AIM： TO study immune response induced by foreign plasmid DNA after oral administration in mice.METHODS： Mice were orally administered with 200 μg of plasmid pcDNA3 once and spleen was isolated 4 h and 18 h after ad...AIM： TO study immune response induced by foreign plasmid DNA after oral administration in mice.METHODS： Mice were orally administered with 200 μg of plasmid pcDNA3 once and spleen was isolated 4 h and 18 h after administration. Total RNA was extracted from spleen and gene expression profile of BALB/c mice spleen was analyzed by using Affyrnetrix oligonucleotide GeneChip. Functional cluster analysis was conducted by GenNAPP software.RESULTS： At 4 h and 18 h after oral plasmid pcDNA3 administration, a number of immune-related genes, including cytokine and cytokine receptors, chemokines and chemokine receptor, complement molecule, proteasome, histocompatibility molecule, lymphocyte antigen complex and apoptotic genes, were upregulated. Mloreover, MAPPFinder results also showed that numerous immune response processes were upregulated. In contrast, the immunoglobulin genes were down-regulated.CONCLUSION： Foreign plasmid DNA can modulate the genes expression related to immune system via the gastrointestinal tract, and further analysis of the related immune process may help understand the molecular mechanisms of immune response induced by foreign plasmid via the gastrointestinal tract.展开更多
基金the National Natural Science Foundation of China, No. 30270970
文摘AIM： TO study immune response induced by foreign plasmid DNA after oral administration in mice.METHODS： Mice were orally administered with 200 μg of plasmid pcDNA3 once and spleen was isolated 4 h and 18 h after administration. Total RNA was extracted from spleen and gene expression profile of BALB/c mice spleen was analyzed by using Affyrnetrix oligonucleotide GeneChip. Functional cluster analysis was conducted by GenNAPP software.RESULTS： At 4 h and 18 h after oral plasmid pcDNA3 administration, a number of immune-related genes, including cytokine and cytokine receptors, chemokines and chemokine receptor, complement molecule, proteasome, histocompatibility molecule, lymphocyte antigen complex and apoptotic genes, were upregulated. Mloreover, MAPPFinder results also showed that numerous immune response processes were upregulated. In contrast, the immunoglobulin genes were down-regulated.CONCLUSION： Foreign plasmid DNA can modulate the genes expression related to immune system via the gastrointestinal tract, and further analysis of the related immune process may help understand the molecular mechanisms of immune response induced by foreign plasmid via the gastrointestinal tract.
