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基于立即早期蛋白IE62建立水痘-带状疱疹病毒感染性滴度检测方法 被引量:5
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作者 潘德全 王玮 +5 位作者 付文锟 蔡琳俐 叶江辉 朱桦 程通(指导) 夏宁邵 《中国免疫学杂志》 CAS CSCD 北大核心 2018年第12期1843-1848,共6页
目的:基于水痘-带状疱疹病毒(VZV)立即早期蛋白IE62与酶联免疫斑点检测技术(ELISpot)建立一种新型的VZV感染性滴度的快速检测方法。方法:应用生物信息学方法设计并合成VZV-IE62蛋白的多肽抗原,牛血清白蛋白偶联后免疫BALB/c小鼠,筛选和... 目的:基于水痘-带状疱疹病毒(VZV)立即早期蛋白IE62与酶联免疫斑点检测技术(ELISpot)建立一种新型的VZV感染性滴度的快速检测方法。方法:应用生物信息学方法设计并合成VZV-IE62蛋白的多肽抗原,牛血清白蛋白偶联后免疫BALB/c小鼠,筛选和制备抗VZV-IE62单克隆抗体,应用Western blot和免疫荧光法等开展抗体性能评价,继而应用酶联免疫斑点技术(ELISpot)结合生物素-亲和素放大法建立新型的VZV感染性滴度检测方法,并与经典空斑计数法进行比较。结果:获得抗VZV-IE62的单克隆抗体1B7,应用于ELISpot方法可特异识别VZV感染后的细胞,以之为基础建立了新型的VZV感染性滴度检测方法。相比经典空斑计数法,该方法可显著缩短检测时间(从5至7 d缩短至32 h)检测结果具有较好的一致性。结论:本研究建立了一种新型的基于VZV立即早期蛋白IE62与酶联免疫斑点技术的VZV感染性滴度检测方法(VZV-IE62 ELISpot),具有潜在的转化应用前景,可为VZV的防治研究提供支持。 展开更多
关键词 水痘-带状疱疹病毒 感染性滴度检测方法 立即早期蛋白62 单克隆抗体 酶联免疫斑点技术
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辐流洁净室的建立与洁净度检测方法的研究 被引量:3
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作者 周玉岩 高燕霞 冯丽 《中国医院建筑与装备》 2015年第9期98-101,共4页
文章分析了辐流洁净室设计参数与建立的合理性,并探讨了在空态下辐流洁净室洁净度检测的具体参数与方法。
关键词 辐流洁净室 洁净度检测方法 粒子浓
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沥青路面平整度检测方法及病害原因分析
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作者 王峥 《住宅与房地产》 2019年第27期202-202,共1页
沥青路面的平整度病害会直接影响日常交通的安全性和舒适性,并增加对沥青路面的破坏。在使用期间,维护和修理压力机将使沥青表面软化。文章首先对沥青路面平整度检测方法进行了介绍,然后对沥青路面病害原因进行分析,最后提出了沥青路面... 沥青路面的平整度病害会直接影响日常交通的安全性和舒适性,并增加对沥青路面的破坏。在使用期间,维护和修理压力机将使沥青表面软化。文章首先对沥青路面平整度检测方法进行了介绍,然后对沥青路面病害原因进行分析,最后提出了沥青路面养护措施。 展开更多
关键词 沥青路面 平整度检测方法 病害原因 措施
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高等级公路路面平整度检测方法研究
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作者 张培培 《中文科技期刊数据库(全文版)工程技术》 2021年第4期51-51,53,共2页
作为一车一路系统的输入,高等级公路路面不平整的真实测定是对该系统进行研究的基础。因此,道路平整度检测一直是公路工程质量检验评估中的重要任务。如何实现快速,客观,准确,高效的道路平整度测试和指标,一直是公路建设单位,质量监督... 作为一车一路系统的输入,高等级公路路面不平整的真实测定是对该系统进行研究的基础。因此,道路平整度检测一直是公路工程质量检验评估中的重要任务。如何实现快速,客观,准确,高效的道路平整度测试和指标,一直是公路建设单位,质量监督验收部门,养护部门和科研院所关注的重点。为此,有必要弄清路面平整度的定义,并选择适当的检测方法和评估指标。 展开更多
关键词 高等级 公路路面 平整度检测方法
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基于车载多传感器的路面平整度检测方法 被引量:21
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作者 杜豫川 刘成龙 +1 位作者 吴荻非 蒋盛川 《中国公路学报》 EI CAS CSCD 北大核心 2015年第6期1-5,共5页
为提高路面平整度检测的便捷性,降低测量的能耗和成本,在国际平整度指数(IRI)计算模型的基础上,结合上海市多条沥青路面的测量实例,采用功率谱密度分析方法,提出了一种利用车内簧载竖向加速度推算IRI值的理论模型,开发了一套基于多个加... 为提高路面平整度检测的便捷性,降低测量的能耗和成本,在国际平整度指数(IRI)计算模型的基础上,结合上海市多条沥青路面的测量实例,采用功率谱密度分析方法,提出了一种利用车内簧载竖向加速度推算IRI值的理论模型,开发了一套基于多个加速度传感器和GPS的路面平整度检测原型系统,并采用控制变量法对比分析了加速度测量位点和车速对测量结果的影响。结果表明:IRI值与加速度功率谱密度均方根值具有线性关系;与单位点测量模型相比,双位点测量模型精度更高;测试车速对测量结果的影响可利用修正系数校正;该国际平整度检测系统具有能耗低、成本低、效率高等优势,适用于大范围的路面测量评估。 展开更多
关键词 道路工程 平整度检测方法 功率谱密分析 路面平整 加速传感器
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基于节点重要度的供应链级联失效检测方法 被引量:3
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作者 陈浩东 朱占峰 刘德智 《价格月刊》 北大核心 2022年第8期87-94,共8页
随着网络科学的发展,供应链网络中的级联失效现象对于供应链正常运作的巨大影响越来越受重视。针对这一现象,在节点重要度的基础上构建了系统化的级联失效检测方法,使用多指标评估模型,对供应链网络中节点进行了重要性分析,评价了供应... 随着网络科学的发展,供应链网络中的级联失效现象对于供应链正常运作的巨大影响越来越受重视。针对这一现象,在节点重要度的基础上构建了系统化的级联失效检测方法,使用多指标评估模型,对供应链网络中节点进行了重要性分析,评价了供应链级联最大连通子图规模变化以及供应链的破坏情况,通过仿真证明了方法的有效性并对模型参数的影响进行了分析。 展开更多
关键词 供应链网络 级联失效 节点 重要度检测方法
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论建筑施工中混凝土强度的检测方法 被引量:1
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作者 由丽华 《科技创新导报》 2013年第26期43-43,共1页
在当前的建筑工程施工中,混凝土是一种非常重要且常见的施工材料。由于混凝土只有在达到一定强度后才能充分体现出应用优势,因此确保混凝土的施工强度是建筑施工中的一项重要内容。现该文就来对建筑施工中混凝土的强度检测方法进行分析... 在当前的建筑工程施工中,混凝土是一种非常重要且常见的施工材料。由于混凝土只有在达到一定强度后才能充分体现出应用优势,因此确保混凝土的施工强度是建筑施工中的一项重要内容。现该文就来对建筑施工中混凝土的强度检测方法进行分析,探讨了在对无损混凝土与有损混凝土的不同要求下应当采取何种检测方法来确定混凝土的强度。 展开更多
关键词 建筑施工 混凝土 度检测方法
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Improved metrics for evaluating fault detection efficiency of test suite
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作者 王子元 陈林 +1 位作者 汪鹏 仉雪玲 《Journal of Southeast University(English Edition)》 EI CAS 2014年第3期285-288,共4页
By analyzing the average percent of faults detected (APFD) metric and its variant versions, which are widely utilized as metrics to evaluate the fault detection efficiency of the test suite, this paper points out so... By analyzing the average percent of faults detected (APFD) metric and its variant versions, which are widely utilized as metrics to evaluate the fault detection efficiency of the test suite, this paper points out some limitations of the APFD series metrics. These limitations include APFD series metrics having inaccurate physical explanations and being unable to precisely describe the process of fault detection. To avoid the limitations of existing metrics, this paper proposes two improved metrics for evaluating fault detection efficiency of a test suite, including relative-APFD and relative-APFDc. The proposed metrics refer to both the speed of fault detection and the constraint of the testing source. The case study shows that the two proposed metrics can provide much more precise descriptions of the fault detection process and the fault detection efficiency of the test suite. 展开更多
