The development of unconventional resources, such as shale gas and tight sane gas, requires the integration of multi-disciplinary knowledge to resolve many engineering problems in order to achieve economic production ...The development of unconventional resources, such as shale gas and tight sane gas, requires the integration of multi-disciplinary knowledge to resolve many engineering problems in order to achieve economic production levels. The reservoir heterogeneit3 revealed by different data sets, such as 3D seismic and microseismic data, can more full3 reflect the reservoir properties and is helpful to optimize the drilling and completioT programs. First, we predict the local stress direction and open or close status of the natura fractures in tight sand reservoirs based on seismic curvature, an attribute that reveals reservoi heterogeneity and geomechanical properties. Meanwhile, the reservoir fracture network is predicted using an ant-tracking cube and the potential fracture barriers which can affec hydraulic fracture propagation are predicted by integrating the seismic curvature attribute anc ant-tracking cube. Second, we use this information, derived from 3D seismic data, to assis in designing the fracture program and adjusting stimulation parameters. Finally, we interpre the reason why sand plugs will occur during the stimulation process by the integration of 3E seismic interpretation and microseismic imaging results, which further explain the hydraulic fracure propagation controlling factors and open or closed state of natural fractures in tigh sand reservoirs.展开更多
Pretreatment on vegetable and fruit is a key chain of drying process. The research analyzed mechanisms and applications of different pretreatments such as blanching, freezing, liquid glucose pretreatment, NaCI dipping...Pretreatment on vegetable and fruit is a key chain of drying process. The research analyzed mechanisms and applications of different pretreatments such as blanching, freezing, liquid glucose pretreatment, NaCI dipping, and high-voltage elec- tric field, and proposed the development prospect.展开更多
Based on the synchronous joint gravity and magnetic inversion of single interface by Pilkington and the need of revealing Cenozoic and crystalline basement thickness in the new round of oil-gas exploration, we propose...Based on the synchronous joint gravity and magnetic inversion of single interface by Pilkington and the need of revealing Cenozoic and crystalline basement thickness in the new round of oil-gas exploration, we propose a joint gravity and magnetic inversion methodfor two-layer models by concentrating on the relationship between the change of thicknessI and position of the middle layer and anomaly and discuss the effects of the key parameters. Model tests and application to field data show the validity of this method.展开更多
This paper focus on the ratoon rice, which with the features of south Chi-na paddy cultivation technology. First, the development of the technology research on ratoon rice in southwest ecotope of China was briefly rev...This paper focus on the ratoon rice, which with the features of south Chi-na paddy cultivation technology. First, the development of the technology research on ratoon rice in southwest ecotope of China was briefly reviewed. Second, the key technical measures of the ratoon rice production were described in detail. At last, the application effect of the ratoon rice production technology was described accu-rately, which based on the production practice in recent years. The purpose of this article is promoting the research and extension of the ratoon rice technology more widely in world’s main rice-producing areas, especial y in the suitable area where the light and heat resources are rich.展开更多
The paper analyzes the definite place of industrial building in the history of urban development. Due to the development of urban economy and the transition of the traditional industrial structure, many historic citie...The paper analyzes the definite place of industrial building in the history of urban development. Due to the development of urban economy and the transition of the traditional industrial structure, many historic cities and towns both at home and abroad have witnessed a large scale "demolition" and abandonment of industrial buildings in urban renewal during last 30 years. Consequently, it has been leading to the discontinuity of urban cultural and historic context. The paper discusses and expounds the clas...展开更多
[Objective] The aim was to isolate and identify a taxol-producing endophytic fungus from Taxus media. [Method] 32 strains of endophytic fungi were identified form the inner bark of T. media,and their fermentation prod...[Objective] The aim was to isolate and identify a taxol-producing endophytic fungus from Taxus media. [Method] 32 strains of endophytic fungi were identified form the inner bark of T. media,and their fermentation products were detected by high performance liquid chromatography (HPLC). [Result] Through the screening,a strain of taxol-producing endophytic fungi M57 was obtained,which could produce 45-50 μg/L of taxol,and M57 was defined as Rhizopus sp. through morphological observation and 18S rDNA sequence analysis. [Conclusion] The finding of Rhizopus sp. M57 provided a promising strain for producing taxol with taxol-producing fungi fermentation process.展开更多
