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清肿瘤性异基因造血干细胞移植 被引量:2
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作者 达万明 冯四洲 《生物医学工程与临床》 CAS 2012年第1期94-98,共5页
标准的清髓性异基因造血干细胞移植(allo-HSCT)对于需代替治疗的造血与免疫系统的非恶性疾病,应当是合理或足够的;然而,对于恶性血液病患者,清除患者骨髓造血组织,成功重建异体正常造血与免疫系统,并不一定能完全治愈恶性血液病,因为白... 标准的清髓性异基因造血干细胞移植(allo-HSCT)对于需代替治疗的造血与免疫系统的非恶性疾病,应当是合理或足够的;然而,对于恶性血液病患者,清除患者骨髓造血组织,成功重建异体正常造血与免疫系统,并不一定能完全治愈恶性血液病,因为白血病(干)细胞并非只限骨髓中存在,它可浸润骨髓之外的其他任何组织。临床实践证实,allo-HSCT后仍然有30%左右的患者疾病复发,特别是具有高危因素或难治复发患者复发率可高达40%~70%以上。这些复发的白血病细胞几乎全系源自患者移植前本身的白血病细胞,其中半数患者以髓外部位复发开始,有证据提示,清髓性移植并没有完全杀灭患者体内的白血病细胞,特别是那些对化放疗不敏感或栖居在髓外"庇护所"中的白血病干细胞,最终导致疾病复发。因此笔者提出并建立了一个清肿瘤性异体造血干细胞移植(TAHSCT)的概念,在临床上对其进行了初步的探讨。其内容贯穿于移植技术全过程的各个环节,但主要为应用个体化清肿瘤性预处理方案和加强移植后免疫治疗。 展开更多
关键词 清髓性异基因造血干细胞 干细胞移植 肿瘤异体造血干细胞移植 血液病
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癌症生物标记物和个体化医疗 被引量:1
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作者 窦雪琳 白春梅 《中国医学科学院学报》 CAS CSCD 北大核心 2015年第1期113-117,共5页
癌症生物标记物的发展引领癌症的诊治从最早基于肿瘤类型进行治疗,已过度到基于分子靶点开展治疗的新纪元,后者也标志着个体化治疗的开端。每一个癌症个体的分子信息都是独特的,真正的个体化治疗应该着重于个体的分子信息特征,用以指导... 癌症生物标记物的发展引领癌症的诊治从最早基于肿瘤类型进行治疗,已过度到基于分子靶点开展治疗的新纪元,后者也标志着个体化治疗的开端。每一个癌症个体的分子信息都是独特的,真正的个体化治疗应该着重于个体的分子信息特征,用以指导临床决策。 展开更多
关键词 个体化医疗 癌症生物标记物 非小细胞肺癌 异体肿瘤移植
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The Anti—tumor Effects of an Anti—CD71 Chimeric Antibody in Vitro and Its Distribution in a Tumor Xenograft Model 被引量:2
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作者 YANGDaofeng WANGShuo 《The Chinese-German Journal of Clinical Oncology》 CAS 2002年第2期109-112,共4页
Objective To investigate the anti-tumor effects in vitro and in vivo distribution of the human/murine chimeric antibody (D2C). Methods The CD71 positive target cells (K562, GEM and SMMC7721) and the effector cells, fr... Objective To investigate the anti-tumor effects in vitro and in vivo distribution of the human/murine chimeric antibody (D2C). Methods The CD71 positive target cells (K562, GEM and SMMC7721) and the effector cells, freshly isolated human PBMC, with the ratio of target cells to effector cells 1:50, were incubated in various dilutions of D2C antibody ( Ab) . Antibody dependent cytotoxicity (AD-CC) was tested by using an LDH-release assay. Instead of effector cells, complement was added to the target cells (GEM, SMMC-7721) with various dilutions of D2C Ab. A method of counting death cells was used in complement dependent cytotoxicity (CDC) assay. Tumor localization and distribution of the chimeric antibody (D2C) were observed by labeling the chimeric Ab with radioiodine(131I) and injecting it into nude mice (Balb/c nu/nu) transplanted with human hepatocellular carcinoma cells (SMMC-7721).Results A significant ADCC was observed with the increased concentration of the D2C Ab. Cytolysis of CD71-positive target cells by the D2C Ab was found in the presence of fresh rabbit complement. Labeled D2C administered by intraperitoneal as well as tumor regional injection, was visualized by SPECT. The distribution of D2C Ab in murine organs and tissues showed that non-specific binding was lower following tumor regional administration than when the antibody was administered by an intraperitoneal injection. The human/murine chimeric antibody (D2C) has in vitro anti-tumor effects and can exert its effects in specific tumor localization. Its distribution and local effects in vivo can be detected by radioimmunoimaging.Conclusion CD71 human/murine chimeric antibody showed marked killing of tumor cells in vitro, and specific recognition and high affinity binding to tumor tissue in vivo 展开更多
关键词 CD71 human/murine chimeric antibody ADCC CDC
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Massive allograft replacement in management of bone tumors 被引量:2
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作者 Xiaohui Niu Lin Hao Qing Zhang Yi Ding 《The Chinese-German Journal of Clinical Oncology》 CAS 2008年第3期159-163,共5页
