采用基于液相色谱-高分辨质谱的转录因子结合序列串联多拷贝双联DNA结合元件(Concatenated tandem array of transcription factor response elements,catTFRE)Pull down蛋白质组学技术,研究了曲妥珠单抗耐药胃癌细胞的转录因子及其调...采用基于液相色谱-高分辨质谱的转录因子结合序列串联多拷贝双联DNA结合元件(Concatenated tandem array of transcription factor response elements,catTFRE)Pull down蛋白质组学技术,研究了曲妥珠单抗耐药胃癌细胞的转录因子及其调控作用。以合成的串联转录因子结合序列DNA片段为亲和试剂,用生物素标记,作为"DNA诱饵",通过Pull down富集后,采用液相色谱-高分辨质谱检测捕获的转录因子,基于强度定量法(Intensity based absolute quantification,iBAQ)定量,筛选出与DNA结合活性显著变化的转录因子。利用WebGestalt(2017)数据库对激活转录因子调控的信号通路、家族分类、调控网络及转录因子-靶基因(TFs-targets)进行分析。结果表明,359个转录因子被定量检测,与亲本细胞相比,61个转录因子在曲妥珠单抗耐药胃癌细胞中与DNA结合活性显著变化,其中结合活性增强48个,活性降低13个。激活转录因子属于bZIP、bHLH、homebox、HMG box、Zine finger等家族。KEGG通路富集显示,癌症、MAPK、Wnt、TGF-β、凋亡等多条通路在耐药细胞中显著改变。TFs-targets分析表明,TCF7L2、TCF7、FOXC1、JUN、CYC、FOS、ELK4、NFκB2、DDIT3和ATF2在上皮-间质转化(Epithelial-mesenchymal transformation,EMT)、Wnt、MAPK信号通路促使胃癌曲妥珠单抗耐药过程中发挥重要调控作用,提示靶向这些转录因子所调控的信号通路可能是逆转胃癌曲妥珠单抗耐药的重要途径。展开更多
The subsidence of the mining area was monitored by analyzing the phase of permanent scatters （PS） which maintained high coherence in magnitude of SAR images.A hew method of spatial unwrapping was presented which use...The subsidence of the mining area was monitored by analyzing the phase of permanent scatters （PS） which maintained high coherence in magnitude of SAR images.A hew method of spatial unwrapping was presented which used the subsidence rates calculated on comer reflector （CR） points as constraints for PS network to perform the spatial unwrapping using the parametric adjustment method.The algorithm achieved the integration of CR data and PSInSAR algorithm.The colliery dense distributed area around Baisha reservoir was chosen as the study area in the experiment.The time series of subsidence from February in 2007 to February in 2010 is successfully inversed by using the periodic function to simulate the linear and nonlinear components of the deformation.The simulation results show that the accuracy can be ± 2.1 mm with the leveling data being used as the external validation data.展开更多
基金Project(2006AA12Z156)supported by the National High Technology Research and Development Program of ChinaProjects(40974006,40774003)supported by the National Natural Science Foundation of China+1 种基金Project supported by Construct Program of the Key Discipline in Hunan Province,ChinaProjects(2011GK3106,2011SK3077)supported by Science and Technology Department of Hunan Province,China
文摘The subsidence of the mining area was monitored by analyzing the phase of permanent scatters （PS） which maintained high coherence in magnitude of SAR images.A hew method of spatial unwrapping was presented which used the subsidence rates calculated on comer reflector （CR） points as constraints for PS network to perform the spatial unwrapping using the parametric adjustment method.The algorithm achieved the integration of CR data and PSInSAR algorithm.The colliery dense distributed area around Baisha reservoir was chosen as the study area in the experiment.The time series of subsidence from February in 2007 to February in 2010 is successfully inversed by using the periodic function to simulate the linear and nonlinear components of the deformation.The simulation results show that the accuracy can be ± 2.1 mm with the leveling data being used as the external validation data.