Aim: To compare the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia, and to study the effect of humidity and heat on the content of nucleosides. Methods: The contents of nucleos...Aim: To compare the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia, and to study the effect of humidity and heat on the content of nucleosides. Methods: The contents of nucleosides were determined by using high performance capillary electrophoresis (HPCE). Beckman P/ACE System 5010 apparatus equipped with a UV detector and a Beckman untreated fused-silica capillary (57 cm 75 mm, 50 cm effective length) was used. Before sample injection, the capillary was rinsed with 1 molL-1 sodium hydroxide solution and running buffer for 5 min, respectively. A voltage of 20 kV was applied for the separation. Pressure injection was 586 kPa for 6 seconds, and the wavelength of detector was 254 nm. The running time was 20 min at 20 oC. The effect of humidity and heat on the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia was observed for 1, 3, 5 and 10 days at temperature 40 oC, and relative humidity 75%. Results: The content of nucleosides from natural Cordyceps sinensis was higher than that from cultured Cordyceps mycelia. But the contents of nucleosides from freshly collected natural Cordyceps sinensis were very low, even below the limit of quantitation. The contents of nucleosides from natural Cordyceps sinensis were significantly increased by humidity and heat, but this phenomenon was not observed in cultured Cordyceps mycelia. Conclusion: There are differences between the nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia. The nucleosides in natural Cordyceps sinensis may be derived from the degradation of nucleic acids. This implies that adenosine being used for the quality control of natural Cordyceps sinensis may have to be reconsidered.展开更多
The research explored adaptability maize seed coating agent in spring sowing and effects of ultra-fine powder shaped areas in northeast China. The results showed that germination potential and rate both improved aroun...The research explored adaptability maize seed coating agent in spring sowing and effects of ultra-fine powder shaped areas in northeast China. The results showed that germination potential and rate both improved around 5% and the num- ber of root increased by 4% in the treatments with ultra-fine powder shaped maize seed coating agent compared with the control group without seed coating. What's more, the treatments with ultra-fine powder shaped maize seed coating agent took advantages in terms of fresh weight of seeding, stem diameter, and dry weight. It is notable that control effects on underground insects performed the best, with per- centage over 94%, which is more excellent relative to other agents in markets. Therefore, ultra-fine powder shaped maize seed coating agent can be widely applied in spring sowing areas in northeast China, without side effects.展开更多
Based on the transform-domain characteristics of pilot signals,a band suppression filter is used as a transform-domain filter to restrain the interference of noise in channel estimation.The performance effect on chann...Based on the transform-domain characteristics of pilot signals,a band suppression filter is used as a transform-domain filter to restrain the interference of noise in channel estimation.The performance effect on channel estimation for an orthogonal frequency division multiplex (OFDM) system by different energy coefficients in the transform domain and the energy coefficient under the different signal-to-noise ratios (SNR) are also analyzed.A new energy coefficient expression is deduced.It is theoretically proven that dynamically selecting an energy coefficient can significantly improve the performance of channel estimation.Simulation results show that the proposed algorithm can achieve better performance close to the theoretic bounds of perfect channel estimation. The algorithm is adapted to single-input single-output (SISO) OFDM and multi-input multi-output (MIMO) OFDM systems.展开更多
[Objective] This study aimed to provide references for the application of participatory monitoring and evaluation in the development of animal husbandry tech- nology. [Method] With the livelihood improvement program f...[Objective] This study aimed to provide references for the application of participatory monitoring and evaluation in the development of animal husbandry tech- nology. [Method] With the livelihood improvement program for agricultural and pas- toral area in northern Yunnan as the example, the development process of partici- patory animal husbandry was overviewed. And the monitoring and evaluation pro- cesses of participatory animal husbandry were introduced and analyzed. [Result] The monitoring and evaluation indicator system of participatory animal husbandry technol- ogy included 4 layers, namely technology, institutional system, personnel ability con- struction and effectiveness, and 11 third-level indicators, namely, technical characteristics, superiority-inferiority comparison, community and farmers, technical personnel, project personnel, economic indicators and social indicators. [Conclusion] This study provided the references for the application of participatory monitoring and evaluation in the development of animal husbandry technology.展开更多