关键词 software testing test case prioritization fault detection efficiency METRIC
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Empirical Line Method Using Spectrally Stable Targets to Calibrate IKONOS Imagery
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作者 XU Jun-Feng 《Pedosphere》 SCIE CAS CSCD 2008年第1期124-130,共7页
By using speetrally stable targets, the empirical line (EL) method was tested to correct the multispectral IKONOS imagery acquired over Putuo Mountain, Zhejiang, China. A series of calibration targets, which were sp... By using speetrally stable targets, the empirical line (EL) method was tested to correct the multispectral IKONOS imagery acquired over Putuo Mountain, Zhejiang, China. A series of calibration targets, which were spectrally stable over time, were selected to establish the linear predicted equation. Subsequently, a series of spectrally stable validation targets were selected to assess the accuracy of the equations. And, validation targets, which were speetrally unstable over time, were used to test the feasibility of using the EL method to calibrate the archival remotely sensed data. Ground reflectance measurements for each target were made using an ASD FieldSpec spectroradiometer. A Trimble GeoXTTM GPS unit with sub-meter accuracy was used to estimate the target position accurately. Linear regression equations for four tKONOS bands were derived. The coefficients of determination for the blue, green, and red bands were all greater than 0.9800 and it was 0.9697 for the near infrared band. It was concluded that reasonable results could be obtained by using speetrally stable targets. 展开更多
关键词 CALIBRATION empirical line method IKONOS spectrally stable targets
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3D Surface Morphology Measurement and Auto-focusing System
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作者 CHEN Qi ZANG Huai-pei 《Semiconductor Photonics and Technology》 CAS 2005年第3期208-211,共4页
When interference microscope measures the surface rough of the micromechanical device, as soon as the work distance of interference microscope and the depth of field is shortened, the interference images become slur f... When interference microscope measures the surface rough of the micromechanical device, as soon as the work distance of interference microscope and the depth of field is shortened, the interference images become slur for the measured object if there has small interference after clear focus. The auto-focusing system is introduced into the interference microscope, the system can obtain high definition interference image rapidly,and can improve the measuring velocity and measuring precision. The system is characterized by auto-focusing range of ±150 μm, auto-focusing precision of ±0.3 μm, auto-focusing time of 4~8 s. 展开更多
关键词 3D surface morphology Interference microscope AUTO-FOCUSING
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Edge detection using directional eigenvalues of potential field gradient tensor data 被引量:2
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作者 ZHOU Shuai HUANG Danian 《Global Geology》 2015年第3期188-195,共8页
Edge detection of potential field interpretation is an important task. The traditional edge detection methods have poor ability in outlining weak amplitude anomalies clearly. The resolved edges position is blurred.We ... Edge detection of potential field interpretation is an important task. The traditional edge detection methods have poor ability in outlining weak amplitude anomalies clearly. The resolved edges position is blurred.We purposed new edge detection methods based on directional eigenvalues of potential field gradient tensor for the causative sources. In order to balance strong and weak amplitude anomalies simultaneously,we present one normalization method using different orders of vertical derivatives to improve the new filters. The presented filters were tested on synthetic and real potential field data to verify its feasibility. All of the results have shown that the new edge detection methods can not only display the sources edges precisely and clearly,but also bring out more geological subtle details. 展开更多
