Background:During the production of tea,tea dust has been viewed as a risk factor that may cause the suffering of changes of lung function and respiratory symptoms.Objective:To review whether the occupational exposure...Background:During the production of tea,tea dust has been viewed as a risk factor that may cause the suffering of changes of lung function and respiratory symptoms.Objective:To review whether the occupational exposure to tea dust may lead to the changes of lung function and respiratory symptoms.Methods:We searched the literatures in March 2012 in the databases of Medline,EMBase,Web of Science,Wan-Fang Database and CNKI.The search condition is"(herb OR tea)AND(lung function OR ventilatory function OR pulmonary function OR spirometric measurement)".The paper should be pubished in Enghish or Chinese,with internal or external controls.Results:Totally 17 papers were included.Both acute and chronic changes of lung function were referred.In addition,the respiratory symptoms such as cough,tightness and etc.were also reported and discussed.Conclusion:We concluded that the exposure of tea dust may lead to the changes of lung function and respiratory symptoms.展开更多
Allergen specific IgE response is the major cause of immediate hypersensitivity. However the number of IgEproducing B cells and the amount of IgE, especially the specific IgE, are so low, it greatly impedes the study ...Allergen specific IgE response is the major cause of immediate hypersensitivity. However the number of IgEproducing B cells and the amount of IgE, especially the specific IgE, are so low, it greatly impedes the study of the allergic-specifc antibody responses. Here we report the construction of a normal human IgE combinatorial library The repertoire of IgE VH genes and of K genes were separately amplified from normal human peripheral blood lymphocytes through RT-PCR, and were then constructed to form the phage surface display human Fab(IgEVH) library. A plant protein allergen, trichosanthin(TCS), was used to affinity-enrich and to screen the anti-TCS phage HuFab clones from the library. Human IgE(Fab) to TCS were detected.展开更多
Bulblet development is a problem in global lily bulb production and carbohydrate metabolism is a crucial factor. Micropropagation acts as an efficient substitute for faster propagation and can provide a controllable c...Bulblet development is a problem in global lily bulb production and carbohydrate metabolism is a crucial factor. Micropropagation acts as an efficient substitute for faster propagation and can provide a controllable condition to explore bulb growth. The present study was conducted to investigate the effects of humic acid (HA) on bulblet swelling and the carbohydrate metabolic pathway in Li/ium Oriental Hybrids 'Sorbonne' under in vitro conditions. HA greatly promoted bulblet growth at 0.2, 2.0, and 20.0 mg/L, and pronounced increases in bulblet sucrose, total soluble sugar, and starch content were observed for higher HA concentrations (_〉2.0 mg/L) within 45 d after transplanting (DAT). The activities of three major starch synthetic enzymes (including adenosine 5'-diphosphate glucose pyro- phosphorylase, granule-bound starch synthase, and soluble starch synthase) were enhanced dramatically after HA application especially low concentration HA (LHA), indicating a quick response of starch metabolism. However, higher doses of HA also caused excessive aboveground biomass accumulation and inhibited root growth. Accordingly, an earlier carbon starvation emerged by observing evident starch degradation. Relative bulblet weight gradually decreased with increased HA doses and thereby broke the balance between the source and sink. A low HA concentration at 0.2 mg/L performed best in both root and bulblet growth. The number of roots and root length peaked at 14.5 and 5.75 cm respectively. The fresh bulblet weight and diameter reached 468 mg (2.9 times that under the control treatment) and 11.68 mm, respectively. Further, sucrose/starch utilization and conversion were accelerated and carbon famine was delayed as a result with an average relative bulblet weight of 80.09%. To our knowledge, this is the first HA application and mechanism research into starch metabolism in both in vitro and in vivo condition in bulbous crops.展开更多
High-capacity anode materials,such as SiO and Si/C,are considered promising candidates for high-energydensity lithium-ion batteries.However,the low initial Coulombic efficiency of these anode materials induced by side...High-capacity anode materials,such as SiO and Si/C,are considered promising candidates for high-energydensity lithium-ion batteries.However,the low initial Coulombic efficiency of these anode materials induced by side reactions(forming Li_(2)O and lithium silicate)and the formation of solid electrolyte interface film reduces the active Liions and causes low-discharge capacity.Adding a Li-compensation material in the cathode or anode is an effective strategy to overcome this problem.The most used Li-compensation material is the stabilized lithium metal powder.However,this strategy has high safety risks,high costs,and is challenging to quantify.Herein,the Li-compensation material of Li_(6)CoO_(4) is synthesized and investigated.The preparation conditions,stability in the air,delithiation mechanism,and structural transformation are analyzed and discussed.Electrochemical tests reveal that the discharge capacity and capacity retention of the full pouch cells(3-Ah)with Li_(6)CoO_(4) additive is significantly improved.Also,the reason for such improvement is investigated.This work provides an effective strategy of Li-compensating technology to enhance the electrochemical performance of lithium-ion batteries.展开更多
文摘Background:During the production of tea,tea dust has been viewed as a risk factor that may cause the suffering of changes of lung function and respiratory symptoms.Objective:To review whether the occupational exposure to tea dust may lead to the changes of lung function and respiratory symptoms.Methods:We searched the literatures in March 2012 in the databases of Medline,EMBase,Web of Science,Wan-Fang Database and CNKI.The search condition is"(herb OR tea)AND(lung function OR ventilatory function OR pulmonary function OR spirometric measurement)".The paper should be pubished in Enghish or Chinese,with internal or external controls.Results:Totally 17 papers were included.Both acute and chronic changes of lung function were referred.In addition,the respiratory symptoms such as cough,tightness and etc.were also reported and discussed.Conclusion:We concluded that the exposure of tea dust may lead to the changes of lung function and respiratory symptoms.
