Hypermethylation and aberrant expression of secreted fizzled-related protein genes in pancreatic cancer

AIM：To determine the methylation status and aberrant expression of some secreted frizzled-related protein（SFRP） genes in pancreatic cancer and explore their role in pancreatic carcinogenesis.METHODS：Methylation status and expression of SFRP genes were detected by methylation-specific PCR（MSPCR） and reverse-transcription PCR（RT-PCR） respectively.RESULTS：The frequencies of methylation for SFRP genes 1,2,4,5 were 70%,48.3%,60% and 76.7% in pancreatic cancer samples,and 21.7%,20%,10% and 36.7% in matched cancer adjacent normal tissue samples,respectively（χ^2 = 28.23,P 〈 0.0001 for SFRP gene 1;χ^2 = 10.71,P = 0.001 for SFRP gene 2;χ^2 = 32.97,P 〈 0.0001 for SFRP gene 4;χ^2 = 19.55,P 〈 0.0001 for SFRP gene 5）.Expression loss of SFRP genes 1,2,4 and 5 was found in 65%,40%,55% and 71.7% of 60 pancreatic cancer samples,and 25%,15%,18.3% and 31.7% of matched cancer adjacent normal tissue samples,respectively（χ^2 = 19.39,P 〈 0.0001 for SFRP gene 1;χ^2 = 9.40,P = 0.002 for SFRP gene 2;χ^2 = 17.37,P 〈 0.0001 for SFRP gene 4;χ^2 = 19.22,P 〈 0.0001 for SFRP gene 5）.SFRP gene 1 was methylated but not expressed in PC-3 and PANC-1,SFRP gene 2 was methylated but not expressed in PANC-1 and CFPAC-1,SFRP gene 4 was methylated but not expressed in PC-3,and SFRP gene 5was methylated but not expressed in CFPAC-1.CONCLUSION： Hypermethylation and aberrant expression of SFRP genes are common in pancreatic cancer, which may be involved in pancreatic carcinogenesis.

回顾性的前瞻:“后理论”时代的“比较诗学”

如果西方理论无法在“类同疗法”的惯性下完成自我超越,那么内蕴“异质疗法”的“比较诗学”或可成为其自反运动的新起点。因为在“比较诗学”的后设性视域中,作为“局域诗学”的西方诗学(也包括西方理论)并无规范性的特权。所以,“后理论”时代的“比较诗学”,理应成为多元文化之间积极重组的开放性场域和功能性枢纽。