For the purpose of engineering development for a new 8-step speed automatic transmission,a simplified dynamic model for this gearbox was established and key parameters which affected the shift quality were analyzed.Ai...For the purpose of engineering development for a new 8-step speed automatic transmission,a simplified dynamic model for this gearbox was established and key parameters which affected the shift quality were analyzed.Aiming at four different shift types,the ideal characteristics of shift clutch and engine control were set up.By using torque estimation method,PI slip control algorithm and engine coordinated control principle,the control model and transmission controller were well developed for three shift phases which included rapid-fill phase,torque phase and inertia phase.The testing environment on the rig and prototype vehicle level was built and the testing results obtained in ultimate condition could verify the accuracy and feasibility of this shift control strategy.The peak jerk during shift process was controlled within ±2 g/s where the smooth gearshift was obtained.The development proposal and algorithm have a high value for engineering application.展开更多
Since the impounding of the Three Gorges Reservoir, the channel of the Yangtze River has become a busy watercourse and the probability of landslide-induced tsunamis has increased. In the case of landslide-induced tsun...Since the impounding of the Three Gorges Reservoir, the channel of the Yangtze River has become a busy watercourse and the probability of landslide-induced tsunamis has increased. In the case of landslide-induced tsunamis in the Three Gorges Reservoir, even after shipping closures in advance, there are still facilities and objects in urgent need of protection within the risk zone of the watercourse, such as wharfs, marine fueling stations, berthed ships. The emergency protection against and decay of landslide-induced tsunamis in inland watercourses is a new challenge. In this study, 37 sets of wave decay experiments were conducted with the hydromechanics numerical method. The wave decay efficiencies of common simple structures including submerged horizontal plate, horizontal plate on the water surface, inclined thin plate and cross-plates in coastal areas were compared and analyzed. Cross-plates structure showed better wave decay capacity than other simple plates. The wave decay performance of cross-plates was related to five modes of energy dissipation and transformation, namely run-up/run-down, overtopping, reflecting, return flow and disturbed wave orbital path. The type of wave had little relation with the decay performance of cross-plates, but a strong correlation with cross-plates structure, especially the height of the vertical emerged plate. The best decay performance was observed when the ratio of wave amplitude to emerged vertical plate height was between 1 and 1.5, which can reduce up to about 8o% of the incoming wave amplitude. Finally, the emergency way of cross-plates applied to the decay of landslide-induced tsunami in river course is discussed. This study provides a conceptual reference for related studies to practice the attenuation of landslide-induced tsunami in reservoirs.展开更多
Promoters are the most important tools to control and regulate the gene expression in synthetic biology and metabolic engineering. The expression of target genes in Escherichia coli is usually controlled by the high-s...Promoters are the most important tools to control and regulate the gene expression in synthetic biology and metabolic engineering. The expression of target genes in Escherichia coli is usually controlled by the high-strength inducible promoter with the result that the abnormally high transcription of these genes creates excessive metabolic load on the host, which decreases product formation. The constitutive expression systems are capable of avoiding these defects. In this study, to enrich the application of constitutive promoters in metabolic engineering, four promoters from the glycolytic pathway of E. coli were cloned and characterized using the enhanced green fluorescent protein as reporter. Among these promoters, Pgap Awas determined as the strongest one, the strength of which was about 8.92% of that of the widely used inducible promoter PT7. This promoter was used to control the expression of heterologous xylose reductase in E. coli for xylitol synthesis so as to verify its function in pathway engineering. The maximum xylitol titer(40.6 g·L-1) produced by engineered E. coli under the control of the constitutive promoter Pgap Awas obviously higher than that under the control of the inducible promoter PT7,indicating the feasibility and superiority of promoter Pgap Ain the metabolic engineering of E. coli.展开更多