文摘Allergen specific IgE response is the major cause of immediate hypersensitivity. However the number of IgEproducing B cells and the amount of IgE, especially the specific IgE, are so low, it greatly impedes the study of the allergic-specifc antibody responses. Here we report the construction of a normal human IgE combinatorial library The repertoire of IgE VH genes and of K genes were separately amplified from normal human peripheral blood lymphocytes through RT-PCR, and were then constructed to form the phage surface display human Fab(IgEVH) library. A plant protein allergen, trichosanthin(TCS), was used to affinity-enrich and to screen the anti-TCS phage HuFab clones from the library. Human IgE(Fab) to TCS were detected.
基金Project supported by the National High-Tech R&D Program(863) of China(No.2011AA100208)the Zhejiang Provincial Natural Science Foundation of China(No.LY12C15003)
文摘Bulblet development is a problem in global lily bulb production and carbohydrate metabolism is a crucial factor. Micropropagation acts as an efficient substitute for faster propagation and can provide a controllable condition to explore bulb growth. The present study was conducted to investigate the effects of humic acid (HA) on bulblet swelling and the carbohydrate metabolic pathway in Li/ium Oriental Hybrids 'Sorbonne' under in vitro conditions. HA greatly promoted bulblet growth at 0.2, 2.0, and 20.0 mg/L, and pronounced increases in bulblet sucrose, total soluble sugar, and starch content were observed for higher HA concentrations (_〉2.0 mg/L) within 45 d after transplanting (DAT). The activities of three major starch synthetic enzymes (including adenosine 5'-diphosphate glucose pyro- phosphorylase, granule-bound starch synthase, and soluble starch synthase) were enhanced dramatically after HA application especially low concentration HA (LHA), indicating a quick response of starch metabolism. However, higher doses of HA also caused excessive aboveground biomass accumulation and inhibited root growth. Accordingly, an earlier carbon starvation emerged by observing evident starch degradation. Relative bulblet weight gradually decreased with increased HA doses and thereby broke the balance between the source and sink. A low HA concentration at 0.2 mg/L performed best in both root and bulblet growth. The number of roots and root length peaked at 14.5 and 5.75 cm respectively. The fresh bulblet weight and diameter reached 468 mg (2.9 times that under the control treatment) and 11.68 mm, respectively. Further, sucrose/starch utilization and conversion were accelerated and carbon famine was delayed as a result with an average relative bulblet weight of 80.09%. To our knowledge, this is the first HA application and mechanism research into starch metabolism in both in vitro and in vivo condition in bulbous crops.
基金supported by the National Key R&D Program of China(2016YFB0100500)the Young Elite Scientists Sponsorship Program by Tianjin(TJSQNTJ-2017-05)。
文摘High-capacity anode materials,such as SiO and Si/C,are considered promising candidates for high-energydensity lithium-ion batteries.However,the low initial Coulombic efficiency of these anode materials induced by side reactions(forming Li_(2)O and lithium silicate)and the formation of solid electrolyte interface film reduces the active Liions and causes low-discharge capacity.Adding a Li-compensation material in the cathode or anode is an effective strategy to overcome this problem.The most used Li-compensation material is the stabilized lithium metal powder.However,this strategy has high safety risks,high costs,and is challenging to quantify.Herein,the Li-compensation material of Li_(6)CoO_(4) is synthesized and investigated.The preparation conditions,stability in the air,delithiation mechanism,and structural transformation are analyzed and discussed.Electrochemical tests reveal that the discharge capacity and capacity retention of the full pouch cells(3-Ah)with Li_(6)CoO_(4) additive is significantly improved.Also,the reason for such improvement is investigated.This work provides an effective strategy of Li-compensating technology to enhance the electrochemical performance of lithium-ion batteries.