[Objective] The study aimed at analyzing Genetic Differentiation of Cervinae. [Method] The complete mitochondrial DNA control region of five species of Cervinae was determined by direct DNA sequencing. Homologous sequ...[Objective] The study aimed at analyzing Genetic Differentiation of Cervinae. [Method] The complete mitochondrial DNA control region of five species of Cervinae was determined by direct DNA sequencing. Homologous sequences of others Cervinae were gained from GenBank, and then analyzed through biology software. [Result] The lengths of their control region were 921-1 072 bp, the nucleotide content of T, A, C, and G was 32.1%, 30.2%, 22.7% and 15.0% separately. Genetic distance of each species ranged from 0.062-0.106, which belonged to level of inter-genus. The molecular phylogenetic tree indicated that red deer, skia deer and White-lipped Deer had close relationship, and they constituted one branch with sambar deer. Eld＇s deer and elk deer constituted the second branch, and hog deer constituted the third branch alone. [Conclusion] Hog deer and fallow deer should be incorporated in Cervus, divergence time is about 1.55-2.60 million years among Cervinae in China.展开更多
Pea ( Pisum sativum Linn.) actin gene family contains at least three isoforms (PEAcⅠ, PEAcⅡand PEAcⅢ), and the DNA sequence of these isoforms show high similarity in the coding regions and significant divergence...Pea ( Pisum sativum Linn.) actin gene family contains at least three isoforms (PEAcⅠ, PEAcⅡand PEAcⅢ), and the DNA sequence of these isoforms show high similarity in the coding regions and significant divergence in the untranslated regions. RT_PCR and Southern blotting using 3′_untranslated region (3′_UTR) as specific probe revealed that pea isoactin genes were expressed in roots, stems, leaves, tendrils, pollen and juvenile siliques, but displayed different patterns of transcript accumulation. Two_fold serial dilution electrophoresis showed PEAcⅠ mRNA preferentially accumulated in rapidly developing tissues: it peaked in seven days' stem; remained at a rather high level in leaves within a month but decreased significantly later; varied a little in tendrils and reached a median and a very low level respectively in juvenile siliques and in pollen. PEAcⅡ displayed somewhat similar expression pattern to PEAcⅠ. The observed differences in sequences and transcript accumulation patterns suggest that the individual pea actin genes may differ in their transcriptional regulation and cellular function. Phylogenetic tree of actins showed that pea actin isoforms are as diverged from each other as they are from other plant actins, and pea actins might have originated from a common ancestor before the divergence of the dicot and monocot plants.展开更多
The vector transformation and pole reduction from the total-field anomaly are signifi cant for the interpretation.We examined these industry-standard processing procedures in the Fourier domain.We propose a novel iter...The vector transformation and pole reduction from the total-field anomaly are signifi cant for the interpretation.We examined these industry-standard processing procedures in the Fourier domain.We propose a novel iteration algorithm for regional magnetic anomalies transformations to derive the vertical-component data from the total-field measurements with the variation in the core-fi eld direction over the region.Additionally,we use the same algorithm to convert the calculated vertical-component data into the corresponding data at the pole and realize the processing of diff erential reduction to the pole(DRTP).Unlike Arkani-Hamed’s DRTP method,the two types of iterative algorithms have the same forms,and DRTP is realized by implementing this algorithm twice.The synthetic model’s calculation results show that the method has high accuracy,and the fi eld data processing confi rms its practicality.展开更多
We compared the cDNA sequences and amino acid sequences of Chalcone synthase （CHS）, Chalcone isomerase （CH1）, and lsoflavone synthase （IFS） in isoflavone biosynthesis pathway, found that their similarity were no...We compared the cDNA sequences and amino acid sequences of Chalcone synthase （CHS）, Chalcone isomerase （CH1）, and lsoflavone synthase （IFS） in isoflavone biosynthesis pathway, found that their similarity were not always consistent; then we compared the amino acid sequences of CHS, CHI, and IFS, found that the clustering relationship of CHS and CHI were different in 13 kind of species; CHS and IFS were the same or similar in leguminous plants Medicago sativa, Medicago truncatula, Pisum sativum, Pueraria Montana, and Glycine max however disaccording with CHI. We prefer the amino acid sequence for cDNA sequence for evolution analysis, and think that the secondary metabolism mostly attributing to environmental stresses, and the functional genes on secondary metabolism pathway are unsuitable for taxonomy analysis.展开更多
This paper proposes an integrative scheme of DiffServ （differentiated service） for the IP-based network. In the scheme, QoS （Quality of Service） is ranked according to the level of both the queuing delay and the d...This paper proposes an integrative scheme of DiffServ （differentiated service） for the IP-based network. In the scheme, QoS （Quality of Service） is ranked according to the level of both the queuing delay and the drop precedence that base on the relative differentiated service; it does not take the resource reservation problem into consideration for making its implementation more simple and flexible. We will propose an implemented architecture including edge routers and core routers, and the PHB （per-hop-behavior） architecture of the routers is generalized. We adopt ERED （extended random early discard） mechanism for drop precedence and use proportional delay differentiation for queuing delay. In the proportional delay differentiation topic, we propose a new WTP （waiting time priority）-like algorithm. Besides, a new stochastic petri net model of the PHB model is given, and the performance of whole implemented PHB architecture is analyzed, too.展开更多
基金Supported by National Science and Technology Support Program(2007BAI38B03)National Platform Project for Natural Science and Technology Rescources(No.2004DKA30460)~~
文摘[Objective] The study aimed at analyzing Genetic Differentiation of Cervinae. [Method] The complete mitochondrial DNA control region of five species of Cervinae was determined by direct DNA sequencing. Homologous sequences of others Cervinae were gained from GenBank, and then analyzed through biology software. [Result] The lengths of their control region were 921-1 072 bp, the nucleotide content of T, A, C, and G was 32.1%, 30.2%, 22.7% and 15.0% separately. Genetic distance of each species ranged from 0.062-0.106, which belonged to level of inter-genus. The molecular phylogenetic tree indicated that red deer, skia deer and White-lipped Deer had close relationship, and they constituted one branch with sambar deer. Eld＇s deer and elk deer constituted the second branch, and hog deer constituted the third branch alone. [Conclusion] Hog deer and fallow deer should be incorporated in Cervus, divergence time is about 1.55-2.60 million years among Cervinae in China.
