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内源性过氧化物酶染色显示微血管及判定血液循环方法的建立与应用 被引量:6
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作者 熊正文 李春光 +1 位作者 李自强 王颜忠 《中国组织化学与细胞化学杂志》 CAS CSCD 1993年第2期146-146,共1页
1991年以来,我们开展了利用血液中的内源性过氧化物酶染色显示微血管的系列应用研究,取得了一些结果。 1.方法建立:利用血细胞和它释放到血浆中的内源性过氧化物酶,与底物液中的联苯胺反应产生一种不溶于水,酒精和二甲苯等有机溶剂的联... 1991年以来,我们开展了利用血液中的内源性过氧化物酶染色显示微血管的系列应用研究,取得了一些结果。 1.方法建立:利用血细胞和它释放到血浆中的内源性过氧化物酶,与底物液中的联苯胺反应产生一种不溶于水,酒精和二甲苯等有机溶剂的联苯胺棕沉淀,将血管显示出来,当金属镍离子存在时可增强其敏感性。由于该酶存在于血细胞和血浆中,故可根据微血管的数量和颜色深浅定量或半定量地判定组织器官活体时的血液循环状况。 展开更多
关键词 循环 联苯胺反应 底物液 镍离子 于水 组织形态 皮瓣移植术 有机溶剂 双标记 医用微波仪
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应用ELISA技术提高乙型肝炎病毒检测准确性的初探
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作者 朱淼清 关荣娟 《临床检验杂志》 CAS CSCD 北大核心 1994年第S1期157-157,共1页
应用ELISA技术提高乙型肝炎病毒检测准确性的初探朱淼清,关荣娟(辽宁省沈阳市苏家屯区中医医院,110101)1包被工作中应注意的问题新板原则上不需处理,如有灰尘可用吹风除掉或用蒸馏水冲洗40℃烘干备用,切忌用毛刷或... 应用ELISA技术提高乙型肝炎病毒检测准确性的初探朱淼清,关荣娟(辽宁省沈阳市苏家屯区中医医院,110101)1包被工作中应注意的问题新板原则上不需处理,如有灰尘可用吹风除掉或用蒸馏水冲洗40℃烘干备用,切忌用毛刷或棉签等洗刷,也不宜用碱性洗涤剂,否... 展开更多
关键词 检测准确性 ELISA技术 乙型肝炎病毒 中医医院 蒸馏水冲洗 反应板 朱淼 终止 底物液 抗原抗体
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木聚糖酶酶活测定的几点体会 被引量:4
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作者 罗长辉 陈冰 +2 位作者 李书明 王银东 吴世林 《广东饲料》 2011年第5期37-38,共2页
本文总结了在木聚糖酶(制剂)酶活实际测定过程中底物种类、底物配制方式和酶液稀释倍数对测定结果的影响。
关键词 木聚糖酶酶活测定稀释倍数
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Analysis of influencing factors on suction capacity in seabed natural gas hydrate by cutter-suction exploitation 被引量:2
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作者 XU Hai-liang KONG Wei-yang HU Wen-gang 《Journal of Central South University》 SCIE EI CAS CSCD 2018年第12期2883-2895,共13页
The mathematical and simulation models of working head in the deep-sea working environment were built to analyze the effects of cutter-suction flow,cutter-head rotating speed,cutting depth and suction port position on... The mathematical and simulation models of working head in the deep-sea working environment were built to analyze the effects of cutter-suction flow,cutter-head rotating speed,cutting depth and suction port position on the cutter-suction capacity.The efficiency of the cutter-suction is analyzed based on the analysis of the variation law of the solid-phase volume fraction of the flow field,the variation law of the velocity distribution in the flow field and the distribution law of the solid-phase concentration.The results show that the increase of cutter-suction flow can significantly improve the cutter-suction efficiency when it is less than1000m3/h.However,when it is more than1000m3/h,it is helpless.When the cutter-head rotate speed is within the range of10–25r/min,the cutter-suction efficiency stabilizes at about95%.While the speed is greater than25r/min,the cutter-suction efficiency decreases sharply with the increase of cutter-head rotate speed.With the increase of cutting depth,the cutter-suction efficiency first increases and then remains stable and finally decreases.The cutter-suction efficiency remains at about94%when the suction port position deviation ranges from0°to30°,but it has a sharply reduction when the deviation angle is more than30°. 展开更多
关键词 seabed natural gas hydrate working head solid-liquid two-phase flow cutter-suction capacity influencing factor
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The Influence of Seafloor Hydrothermal Activity on Major and Trace Elements of the Sediments From the South Mid-Atlantic Ridge 被引量:4
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作者 HUANG Xin CHEN Shuai +2 位作者 ZENG Zhigang PU Xiaoqiang HOU Qinghua 《Journal of Ocean University of China》 SCIE CAS CSCD 2017年第5期775-780,共6页
Sediment samples obtained from the South Mid-Atlantic Ridge were analyzed for the major and trace elements by inductively coupled plasma atomic emission spectroscopy and inductively coupled plasma mass spectrometry. R... Sediment samples obtained from the South Mid-Atlantic Ridge were analyzed for the major and trace elements by inductively coupled plasma atomic emission spectroscopy and inductively coupled plasma mass spectrometry. Results revealed that the contents of elements(e.g., Fe, Mn, Cu, Zn, V, Co) were high in samples 22V-TVG10 and 26V-TVG05 from the sites near the hydrothermal areas, and low in sample 22V-TVG14, which was collected far from the hydrothermal areas. The contents of Ca, Sr and Ba in the samples showed opposite trends. A positive correlation between the concentrations of metallic elements(Cu, Zn, Co, Ni, Pb, V) and Fe in the samples were observed. These results are consistent with chemical evolution of the dispersing hydrothermal plume. 展开更多
关键词 Atlantic hydrothermal inductively metallic Sediment viscosity ocean agate Major opposite
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