[Objective] This study aimed to optimize the PCR amplification conditions for random ssDNA pool in SELEX technology. [Method] L16（45） orthogonal experimental design was adopted for optimization of five important fac...[Objective] This study aimed to optimize the PCR amplification conditions for random ssDNA pool in SELEX technology. [Method] L16（45） orthogonal experimental design was adopted for optimization of five important factors affecting PCR reaction system for random single-stranded DNA pool including Mg2＋ concentration, dNTP concentration, amount of Taq DNA polymerase, primer concentration and amount of random single-stranded DNA pool at four levels. Meanwhile, the annealing temperature and number of PCR reaction cycles were optimized to establish the optimal reaction system and PCR procedure. [Result] The optimal combination of PCR reaction system for random ssDNA pool was obtained, with a total system volume of 20 μl containing 2.0 μl of 10 × Buffer, 0.5 ng of random ssDNA pool, 2.5 mmol/L Mg2＋, 0.25 mmol/L dNTP Mixture, 0.6 μmol/L upstream and downstream primers and 1.5 U of Taq DNA polymerase; the optimal annealing temperature was 68 ℃ and the optimal number of cycles was 12. Under the above conditions, clear and stable bands with high specificity for random ssDNA pool were amplified. [Conclusion] This study laid the foundation for selection of parameters with higher specificity in SELEX technology.展开更多
On the basis of description of the necessity in construction of the Jiangxi red tourism resource E-C/C-E bilingual parallel corpus, this paper discusses the design and construction of the corpus. In its design, it des...On the basis of description of the necessity in construction of the Jiangxi red tourism resource E-C/C-E bilingual parallel corpus, this paper discusses the design and construction of the corpus. In its design, it describes the general design and the framework of the corpus, then it describes its construction including data collection, the standard for the sorted data, data selection, data digitalization, data tagging and data aligning. With the construction, it will not only realize purposes and functions of the corpus, but also provide others with ways or means to use the corpus and to establish such kind of corpus.展开更多
Using multimedia writing tools ‘Author ware＇ for Windows 3.0b18, and making a multimedia interface for a simple interface generated by FoxPro 2.5B, mainly to solve how to connect an interface with Author ware and Fo...Using multimedia writing tools ‘Author ware＇ for Windows 3.0b18, and making a multimedia interface for a simple interface generated by FoxPro 2.5B, mainly to solve how to connect an interface with Author ware and FoxPro database, namely the communication problem between two separate programs under the Windows environment and to generate the multimedia interface which is consistent with the status and requests for the management information system, namely the problem that how to establish multimedia interface design.展开更多
In order to improve the intelligence of the English teaching management, so as to improve the English teaching resource scheduling and sharing ability, a design method of teaching management system is proposed based o...In order to improve the intelligence of the English teaching management, so as to improve the English teaching resource scheduling and sharing ability, a design method of teaching management system is proposed based on Web. The system adopts Web network architecture design, mainly it is divided into MUC main control module, database module, data management module and man-machine interaction module, Network management and online scheduling of English teaching resources are obtained based on C/S framework, data exchange of English teaching resources is realized on client side and server side, the control component of the English teaching resource distribution database uses MBM29LV400BC as the logic decoding controller. The intelligent algorithm is used to analyze the big data to realize the scheduling and access control of the English teaching management system resources. The test results show that the design of the English teaching management system has good resource scheduling and database construction ability, and the overall intelligence of the system is better.展开更多
基金Supported by Central University Basic Research Operating Expenses Special Fund(XDJK2011C026)Southwest University Doctoral Fund(09BSR04)~~
文摘[Objective] This study aimed to optimize the PCR amplification conditions for random ssDNA pool in SELEX technology. [Method] L16（45） orthogonal experimental design was adopted for optimization of five important factors affecting PCR reaction system for random single-stranded DNA pool including Mg2＋ concentration, dNTP concentration, amount of Taq DNA polymerase, primer concentration and amount of random single-stranded DNA pool at four levels. Meanwhile, the annealing temperature and number of PCR reaction cycles were optimized to establish the optimal reaction system and PCR procedure. [Result] The optimal combination of PCR reaction system for random ssDNA pool was obtained, with a total system volume of 20 μl containing 2.0 μl of 10 × Buffer, 0.5 ng of random ssDNA pool, 2.5 mmol/L Mg2＋, 0.25 mmol/L dNTP Mixture, 0.6 μmol/L upstream and downstream primers and 1.5 U of Taq DNA polymerase; the optimal annealing temperature was 68 ℃ and the optimal number of cycles was 12. Under the above conditions, clear and stable bands with high specificity for random ssDNA pool were amplified. [Conclusion] This study laid the foundation for selection of parameters with higher specificity in SELEX technology.
文摘On the basis of description of the necessity in construction of the Jiangxi red tourism resource E-C/C-E bilingual parallel corpus, this paper discusses the design and construction of the corpus. In its design, it describes the general design and the framework of the corpus, then it describes its construction including data collection, the standard for the sorted data, data selection, data digitalization, data tagging and data aligning. With the construction, it will not only realize purposes and functions of the corpus, but also provide others with ways or means to use the corpus and to establish such kind of corpus.
文摘Using multimedia writing tools ‘Author ware＇ for Windows 3.0b18, and making a multimedia interface for a simple interface generated by FoxPro 2.5B, mainly to solve how to connect an interface with Author ware and FoxPro database, namely the communication problem between two separate programs under the Windows environment and to generate the multimedia interface which is consistent with the status and requests for the management information system, namely the problem that how to establish multimedia interface design.
文摘In order to improve the intelligence of the English teaching management, so as to improve the English teaching resource scheduling and sharing ability, a design method of teaching management system is proposed based on Web. The system adopts Web network architecture design, mainly it is divided into MUC main control module, database module, data management module and man-machine interaction module, Network management and online scheduling of English teaching resources are obtained based on C/S framework, data exchange of English teaching resources is realized on client side and server side, the control component of the English teaching resource distribution database uses MBM29LV400BC as the logic decoding controller. The intelligent algorithm is used to analyze the big data to realize the scheduling and access control of the English teaching management system resources. The test results show that the design of the English teaching management system has good resource scheduling and database construction ability, and the overall intelligence of the system is better.
