[Objective]The aim was to select the suitable hormone factors for flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.[Method]The test-tube plantlets from the stems of Dendrobium officinate Ki...[Objective]The aim was to select the suitable hormone factors for flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.[Method]The test-tube plantlets from the stems of Dendrobium officinate Kimura et Migo were used as the experimental materials and MS medium as the basic medium.Comparative tests have been done between single-factor hormone treatments(different concentrations of PP333 or TDZ) and multi-factor hormone treatments(different combinations of PP333,TDZ,6-BA and NAA) to research the effects of hormone factors on the flowering induction of the plantlets.[Result]Among the single-factor hormone treatments,the suitable concentration and the rate of flower buds formation of PP333 treatment were 0.2 mg/L and 8.5%,the that of TDZ treatment were 0.06 mg/L and 15.5%;the effects of multi-factor hormone treatments on the flowering induction were ordered as follow:(PP333 + 6-BA + NAA + TDZ)〉 (PP333 + 6-BA + NAA)〉 (PP333 + 6-BA) and(PP333 + NAA) ;the most suitable treatment was PP333 0.3 mg/L + 6-BA 0.5 mg/L + NAA 0.5 mg/L + TDZ 0.06 mg/L,the rate of flower bud formation and the rate of the blossomed flower were respectly reached to 80.4% and 90.3%.[Conclusion]PP333 and TDZ showed the important effect on the flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.The effect of TDZ was better than that of PP333.It is much more conducive to the flower bud formation,when using appropriate concentration of TDZ combined with other hormones properly.展开更多
By determination of the change of endogenous hormone Zr,iPA,GA3,IAA and ABA during different flower bud differentiation stages of Phyllostachys praecox,which is identified through both field observation and lab analys...By determination of the change of endogenous hormone Zr,iPA,GA3,IAA and ABA during different flower bud differentiation stages of Phyllostachys praecox,which is identified through both field observation and lab analysis,and with the reference to the previous research achievements on bamboo flowering,the flowering mechanism assumption of Phyto-Hormone Regulation and Gene Activation of Ph.praecox is induced in this article:Bamboo flower bud differentiation can be divided into 3 stages,i.e.flower bud induction,flower bud initiation and flower bud development;Bamboo leaves sense and receive flowering signals from environments to change its hormone level,esp.ratios of iPA/ABA and iPA/GA3;Flowering gene is activated once the ratios of iPA/ABA and iPA/GA3 reach a proper threshold,and it produces DNA and RNA carrying flowering code and transports them to top or side buds nearby,and then protein necessary for flower bud differentiation comes out,as a result of which the flower bud induction is trigged and started,followed by flower bud initiation and development.In the induction stage,ratio of C/N is nearly constant,but increases in the initiation stage.Therefore it clarifies that the rising of C/N ratio does not bring about bamboo flowering initially,and it is a follow-up reactions of process initiation of bamboo flowering.It proves that bamboo rhizome is directly involved in the flower bud differentiation.This assumption can well explain mysterious phenomena of bamboo flowering,and by integrating the current several assumptions,answer the difficult and perplexing questions regarding bamboo flowering which have not been answered by the present assumptions.展开更多
基金Supported by Plan Project of Science and Technology Committee of Guangxi Province (0322024-3A) Scientific Research Foundation for Returned Scholars of Guangxi Province~~
文摘[Objective]The aim was to select the suitable hormone factors for flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.[Method]The test-tube plantlets from the stems of Dendrobium officinate Kimura et Migo were used as the experimental materials and MS medium as the basic medium.Comparative tests have been done between single-factor hormone treatments(different concentrations of PP333 or TDZ) and multi-factor hormone treatments(different combinations of PP333,TDZ,6-BA and NAA) to research the effects of hormone factors on the flowering induction of the plantlets.[Result]Among the single-factor hormone treatments,the suitable concentration and the rate of flower buds formation of PP333 treatment were 0.2 mg/L and 8.5%,the that of TDZ treatment were 0.06 mg/L and 15.5%;the effects of multi-factor hormone treatments on the flowering induction were ordered as follow:(PP333 + 6-BA + NAA + TDZ)〉 (PP333 + 6-BA + NAA)〉 (PP333 + 6-BA) and(PP333 + NAA) ;the most suitable treatment was PP333 0.3 mg/L + 6-BA 0.5 mg/L + NAA 0.5 mg/L + TDZ 0.06 mg/L,the rate of flower bud formation and the rate of the blossomed flower were respectly reached to 80.4% and 90.3%.[Conclusion]PP333 and TDZ showed the important effect on the flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.The effect of TDZ was better than that of PP333.It is much more conducive to the flower bud formation,when using appropriate concentration of TDZ combined with other hormones properly.
文摘By determination of the change of endogenous hormone Zr,iPA,GA3,IAA and ABA during different flower bud differentiation stages of Phyllostachys praecox,which is identified through both field observation and lab analysis,and with the reference to the previous research achievements on bamboo flowering,the flowering mechanism assumption of Phyto-Hormone Regulation and Gene Activation of Ph.praecox is induced in this article:Bamboo flower bud differentiation can be divided into 3 stages,i.e.flower bud induction,flower bud initiation and flower bud development;Bamboo leaves sense and receive flowering signals from environments to change its hormone level,esp.ratios of iPA/ABA and iPA/GA3;Flowering gene is activated once the ratios of iPA/ABA and iPA/GA3 reach a proper threshold,and it produces DNA and RNA carrying flowering code and transports them to top or side buds nearby,and then protein necessary for flower bud differentiation comes out,as a result of which the flower bud induction is trigged and started,followed by flower bud initiation and development.In the induction stage,ratio of C/N is nearly constant,but increases in the initiation stage.Therefore it clarifies that the rising of C/N ratio does not bring about bamboo flowering initially,and it is a follow-up reactions of process initiation of bamboo flowering.It proves that bamboo rhizome is directly involved in the flower bud differentiation.This assumption can well explain mysterious phenomena of bamboo flowering,and by integrating the current several assumptions,answer the difficult and perplexing questions regarding bamboo flowering which have not been answered by the present assumptions.