis.The four broader patterns presented here for the tandem repeats,including those in both the 5' and 3' ends,only in the either 5' or 3' end,and in the central conserved domain of the control region,will be useful for understanding the evolution of species.

Genetically identified complete hydatidiform mole coexisting with a normal fetus following IVF-ET

A complete hydatidiform mole coexisting with a fetus following in vitro fertilization and embryo transfer (IVF-ET) is a rare event. The diagnosis is often not easy because of the morphological similarity to a partial mole, but important to the treatment. We present a recent case in which STR polymorphism analysis clearly revealed a different genetic origin for the fetal and molar parts. STR polymorphisms on 15 variable number tandem repeat loci and a gender-determination locus, which were detected by polymerase chain reaction, indicating that the cord/placenta and molar tissue were parental and androgenous, respectively. During follow-up, the patient developed persistent gestational trophoblastic tumor (GTT) which was successfully treated with chemotherapy. In this case, STR polymorphism analysis exactly diagnosed a twin pregnancy consisting of a complete hydatidiform mole and a fetus.

A Single Pattern Matching Algorithm Based on Character Frequency

Based on the study of single pattern matching, MBF algorithm is proposed by imitating the string searching procedure of human. The algorithm preprocesses the pattern by using the idea of Quick Search algorithm and the already-matched pattern psefix and suffix information. In searching phase, the algorithm makes use of the!character using frequency and the continue-skip idea. The experiment shows that MBF algorithm is more efficient than other algorithms.

The Application of the Fault Current Limiters in Power Distribution System

In this paper, an application of fault current limiter-thyristor controller series reactor （FCL-TCSR） in the distribution network is presented in order to minimize the peak value of current during the fault condition. This application considers a single fault current limiting action but can also be applied for a three phase system. The maximum contribution of FCL-TCSR quickly clears the abnormal current in the power distribution system when a fault condition occurs. Using a mathematical model of FCL-TCSR, the impact of series impedance used to adjust the amplitude of the fault current action is demonstrated. The performance of the load with the impedance and load in series is also analyzed.

A New Multi-platform Data Acquisition System for Power Quality Metrological Certification

In order to provide power quality monitoring activities with metrological certification, a DAS （data acquisition system） has been designed, realized and characterized. The system allows acquisition on tri-phases plus neutral lines picking up 499 samples per period of the fundamental at 50 Hz. To ensure certified measurements, the system gets external certified time and voltage references. The system uses a FTDI Virtual Com Port Driver to communicate data over High Speed RS232 virtual interface and it does not need any advanced programming skill. The choice to use a virtual serial communication makes the data acquisition software portable over many platforms, regardless by the development environment and by the programming language. To test the proposed device some custom software have been written in many programming language （C^#, VB6, LabView, MatLab）, moreover in order to characterize the device the most common ADC （analog to digital converter） performing test have been applied.

一个特殊可重复排列数的算法

研究一类特殊的可重复排列性质,建立了一般情形下求这类排列数的递推公式,导出了一些特殊情形下的具体计算公式.

Two tandem multicomponent reactions for the synthesis of sequence-defined polymers

Multicomponent polymerizations have become powerful tools for the construction of sequence-defined polymers. Although the Passerini multicomponent reaction has been widely used in the synthesis of sequence-defined polymers, the tandem usage of the Passerini multicomponent reaction and other multicomponent reactions in one-pot for the synthesis of sequence-defined polymers has not been developed until now. In this contribution, we report the tandem usage of the Passerini three-component reaction and the three-component amine-thiol-ene conjugation reaction in one pot for the synthesis of sequence-defined polymers. The Passerini reaction between methacrylic acid, adipaldehyde, and 2-isocyanobutanoate was carried out, affording a new molecule containing two alkene units. Subsequently, an amine and a thiolactone were added to the reaction system, whereupon the three-component amine-thiol-ene conjugating reaction occurred to yield a sequence-defined polymer. This method offers more rapid access to sequence-defined polymers with high molecular diversity and complexity.

Rapid, cost-effective DNA quantification via a visuallydetectable aggregation of superparamagnetic silicamagnetite nanoparticles

DNA and silica-coated magnetic particles entangle and form visible aggregates under chaotropic conditions with a rotating magnetic field, in a manner that enables quantification of DNA by image analysis. As a means of exploring the mechanism of this DNA quantitation assay, nanoscale SiO2-coated Fe304 （Fe3O4@SiO2） particles are synthesized via a solvothermal method. Characterization of the particles defines them to be -200 nm in diameter with a large surface area （141.89 m2/g）, possessing superparamagnetic properties and exhibiting high saturation magnetization （38 emu/g）. The synthesized Fe3O4@SiO2 nanoparticles are exploited in the DNA quantification assay and, as predicted, the nanoparticles provide better sensitivity than commercial microscale Dynabeads for quantifying DNA, with a detection limit of 4 kilobase-pair fragments of human DNA. Their utility is proven using nanoparticle DNA quantification to guide efficient polymerase chain reaction （PCR） amplification of short tandem repeat loci for human identification.

Genetic polymorphism analyses of a novel panel of 19 X-STR loci in the Chinese Uygur ethnic minority

The population genetic data and forensic parameters of 19 X-chromosome short tandem repeat （X-STR） loci in Chinese Uygur ethnic minority are presented. These loci were detected in a sample of 233 （94 males and 139 females） unrelated healthy individuals. We observed 238 alleles at the 19 X-STR loci, with the corresponding gene frequencies spanning the range from 0.0021 to 0.5644. After Bonferroni correction （P〉0.0026）, there were no signif- icant deviations from Hardy-Weinberg equilibrium. The cumulative power of discrimination in females and males, and the probability of exclusion of the 19 X-STR loci were 0.999 999 999 999 999 999 998 091,0.999 999 999 999 966, and 0.999 999 986 35, respectively. The cumulative mean exclusion chance was 0.999 999 992 849 in deficiency cases, 0.999 999 999 999 628 in normal trios, and 0.999 999 998 722 in duo cases. The high value of the forensic parameters mentioned above revealed that the novel panel of 19 loci had important values for forensic applications in the LJygur group.