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Knockout血清替代品可提高C57BL/6J小鼠胚胎干细胞建系效率 被引量:8
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作者 王宏 田海滨 +3 位作者 陈娟 沙红英 陈建泉 成国祥 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2007年第3期269-272,共4页
目的:在培养液中添加knockout血清替代品(knockout serum replacement,KSR)代替胎牛血清(FBS)用于建立C57BL/6J小鼠胚胎干细胞(ESC)细胞系,以便消除血清中的不确定因子对ESC增殖的影响。方法:以C57BL/6J小鼠3.5 d的囊胚为材料分离ESC,比... 目的:在培养液中添加knockout血清替代品(knockout serum replacement,KSR)代替胎牛血清(FBS)用于建立C57BL/6J小鼠胚胎干细胞(ESC)细胞系,以便消除血清中的不确定因子对ESC增殖的影响。方法:以C57BL/6J小鼠3.5 d的囊胚为材料分离ESC,比较KSR和FBS用于建立小鼠ESC细胞系的效率,并通过体内、外分化验证所分离获得的小鼠ESC的发育潜能。结果:培养液中添加KSR,成功从13个小鼠囊胚中分离获得一个ESC细胞系(MES-1),体外培养传代超过20代仍保持未分化状态,核型为正常XX型,碱性磷酸酶及oct-4基因高表达,悬浮培养可以生成拟胚体,接种到裸鼠皮下可形成畸胎瘤,注射到ICR小鼠3.5 d囊胚中,ESC可以参与胚胎发育并产生嵌合体小鼠。而培养液中添加FBS的对照组未能获得超过3代的ESC细胞系。结论:在培养液中添加KSR代替FBS适合于C57BL/6J小鼠ESC的分离与培养,从而可避免实验前对所用血清的筛选。 展开更多
关键词 knockout血清替代品 C57BL/6J小鼠 胚胎干细胞 建系效率
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Study on How to Design Knitted Fabric Pattern with Mathematical Theory 被引量:2
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作者 陈莉 张蕊 叶介茂 《Journal of Donghua University(English Edition)》 EI CAS 2009年第2期204-206,共3页
To improve design capabilities for the knitted fabric pattern,a preliminary study is carried out for the design method.Based on mathematical theory,pattern could be created automatically by computer with inputting dif... To improve design capabilities for the knitted fabric pattern,a preliminary study is carried out for the design method.Based on mathematical theory,pattern could be created automatically by computer with inputting different parameters for mathematical functions.The knitted fabric simulation is realized by M1 CAD pattern preparation system of STOLL Company.In the pattern,different color unit is replaced by obverse stitch with different color,or by obverse stitch and reverse stitch with the same color separately,after that the effects of knitted fabrics could be simulated.Designing with this method,it would not only help to acquire a great of diversity patterns,but also improve design efficiency and save cost. 展开更多
关键词 knitted fabric mathematical theory patterndesign CAD fabric simulation
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Convenient and inexpensive determination of optical constants and film thickness of blended organic thin film
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作者 LIANG QiYing CHEN Jie +3 位作者 LI Xin GAO ZhiQiang MI BaoXiu YANG ZhenHua 《Science China(Physics,Mechanics & Astronomy)》 SCIE EI CAS CSCD 2015年第2期26-32,共7页
This work presents the study of optical constants and film thickness of blended organic thin films, emphasizing on the modeling procedure with modified genetic algorithm aided by absorption or transmittance spectra of... This work presents the study of optical constants and film thickness of blended organic thin films, emphasizing on the modeling procedure with modified genetic algorithm aided by absorption or transmittance spectra of both pure materials and the blends. Taking the blending of copper phthalocyanine(Cu Pc) and fullerene(C60) as an example, a simple, convenient and low-cost method for the determination of the optical constants and film thickness of blended organic thin films was demonstrated. New scheme for optical modeling of blended organic thin film was proposed by introducing peak energies of Cody-Lorentz oscillators of the pure materials, which were determined by fitting the film absorption of pure materials. These oscillators of pure materials could be recognized in the transmittance spectrum of their blends, and were further used as the initial searching ranges in the simulation of blended films. As a result, the constraint bounds of the unknown parameters were significantly reduced and modeling efficiency as well as fitting accuracy was improved. For instance, the fitting of the transmittance curves of blended films with different blending ratios reached reliable results in comparison with extinction coefficients obtained from experiment. 展开更多
关键词 organic photovoltaic optical modelling blended thin film CuPc: C60 optical constant
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