Aiming at the problem of on-line damage diagnosis in structural health monitoring (SHM), an algorithm of feature extraction and damage alarming based on auto-regressive moving-average (ARMA) time series analysis i...Aiming at the problem of on-line damage diagnosis in structural health monitoring (SHM), an algorithm of feature extraction and damage alarming based on auto-regressive moving-average (ARMA) time series analysis is presented. The monitoring data were first modeled as ARMA models, while a principalcomponent matrix derived from the AR coefficients of these models was utilized to establish the Mahalanobisdistance criterion functions. Then, a new damage-sensitive feature index DDSF is proposed. A hypothesis test involving the t-test method is further applied to obtain a decision of damage alarming as the mean value of DDSF had significantly changed after damage. The numerical results of a three-span-girder model shows that the defined index is sensitive to subtle structural damage, and the proposed algorithm can be applied to the on-line damage alarming in SHM.展开更多
Long-PCR amplification, clone and primer-walking sequencing methods were employed in determine the complete sequence of mitochondrial genome of tokay (Gekko gecko). The genome is 16 435 bp in size, contains 13 protein...Long-PCR amplification, clone and primer-walking sequencing methods were employed in determine the complete sequence of mitochondrial genome of tokay (Gekko gecko). The genome is 16 435 bp in size, contains 13 protein-coding, 2 ribosomal and 22 transfer RNA genes. The mt genome of Gekko is similar to most of the vertebrates in gene components, order, orientation, tRNA structures, low percentage of guanine and high percentage of thymine, and skews of base GC and AT. Base A was preferred at third codon positions for protein genes is similar to amphibians and fishes rather than amnion vertebrates. The standard stop codes (TAA) present only in three protein genes, less than those of most vertebrates. Transfer RNA genes range in length from 63 to 76 nt, their planar structure present characteristic clover leaf, except for tRNA-Cys and tRNA-Ser (AGY) because of lacking the D arm.展开更多
Let X be a metric space with an ordering structure,A: X→X is a operator and x≤Ax for any x∈X. In this paper we prove a new fixed point theorem, which generalizes famous caristi fixed point theorem.
Zeocin can cause double strand breaks of DNA and thus may be employed as a mutagen. In this study, two strains of Nannochloropsis oceanica, the wild and the Zeocin-tolerant strains, were re-sequenced to verify such fu...Zeocin can cause double strand breaks of DNA and thus may be employed as a mutagen. In this study, two strains of Nannochloropsis oceanica, the wild and the Zeocin-tolerant strains, were re-sequenced to verify such function of Zeocin, The results showed that Zeocin can mutate the N. oceanica genome and cause the structural variation. Zeocin either swept away or selected the alleles of genes functioning in ubiquitin-mediated proteolysis, alpha-linolenic acid metabolism, ascorbate and aldarate metabolism, ribosome biogenesis, and circadian rhythm, indicating that N. oceanica may have adjusted its metabolic performances for protein, carbohydrate, and lipid, and changed its ribosome biosynthesis and living rhythm to survive in Zeocin containing medium. In addition, Zeocin caused mutation may have influenced the expression of a set of tanscription factors. It was concluded that Zeocin effectively caused the structural variation of the genome of N. oceanica, and forced the microalgae to select out the alleles of a set of genes around these variations in order to adapt to Zeocin containing medium. Further studies on the genetic basis of the phenotypic adaptation of this haploid and asexual microalga and the application of Zeocin to its genetic improvement are very important.展开更多
This paper presents some insights on the state-of-the-art practice that has been utilized recently in the inplace structural strength and fatigue analysis for topsides on deepwater floating platforms such as tension l...This paper presents some insights on the state-of-the-art practice that has been utilized recently in the inplace structural strength and fatigue analysis for topsides on deepwater floating platforms such as tension leg plat-form (TLP) and semi-submersibles. Emphases are put on analysis software,geometric and mass modeling,hydro-dynamic loading and its mapping,and analysis procedures. In addition,for the in-place analysis using structure analysis computer system (SACS),the procedure of Visual Basic for Application (VBA) is developed to map AQWA-LINE hydrodynamic loading to the SACS integrated hull/topsides model;for the in-place analysis using structure engineering system analysis model (SESAM),many computer aided applications are made to aid the post-processing. These applications have been used in structural analyses for a few TLP and semi-submersible plat-form topsides,and are briefly introduced in this paper.展开更多
In this paper, in order to predict the residual deformation of thick spherical structure, a welding program is compiled in APDL language based on Ansys and a numerical welding experiment of a welding example is carrie...In this paper, in order to predict the residual deformation of thick spherical structure, a welding program is compiled in APDL language based on Ansys and a numerical welding experiment of a welding example is carried out. The temperature field of welding was simulated firstly, then a thermal-structure coupling analysis was carried out, and at last the residual stress and deformation after welding were got. After that, the numerical experiment result was compared with physical experiment one. The comparative analysis shows that the numerical simulation fits well with physical experiment. On the basis of that, a three-dimensional numerical experiment of a thick spherical shell structure was carried out to get the changing rule of stress and deformation of a thick spherical shell structure during welding. The research is of great value to the prediction of residual deformation and high precision machining.展开更多
