Human embryonic fibroblasts （hEFs） can well maintain the pluripotency in human embryonic stem cells （hESs）. However, recent research and reports indicated that a few of hES cell lines acquired genomic alteration d...Human embryonic fibroblasts （hEFs） can well maintain the pluripotency in human embryonic stem cells （hESs）. However, recent research and reports indicated that a few of hES cell lines acquired genomic alteration during long-term culture of hES cells in vitro. This will directly restrict the therapy use of hES cells. Wnts are secreted lipid-modified signaling proteins that influence multiple processes ranging from cell proliferation to stem cell loss. Activation of Wnt signaling in many tissues has also been associated with cancer. Unchecked Wnt signaling and loss of cadherin expression can promote tumorigenesis. In this study, we found the caryotype of one hES cell line chHES-3 changed with duplication of 1 p32-1p36 area after 34 passages. The results of RT-PCR indicated Wnt7a was expressed in hEFs after culture normal karyotype hES cells, but not expressed in control and abnormal karyotype hES cells. Wnt3 was expressed in hEFs after culture abnormal karyotype hES cells, not expressed in control and normal karyotype hES cells. Wnt3, Wnt9a and WntlOb were detected weakly expression in normal hES cells, but higher in abnormal hES cells. At the same time, Wnt3a, Wnt4, Wnt5b, Wnt7a, Wnt8b and Wnt11 were expressed and E-cadherin was not tested in abnormal hES cells compared with normal hES cells. All that indicated Wnt7a was need for culture normal karyotype hES cells and Wnt3 was need for culture abnormal karyotype hES cells on hEFs. Wnt3, Wnt9a and WntlOb high expression in hES cells and absence of E-cadherin may cause hES cells karyotype change.展开更多
Objective To analyze the relationship between karyotypes and clinic features of patients with primary amenorrhea.Methods G banding was done for 340 patients with primary amenorrhea to facilitate individual chromosome ...Objective To analyze the relationship between karyotypes and clinic features of patients with primary amenorrhea.Methods G banding was done for 340 patients with primary amenorrhea to facilitate individual chromosome identification,and if specific staining for certain portions of the chromosome was necessary,C banding was used.The clinical data were recorded by physical examination and ultrasound scanning.Results Karyotype analysis of the 340 patients revealed that 180(52.94%) patients had normal female karyotypes and 160(47.06%) patients had abnormal karyotypes.The abnormal karyotypes included abnormal X chromosome(150 patients),mosaic X-Y chromosome(4 patients),abnormal autosome(5 patients),and X-autosome translocation(1 patient).The main clinical manifestations in patients with primary amenorrhea were primordial or absent uterus(95.9%),invisible secondary sex features(68.8%),little or absent ovary(62.6%),and short stature(30.0%).The incidence of short stature in patients with X chromosome aberration(46%,69/150) was significangly higher that in patients with 46,XX(9.44%,17/180) as well as 46,XY(6.67%,3/45;χ2=146.25,P=0.000).All primary amenorrhea patients with deletion or break-point at Xp11.1-11.4 were short statures.Conclusions One of the main reasons of primary amenorrhea is choromosome abnormality,especially heterosome abnormality.It implies the need to routinely screen chromosomal anomalies for such patients.There might be relationship between Xp11.1-11.4 integrity and height improvement.展开更多
This paper studies the imminent anomalies observed by the Sacks volume strainmeter in Erzhangying station and Tiantanghe station before 80 earthquakes with Ms≥ 7. 0 which took place from January 2011 to April 2014 al...This paper studies the imminent anomalies observed by the Sacks volume strainmeter in Erzhangying station and Tiantanghe station before 80 earthquakes with Ms≥ 7. 0 which took place from January 2011 to April 2014 all over the world. Then, preconditions for anomaly identification are put forward for complex earthquake cases. Statistical results show that volume strain observation has a better earthquake reflecting ability for earthquakes with magnitudes larger than 7. 0 and epicentral distance within 8000kin. In addition, these results also reflect that the volume strain observation can better reflect precursory anomalies of such earthquakes. Based on categorization and description of those anomalies, we divide the anomalies into three types, that is, earth tide distortion type, abrupt change type and slow earthquake type. Furthermore, the paper makes a statistical analysis of these types and preliminarily discusses their mechanical properties as well. According to research, volume strain anomaly has an indicative significance to future strong earthquakes in the world.展开更多
基金Acknowledgment This work was supported by grants from the National Nature Science Foundation of China （No.31071091 No.30971570） and Department of Education key project of Hunan Province, China （09A035 and 07B029）.
