AIM： TO accurately and realistically elucidate human stem cell behaviors in vivo and the fundamental mechanisms controlling human stem cell fates in vivo, which is urgently required in regenerative medicine and treat...AIM： TO accurately and realistically elucidate human stem cell behaviors in vivo and the fundamental mechanisms controlling human stem cell fates in vivo, which is urgently required in regenerative medicine and treatments for some human diseases, a surrogate human-rat chimera model was developed. METHODS： Human-rat chimeras were achieved by in utero transplanting low-density mononuclear cells from human umbilical cord blood into the fetal rats at 9-11 d of gestation, and subsequently, a variety of methods, including flow cytometry, PCR as well as immunohistochemical assay, were used to test the human donor contribution in the recipients. RESULTS： Of 29 live-born recipients, 19 had the presence of human CD45^＋ cells in peripheral blood （PB） detected by flow cytometry, while PCR analysis on genomic DNA from 11 different adult tissues showed that 14 selected from flow cytometry-positive 19 animals possessed of donor-derived human cell engraftment in multiple tissues （i.e. liver, spleen, thymus, heart, kidney, blood, lung, muscle, gut and skin） examined at the time of tissue collection, as confirmed by detecting human 132- microglobulin expression using immunohistochemistry. Tn this xenogeneic system, the engrafted donor-derived human cells persisted in multiple tissues for at least 6 mo after birth. Moreover, transplanted human donor cells underwent site-specific differentiation into CK18-positive human cells in chimeric liver and CEHS-positive human cells in chimeric spleen and thymus of recipients. CONCLUSION： Taken together, these findings suggest that we successfully developed human-rat chimeras, in which xenogeneic human cells exist up to 6 mo later. This humanized small animal model, which offers an in vivo environment more closely resembling to the situations in human, provides an invaluable and effective approach for in vivo investigating human stem cell behaviors, and further in vivo examining fundamental mechanisms controlling human stem cell fates in the future. The potential for new advances in our better understanding the living biological systems in human provided by investigators in humanized animals will remain promising.展开更多
Interannual variations of Pacific North Equatorial Current （NEC） transport during eastern- Pacific E1 Nifios （EP-EI Nifios） and central-Pacific E1 Nifios （CP-E1 Nifios） are investigated by composite analysis wit...Interannual variations of Pacific North Equatorial Current （NEC） transport during eastern- Pacific E1 Nifios （EP-EI Nifios） and central-Pacific E1 Nifios （CP-E1 Nifios） are investigated by composite analysis with European Centre for Medium-Range Weather Forecast Ocean Analysis/Reanalysis System 3. During EP-E1 Nifio, NEC transport shows significant positive anomalies from the developing to decay phases, with the largest anomalies around the mature phase. During CP-EI Nifio, however, the NEC transport only shows positive anomalies before the mature phase, with much weaker anomalies than those during EP-El Nifio. The NEC transport variations are strongly associated with variations of the tropical gyre and wind forcing in the tropical North Pacific. During EP-E1 Nifio, strong westerly wind anomalies and positive wind stress curl anomalies in the tropical North Pacific induce local upward Ekman pumping and westward-propagating upwelling Rossby waves in the ocean, lowering the sea surface height and generating a cyclonic gyre anomaly in the western tropical Pacific. During CP-E1 Nifio, however, strength of the wind and associated Ekman pumping velocity are very weak. Negative sea surface height and cyclonic flow anomalies are slightly north of those during EP El Nino.展开更多
Insufficient N supply is an important constraint to productivity of lowland rice. Studies on N nutrition of rice cultivars with different durations representing the north western part of Indo-Gangetic Plains are scant...Insufficient N supply is an important constraint to productivity of lowland rice. Studies on N nutrition of rice cultivars with different durations representing the north western part of Indo-Gangetic Plains are scanty. A field experiment was conducted during kharif seasons of 2006 and 2007 at PAU, Ludhiana to assess the differences in grain yield and N utilization of three popular rice cultivars at varying N doses. Significant differences among genotypes were observed in grain yield, N uptake, N use efficiency and N utilization efficiency. The cultivar PAU-201 was found to be superior among all the tested genotypes. Total N uptake and grain N uptake was highest in cultivar PAU-201 followed by cultivars PR-115 and PR-113. On an average, the response to applied N for grain yield was observed upto 90 kg N ha1. Grain yield increased significantly up to 90 kg N hal in cultivars PR-115 and PAU-201 and up to 120 kg N ha1 in PR-113. Apparent recovery efficiency （ARE） and Partial factor productivity （PFPN） of N was significantly reduced at higher level of N （150 kg N hal）. It was concluded that N uptake is predominant factor in grain yield formation and cultivars differ in NUE suggesting that it may be possible to develop cultivars that are efficient at low nutrient level or are capable of using N more efficiently when applied as fertilizer.展开更多
