Objective： The aim of the study was to observe the effect of total isoflavones from pueraria Iobata （TIP） on D2 dopamine receptor mRNA, preproenkephalin mRNA and prodynorphin mRNA expressions in Parkinson＇s disea...Objective： The aim of the study was to observe the effect of total isoflavones from pueraria Iobata （TIP） on D2 dopamine receptor mRNA, preproenkephalin mRNA and prodynorphin mRNA expressions in Parkinson＇s disease （PD） model cells induced by 1-methyl-4-phenylpyridinium ion （MPP^＋）. Methods： TIP was dissolved in 0.1 M NaOH and added to the culture medium at a final concentrations of 50 mg/L, 100 mg/L and 200 mg/L. Some cells （control） were exposed to 0.001 M NaOH. TIP was added to PC12 cells 30 min prior to the administration of MPP^＋. TIP and MPP^＋ remained in the culture medium for 96 h. D2 dopamine receptor mRNA, preproenkephalin mRNA and prodynorphin mRNA expressions were assayed by real-time quantitative reverse transcription-PCR. Results： The D2 dopamine receptor mRNA and preproenkephalin mRNA expressions were up-regulated in MPP^＋ group compared with the control group, and prodynorphin mRNA expression was down-regulated in that. The D2 dopamine receptor mRNA expression being down-regulated and prodynorphin mRNA expression being up-regulated in TIP group compared with the MPP^＋ group. And there was no effect of TIP on preproenkephalin gene expression in PC12 cells induced by MPP^＋. Conclusion： The results suggest that TIP down-regulates the D2 dopamine receptor mRNA expression, up-regulates prodynorphin mRNA expression and not affects preproenkephalin gene expression in PC12 cells induced by MPP^＋.展开更多
The physico-chemical and biological （algal and bacterial） quality of tap water in Tanta City were elucidated. Samples were collected during December 2011, covering all the water plants and the areal extension of the...The physico-chemical and biological （algal and bacterial） quality of tap water in Tanta City were elucidated. Samples were collected during December 2011, covering all the water plants and the areal extension of the pipe line network in Tanta. Total dissolved solids content classifies water origin into surface water, shallow and deep groundwater. Fe, Mn and hardness in some samples of groundwater origin are obviously exceeding the WHO （World Health Organization） limit. Mostly, green algae are found （31 species）, followed by diatoms （19 species）, then blue-green algae （13 species）. CCA （Canonical Corresponding Analysis） indicates that green algae are strongly correlated with pH, NH4, alkalinity, Mn and Si; diatoms with EC （electric conductivity）, turbidity, Zn and Si and blue green algae with EC and alkalinity. 50% of samples of surface origin, 80% of samples of both deep and shallow groundwater origins crossed WHO and Egyptian guideline in their content of mean TCC （Total Coliform Count）. CCA estimates strong correlations of TCC with temperature, NH4 and PO4; E. coli with EC; heterotrophic bacteria with turbidity and NO3; Salmonella sp. with Si and SO4 and Pseudomonas aeruginosa with Mn. Finally, the water plants treatment may be not effective and pipes serve as a reservoir for pathogenic microorganisms.展开更多
基金Supported by the grants from the National Natural Science Foundation of China (No. 30873396)the National Science Foundation for Postdoctoral Scientists of China (No. 20080430140)+1 种基金Research Foundation of Education Bureau of Heilongjiang Province (No. 11511455)the Qiqihar Foundation for Development of Science and Technology, China(No. SF-08002)
文摘Objective： The aim of the study was to observe the effect of total isoflavones from pueraria Iobata （TIP） on D2 dopamine receptor mRNA, preproenkephalin mRNA and prodynorphin mRNA expressions in Parkinson＇s disease （PD） model cells induced by 1-methyl-4-phenylpyridinium ion （MPP^＋）. Methods： TIP was dissolved in 0.1 M NaOH and added to the culture medium at a final concentrations of 50 mg/L, 100 mg/L and 200 mg/L. Some cells （control） were exposed to 0.001 M NaOH. TIP was added to PC12 cells 30 min prior to the administration of MPP^＋. TIP and MPP^＋ remained in the culture medium for 96 h. D2 dopamine receptor mRNA, preproenkephalin mRNA and prodynorphin mRNA expressions were assayed by real-time quantitative reverse transcription-PCR. Results： The D2 dopamine receptor mRNA and preproenkephalin mRNA expressions were up-regulated in MPP^＋ group compared with the control group, and prodynorphin mRNA expression was down-regulated in that. The D2 dopamine receptor mRNA expression being down-regulated and prodynorphin mRNA expression being up-regulated in TIP group compared with the MPP^＋ group. And there was no effect of TIP on preproenkephalin gene expression in PC12 cells induced by MPP^＋. Conclusion： The results suggest that TIP down-regulates the D2 dopamine receptor mRNA expression, up-regulates prodynorphin mRNA expression and not affects preproenkephalin gene expression in PC12 cells induced by MPP^＋.
文摘The physico-chemical and biological （algal and bacterial） quality of tap water in Tanta City were elucidated. Samples were collected during December 2011, covering all the water plants and the areal extension of the pipe line network in Tanta. Total dissolved solids content classifies water origin into surface water, shallow and deep groundwater. Fe, Mn and hardness in some samples of groundwater origin are obviously exceeding the WHO （World Health Organization） limit. Mostly, green algae are found （31 species）, followed by diatoms （19 species）, then blue-green algae （13 species）. CCA （Canonical Corresponding Analysis） indicates that green algae are strongly correlated with pH, NH4, alkalinity, Mn and Si; diatoms with EC （electric conductivity）, turbidity, Zn and Si and blue green algae with EC and alkalinity. 50% of samples of surface origin, 80% of samples of both deep and shallow groundwater origins crossed WHO and Egyptian guideline in their content of mean TCC （Total Coliform Count）. CCA estimates strong correlations of TCC with temperature, NH4 and PO4; E. coli with EC; heterotrophic bacteria with turbidity and NO3; Salmonella sp. with Si and SO4 and Pseudomonas aeruginosa with Mn. Finally, the water plants treatment may be not effective and pipes serve as a reservoir for pathogenic microorganisms.