关键词 directional eigenvalues edge detection potential field
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Intrusion Detection Algorithm Based on Density,Cluster Centers,and Nearest Neighbors 被引量:6
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作者 Xiujuan Wang Chenxi Zhang Kangfeng Zheng 《China Communications》 SCIE CSCD 2016年第7期24-31,共8页
Intrusion detection aims to detect intrusion behavior and serves as a complement to firewalls.It can detect attack types of malicious network communications and computer usage that cannot be detected by idiomatic fire... Intrusion detection aims to detect intrusion behavior and serves as a complement to firewalls.It can detect attack types of malicious network communications and computer usage that cannot be detected by idiomatic firewalls.Many intrusion detection methods are processed through machine learning.Previous literature has shown that the performance of an intrusion detection method based on hybrid learning or integration approach is superior to that of single learning technology.However,almost no studies focus on how additional representative and concise features can be extracted to process effective intrusion detection among massive and complicated data.In this paper,a new hybrid learning method is proposed on the basis of features such as density,cluster centers,and nearest neighbors(DCNN).In this algorithm,data is represented by the local density of each sample point and the sum of distances from each sample point to cluster centers and to its nearest neighbor.k-NN classifier is adopted to classify the new feature vectors.Our experiment shows that DCNN,which combines K-means,clustering-based density,and k-NN classifier,is effective in intrusion detection. 展开更多
关键词 intrusion detection DCNN density cluster center nearest neighbor
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Experimental study on the compressive strength of grouted concrete block masonry based on nondestructive detection methods 被引量:1
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作者 姜洪斌 李龙飞 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2009年第2期189-193,共5页
Existing nondestructive detection methods were adopted to test the compressive strength of grouted concrete block masonry,i.e.the rebound method,pulling-out method and core drilling method were employed to test the st... Existing nondestructive detection methods were adopted to test the compressive strength of grouted concrete block masonry,i.e.the rebound method,pulling-out method and core drilling method were employed to test the strength of block,mortar and grouted concrete,respectively.The suitability of these methods for the testing of strength of grouted concrete block masonry was discussed,and the comprehensive strength of block masonry was appraised by combining existing nondestructive or micro-destructive detection methods.The nondestructive detection test on 25 grouted concrete block masonry specimens was carried out.Experimental results show that these methods mentioned above are applicable for the strength detection of grouted concrete block masonry.Moreover,the formulas of compressive strength,detection methods and proposals are given as well. 展开更多
关键词 grouted concrete block masonry compressive strength nondestructive detection
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Assessing proprioception:A critical review of methods 被引量:5
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作者 Jia Han Gordon Waddington +2 位作者 Roger Adams Judith Anson Yu Liuc 《Journal of Sport and Health Science》 SCIE 2016年第1期80-90,共11页
To control movement,the brain has to integrate proprioceptive information from a variety of mechanoreceptors.The role of proprioception in daily activities,exercise,and sports has been extensively investigated,using d... To control movement,the brain has to integrate proprioceptive information from a variety of mechanoreceptors.The role of proprioception in daily activities,exercise,and sports has been extensively investigated,using different techniques,yet the proprioceptive mechanisms underlying human movement control are still unclear.In the current work we have reviewed understanding of proprioception and the three testing methods:threshold to detection of passive motion,joint position reproduction,and active movement extent discrimination,all of which have been used for assessing proprioception.The origin of the methods,the different testing apparatus,and the procedures and protocols used in each approach are compared and discussed.Recommendations are made for choosing an appropriate technique when assessing proprioceptive mechanisms in different contexts. 展开更多
