Inbred of Wu Zhishan miniature pig(WZSP)at 5 to 8 Mouths of age,which were used as embryo donors.The total 75 fetuses was collected from 14 donors at day 25 to 29 of pregnancy(estrus=day 0),which was used for in vitro...Inbred of Wu Zhishan miniature pig(WZSP)at 5 to 8 Mouths of age,which were used as embryo donors.The total 75 fetuses was collected from 14 donors at day 25 to 29 of pregnancy(estrus=day 0),which was used for in vitro culture,clone,isolation,passage of porcine embryonic germ(PEG).The results were shown that:(1)The PGCs of fetuses between days 2729 of pregnancy(estrus=0)was easily to collect and more suitable to set up PEGs.(2)The PEGs clones with WZSP was coming out 2 days early than Shim(97),Piedrahita(98)and Mueller(99)reported previously.(3)PEG′s will be survival in vitro after thawing.展开更多
文摘Inbred of Wu Zhishan miniature pig(WZSP)at 5 to 8 Mouths of age,which were used as embryo donors.The total 75 fetuses was collected from 14 donors at day 25 to 29 of pregnancy(estrus=day 0),which was used for in vitro culture,clone,isolation,passage of porcine embryonic germ(PEG).The results were shown that:(1)The PGCs of fetuses between days 2729 of pregnancy(estrus=0)was easily to collect and more suitable to set up PEGs.(2)The PEGs clones with WZSP was coming out 2 days early than Shim(97),Piedrahita(98)and Mueller(99)reported previously.(3)PEG′s will be survival in vitro after thawing.
