Mitochondrial 12S and 16S ribosomal RNA genes sequences were sequenced using dye-labeled terminator on an ABI 377 automated sequencer in 11 individuals and 1 species' sequences were gained from GenBank,representin...Mitochondrial 12S and 16S ribosomal RNA genes sequences were sequenced using dye-labeled terminator on an ABI 377 automated sequencer in 11 individuals and 1 species' sequences were gained from GenBank,representing 6 genera of family Tetrigidae.The collated sequences were aligned using Clustal X version 1.81 and then,the sequence variability and heredity distances based on Kimura 2-parameter model were calculated using Mega 2.1.In obtained sequences (736 bp),the average A+T content is 73.9%,ranging from 71.2% to 77.5%;the overall G+C content is 26.1%,ranging from 22.5% to 28.8%.Based on alignment of the combined sequences,185 parsimony-informative sites were revealed in 755 available base pairs.Phylogenetic trees were reconstructed using NJ,MP and ML methods with Cylindraustralia kochii as outgroup.The results indicated that the monophyletic nature of Tetrix is questioned and suggest that T.tubercarina may be given tribal rank.Our results also show that Coptltettix huanjiangensis and C.gongshanensis are the same species,i.e.Coptltettix gongshanensis Zheng,and C.huanjiangensis is the synonyms of C.gongshanensis.展开更多
In Internet of Things(IoT), the devices or terminals are connected with each other, which can be very diverse over the wireless networks. Unfortunately, the current devices are not designed to communicate with the col...In Internet of Things(IoT), the devices or terminals are connected with each other, which can be very diverse over the wireless networks. Unfortunately, the current devices are not designed to communicate with the collocated devices which employ different communication technologies. Consequently, the communication between these devices will be realized only by using the gateway nodes. This will cause the inefficient use of wireless resources. Therefore, in this paper, a smart service system(SSS) architecture is proposed, which consists of smart service terminal(SST), and smart service network(SSN), to realize the Io T in a general environment with diverse communication networks, devices, and services. The proposed architecture has the following advantages: i) the devices in this architecture cover multiple types of terminals and sensor-actuator devices; ii) the communications network therein is a converged network, and will coordinate multiple kinds of existing and emerging networks. This converged network offers ubiquitous access for various sensors and terminals; iii) the architecture has services and applications covering all smart service areas. It also provides theadaptability to new services and applications. A SSS architecture-based smart campus system was developed and deployed. Evaluation experiments of the proposed smart campus system demonstrate the SSS's advantages over the existing counterparts, and verify the effectiveness of the proposed architecture.展开更多
文摘Mitochondrial 12S and 16S ribosomal RNA genes sequences were sequenced using dye-labeled terminator on an ABI 377 automated sequencer in 11 individuals and 1 species' sequences were gained from GenBank,representing 6 genera of family Tetrigidae.The collated sequences were aligned using Clustal X version 1.81 and then,the sequence variability and heredity distances based on Kimura 2-parameter model were calculated using Mega 2.1.In obtained sequences (736 bp),the average A+T content is 73.9%,ranging from 71.2% to 77.5%;the overall G+C content is 26.1%,ranging from 22.5% to 28.8%.Based on alignment of the combined sequences,185 parsimony-informative sites were revealed in 755 available base pairs.Phylogenetic trees were reconstructed using NJ,MP and ML methods with Cylindraustralia kochii as outgroup.The results indicated that the monophyletic nature of Tetrix is questioned and suggest that T.tubercarina may be given tribal rank.Our results also show that Coptltettix huanjiangensis and C.gongshanensis are the same species,i.e.Coptltettix gongshanensis Zheng,and C.huanjiangensis is the synonyms of C.gongshanensis.
基金supported by the national 973 project of China under Grants 2013CB329104the Natural Science Foundation of China under Grants 61372124, 61427801+1 种基金the Natural Science Foundation of the Jiangsu Higher Education Institutions (Grant No.13KJB520029)the Jiangsu Province colleges and universities graduate students scientific research and innovation program CXZZ13_0477,NUPTSF(Grant No.NY214033)
文摘In Internet of Things(IoT), the devices or terminals are connected with each other, which can be very diverse over the wireless networks. Unfortunately, the current devices are not designed to communicate with the collocated devices which employ different communication technologies. Consequently, the communication between these devices will be realized only by using the gateway nodes. This will cause the inefficient use of wireless resources. Therefore, in this paper, a smart service system(SSS) architecture is proposed, which consists of smart service terminal(SST), and smart service network(SSN), to realize the Io T in a general environment with diverse communication networks, devices, and services. The proposed architecture has the following advantages: i) the devices in this architecture cover multiple types of terminals and sensor-actuator devices; ii) the communications network therein is a converged network, and will coordinate multiple kinds of existing and emerging networks. This converged network offers ubiquitous access for various sensors and terminals; iii) the architecture has services and applications covering all smart service areas. It also provides theadaptability to new services and applications. A SSS architecture-based smart campus system was developed and deployed. Evaluation experiments of the proposed smart campus system demonstrate the SSS's advantages over the existing counterparts, and verify the effectiveness of the proposed architecture.