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利用SRAP分子标记分析47份杂草稻样品遗传多样性 被引量:11
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作者 刘丹 孙健 +4 位作者 马殿荣 王嘉宇 唐亮 苗微 陈温福 《中国水稻科学》 CAS CSCD 北大核心 2012年第1期70-76,共7页
利用SRAP分子标记分析了采集于杂草稻多发地区的47份样本的遗传多样性,旨在从基因的开放式阅读框区域(ORFs)深入研究杂草稻的遗传背景,为进一步揭示其来源提供依据。结果表明,参试的我国杂草稻材料在110对引物组合中共扩增出845种类型... 利用SRAP分子标记分析了采集于杂草稻多发地区的47份样本的遗传多样性,旨在从基因的开放式阅读框区域(ORFs)深入研究杂草稻的遗传背景,为进一步揭示其来源提供依据。结果表明,参试的我国杂草稻材料在110对引物组合中共扩增出845种类型的谱带,多态性谱带590种,多态率为69.82%,平均每对引物可以检测到5.4条多态性谱带。说明SRAP分子标记对于杂草稻基因组开放式阅读框区域序列的多态性具有很高的检测效率,适用于遗传多样性研究。我国杂草稻材料Shannon-Weaver多样性指数(I)为0.63,且南北方杂草稻多样性指数(I)相当,分别为0.63和0.62。聚类分析结果表明,南方收集的杂草稻与北方收集杂草稻群体间遗传分化较大,遗传差异明显。程氏指数判定结果也表明杂草稻呈南籼北粳的分布状态。 展开更多
关键词 杂草稻 开放式阅读框 相关序列扩增多态性 遗传多样性
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Expression and localization of a novel phosducin-like protein from amphioxus Branchiostoma belcheri
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作者 萨仁高娃 赵永刚 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2009年第2期324-330,共7页
A full length amphioxus cDNA, encoding a novel phosducin-like protein (Amphi-PhLP), was identified for the first time from the gut cDNA library of Branchiostoma belcheri. It is comprised of 1 550 bp and an open read... A full length amphioxus cDNA, encoding a novel phosducin-like protein (Amphi-PhLP), was identified for the first time from the gut cDNA library of Branchiostoma belcheri. It is comprised of 1 550 bp and an open reading frame (ORF) of 241 amino acids, with a predicted molecular mass of approximately 28 kDa. In situ hybridization histochemistry revealed a tissue-specific expression pattern of Amphi-PhLP with the high levels in the ovary, and at a lower level in the hind grit and testis, hepatic caecum, gill, endostyle, and epipbaryngeal groove, while it was absent in the muscle, neural tube and notochord. In the Chinese Hamster Ovary (CHO) cells transfected with the expression plasmid pEGFP-NI/Amphi-PhLP, the fusion protein was targeted in the cytoplasm of CHO cells, suggesting that Amphi-PhLP is a cytosolic protein. This work may provide a framework for further understanding of the physiological function of Amphi-PhLP in B. belcheri. 展开更多
关键词 amphioxus B. belcheri Amphi-PhLP northern blotting in situ hybridization cellularlocalization
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