基金Supported by The National Natural Science Foundation of China, No. 30271177 and No. 39870676 the National 9th Five-year Program, No. 101033+3 种基金 The Major Science and Technology Projects of Guangdong Province, No. B602 Natural Science Foundation of Guangdong Province, No. 021903 The Postdoctoral Fellowship Foundation of China (Series 29)The Special Fund of Scientifi c Instrument Collaborative Share-net in Guangzhou, No. 2006176
文摘AIM： TO accurately and realistically elucidate human stem cell behaviors in vivo and the fundamental mechanisms controlling human stem cell fates in vivo, which is urgently required in regenerative medicine and treatments for some human diseases, a surrogate human-rat chimera model was developed. METHODS： Human-rat chimeras were achieved by in utero transplanting low-density mononuclear cells from human umbilical cord blood into the fetal rats at 9-11 d of gestation, and subsequently, a variety of methods, including flow cytometry, PCR as well as immunohistochemical assay, were used to test the human donor contribution in the recipients. RESULTS： Of 29 live-born recipients, 19 had the presence of human CD45^＋ cells in peripheral blood （PB） detected by flow cytometry, while PCR analysis on genomic DNA from 11 different adult tissues showed that 14 selected from flow cytometry-positive 19 animals possessed of donor-derived human cell engraftment in multiple tissues （i.e. liver, spleen, thymus, heart, kidney, blood, lung, muscle, gut and skin） examined at the time of tissue collection, as confirmed by detecting human 132- microglobulin expression using immunohistochemistry. Tn this xenogeneic system, the engrafted donor-derived human cells persisted in multiple tissues for at least 6 mo after birth. Moreover, transplanted human donor cells underwent site-specific differentiation into CK18-positive human cells in chimeric liver and CEHS-positive human cells in chimeric spleen and thymus of recipients. CONCLUSION： Taken together, these findings suggest that we successfully developed human-rat chimeras, in which xenogeneic human cells exist up to 6 mo later. This humanized small animal model, which offers an in vivo environment more closely resembling to the situations in human, provides an invaluable and effective approach for in vivo investigating human stem cell behaviors, and further in vivo examining fundamental mechanisms controlling human stem cell fates in the future. The potential for new advances in our better understanding the living biological systems in human provided by investigators in humanized animals will remain promising.
基金Supported by the National Basic Research Program of China(973 Program)(Nos.2012CB417401,2013CB956202)the Key Project of National Natural Science Foundation of China(No.41330963)the Fundamental Research Funds for the Central Universities(No.201513030)
文摘Interannual variations of Pacific North Equatorial Current （NEC） transport during eastern- Pacific E1 Nifios （EP-EI Nifios） and central-Pacific E1 Nifios （CP-E1 Nifios） are investigated by composite analysis with European Centre for Medium-Range Weather Forecast Ocean Analysis/Reanalysis System 3. During EP-E1 Nifio, NEC transport shows significant positive anomalies from the developing to decay phases, with the largest anomalies around the mature phase. During CP-EI Nifio, however, the NEC transport only shows positive anomalies before the mature phase, with much weaker anomalies than those during EP-El Nifio. The NEC transport variations are strongly associated with variations of the tropical gyre and wind forcing in the tropical North Pacific. During EP-E1 Nifio, strong westerly wind anomalies and positive wind stress curl anomalies in the tropical North Pacific induce local upward Ekman pumping and westward-propagating upwelling Rossby waves in the ocean, lowering the sea surface height and generating a cyclonic gyre anomaly in the western tropical Pacific. During CP-E1 Nifio, however, strength of the wind and associated Ekman pumping velocity are very weak. Negative sea surface height and cyclonic flow anomalies are slightly north of those during EP El Nino.
文摘Insufficient N supply is an important constraint to productivity of lowland rice. Studies on N nutrition of rice cultivars with different durations representing the north western part of Indo-Gangetic Plains are scanty. A field experiment was conducted during kharif seasons of 2006 and 2007 at PAU, Ludhiana to assess the differences in grain yield and N utilization of three popular rice cultivars at varying N doses. Significant differences among genotypes were observed in grain yield, N uptake, N use efficiency and N utilization efficiency. The cultivar PAU-201 was found to be superior among all the tested genotypes. Total N uptake and grain N uptake was highest in cultivar PAU-201 followed by cultivars PR-115 and PR-113. On an average, the response to applied N for grain yield was observed upto 90 kg N ha1. Grain yield increased significantly up to 90 kg N hal in cultivars PR-115 and PAU-201 and up to 120 kg N ha1 in PR-113. Apparent recovery efficiency （ARE） and Partial factor productivity （PFPN） of N was significantly reduced at higher level of N （150 kg N hal）. It was concluded that N uptake is predominant factor in grain yield formation and cultivars differ in NUE suggesting that it may be possible to develop cultivars that are efficient at low nutrient level or are capable of using N more efficiently when applied as fertilizer.
