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利用Ⅲ型聚酮合酶CsyB起始单元的底物多样性体内合成csypyrone类化合物 被引量:1
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作者 潘丽霞 朱婧 +3 位作者 王青艳 申乃坤 李亿 杨登峰 《生物工程学报》 CAS CSCD 北大核心 2018年第7期1137-1146,共10页
作为新型Ⅲ型聚酮合酶,米曲霉来源的CsyB能够依次接受一个起始单元为短链脂肪酰辅酶A、一个延伸单元为丙二酰辅酶A和另一个延伸单元为乙酰乙酰辅酶A的3个底物形成短链的csypyrone B1-3。基于CsyB的晶体结构分析,显示它的活性中心存在一... 作为新型Ⅲ型聚酮合酶,米曲霉来源的CsyB能够依次接受一个起始单元为短链脂肪酰辅酶A、一个延伸单元为丙二酰辅酶A和另一个延伸单元为乙酰乙酰辅酶A的3个底物形成短链的csypyrone B1-3。基于CsyB的晶体结构分析,显示它的活性中心存在一个长约16?的能够接受脂肪酰辅酶A结合通道,这个通道很可能能够接受多种底物。为了检测该酶的底物多样性,将CsyB基因导入到存在长链脂肪酰辅酶A前体的大肠杆菌中表达。高效液相结果显示,相比对照菌株,重组菌株产生了一系列长链的csypyrone衍生物。利用紫外可见光特征吸收值和高分辨液相色谱-质谱联用仪对这些新产物作了初步分析。对3个具有羟基的csypyrone产物的结构进行了核磁共振一维谱和二维谱的详细鉴定,确定了其羟基的位置。上述结果显示,CsyB具有广泛的底物特异性,不但可以接受多种长链饱和或不饱和脂肪酰辅酶A,还可以接受具有羟基修饰的长链脂肪酰辅酶A作为底物。 展开更多
关键词 Ⅲ型聚酮合酶 CsyB 底物多样性 体内合成 csypyrone
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槐糖脂发酵底物的多样性 被引量:4
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作者 伏圣秘 张红蕊 陈静 《齐鲁工业大学学报》 CAS 2014年第1期39-43,共5页
槐糖脂是一种糖脂类生物表面活性剂,主要通过一些酵母菌发酵合成并分泌到胞外。随着研究的不断深入,槐糖脂种类不断增多,它们的区别主要表现在其结构及分子组成上,这最终导致了其理化性质和生物活性的不同。发酵底物的多样性是槐糖脂结... 槐糖脂是一种糖脂类生物表面活性剂,主要通过一些酵母菌发酵合成并分泌到胞外。随着研究的不断深入,槐糖脂种类不断增多,它们的区别主要表现在其结构及分子组成上,这最终导致了其理化性质和生物活性的不同。发酵底物的多样性是槐糖脂结构及分子组成差异的最主要的因素。本文主要从碳源和氮源上对发酵底物进行分类综述。 展开更多
关键词 槐糖脂 底物多样性 碳源 氮源
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Macrobenthos Community Structure and Its Relations With Environmental Factors in Three Reservoirs in Tianjin 被引量:1
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作者 姜巨峰 傅志茹 +4 位作者 李文雯 张韦 孟一耕 钱红 李相普 《Agricultural Science & Technology》 CAS 2014年第6期947-953,969,共8页
[Objective] The aim was to research macrobenthos community structure and its relations with environmental factors in three reservoirs. [Method] Benthic diversity and water quality of Yuqiao Reservoir, Beidagang Reserv... [Objective] The aim was to research macrobenthos community structure and its relations with environmental factors in three reservoirs. [Method] Benthic diversity and water quality of Yuqiao Reservoir, Beidagang Reservoir and Er- wangzhuang Reservoir were investigated in May, July and September 2011, respec- tively. []Result] Of all the 14 sampling sites, 22 species of macroinvertebrates were collected, totally, which included 6 species of aquatic insects, 7 species of Mollusca, 2 species of Crustacean, 7 species of Oligochaeta. The species of macroinverte- brates during May, July and September in Yuqiao reservoir were 11, 8 and 10, respectively. The dominant species were Chironomus sinicus, and Monopylephorus limosus. The average density and biomass of the macrobenthos were 132.43 ind./m2 and 50.09 g/m2. Yuqiao reservoir was mostly at status of premediate polluted, according to the Margalef index, Shannon index and Pielou index. The species of during May, July and September in Beidagang reservoir were 5 5 and 5, respectively. The dominant species were Chironomus sinicus, Penaeus vannamei. The average density and biomass of the macrobenthos were 218 ind./m2 and 2.70 g/m2. Beidagang reservoir was mostly at status of heavy polluted, accord- ing to the Margalef index, Shannon-wiener index, Pielou index. The species of during May, July and September in Erwangzhuang reservoir were 3, 5 and 5, respectively. The dominant species were Monopylephorus limosus, Chi- ronomus sinicus. The average density and biomass of the macrobenthos were 104 ind/m2 and 0.20 g/m2. Erwangzhuang reservoir was mostly at status of intermediate polluted, according to the Margalef index, Shannon index, Pielou index. The density of zoobenthos in Yuqiao Reservoir was positively significantly correlated with TP (P〉 0.05), negatively correlated with NH4+-N (P〉0.05), positively correlated with COD (P〈 0.01), not significantly correlated with other environment factors (P〉0.05). There was no significant correlation between biomass of zoobenthos and environment factors. The density of zoobenthos in Beidagang Reservoir with NH4+-N was positively signif- icantly correlated (P〈0.05), not significantly correlated with other aquatic environment factors (P〉0.05). There was no significant correlation between biomass of zoobenthos and environment factors. The density of zoobenthos in Erwangzhuang Reser- voir with NO3-N was positively significantly correlated (P〈0.05), not significant cor- related with other aquatic environment factors (F〉0.05). The biomass of zoobenthos in Erwangzhuang Reservoir with NH4+-N was positively significantly correlated (P〈 0.05), not significant correlated with other aquatic environment factors (P〉0.05). [Conclusion] It is indicated that this three reservoirs were all eutrophied and polluted, and the eurephication extent change of water in three reservoirs is as follows: Beidagang reservoir〉Erwangzhuang reservoir〉Yuqiao reservoir. 展开更多
