The relation between narratives and agency can be sometimes considered as mutually constitutive. There are cases in which telling a story expresses higher degrees of agency, and respectively, agency is shaped as a nar...The relation between narratives and agency can be sometimes considered as mutually constitutive. There are cases in which telling a story expresses higher degrees of agency, and respectively, agency is shaped as a narrative that expresses the agent＇s reasons. From henceforth, I will contend that a narrative theory, beyond the personal identity problem, can also enlighten how the agent attains giving reasons for the action by making sense of sequences of events. In order to explain how agency is constituted from understanding and control, we must assume a primitive competence for narratives. Agency supposes an ordering that cannot be reduced to temporality, but expresses a certain sense of accomplishment that can be narratively constituted. In this context, I will examine Welleman＇s theory of emotions as an ordering framework of the events in structures of means-ends. A possible objection to his explanation of the agential narratives is that emotions themselves need to be understood as narrative processes. I suggest then that an enactivist approach would be a way of explaining the narrative constitution of agency. One virtue of this approach is that it harmonizes the biological and cultural components of the agency from the most basic layers to the ordinary folk-psychological narratives.展开更多
β-carotene ketolase and β-carotene hydroxylase encoded by bkt and bch, respectively, are key enzymes required for astaxanthin biosynthesis in Haematococcu pluvialis 34-1n. Two expression vectors containing cDNA sequ...β-carotene ketolase and β-carotene hydroxylase encoded by bkt and bch, respectively, are key enzymes required for astaxanthin biosynthesis in Haematococcu pluvialis 34-1n. Two expression vectors containing cDNA sequences of bkt and bch were constructed and co-transformed into cell-wall-deficient Chlamydomonas reinhardtii CC-849. Transgenic algae were screened on TAP agar plates containing 10 gg mL 1 Zeomycin. PCR-Southern analysis showed that bkt and bch were integrated into the genomes of C. reinhardtii. Transcripts of bkt and bch were further confirmed by RT-PCR-Southern analysis. Compared with the wild type, transgenic algae produced 29.04% and 30.27% more carotenoids and xanthophylls, respectively. Moreover, the transgenic algae could accumulate 34% more astaxanthin than wild type. These results indicate that foreign bkt and bch genes were successfully translated into β-carotene ketolase and β-carotene hydroxylase, which were responsible for catalyzing the biosynthesis of astaxanthin in transgenic algae.展开更多
文摘The relation between narratives and agency can be sometimes considered as mutually constitutive. There are cases in which telling a story expresses higher degrees of agency, and respectively, agency is shaped as a narrative that expresses the agent＇s reasons. From henceforth, I will contend that a narrative theory, beyond the personal identity problem, can also enlighten how the agent attains giving reasons for the action by making sense of sequences of events. In order to explain how agency is constituted from understanding and control, we must assume a primitive competence for narratives. Agency supposes an ordering that cannot be reduced to temporality, but expresses a certain sense of accomplishment that can be narratively constituted. In this context, I will examine Welleman＇s theory of emotions as an ordering framework of the events in structures of means-ends. A possible objection to his explanation of the agential narratives is that emotions themselves need to be understood as narrative processes. I suggest then that an enactivist approach would be a way of explaining the narrative constitution of agency. One virtue of this approach is that it harmonizes the biological and cultural components of the agency from the most basic layers to the ordinary folk-psychological narratives.
基金supported by the National Natural Science Foundation of China(41176106,31470389,31470431)Shenzhen Grant Plan for Science & Technology(CXB201104210005A,JCYJ20120613112512654,JSGG20130411160539208)Guangdong Enterprise Academician Workstation(2011A090700015)
文摘β-carotene ketolase and β-carotene hydroxylase encoded by bkt and bch, respectively, are key enzymes required for astaxanthin biosynthesis in Haematococcu pluvialis 34-1n. Two expression vectors containing cDNA sequences of bkt and bch were constructed and co-transformed into cell-wall-deficient Chlamydomonas reinhardtii CC-849. Transgenic algae were screened on TAP agar plates containing 10 gg mL 1 Zeomycin. PCR-Southern analysis showed that bkt and bch were integrated into the genomes of C. reinhardtii. Transcripts of bkt and bch were further confirmed by RT-PCR-Southern analysis. Compared with the wild type, transgenic algae produced 29.04% and 30.27% more carotenoids and xanthophylls, respectively. Moreover, the transgenic algae could accumulate 34% more astaxanthin than wild type. These results indicate that foreign bkt and bch genes were successfully translated into β-carotene ketolase and β-carotene hydroxylase, which were responsible for catalyzing the biosynthesis of astaxanthin in transgenic algae.
