暴露高原不同时间人体血清应激肽和心肌酶改变

目的 探讨进驻高原不同时间人体血清应激肽和心肌酶变化的规律与低氧环境的相关性。方法 平原对照组 3 0例 ,驻成都某部汉族新兵 ,年龄 18～ 2 0岁 ,均未入过藏 ;进藏 3d组 3 0例 ,为从成都空运至拉萨 (海拔 3 65 8m )的汉族新兵 ,年龄 18～ 2 0岁 ,皆为首次入藏 ;移居高原组 3 0例 ,为驻拉萨某部老兵 (驻拉萨 1年左右 ) ,汉族 ,年龄 19～ 2 2岁 ;世居高原组 3 0例 ,拉萨市某中学高三学生 ,藏族 ,年龄 17～ 2 0岁 ;所有受检者均为健康男性。分别检测各组血清低氧应激肽和心肌酶含量 ,并与平原对照组作对比分析。结果 进藏第 3天血清低氧应激肽、CK、LDH、HBDH较平原对照组显著增高 (P <0 .0 1) ,移居高原组与平原对照组比较 ,其血清应激肽水平和AST、CK、LDH、HBDH活性显著增高 (P <0 .0 5 ) ;而移居高原组与进藏 3d组比较 ,其血清应激肽水平显著下降 (P <0 .0 1) ,而血清CK、LDH、HBDH活性无统计学差异 (P >0 .0 5 ) ,世居高原组血清CK、LDH、HBDH活性显著降低 (P <0 .0 1) ;世居高原组与平原对照组血清应激肽水平无统计学差异 (P >0 .0 5 ) ,但藏族世居高原组血清应激肽水平显著低于汉族进藏 3d组 (P <0 .0 1)和汉族移居高原组 (P <0 .0 5 ) ,世居高原组血清CK、LDH、HBDH活性显著低于移居高原组 (

急进高原低氧应激肽氢谱特征及含量变化分析

目的 分析血清低氧应激肽的特征和健康成年人急进高原前后血清低氧应激肽的改变 ,探讨其在高原病治疗和预后中的作用。方法 采用质谱和核磁波谱方法鉴定高原病患者体内存在特征性的应激肽 ,采集 40例高原世居健康者为对照 ,40例健康成年人急进高原前 ,急进高原后 2 4h ,72h ,1周后血清 ,用化学显色试剂对应激肽含量进行测定。结果 40例世居对照组应激肽含量为 72 .9± 9.8mg/L ,健康成年人急进高原前、急进高原后 2 4h ,72h ,1周应激肽含量分别为 73 .5± 11.7mg/L ,90 .8± 10 .3mg/L ,91.7± 10 .1mg/L ,82 .8± 9.5mg/L ,健康成年人急进高原前与急进高原后 2 4h及72h之间有显著性差异 (P <0 .0 5 ) ,急进高原后 2 4h人群与健康世居组之间有显著性差异 (P <0 .0 5 ) ,急进高原后 1周与急进高原后 2 4h之间有显著性差异 (P <0 .0 5 )。结论 应激肽作为一种特殊的应激蛋白 ,在急进高原前后有较大的改变 ,具有一定的价值。

低氧应激肽氢谱特征及急进高原的含量变化分析

目的分析血清低氧应激肽的特征和健康成年人急进高原前后血清低氧应激肽的改变,探讨其在高原病治疗和预后的作用。方法采用质谱和核磁波谱方法鉴定高原病患者体内存在特征性的应激肽,采集40例高原世居健康者为对照组,以40例健康成年人急进高原前、急进高原后24、72h及1周后血清,用化学显色试剂对应激肽含量进行测定。结果40例世居对照组应激肽含量为(729±98)mg/L;实验组健康成年人急进高原前、急进高原后24、72h和1周后应激肽含量分别为(735±117)mg/L、(908±103)mg/L、(917±101)mg/L和(828±95)mg/L。健康成年人急进高原前与急进高原后24h及72h之间有显著性差异(P<005),急进高原后24h人群与健康世居组之间有显著性差异(P<005),急进高原后1周与急进高原后24h之间有显著性差异(P<005)。结论应激肽作为一种特殊的应激蛋白,在急进高原前后有较大的改变,具有一定的检测价值。