关键词 Joint motion sense Joint position sense Kinaesthesia Movement discrimination Propnoception
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The Study of Image Segmentation Based on the Combination of the Wavelet Multi-scale Edge Detection and the Entropy Iterative Threshold Selection 被引量:3
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作者 ZHANG Qian HE Jian-feng +3 位作者 MA Lei PAN Li-peng LIU Jun-qing CHEN Hong-lei 《Chinese Journal of Biomedical Engineering(English Edition)》 2013年第4期154-160,共7页
This paper proposes an image segmentation method based on the combination of the wavelet multi-scale edge detection and the entropy iterative threshold selection.Image for segmentation is divided into two parts by hig... This paper proposes an image segmentation method based on the combination of the wavelet multi-scale edge detection and the entropy iterative threshold selection.Image for segmentation is divided into two parts by high- and low-frequency.In the high-frequency part the wavelet multiscale was used for the edge detection,and the low-frequency part conducted on segmentation using the entropy iterative threshold selection method.Through the consideration of the image edge and region,a CT image of the thorax was chosen to test the proposed method for the segmentation of the lungs.Experimental results show that the method is efficient to segment the interesting region of an image compared with conventional methods. 展开更多
关键词 wavelet multi-scale ENTROPY iterative threshold lung images
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Compressive Strength Prediction of Porous Concrete Using Nondestructive Tests
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作者 Young Sang Cho Jeom Han Kim Sung Uk Hong 《Journal of Civil Engineering and Architecture》 2011年第12期1053-1064,共12页
This research focused on a prediction of compressive strength in porous concrete based on the ratio of air-entrained agents in the concrete slab using nondestructive testing methods such as the Impact Echo (IE) meth... This research focused on a prediction of compressive strength in porous concrete based on the ratio of air-entrained agents in the concrete slab using nondestructive testing methods such as the Impact Echo (IE) method, Spectral Analysis of Surface Wave (SASW) method and Free-Free Resonance (FFR) test. The method that best predicts the strength of the concrete slab can be derived from a relationship between compressive strengths and stress wave velocities. Concrete slab specimens of varying air content, were formed with a mix ratio of air-entrained agent of 0%, 0.15%, 0.3%, 0.7% and 1.5% by weight. These slabs were tested and analyzed to measure the stress wave velocities in order to develop a correlation with compressive strengths. The plot between the stress waves and compressive strengths showed a stiffslope up to an air ratio of 4% with a less steep slope beyond this point. In the process of predicting the compressive strength of concrete slab specimens, the prediction of compressive strength based on the compression wave velocity caused an average error of 4.9% in the compression wave velocity, and the prediction of compressive strength based on the surface wave velocity caused an average error of 2.2% in the surface wave velocity. 展开更多
关键词 Nondestructive test impact echo method SASW method free-free resonance test compressive strength air contents
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Analysis on the mechanism and characteristics of concrete strength testing methods
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作者 LI YanBing XIN Peng 《International English Education Research》 2016年第4期38-40,共3页
This paper mainly described five kinds of common detection methods, including the test block method, core drilling method, rebound method, dialing out method and ultrasonic testing method and systematically analyzed t... This paper mainly described five kinds of common detection methods, including the test block method, core drilling method, rebound method, dialing out method and ultrasonic testing method and systematically analyzed the mechanism of all kinds of concrete strength detection methods as well as their advantages and disadvantages, which would provide a guide for construction personnel and research institutions to choose an economical ,accurate and safe detection method. 展开更多
关键词 concrete strength inspection mechanism CHARACTERISTICS
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