Laccase was immobilized on the ceramic-chitosan composite support by using glutaraldehyde as the cross-linking reagent. The immobilization conditions and characterization of the immobilized enzyme were investigated. T...Laccase was immobilized on the ceramic-chitosan composite support by using glutaraldehyde as the cross-linking reagent. The immobilization conditions and characterization of the immobilized enzyme were investigated. The immobilization of laccase was successfully realized when 3.0 mL of 1.25 mg/mL of laccase at a pH value of 4.0 reacted with 0.15 g of ceramic-chitosan composite support(CCCS) at 4 ℃ for 24 h. The immobilized enzyme exhibited a maximum activity at pH 3.0. The optimal temperatures for immobilized enzyme were 25 ℃ and 50 ℃. The K_m value of immobilized laccase for ABTS was 66.64 μmol/L at a pH value of 3.0 at 25 ℃. Compared with free laccase, the thermal, operating and storage stability of immobilized laccase was improved after the immobilization.展开更多
Urbanization is the physical growth of urban areas as a result of global change. As the land cost is increasing tremendously and decreasing availability of good construction site is building up pressure on the enginee...Urbanization is the physical growth of urban areas as a result of global change. As the land cost is increasing tremendously and decreasing availability of good construction site is building up pressure on the engineers to utilize even the poorest site either by providing special type of foundation or by improving ground in urban centres. In this context literature is reviewed for use of landfill site for housing. The site exploration for old dump site was carried out to assess subsoil characteristics. The objective was to evolve strategy for economical feasible ground improvement technique to obtain permissible bearing capacity of 150 kPa and total settlement not more than 50 mm. The tests carried out are load tests with geotextile mat and stone filled wire mess matress. The analysis was attempted to evaluate the soil response and bearing capacity. The site can be used for construction of low rise housing for rehabilitation of displaced persons under TP scheme within city area utilizing old landfill sites.展开更多
文摘Laccase was immobilized on the ceramic-chitosan composite support by using glutaraldehyde as the cross-linking reagent. The immobilization conditions and characterization of the immobilized enzyme were investigated. The immobilization of laccase was successfully realized when 3.0 mL of 1.25 mg/mL of laccase at a pH value of 4.0 reacted with 0.15 g of ceramic-chitosan composite support(CCCS) at 4 ℃ for 24 h. The immobilized enzyme exhibited a maximum activity at pH 3.0. The optimal temperatures for immobilized enzyme were 25 ℃ and 50 ℃. The K_m value of immobilized laccase for ABTS was 66.64 μmol/L at a pH value of 3.0 at 25 ℃. Compared with free laccase, the thermal, operating and storage stability of immobilized laccase was improved after the immobilization.
文摘Urbanization is the physical growth of urban areas as a result of global change. As the land cost is increasing tremendously and decreasing availability of good construction site is building up pressure on the engineers to utilize even the poorest site either by providing special type of foundation or by improving ground in urban centres. In this context literature is reviewed for use of landfill site for housing. The site exploration for old dump site was carried out to assess subsoil characteristics. The objective was to evolve strategy for economical feasible ground improvement technique to obtain permissible bearing capacity of 150 kPa and total settlement not more than 50 mm. The tests carried out are load tests with geotextile mat and stone filled wire mess matress. The analysis was attempted to evaluate the soil response and bearing capacity. The site can be used for construction of low rise housing for rehabilitation of displaced persons under TP scheme within city area utilizing old landfill sites.