文摘The development of unconventional resources, such as shale gas and tight sane gas, requires the integration of multi-disciplinary knowledge to resolve many engineering problems in order to achieve economic production levels. The reservoir heterogeneit3 revealed by different data sets, such as 3D seismic and microseismic data, can more full3 reflect the reservoir properties and is helpful to optimize the drilling and completioT programs. First, we predict the local stress direction and open or close status of the natura fractures in tight sand reservoirs based on seismic curvature, an attribute that reveals reservoi heterogeneity and geomechanical properties. Meanwhile, the reservoir fracture network is predicted using an ant-tracking cube and the potential fracture barriers which can affec hydraulic fracture propagation are predicted by integrating the seismic curvature attribute anc ant-tracking cube. Second, we use this information, derived from 3D seismic data, to assis in designing the fracture program and adjusting stimulation parameters. Finally, we interpre the reason why sand plugs will occur during the stimulation process by the integration of 3E seismic interpretation and microseismic imaging results, which further explain the hydraulic fracure propagation controlling factors and open or closed state of natural fractures in tigh sand reservoirs.
基金Supported by National Natural Science Foundation of China(31360399)~~
文摘Pretreatment on vegetable and fruit is a key chain of drying process. The research analyzed mechanisms and applications of different pretreatments such as blanching, freezing, liquid glucose pretreatment, NaCI dipping, and high-voltage elec- tric field, and proposed the development prospect.
基金Supported by the National Natural Science Foundation of China(Grant No.40674063)National Hi-tech Research and Development Program of China(863Program)(Grant No.2006AA09Z311)
文摘Based on the synchronous joint gravity and magnetic inversion of single interface by Pilkington and the need of revealing Cenozoic and crystalline basement thickness in the new round of oil-gas exploration, we propose a joint gravity and magnetic inversion methodfor two-layer models by concentrating on the relationship between the change of thicknessI and position of the middle layer and anomaly and discuss the effects of the key parameters. Model tests and application to field data show the validity of this method.
基金Supported by Chinese Agriculture Special Scientific Research(200903002)The SpecialFunds for Major Chongqing Science and Technology Projects(cstc2012ggB8005)The Special Funds for Chongqing Agriculture Scientific Research and Extention~~
文摘This paper focus on the ratoon rice, which with the features of south Chi-na paddy cultivation technology. First, the development of the technology research on ratoon rice in southwest ecotope of China was briefly reviewed. Second, the key technical measures of the ratoon rice production were described in detail. At last, the application effect of the ratoon rice production technology was described accu-rately, which based on the production practice in recent years. The purpose of this article is promoting the research and extension of the ratoon rice technology more widely in world’s main rice-producing areas, especial y in the suitable area where the light and heat resources are rich.
文摘The paper analyzes the definite place of industrial building in the history of urban development. Due to the development of urban economy and the transition of the traditional industrial structure, many historic cities and towns both at home and abroad have witnessed a large scale "demolition" and abandonment of industrial buildings in urban renewal during last 30 years. Consequently, it has been leading to the discontinuity of urban cultural and historic context. The paper discusses and expounds the clas...
基金Supported by National Natural Science Foundation of China(20776058)New Century Training Programme Foundation for the Talents by the State Education Commission (NCET-06-0646)~~
文摘[Objective] The aim was to isolate and identify a taxol-producing endophytic fungus from Taxus media. [Method] 32 strains of endophytic fungi were identified form the inner bark of T. media,and their fermentation products were detected by high performance liquid chromatography (HPLC). [Result] Through the screening,a strain of taxol-producing endophytic fungi M57 was obtained,which could produce 45-50 μg/L of taxol,and M57 was defined as Rhizopus sp. through morphological observation and 18S rDNA sequence analysis. [Conclusion] The finding of Rhizopus sp. M57 provided a promising strain for producing taxol with taxol-producing fungi fermentation process.