In this paper, an FEM (Finite Element Method) model is established for the main span of the bridge, with the main box arch and suspender members modeled by beam elements, truss members by truss elements, and the ort...In this paper, an FEM (Finite Element Method) model is established for the main span of the bridge, with the main box arch and suspender members modeled by beam elements, truss members by truss elements, and the orthotropic steel deck by plate elements. The natural frequencies and mode shapes are acquired by the eigen-parameter analysis. By input of a typical earthquake excitation to the bridge system, the dynamic responses of the bridge, including the displacement and accelerations of the main joints of the structure, and the seismic forces and stresses of the key members, are calculated by the structural analysis program, based on which the main laws of the seismic responses of the bridge are summarized, and the safety of the structure is evaluated.展开更多
This paper deals with new and innovative case studies of application of post-tensioning for restoration of structures for buildings, bridges and rock stabilization in India. In earlier situations for these types of ca...This paper deals with new and innovative case studies of application of post-tensioning for restoration of structures for buildings, bridges and rock stabilization in India. In earlier situations for these types of cases conventional method of repair and rehabilitation has been used. These innovative approaches for restoration have led to enormous saving of cost and time. The advantages of post tensioning are well known in the civil industry and are being used for the last 40 to 50 years. Post tensioning is one of the best methods to induce stresses in the concrete before application of live load and this technique can also be widely used for restoration and rehabilitation of structures. Unlike the use of post-tensioning in new structures there are no definite methods or codes for application in restoration and rehabilitation of structures. For restoration and rehabilitation of structures, PT (post tensioned) technique can be applied in combination with other techniques and materials available. Bridge lifting is a tricky and risky job especially when the superstructure is displaced appreciably by a natural calamity, etc.. Not only the present state of structure needs proper study before commencement of restoration and rehabilitation but also calls for careful execution. This paper deals with case studies of innovative applications of post tensioning in restoration and rehabilitation of structures and restoration of bridge structure in Andaman & Nicobar Island, India affected by Sumatra quake.展开更多
Based on parameter design language, a program of progressive failure analysis in composite structures is proposed. In this program, the relationship between macro- and micro-mechanics is established and the macro stre...Based on parameter design language, a program of progressive failure analysis in composite structures is proposed. In this program, the relationship between macro- and micro-mechanics is established and the macro stress distribution of the composite structure is calculated by commercial finite element software. According to the macro-stress, the damaged point is found and the micro-stress distribution of representative volume element is calculated by finite-volume direct averaging micromechanics(FVDAM). Compared with the results calculated by failure criterion based on macro-stress field(the maximum stress criteria and Hashin criteria) and micro-stress field(Huang model), it is proven that the failure analysis based on macro- and micro-mechanics model is feasible and efficient.展开更多
RNA-Seq, a high-throughput (HT) sequencing technique, has been used effectively in large-scale transcriptomic studies, and is particularly useful for improving gene structure information and mining of new genes. In ...RNA-Seq, a high-throughput (HT) sequencing technique, has been used effectively in large-scale transcriptomic studies, and is particularly useful for improving gene structure information and mining of new genes. In this study, RNA-Seq HT technology was employed to analyze the transcriptome of yak ovary. After lllurrlina-Solexa deep sequencing, 26826516 clean reads with a total of 4828772880 bp were obtained from the ovary library. Alignment analysis showed that 16992 yak genes mapped to the yak genome and 3734 of these genes were involved in alternative splicing. Gene structure refinement analysis showed that 7340 genes that were annotated in the yak genome could be extended at the 5' or 3' ends based on the alignments been the transcripts and the genome sequence. Novel transcript prediction analysis identified 6321 new transcripts with lengths ranging from 180 to 14884 bp, and 2267 of them were predicted to code proteins. BLAST analysis of the new transcripts showed that 1200-4933 mapped to the non-redundant (nr), nucleotide (nt) and/or SwissProt sequence databases. Comparative statistical analysis of the new mapped transcripts showed that the majority of them were similar to genes in Bos taurus (41.4%), Bos grunniens mutus (33.0%), Ovis aries (6.3%), Homo sapiens (2.8%), Mus musculus (1.6%) and other species. Functional analy- sis showed that these expressed genes were involved in various Gene Ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes pathways. GO analysis of the new transcripts found that the largest proportion of them was associated with reproduction. The results of this study will provide a basis for describing the normal transcriptome map of yak ovary and for future studies on yak breeding performance. Moreover, the results confirmed that RNA-Seq HT technology is highly ad- vantageous in improving gene structure information and mining of new genes, as well as in providing valuable data to expand the yak genome information.展开更多
