Genes encoding enzymes involved in biosynthesis of very long chain fatty acids were significantly up-regulatedduring early cotton fiber development. Two cDNAs, GhKCR1 and GhKCR2 encoding putative cotton 3-ketoacyl-CoA...Genes encoding enzymes involved in biosynthesis of very long chain fatty acids were significantly up-regulatedduring early cotton fiber development. Two cDNAs, GhKCR1 and GhKCR2 encoding putative cotton 3-ketoacyl-CoAreductases that catalyze the second step in fatty acid elongation, were isolated from developing cotton fibers. GhKCR1and 2 contain open reading frames of 963 bp and 924 bp encoding proteins of 320 and 307 amino acid residues,respectively. Quantatitive RT-PCR analysis showed that both these genes were highly preferentially expressed duringthe cotton fiber elongation period with much lower levels recovered from roots, stems and leaves. GhKCR1 and 2showed 30%-32% identity to Saccharomyces cerevisiae Ybr159p at the deduced amino acid level. These cotton cDNAswere cloned and expressed in yeast haploid ybr159w? mutant that was deficient in 3-ketoacyl-CoA reductase activity.Wild-type growth rate was restored in ybr159w? cells that expressed either GhKCR1 or 2. Further analysis showed thatGhKCR1 and 2 were co-sedimented within the membranous pellet fraction after high-speed centrifugation, similar to theyeast endoplasmic reticulum marker ScKar2p. Both GhKCR(s) showed NADPH-dependent 3-ketoacyl-CoA reductaseactivity in an in vitro assay system using palmitoyl-CoA and malonyl-CoA as substrates. Our results suggest thatGhKCR1 and 2 are functional orthologues of ScYbr159p.展开更多
With the rapid development of network technology, it is increasingly important to dynamically upgrade router’s software. In this paper, the authors present new software architecture to be applied to extensible servic...With the rapid development of network technology, it is increasingly important to dynamically upgrade router’s software. In this paper, the authors present new software architecture to be applied to extensible services router. The novel feature of the architecture is the ability to dynamically load and configure extensible components at run time. Each component implements one function such as packets classification, packets scheduling and routing protocol. We design a simple configuration language (ESRCL) to configure and manage the router. The architecture can be configured according to the packet flows. At present, a prototype system based on highly efficient router operating system (HEROS) has been finished.展开更多
Based on the framework of the extended finite element method (XFEM), the enriched exponent discontinuous function is modified properly by introducing the rigidity ratio of two sides materials of interface crack, and t...Based on the framework of the extended finite element method (XFEM), the enriched exponent discontinuous function is modified properly by introducing the rigidity ratio of two sides materials of interface crack, and the portion integral scheme is adopted for interface elements containing two materials. To embody the singularity of the crack tip, the triangle function is introduced directly. What’s more, the maximum loop stress fracture criterion is adopted to determine the extension direction in extended material domains, and the true extension distance for each load step is determined by reducing or increasing half the current trial extension distance until the equivalent stress intensity factor reaches the type I fracture toughness of material. Finally, with the improved XFEM, the interface crack propagation in a cantilever deep beam and concrete gravity dam are simulated without re-meshing respectively and their failure modes are also analyzed.展开更多
Infinitesimal prolongation theorem is extended from sequences to nets based on κ-saturated nonstandard model. As its an application, a main property about topology of uniform convergence is proved. The proof is much ...Infinitesimal prolongation theorem is extended from sequences to nets based on κ-saturated nonstandard model. As its an application, a main property about topology of uniform convergence is proved. The proof is much simpler than it was, meanwhile the nonstandard characteristics of convergence with respect to u.c. topology is given.展开更多
基金supported by grants from China Na-tional Basic Research Program (NO. 2004CB117302)National Natural Science Foundation of China (No.30470171)the Sigrid Jusélius Foundation Finland and the Academy of Finland
文摘Genes encoding enzymes involved in biosynthesis of very long chain fatty acids were significantly up-regulatedduring early cotton fiber development. Two cDNAs, GhKCR1 and GhKCR2 encoding putative cotton 3-ketoacyl-CoAreductases that catalyze the second step in fatty acid elongation, were isolated from developing cotton fibers. GhKCR1and 2 contain open reading frames of 963 bp and 924 bp encoding proteins of 320 and 307 amino acid residues,respectively. Quantatitive RT-PCR analysis showed that both these genes were highly preferentially expressed duringthe cotton fiber elongation period with much lower levels recovered from roots, stems and leaves. GhKCR1 and 2showed 30%-32% identity to Saccharomyces cerevisiae Ybr159p at the deduced amino acid level. These cotton cDNAswere cloned and expressed in yeast haploid ybr159w? mutant that was deficient in 3-ketoacyl-CoA reductase activity.Wild-type growth rate was restored in ybr159w? cells that expressed either GhKCR1 or 2. Further analysis showed thatGhKCR1 and 2 were co-sedimented within the membranous pellet fraction after high-speed centrifugation, similar to theyeast endoplasmic reticulum marker ScKar2p. Both GhKCR(s) showed NADPH-dependent 3-ketoacyl-CoA reductaseactivity in an in vitro assay system using palmitoyl-CoA and malonyl-CoA as substrates. Our results suggest thatGhKCR1 and 2 are functional orthologues of ScYbr159p.
文摘With the rapid development of network technology, it is increasingly important to dynamically upgrade router’s software. In this paper, the authors present new software architecture to be applied to extensible services router. The novel feature of the architecture is the ability to dynamically load and configure extensible components at run time. Each component implements one function such as packets classification, packets scheduling and routing protocol. We design a simple configuration language (ESRCL) to configure and manage the router. The architecture can be configured according to the packet flows. At present, a prototype system based on highly efficient router operating system (HEROS) has been finished.
基金supported by the National Natural Science Foundation of China (Grant No. 10972072)the National Basic Research Program of China ("973" Project) (Grant No. 2007CB714104)the Special Fund of State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering at Hohai University (Grant No. 2009585912)
文摘Based on the framework of the extended finite element method (XFEM), the enriched exponent discontinuous function is modified properly by introducing the rigidity ratio of two sides materials of interface crack, and the portion integral scheme is adopted for interface elements containing two materials. To embody the singularity of the crack tip, the triangle function is introduced directly. What’s more, the maximum loop stress fracture criterion is adopted to determine the extension direction in extended material domains, and the true extension distance for each load step is determined by reducing or increasing half the current trial extension distance until the equivalent stress intensity factor reaches the type I fracture toughness of material. Finally, with the improved XFEM, the interface crack propagation in a cantilever deep beam and concrete gravity dam are simulated without re-meshing respectively and their failure modes are also analyzed.
基金Supported by the Speaial Science Foundation of the Edneational Committee of Shaanxi Province(oojk207).
文摘Infinitesimal prolongation theorem is extended from sequences to nets based on κ-saturated nonstandard model. As its an application, a main property about topology of uniform convergence is proved. The proof is much simpler than it was, meanwhile the nonstandard characteristics of convergence with respect to u.c. topology is given.