文摘Aim: To compare the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia, and to study the effect of humidity and heat on the content of nucleosides. Methods: The contents of nucleosides were determined by using high performance capillary electrophoresis (HPCE). Beckman P/ACE System 5010 apparatus equipped with a UV detector and a Beckman untreated fused-silica capillary (57 cm 75 mm, 50 cm effective length) was used. Before sample injection, the capillary was rinsed with 1 molL-1 sodium hydroxide solution and running buffer for 5 min, respectively. A voltage of 20 kV was applied for the separation. Pressure injection was 586 kPa for 6 seconds, and the wavelength of detector was 254 nm. The running time was 20 min at 20 oC. The effect of humidity and heat on the contents of nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia was observed for 1, 3, 5 and 10 days at temperature 40 oC, and relative humidity 75%. Results: The content of nucleosides from natural Cordyceps sinensis was higher than that from cultured Cordyceps mycelia. But the contents of nucleosides from freshly collected natural Cordyceps sinensis were very low, even below the limit of quantitation. The contents of nucleosides from natural Cordyceps sinensis were significantly increased by humidity and heat, but this phenomenon was not observed in cultured Cordyceps mycelia. Conclusion: There are differences between the nucleosides from natural Cordyceps sinensis and cultured Cordyceps mycelia. The nucleosides in natural Cordyceps sinensis may be derived from the degradation of nucleic acids. This implies that adenosine being used for the quality control of natural Cordyceps sinensis may have to be reconsidered.
基金Supported by Key Projects in the National Science&Technology Pillar Program~~
文摘The research explored adaptability maize seed coating agent in spring sowing and effects of ultra-fine powder shaped areas in northeast China. The results showed that germination potential and rate both improved around 5% and the num- ber of root increased by 4% in the treatments with ultra-fine powder shaped maize seed coating agent compared with the control group without seed coating. What's more, the treatments with ultra-fine powder shaped maize seed coating agent took advantages in terms of fresh weight of seeding, stem diameter, and dry weight. It is notable that control effects on underground insects performed the best, with per- centage over 94%, which is more excellent relative to other agents in markets. Therefore, ultra-fine powder shaped maize seed coating agent can be widely applied in spring sowing areas in northeast China, without side effects.
文摘Based on the transform-domain characteristics of pilot signals,a band suppression filter is used as a transform-domain filter to restrain the interference of noise in channel estimation.The performance effect on channel estimation for an orthogonal frequency division multiplex (OFDM) system by different energy coefficients in the transform domain and the energy coefficient under the different signal-to-noise ratios (SNR) are also analyzed.A new energy coefficient expression is deduced.It is theoretically proven that dynamically selecting an energy coefficient can significantly improve the performance of channel estimation.Simulation results show that the proposed algorithm can achieve better performance close to the theoretic bounds of perfect channel estimation. The algorithm is adapted to single-input single-output (SISO) OFDM and multi-input multi-output (MIMO) OFDM systems.
基金Supported by the Canadian International Development Research Center(IDRC)~~
文摘[Objective] This study aimed to provide references for the application of participatory monitoring and evaluation in the development of animal husbandry tech- nology. [Method] With the livelihood improvement program for agricultural and pas- toral area in northern Yunnan as the example, the development process of partici- patory animal husbandry was overviewed. And the monitoring and evaluation pro- cesses of participatory animal husbandry were introduced and analyzed. [Result] The monitoring and evaluation indicator system of participatory animal husbandry technol- ogy included 4 layers, namely technology, institutional system, personnel ability con- struction and effectiveness, and 11 third-level indicators, namely, technical characteristics, superiority-inferiority comparison, community and farmers, technical personnel, project personnel, economic indicators and social indicators. [Conclusion] This study provided the references for the application of participatory monitoring and evaluation in the development of animal husbandry technology.