Pushover analysis is common because of its conceptual simplicity and computational attractiveness in computing seismic demand.Considering that traditional pushover analysis is restricted in underground structures due ...Pushover analysis is common because of its conceptual simplicity and computational attractiveness in computing seismic demand.Considering that traditional pushover analysis is restricted in underground structures due to the stark differences in the seismic response characteristics of surface structures,this paper proposes a pushover analysis method for underground structures and its application in seismic damage assessment.First,three types of force distribution are presented based on ground response analysis.Next,the target displacements and analysis models are established according to force-based and performance-based design.Then,the pushover analysis procedure for underground structures is described.Next,the applicability of pushover analysis to underground structures is verified by comparing the responses of a Chongwenmen subway station determined by the proposed procedure and by nonlinear response history analysis.In addition,two other points are made:that the inverted triangular distribution of effective earthquake acceleration is more practical than the other two distributions,and that performance-based design is more effective than force-based design.Finally,a cyclic reversal loading pattern based on one cycle of reversal loads as an earthquake event is presented and applied to the seismic damage assessment of underground structures.The results show that the proposed pushover analysis can be effectively applied to the seismic design and damage assessment of underground structures.展开更多
Nanopores for DNA sequencing have drawn much attention due to their potentials to achieve amplification-free, low-cost, and high-throughput analysis of nuclei acids. The material configuration and fabrication of the n...Nanopores for DNA sequencing have drawn much attention due to their potentials to achieve amplification-free, low-cost, and high-throughput analysis of nuclei acids. The material configuration and fabrication of the nanopore has become one important consideration in the nanopore based DNA sequencing research. Among various materials, the newly emerged graphene has brought more opportunities to the development of sequencing technology because of its unique structures and properties. This review mainly focuses on the experimental aspects of graphene nanopore research including the nanopore fabrication methods and processes. Meanwhile, the challenges in the present graphene nanopore research including hydrophobicity, translocation velocity and noise are also addressed and discussed.展开更多
This paper studies the damage-viscoelastic behavior of composite solid propellants of solid rocket motors(SRM).Based on viscoelastic theories and strain equivalent hypothesis in damage mechanics,a three-dimensional(3-...This paper studies the damage-viscoelastic behavior of composite solid propellants of solid rocket motors(SRM).Based on viscoelastic theories and strain equivalent hypothesis in damage mechanics,a three-dimensional(3-D)nonlinear viscoelastic constitutive model incorporating with damage is developed.The resulting viscoelastic constitutive equations are numerically discretized by integration algorithm,and a stress-updating method is presented by solving nonlinear equations according to the Newton-Raphson method.A material subroutine of stress-updating is made up and embedded into commercial code of Abaqus.The material subroutine is validated through typical examples.Our results indicate that the finite element results are in good agreement with the analytical ones and have high accuracy,and the suggested method and designed subroutine are efficient and can be further applied to damage-coupling structural analysis of practical SRM grain.展开更多
基金The National High Technology Research and Devel-opment Program of China (863Program) (No2006AA04Z416)the National Natural Science Foundation of China (No50538020)
文摘Aiming at the problem of on-line damage diagnosis in structural health monitoring (SHM), an algorithm of feature extraction and damage alarming based on auto-regressive moving-average (ARMA) time series analysis is presented. The monitoring data were first modeled as ARMA models, while a principalcomponent matrix derived from the AR coefficients of these models was utilized to establish the Mahalanobisdistance criterion functions. Then, a new damage-sensitive feature index DDSF is proposed. A hypothesis test involving the t-test method is further applied to obtain a decision of damage alarming as the mean value of DDSF had significantly changed after damage. The numerical results of a three-span-girder model shows that the defined index is sensitive to subtle structural damage, and the proposed algorithm can be applied to the on-line damage alarming in SHM.