文摘Human embryonic fibroblasts （hEFs） can well maintain the pluripotency in human embryonic stem cells （hESs）. However, recent research and reports indicated that a few of hES cell lines acquired genomic alteration during long-term culture of hES cells in vitro. This will directly restrict the therapy use of hES cells. Wnts are secreted lipid-modified signaling proteins that influence multiple processes ranging from cell proliferation to stem cell loss. Activation of Wnt signaling in many tissues has also been associated with cancer. Unchecked Wnt signaling and loss of cadherin expression can promote tumorigenesis. In this study, we found the caryotype of one hES cell line chHES-3 changed with duplication of 1 p32-1p36 area after 34 passages. The results of RT-PCR indicated Wnt7a was expressed in hEFs after culture normal karyotype hES cells, but not expressed in control and abnormal karyotype hES cells. Wnt3 was expressed in hEFs after culture abnormal karyotype hES cells, not expressed in control and normal karyotype hES cells. Wnt3, Wnt9a and WntlOb were detected weakly expression in normal hES cells, but higher in abnormal hES cells. At the same time, Wnt3a, Wnt4, Wnt5b, Wnt7a, Wnt8b and Wnt11 were expressed and E-cadherin was not tested in abnormal hES cells compared with normal hES cells. All that indicated Wnt7a was need for culture normal karyotype hES cells and Wnt3 was need for culture abnormal karyotype hES cells on hEFs. Wnt3, Wnt9a and WntlOb high expression in hES cells and absence of E-cadherin may cause hES cells karyotype change.
文摘Objective To analyze the relationship between karyotypes and clinic features of patients with primary amenorrhea.Methods G banding was done for 340 patients with primary amenorrhea to facilitate individual chromosome identification,and if specific staining for certain portions of the chromosome was necessary,C banding was used.The clinical data were recorded by physical examination and ultrasound scanning.Results Karyotype analysis of the 340 patients revealed that 180(52.94%) patients had normal female karyotypes and 160(47.06%) patients had abnormal karyotypes.The abnormal karyotypes included abnormal X chromosome(150 patients),mosaic X-Y chromosome(4 patients),abnormal autosome(5 patients),and X-autosome translocation(1 patient).The main clinical manifestations in patients with primary amenorrhea were primordial or absent uterus(95.9%),invisible secondary sex features(68.8%),little or absent ovary(62.6%),and short stature(30.0%).The incidence of short stature in patients with X chromosome aberration(46%,69/150) was significangly higher that in patients with 46,XX(9.44%,17/180) as well as 46,XY(6.67%,3/45;χ2=146.25,P=0.000).All primary amenorrhea patients with deletion or break-point at Xp11.1-11.4 were short statures.Conclusions One of the main reasons of primary amenorrhea is choromosome abnormality,especially heterosome abnormality.It implies the need to routinely screen chromosomal anomalies for such patients.There might be relationship between Xp11.1-11.4 integrity and height improvement.
基金Combined project of monitoring,prediction and research of China Earthquake Administration entitled "Statistical study on the earthquake cases of imminent anomaly in Sacks volume strain measurements(154201)""Special Fund for Basic Scientific Research of Institute of Earthquake Science,CEA(2012IES010202)""Research on Regional Tectonic Stability for the Dateng Gorge Water Conservancy Hub Project Site(880456)"
文摘This paper studies the imminent anomalies observed by the Sacks volume strainmeter in Erzhangying station and Tiantanghe station before 80 earthquakes with Ms≥ 7. 0 which took place from January 2011 to April 2014 all over the world. Then, preconditions for anomaly identification are put forward for complex earthquake cases. Statistical results show that volume strain observation has a better earthquake reflecting ability for earthquakes with magnitudes larger than 7. 0 and epicentral distance within 8000kin. In addition, these results also reflect that the volume strain observation can better reflect precursory anomalies of such earthquakes. Based on categorization and description of those anomalies, we divide the anomalies into three types, that is, earth tide distortion type, abrupt change type and slow earthquake type. Furthermore, the paper makes a statistical analysis of these types and preliminarily discusses their mechanical properties as well. According to research, volume strain anomaly has an indicative significance to future strong earthquakes in the world.