基金Project(51105017) supported by the National Natural Science Foundation of ChinaProject(2011BAG09B00) supported by the National Science and Technology Support Program of ChinaProject(2010DFB80020) supported by the Technology Major Project of the Ministry of Science and Technology of China
文摘For the purpose of engineering development for a new 8-step speed automatic transmission,a simplified dynamic model for this gearbox was established and key parameters which affected the shift quality were analyzed.Aiming at four different shift types,the ideal characteristics of shift clutch and engine control were set up.By using torque estimation method,PI slip control algorithm and engine coordinated control principle,the control model and transmission controller were well developed for three shift phases which included rapid-fill phase,torque phase and inertia phase.The testing environment on the rig and prototype vehicle level was built and the testing results obtained in ultimate condition could verify the accuracy and feasibility of this shift control strategy.The peak jerk during shift process was controlled within ±2 g/s where the smooth gearshift was obtained.The development proposal and algorithm have a high value for engineering application.
基金funded by the National Natural Science Foundation Project of China(41372321)China Geological Survey Project(121201009000150018)
文摘Since the impounding of the Three Gorges Reservoir, the channel of the Yangtze River has become a busy watercourse and the probability of landslide-induced tsunamis has increased. In the case of landslide-induced tsunamis in the Three Gorges Reservoir, even after shipping closures in advance, there are still facilities and objects in urgent need of protection within the risk zone of the watercourse, such as wharfs, marine fueling stations, berthed ships. The emergency protection against and decay of landslide-induced tsunamis in inland watercourses is a new challenge. In this study, 37 sets of wave decay experiments were conducted with the hydromechanics numerical method. The wave decay efficiencies of common simple structures including submerged horizontal plate, horizontal plate on the water surface, inclined thin plate and cross-plates in coastal areas were compared and analyzed. Cross-plates structure showed better wave decay capacity than other simple plates. The wave decay performance of cross-plates was related to five modes of energy dissipation and transformation, namely run-up/run-down, overtopping, reflecting, return flow and disturbed wave orbital path. The type of wave had little relation with the decay performance of cross-plates, but a strong correlation with cross-plates structure, especially the height of the vertical emerged plate. The best decay performance was observed when the ratio of wave amplitude to emerged vertical plate height was between 1 and 1.5, which can reduce up to about 8o% of the incoming wave amplitude. Finally, the emergency way of cross-plates applied to the decay of landslide-induced tsunami in river course is discussed. This study provides a conceptual reference for related studies to practice the attenuation of landslide-induced tsunami in reservoirs.
基金Supported by the National High Technology Research and Development Program of China(863 Program)(2012AA02A704)the Major State Basic Research Development Program of China(973 Program)(2013CB733900)+1 种基金the National Natural Science Foundation of China(21176028,21376028)the National Research Foundation for the Doctoral Program of Higher Education of China(20121101110050)
文摘Promoters are the most important tools to control and regulate the gene expression in synthetic biology and metabolic engineering. The expression of target genes in Escherichia coli is usually controlled by the high-strength inducible promoter with the result that the abnormally high transcription of these genes creates excessive metabolic load on the host, which decreases product formation. The constitutive expression systems are capable of avoiding these defects. In this study, to enrich the application of constitutive promoters in metabolic engineering, four promoters from the glycolytic pathway of E. coli were cloned and characterized using the enhanced green fluorescent protein as reporter. Among these promoters, Pgap Awas determined as the strongest one, the strength of which was about 8.92% of that of the widely used inducible promoter PT7. This promoter was used to control the expression of heterologous xylose reductase in E. coli for xylitol synthesis so as to verify its function in pathway engineering. The maximum xylitol titer(40.6 g·L-1) produced by engineered E. coli under the control of the constitutive promoter Pgap Awas obviously higher than that under the control of the inducible promoter PT7,indicating the feasibility and superiority of promoter Pgap Ain the metabolic engineering of E. coli.