文摘Long-PCR amplification, clone and primer-walking sequencing methods were employed in determine the complete sequence of mitochondrial genome of tokay (Gekko gecko). The genome is 16 435 bp in size, contains 13 protein-coding, 2 ribosomal and 22 transfer RNA genes. The mt genome of Gekko is similar to most of the vertebrates in gene components, order, orientation, tRNA structures, low percentage of guanine and high percentage of thymine, and skews of base GC and AT. Base A was preferred at third codon positions for protein genes is similar to amphibians and fishes rather than amnion vertebrates. The standard stop codes (TAA) present only in three protein genes, less than those of most vertebrates. Transfer RNA genes range in length from 63 to 76 nt, their planar structure present characteristic clover leaf, except for tRNA-Cys and tRNA-Ser (AGY) because of lacking the D arm.
文摘Let X be a metric space with an ordering structure,A: X→X is a operator and x≤Ax for any x∈X. In this paper we prove a new fixed point theorem, which generalizes famous caristi fixed point theorem.
基金funded by the National Natural Science Foundation of China(No.31270408)the National High Technology Research and Development Program(863 Program) of China(No.2014AA022001)
文摘Zeocin can cause double strand breaks of DNA and thus may be employed as a mutagen. In this study, two strains of Nannochloropsis oceanica, the wild and the Zeocin-tolerant strains, were re-sequenced to verify such function of Zeocin, The results showed that Zeocin can mutate the N. oceanica genome and cause the structural variation. Zeocin either swept away or selected the alleles of genes functioning in ubiquitin-mediated proteolysis, alpha-linolenic acid metabolism, ascorbate and aldarate metabolism, ribosome biogenesis, and circadian rhythm, indicating that N. oceanica may have adjusted its metabolic performances for protein, carbohydrate, and lipid, and changed its ribosome biosynthesis and living rhythm to survive in Zeocin containing medium. In addition, Zeocin caused mutation may have influenced the expression of a set of tanscription factors. It was concluded that Zeocin effectively caused the structural variation of the genome of N. oceanica, and forced the microalgae to select out the alleles of a set of genes around these variations in order to adapt to Zeocin containing medium. Further studies on the genetic basis of the phenotypic adaptation of this haploid and asexual microalga and the application of Zeocin to its genetic improvement are very important.
文摘This paper presents some insights on the state-of-the-art practice that has been utilized recently in the inplace structural strength and fatigue analysis for topsides on deepwater floating platforms such as tension leg plat-form (TLP) and semi-submersibles. Emphases are put on analysis software,geometric and mass modeling,hydro-dynamic loading and its mapping,and analysis procedures. In addition,for the in-place analysis using structure analysis computer system (SACS),the procedure of Visual Basic for Application (VBA) is developed to map AQWA-LINE hydrodynamic loading to the SACS integrated hull/topsides model;for the in-place analysis using structure engineering system analysis model (SESAM),many computer aided applications are made to aid the post-processing. These applications have been used in structural analyses for a few TLP and semi-submersible plat-form topsides,and are briefly introduced in this paper.