STUDY ON THE ANTI-TUMOR EFFICACY INDUCED BY HEAT SHOCK PROTEIN 70-PEPTIDE COMPLEXES DERIVED FROM TUMOR CELLS

OBJECTIVE: To study the efficacy and explore the mechanism of the anti-tumor immunity elicited by heat shock protein 70-peptide complexes (HSP70-PC) derived from tumor cells. METHODS: Cells culture, flow cytometric analysis, affinity chromatography for protein purification, SDS-PAGE, Western-blotting and animal experiment were used. RESULTS: HSP70-PC immunization rendered protective effect to both naive tumorl-bearing mice. All of the naive mice obtained complete resistance to Hcaf cell attack; 40% of the tumor-bearing mice survived for over 90 days, whereas the mice of control group died within 2 weeks (P

Relationship between oxidative stress and hepatic glutathione levels in ethanol-mediated apoptosis of polarized hepatic cells

AIM： To investigate the role of reactive oxygen species （ROS） in ethanol-mediated cell death of polarized hepatic （WlF-B） cells.METHODS： In this work, WIF-B cultures were treated with pyrazole （inducer of cytochrome P4502E1, CYP2E1） and/or L-buthionine sulfoximine （BSO）, a known inhibitor of hepatic glutathione （GSH）, followed by evaluation of ROS production, antioxidant levels, and measures of cell injury （apoptosis and necrosis）.RESULTS： The results revealed that ethanol treatment alone caused a significant two-fold increase in the activation of caspase-3 as well as a similar doubling in ROS. When the activity of the CYP2E1 was increased by pyrazole pretreatment, an additional two-fold elevation in ROS was detected. However, the CYP2EIrelated ROS elevation was not accompanied with a correlative increase in apoptotic cell injury, but rather was found to be associated with an increase in necrotic cell death. Interestingly, when the thiol status of the cells was manipulated using BSO, the ethanol-induced activation of caspase-3 was abrogated. Additionally, ethanol-treated cells displayed enhanced susceptibility to Fas-mediated apoptosis that was blocked by GSH depletion as a result of diminished caspase-8 activity.CONCLUSION： Apoptotic cell death induced as a consequence of ethanol metabolism is not completely dependent upon ROS status but is dependent on sustained GSH levels,

Identification and distribution of gonadotropin-releasing hormone-like peptides in the brain of horseshoe crab Tachypleus tridentatus

Gonadotropin-releasing hormone （GnRH） is a crucial peptide for the regulation of reproduction. Using immunological techniques, we investigated the presence of GnRH in horseshoe crab Tachypleus tridentatus. Octopus GnRH-like immunoreactivity, tunicate GnRH-like immunoreactivity, and lamprey GnRH-I-like immunoreactivity were detected in the neurons and fibers of the protocerebrum. However, no mammal GnRH-like immunoreactivity or lamprey GnRH-LII-like immunoreactivity was observed. Our results suggest that a GnRH-like factor, an ancient peptide, existed in the brain of T. tridentatus and may be involved in the reproductive endocrine system.

Toxic Dinoflagellate Alexandrium tamarense Induces Oxidative Stress and Apoptosis in Hepatopancreas of Shrimp(Fenneropenaeus chinensis)

This study investigated the inductive effect ofAlexandrium tamarense, a toxic dinoflagellate producing paralytic shell- fish poison, on oxidative stress and apoptosis in hepatopancreas of Chinese shrimp, Fenneropenaeus chinensis. The individuals of E chinensis were exposed to 200 and 1000 cells mL-1 of A. tamarense with their superoxide dismutase （SOD）, glutathione S-transferase （GST） activities, malonyldialdehyde （MDA） concentration, and caspase gene （FcCasp） expression in hepatopancreas determined at 12, 24, 48, 72 and 96 h. In addition, apoptosis in hepatopancreas of E chinensis at 96 h after exposure was determined through terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） assay. The hepatopancreatic SOD and GST activities of F. chinensis exposed to 1000 cells mL-1 ofA. tamarense showed a bell-shaped response to exposure time. The hepatopancreatic MDA concentration ofF. chinensis exposed to 1000 cellsmL-1 ofA. tamarense increased gradually from 48 to 96h, and such a trend corresponded to the decrease of GST activity. The hepatopancreatic FcCasp transcript abundance of F. chinensis exposed to 1000 cells mL-1 ofA. tamarense was positively and linearly correlated to MDA concentration. Results of TUNEL assay showed that exposure to 1000 cells mL-1 of A. tamarense induced apoptosis in the hepatopancreas of E chinensis. Our study revealed that A. tamarense exposure influenced the antioxidative status ofF. chinensis and caused lipid peroxidation and apoptosis in the hepatopancreas of shrimp.