运用了Min Run Equirelicated Res IV析因设计、爬陡坡试验以及中心复合响应面设计对碱性蛋白酶酶解冷榨花生粕蛋白制备活性多肽工艺进行了优化。析因设计结果和显著性分析发现,[E]和[S]为最重要因素(P<0.01),酶解pH和酶解时间为重...运用了Min Run Equirelicated Res IV析因设计、爬陡坡试验以及中心复合响应面设计对碱性蛋白酶酶解冷榨花生粕蛋白制备活性多肽工艺进行了优化。析因设计结果和显著性分析发现,[E]和[S]为最重要因素(P<0.01),酶解pH和酶解时间为重要因素(P<0.05)。在析因设计、爬陡坡试验设计结果基础上应用中心复合响应面设计对[E](X1)、[S](X2)和酶解时间(X3)进行了响应面优化分析。响应面优化结果表明,在[E]=4 300 U/g[S],[S]=10.0%,酶解时间为85 min最优条件下,酶解液多肽含量达到最高,为(0.209±0.005)%(n=6),与模型预测值0.207 8%接近,偏差为5.77%。试验表明,酶促水解是制备活性多肽的有效方法。展开更多
The alkaline leaching of arsenic (As2O3) by Na2S, together with its precipitation by Fe2(SO4)3 was studied. Response surface methodology based on central composite design was employed to quantify and qualify the effec...The alkaline leaching of arsenic (As2O3) by Na2S, together with its precipitation by Fe2(SO4)3 was studied. Response surface methodology based on central composite design was employed to quantify and qualify the effect of pertinent factors and to develop statistical models for optimization purposes. Based on the obtained results, 89% of arsenic is removed from the dust under following optimum predicted conditions: Na2S concentration of 100 g/L and solid to liquid ratio of 0.163 g/mL at 80 °C. It is found that solid to liquid ratio and Na2S concentration are the significant factors influencing the leaching process. In the precipitation process, more than 99.93% of arsenic from the leaching solution is removed in the form of amorphous ferric arsenate, at pH 4.8 when Fe3+ to arsenic and H2O2 to arsenic molar ratios are set at 5:1 and 4:1, respectively. Also, Fe3+ to arsenic ratio and pH are the most significant factors, and the interaction between these terms is significant.展开更多
文摘运用了Min Run Equirelicated Res IV析因设计、爬陡坡试验以及中心复合响应面设计对碱性蛋白酶酶解冷榨花生粕蛋白制备活性多肽工艺进行了优化。析因设计结果和显著性分析发现,[E]和[S]为最重要因素(P<0.01),酶解pH和酶解时间为重要因素(P<0.05)。在析因设计、爬陡坡试验设计结果基础上应用中心复合响应面设计对[E](X1)、[S](X2)和酶解时间(X3)进行了响应面优化分析。响应面优化结果表明,在[E]=4 300 U/g[S],[S]=10.0%,酶解时间为85 min最优条件下,酶解液多肽含量达到最高,为(0.209±0.005)%(n=6),与模型预测值0.207 8%接近,偏差为5.77%。试验表明,酶促水解是制备活性多肽的有效方法。
文摘The alkaline leaching of arsenic (As2O3) by Na2S, together with its precipitation by Fe2(SO4)3 was studied. Response surface methodology based on central composite design was employed to quantify and qualify the effect of pertinent factors and to develop statistical models for optimization purposes. Based on the obtained results, 89% of arsenic is removed from the dust under following optimum predicted conditions: Na2S concentration of 100 g/L and solid to liquid ratio of 0.163 g/mL at 80 °C. It is found that solid to liquid ratio and Na2S concentration are the significant factors influencing the leaching process. In the precipitation process, more than 99.93% of arsenic from the leaching solution is removed in the form of amorphous ferric arsenate, at pH 4.8 when Fe3+ to arsenic and H2O2 to arsenic molar ratios are set at 5:1 and 4:1, respectively. Also, Fe3+ to arsenic ratio and pH are the most significant factors, and the interaction between these terms is significant.