文摘In this paper, in order to predict the residual deformation of thick spherical structure, a welding program is compiled in APDL language based on Ansys and a numerical welding experiment of a welding example is carried out. The temperature field of welding was simulated firstly, then a thermal-structure coupling analysis was carried out, and at last the residual stress and deformation after welding were got. After that, the numerical experiment result was compared with physical experiment one. The comparative analysis shows that the numerical simulation fits well with physical experiment. On the basis of that, a three-dimensional numerical experiment of a thick spherical shell structure was carried out to get the changing rule of stress and deformation of a thick spherical shell structure during welding. The research is of great value to the prediction of residual deformation and high precision machining.
基金Acknowledgments: This study is sponsored by the Natural Science Foundation of China (No. 90715008) and the Flander (Belgium)-China Bilateral Project (No. BIL07/07).
文摘In this paper, an FEM (Finite Element Method) model is established for the main span of the bridge, with the main box arch and suspender members modeled by beam elements, truss members by truss elements, and the orthotropic steel deck by plate elements. The natural frequencies and mode shapes are acquired by the eigen-parameter analysis. By input of a typical earthquake excitation to the bridge system, the dynamic responses of the bridge, including the displacement and accelerations of the main joints of the structure, and the seismic forces and stresses of the key members, are calculated by the structural analysis program, based on which the main laws of the seismic responses of the bridge are summarized, and the safety of the structure is evaluated.
文摘This paper deals with new and innovative case studies of application of post-tensioning for restoration of structures for buildings, bridges and rock stabilization in India. In earlier situations for these types of cases conventional method of repair and rehabilitation has been used. These innovative approaches for restoration have led to enormous saving of cost and time. The advantages of post tensioning are well known in the civil industry and are being used for the last 40 to 50 years. Post tensioning is one of the best methods to induce stresses in the concrete before application of live load and this technique can also be widely used for restoration and rehabilitation of structures. Unlike the use of post-tensioning in new structures there are no definite methods or codes for application in restoration and rehabilitation of structures. For restoration and rehabilitation of structures, PT (post tensioned) technique can be applied in combination with other techniques and materials available. Bridge lifting is a tricky and risky job especially when the superstructure is displaced appreciably by a natural calamity, etc.. Not only the present state of structure needs proper study before commencement of restoration and rehabilitation but also calls for careful execution. This paper deals with case studies of innovative applications of post tensioning in restoration and rehabilitation of structures and restoration of bridge structure in Andaman & Nicobar Island, India affected by Sumatra quake.
基金Project(51075204)supported by the National Natural Science Foundation of ChinaProjects(2012ZB52026,2014ZB52024)supported by the Aeronautical Science Foundation of ChinaProject(NS2014024)supported by the Fundamental Research Funds for the Central Universities,China
文摘Based on parameter design language, a program of progressive failure analysis in composite structures is proposed. In this program, the relationship between macro- and micro-mechanics is established and the macro stress distribution of the composite structure is calculated by commercial finite element software. According to the macro-stress, the damaged point is found and the micro-stress distribution of representative volume element is calculated by finite-volume direct averaging micromechanics(FVDAM). Compared with the results calculated by failure criterion based on macro-stress field(the maximum stress criteria and Hashin criteria) and micro-stress field(Huang model), it is proven that the failure analysis based on macro- and micro-mechanics model is feasible and efficient.