文摘Pea ( Pisum sativum Linn.) actin gene family contains at least three isoforms (PEAcⅠ, PEAcⅡand PEAcⅢ), and the DNA sequence of these isoforms show high similarity in the coding regions and significant divergence in the untranslated regions. RT_PCR and Southern blotting using 3′_untranslated region (3′_UTR) as specific probe revealed that pea isoactin genes were expressed in roots, stems, leaves, tendrils, pollen and juvenile siliques, but displayed different patterns of transcript accumulation. Two_fold serial dilution electrophoresis showed PEAcⅠ mRNA preferentially accumulated in rapidly developing tissues: it peaked in seven days' stem; remained at a rather high level in leaves within a month but decreased significantly later; varied a little in tendrils and reached a median and a very low level respectively in juvenile siliques and in pollen. PEAcⅡ displayed somewhat similar expression pattern to PEAcⅠ. The observed differences in sequences and transcript accumulation patterns suggest that the individual pea actin genes may differ in their transcriptional regulation and cellular function. Phylogenetic tree of actins showed that pea actin isoforms are as diverged from each other as they are from other plant actins, and pea actins might have originated from a common ancestor before the divergence of the dicot and monocot plants.
基金supported by the National Key R&D Program of China (No. 2017YFC0602000)the China Geological Survey Project (Nos. DD20191001 and DD20189410)。
文摘The vector transformation and pole reduction from the total-field anomaly are signifi cant for the interpretation.We examined these industry-standard processing procedures in the Fourier domain.We propose a novel iteration algorithm for regional magnetic anomalies transformations to derive the vertical-component data from the total-field measurements with the variation in the core-fi eld direction over the region.Additionally,we use the same algorithm to convert the calculated vertical-component data into the corresponding data at the pole and realize the processing of diff erential reduction to the pole(DRTP).Unlike Arkani-Hamed’s DRTP method,the two types of iterative algorithms have the same forms,and DRTP is realized by implementing this algorithm twice.The synthetic model’s calculation results show that the method has high accuracy,and the fi eld data processing confi rms its practicality.
文摘We compared the cDNA sequences and amino acid sequences of Chalcone synthase （CHS）, Chalcone isomerase （CH1）, and lsoflavone synthase （IFS） in isoflavone biosynthesis pathway, found that their similarity were not always consistent; then we compared the amino acid sequences of CHS, CHI, and IFS, found that the clustering relationship of CHS and CHI were different in 13 kind of species; CHS and IFS were the same or similar in leguminous plants Medicago sativa, Medicago truncatula, Pisum sativum, Pueraria Montana, and Glycine max however disaccording with CHI. We prefer the amino acid sequence for cDNA sequence for evolution analysis, and think that the secondary metabolism mostly attributing to environmental stresses, and the functional genes on secondary metabolism pathway are unsuitable for taxonomy analysis.
文摘This paper proposes an integrative scheme of DiffServ （differentiated service） for the IP-based network. In the scheme, QoS （Quality of Service） is ranked according to the level of both the queuing delay and the drop precedence that base on the relative differentiated service; it does not take the resource reservation problem into consideration for making its implementation more simple and flexible. We will propose an implemented architecture including edge routers and core routers, and the PHB （per-hop-behavior） architecture of the routers is generalized. We adopt ERED （extended random early discard） mechanism for drop precedence and use proportional delay differentiation for queuing delay. In the proportional delay differentiation topic, we propose a new WTP （waiting time priority）-like algorithm. Besides, a new stochastic petri net model of the PHB model is given, and the performance of whole implemented PHB architecture is analyzed, too.