关键词 MACROBENTHOS Community structure Diversity index Water quality as-sessment Large reservoirs TIANJIN
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Community characteristics of macrobenthos community in Changli Gold Coast National Nature Reserve of Hebei in China 被引量:2
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作者 KOU Cun-hui ZHANG Qing-tian +4 位作者 YU Hang LI De-liang ZHAO Xing-gui ZHANG Peng-ru-yan LIU Xian-bin 《Marine Science Bulletin》 CAS 2017年第2期38-50,共13页
Macrobenthic communities in the surrounding waters of Changli were investigated during spring and summer in2016.Differences in species quantity,abundance and biomass,the dominant species and species diversity index of... Macrobenthic communities in the surrounding waters of Changli were investigated during spring and summer in2016.Differences in species quantity,abundance and biomass,the dominant species and species diversity index of macrobenthos were analyzed.The results showed that58macrobenthos species were identified in spring,and92macrobenthos species were identified in summer.The composition of dominant species seasonally varied;however,most of them were species belonging to Polychaeta.The abundance of macrobenthos in summer was slightly higher than that in spring,while the biomass in summer was significantly smaller than that in spring.Bray-Curtis cluster analysis and multi-dimentional scaling(MDS)analysis indicated that macrobenthic communities were divided into three communities in spring,and two in summer.The abundance-biomass comparison(ABC)curve method was used to monitor the disturbance of environmental pollution for macrobenthic community.The results showed that the macrobenthos in this area received serious disturbance from environmental pollution. 展开更多
关键词 Changli MACROBENTHOS DIVERSITY cluster analysis ABC curve method
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Impact of Dredging on Sediment of Krishnapatnam Port, East Coast of India: Implications for Marine Biodiversity
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作者 N. Jayaju B.C. Sundara Raja Reddy K.R. Reddy 《Journal of Environmental Science and Engineering》 2010年第8期66-75,共10页
This paper synthesizes the extent and nature of scientific information about how dredging activities potentially affect habitats and key ecological functions supporting recruitment and sustainability of estuarine and ... This paper synthesizes the extent and nature of scientific information about how dredging activities potentially affect habitats and key ecological functions supporting recruitment and sustainability of estuarine and marine environment. Fourteen samples were collected after dredging (2008) from fixed sampling stations. The impact on community was estimated at species level (Foraminifera, Protozoan, using statistical analysis). The maximum negative effect on benthic foraminifera was reduction by 60%, for species richness and by 50% for diversity. This data were compared with the data obtained before dredging (2006) in a time services spanning 2 years. Its revealed that reestablishment of directly with in less than 3 months of the end of dredging, although affected foraminifera and of physico-chemical substrate characteristic 2 years later there was a considerable improvement of whole faunal community. Statistical treatment was given to the data sets to know the relation among parameters. Before, this type of activity is undertaken, each case should be studied regarding viability, the environmental medium where it will take place, the best time of year, and the type of dredging to be used. Small-patch dredging operations are proposed when ever possible, since they allow a quick readjustment of the initial sediment structure and benthic foraminifera. These findings will help to underpin improved planning of management strategies for dredging operations in India and other countries. 展开更多
关键词 Foraminifera. sediment DREDGING ecological impact krishnapatnam port east coast of India.
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苯酚羟化酶基因工程菌表达优化及特性研究 被引量:6
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作者 曹湘禹 黄益养 +5 位作者 时胜男 马桥 李新亮 孔春雷 沈娥 曲媛媛 《环境科学学报》 CAS CSCD 北大核心 2012年第7期1549-1556,共8页
利用表面响应法优化苯酚羟化酶基因工程菌(PH_IND)的生长条件(种龄、接种量)及表达条件(诱导OD600、IPTG浓度、诱导时间、诱导温度),并对其粗酶特性进行考察.实验结果表明,菌株PH_IND的最佳生长条件及表达条件为:种龄7.19h,接种量1.39%... 利用表面响应法优化苯酚羟化酶基因工程菌(PH_IND)的生长条件(种龄、接种量)及表达条件(诱导OD600、IPTG浓度、诱导时间、诱导温度),并对其粗酶特性进行考察.实验结果表明,菌株PH_IND的最佳生长条件及表达条件为:种龄7.19h,接种量1.39%,诱导OD6000.54,IPTG浓度0.19mmol·L-1,诱导时间2.37h,诱导温度35℃,此时,苯酚羟化酶最大酶活力为0.6167U·mg-1.粗酶特性分析表明,苯酚羟化酶的最适pH和温度分别为8.0和40℃.Ba2+、Fe3+、Hg2+、Pb2+对苯酚羟化酶有明显促进作用,而Ca2+、Co2+、Cu2+、Mg2+、Mn2+对苯酚羟化酶有抑制作用.底物广谱性实验表明,苯酚羟化酶具有极强的芳香化合物降解能力. 展开更多
关键词 苯酚 苯酚羟化酶 表面响应法 底物多样性
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