基金supported by the National Science&Technology Pillar Program of China(2012BAD13B06)the Special Fund for Agroscientific Research in the Public Interest(201203009)
文摘RNA-Seq, a high-throughput (HT) sequencing technique, has been used effectively in large-scale transcriptomic studies, and is particularly useful for improving gene structure information and mining of new genes. In this study, RNA-Seq HT technology was employed to analyze the transcriptome of yak ovary. After lllurrlina-Solexa deep sequencing, 26826516 clean reads with a total of 4828772880 bp were obtained from the ovary library. Alignment analysis showed that 16992 yak genes mapped to the yak genome and 3734 of these genes were involved in alternative splicing. Gene structure refinement analysis showed that 7340 genes that were annotated in the yak genome could be extended at the 5' or 3' ends based on the alignments been the transcripts and the genome sequence. Novel transcript prediction analysis identified 6321 new transcripts with lengths ranging from 180 to 14884 bp, and 2267 of them were predicted to code proteins. BLAST analysis of the new transcripts showed that 1200-4933 mapped to the non-redundant (nr), nucleotide (nt) and/or SwissProt sequence databases. Comparative statistical analysis of the new mapped transcripts showed that the majority of them were similar to genes in Bos taurus (41.4%), Bos grunniens mutus (33.0%), Ovis aries (6.3%), Homo sapiens (2.8%), Mus musculus (1.6%) and other species. Functional analy- sis showed that these expressed genes were involved in various Gene Ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes pathways. GO analysis of the new transcripts found that the largest proportion of them was associated with reproduction. The results of this study will provide a basis for describing the normal transcriptome map of yak ovary and for future studies on yak breeding performance. Moreover, the results confirmed that RNA-Seq HT technology is highly ad- vantageous in improving gene structure information and mining of new genes, as well as in providing valuable data to expand the yak genome information.
基金supported by the Tsinghua Initiative Scientific Research Program(Grant No.2012THZ02-2)Beijing Natural Science Foundation(Grant No.8111001)+1 种基金National Basic Research Program of China(Grant No.2011CB013602)Major Research Plan of the National Natural Science Foundation of China(Grant No.91215301)
文摘Pushover analysis is common because of its conceptual simplicity and computational attractiveness in computing seismic demand.Considering that traditional pushover analysis is restricted in underground structures due to the stark differences in the seismic response characteristics of surface structures,this paper proposes a pushover analysis method for underground structures and its application in seismic damage assessment.First,three types of force distribution are presented based on ground response analysis.Next,the target displacements and analysis models are established according to force-based and performance-based design.Then,the pushover analysis procedure for underground structures is described.Next,the applicability of pushover analysis to underground structures is verified by comparing the responses of a Chongwenmen subway station determined by the proposed procedure and by nonlinear response history analysis.In addition,two other points are made:that the inverted triangular distribution of effective earthquake acceleration is more practical than the other two distributions,and that performance-based design is more effective than force-based design.Finally,a cyclic reversal loading pattern based on one cycle of reversal loads as an earthquake event is presented and applied to the seismic damage assessment of underground structures.The results show that the proposed pushover analysis can be effectively applied to the seismic design and damage assessment of underground structures.
基金supported by the National Natural Science Foundation of China (81471697)the Key Technology R&D Program of Hubei Province (2014BBB003)+1 种基金Yellow Crane Talent (Science & Technology) Program of Wuhan City and Applied Basic Research Program of Wuhan City (2016060101010044, 2016060101010048)the Fundamental Research Funds for the Central Universities (2016YXMS253)
文摘Nanopores for DNA sequencing have drawn much attention due to their potentials to achieve amplification-free, low-cost, and high-throughput analysis of nuclei acids. The material configuration and fabrication of the nanopore has become one important consideration in the nanopore based DNA sequencing research. Among various materials, the newly emerged graphene has brought more opportunities to the development of sequencing technology because of its unique structures and properties. This review mainly focuses on the experimental aspects of graphene nanopore research including the nanopore fabrication methods and processes. Meanwhile, the challenges in the present graphene nanopore research including hydrophobicity, translocation velocity and noise are also addressed and discussed.
基金supported by the National Natural Science Foundation of China(Grant No.11132012)
文摘This paper studies the damage-viscoelastic behavior of composite solid propellants of solid rocket motors(SRM).Based on viscoelastic theories and strain equivalent hypothesis in damage mechanics,a three-dimensional(3-D)nonlinear viscoelastic constitutive model incorporating with damage is developed.The resulting viscoelastic constitutive equations are numerically discretized by integration algorithm,and a stress-updating method is presented by solving nonlinear equations according to the Newton-Raphson method.A material subroutine of stress-updating is made up and embedded into commercial code of Abaqus.The material subroutine is validated through typical examples.Our results indicate that the finite element results are in good agreement with the analytical ones and have high accuracy,and the suggested method and designed subroutine are efficient and can be further applied to damage-coupling structural analysis of practical SRM grain.