Objective: To evaluate the functional outcome and complications of allograft replacement in management of bone tumors. Methods: Between March 1992 and September 2002, 164 patients underwent bone tumor resection and ... Objective: To evaluate the functional outcome and complications of allograft replacement in management of bone tumors. Methods: Between March 1992 and September 2002, 164 patients underwent bone tumor resection and massive allograft reconstruction of bone defects. The length of the resected part ranged from 5-35 cm. The resections were classified as marginal or wide resections of the tumor on the basis of the Musculoskeletal Tumor Society staging system. Fresh-frozen allografts were employed as osteoarticular grafts (n = 95), hemi-condylar (n = 15), massive (n = 23), allograft-prosthesis composite (n = 12), intercalary grafts (n = 15) or hemi-pelvic grafts (n = 4). Most of the lesions were osteosarcoma and giant cell tumor of bone and located in proximal and distal femur, proximal tibia and humerus. Results: At a median follow-up of 47 months (range, 12 to 168 months) after the operation, 154 of the patients in the study were free of disease and 10 died of disease. Twenty-one (12.8%) patients had local recurrence and 38 (23.2%) nonunion. Late complications included 11 (6.7%) fractures of the allograft and 18 (11.0%) infections of the graft, instability of the joint in the form of subluxation was noted in 13 (7.9%) patients. Ten extremities were amputated due to local recurrence or severe infection. Conclusion: AIIografts can be used for reconstruction of bony defects after tumor resection. AIIograft has nearly similar shape, strength, osteo-inductivity and osteo-conductivity with host bone. AIIograft implantation is a high complication reconstruction method, and the dsk of recurrence increases when less surgical margin achieves. 展开更多
关键词 bone neoplasms bone transplantation ALLOGRAFTS
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原发性乳腺原位癌和浸润性癌裸鼠移植 被引量:1
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作者 贺兰湘 Johan A.Andersen 《中华肿瘤杂志》 CAS CSCD 北大核心 1996年第5期321-323,共3页
为了建立早期和晚期原发性乳腺癌的裸鼠模型,将15例乳腺原位癌和31例浸润性癌移植于155只裸鼠皮下。新鲜的癌组织被制备成细胞粗悬液和组织小块,分别植入到裸鼠两背侧皮下,同时植入一粒17-β-雌二醇缓释丸于右侧皮下。在... 为了建立早期和晚期原发性乳腺癌的裸鼠模型,将15例乳腺原位癌和31例浸润性癌移植于155只裸鼠皮下。新鲜的癌组织被制备成细胞粗悬液和组织小块,分别植入到裸鼠两背侧皮下,同时植入一粒17-β-雌二醇缓释丸于右侧皮下。在10个月观察期中,原位癌终未长出,光镜下可见移植物仍维持原位癌的结构特征,未见浸润性发展。31例浸润性癌中仅1例明显生长并被反复传代。该原发瘤的病理诊断是多中心去分化导管癌,在裸鼠体内生长的移植瘤经鉴定不表达雌、孕激素受体,p53蛋白阴性,cerbB-2蛋白阳性,DNA倍性为近四倍体。经多次传代后,移植瘤仍能维持原发瘤的各种特征,因此这一模型的建立为晚期乳癌的研究提供了一个新的工具。早期乳癌的裸鼠模型虽未建成。 展开更多
关键词 乳腺肿瘤 原位癌 浸润性导管癌 肿瘤异体移植
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索拉非尼通过调控Runt相关转录因子3-血管内皮生长因子通路抑制肝癌血管生成 被引量:3
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作者 柴梦音 寇卜心 +4 位作者 伏志 魏飞力 豆双双 陈德喜 刘晓霓 《中华肝脏病杂志》 CAS CSCD 北大核心 2022年第7期770-776,共7页
目的探讨索拉非尼抗肝癌的分子机制。方法应用肝癌人源肿瘤异体移植(PDX)模型进行索拉非尼药效筛选和验证;采用小动物B型超声和活体激光共聚焦观察PDX血管生成;采用免疫组化观察PDX组织增殖、血管生成相关蛋白的表达;采用实时定量PCR技... 目的探讨索拉非尼抗肝癌的分子机制。方法应用肝癌人源肿瘤异体移植(PDX)模型进行索拉非尼药效筛选和验证;采用小动物B型超声和活体激光共聚焦观察PDX血管生成;采用免疫组化观察PDX组织增殖、血管生成相关蛋白的表达;采用实时定量PCR技术观察PDX组织Runt相关转录因子3(RUNX3)基因表达;采用SPSS 17.0统计软件进行统计学分析。结果用4例PDX进行索拉非尼药效筛选,PDX1对索拉非尼有明显应答,抑制率为68.07%;与对照组相比,索拉非尼明显抑制PDX1相对肿瘤体积(5.76±2.14比11.71±2.87,P<0.05);细胞分裂指数(39.50±7.72比67.10±9.14,P<0.05)以及Ki67表达明显降低(288.60±43.40比531.70±55.60,P<0.05);小动物超声可以检测到PDX1肿瘤有明显血流信号;活体激光共聚焦结果显示索拉非尼能明显减低PDX1肿瘤的总血管长度(1573.00±236.21比2675.03±162.00,P<0.05)和面积(11145.33±1931.97比20105.37±885.93,P<0.05);免疫组织化学结果显示索拉非尼显著下调CD34(27.55±3.76比45.47±5.57,P<0.05)和血管内皮生长因子(VEGF)(16.33±2.86比22.77±3.20,P<0.05)蛋白表达以及减少微血管密度(38.75±6.01比55.50±8.61,P<0.05);Real-time PCR结果显示索拉非尼明显上调RUNX3基因表达(2.14±0.71比1.00±0.36,P<0.05),索拉非尼组RUNX3基因表达量与VEGF阳性细胞比率呈负相关(R^(2)=0.5097)。结论索拉非尼可能通过调控RUNX3-VEGF通路抑制肝癌PDX血管生成进而抑制肝癌生长。 展开更多
关键词 索拉非尼 肝癌 血管生成 RUNT相关转录因子3 人源肿瘤异体移植
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