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用紫外分光法测定鱼藤中异黄酮类的总含量 被引量:2
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作者 陈欣安 陆磊 《中南林学院学报》 CSCD 1996年第1期46-49,共4页
根据鱼藤中异黄酮类化合物在紫外光210~245nm及290~305nm处具有吸收峰这一特性,提出了用紫外分光法测定鱼藤中异黄酮类总含量的方法.在300nm波长处,浓度低于6×2.618×10-3/mol·... 根据鱼藤中异黄酮类化合物在紫外光210~245nm及290~305nm处具有吸收峰这一特性,提出了用紫外分光法测定鱼藤中异黄酮类总含量的方法.在300nm波长处,浓度低于6×2.618×10-3/mol·L-1时,测定了标准系列的吸光值,结果表明吸光值与浓度近乎直线相关,相关系数为0.9977;标准系列吸光值准确性高;加样回收率达99%以上;测定样品时,重现性好. 展开更多
关键词 鱼藤 异黄酮总量 紫外分光法
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Analysis on Interaction between Genotype of Four Main Flavonoids of Barley Grain and Environment 被引量:1
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作者 杨涛 段承俐 +4 位作者 曾亚文 杜娟 杨树明 普晓英 杨生超 《Agricultural Science & Technology》 CAS 2012年第9期1843-1847,1903,共6页
[Objective] This study aimed to analyze the interaction between genotype of flavonoids of barley grain and environment, to increase the flavonoid content of barley grain in cultivation and breeding. [Method] In this s... [Objective] This study aimed to analyze the interaction between genotype of flavonoids of barley grain and environment, to increase the flavonoid content of barley grain in cultivation and breeding. [Method] In this study, the content of cate- chin, myricetin, quercetin and kaempferol of barley grain planted in Kunming, Qujing and Baoshan were determined by HPLC, and the genotype, environment, genotype- environment interaction of the flavonoid content of barley grain were analyzed. [Result] According to the experimental results, the genotype variance, environmental variance and G x E interaction variance of catechin and kaempferol contents show the same trend: genotype variation 〉 environmental variation 〉 G × E interaction variation, which all reach a extremely significant level; the genotype variance, envi- ronmental variance and G × E interaction variance of quercetin and total flavonoid contents show the same trend: genetype variation 〉 G × E interaction variation 〉 environmental variation, which all reach a extremely significant level; the genotype variance and environmental variance of myricetin content both reach a extremely sig- nificant level, while the G × E interaction variance reaches a significant level, showing an order of genotype variation 〉 environmental variation 〉 G × E interaction variation; the genotype variance, environmental variance and G x E interaction vari- ance of total flavonoid content show an order of genotype variation 〉 environmental variation 〉 G × E interaction variation. Among different barley varieties, Ziguang- mangluoerling and Kuanyingdamai in Qujing, Kunming and Baoshan have relatively high content of quercetin, while other barley varieties barely contain any quercetin. The grains of Ziguangmangluoerling and Kuanyingdamai are purple, while the grains of other barley varieties are yellow. [Conclusion] Four main flavonoids and the total flavonoids of barley grain are mainly under genetic control and affected by genetic- environment interactions; the purple barley grains contain high content of quercetin. 展开更多
关键词 BARLEY HPLC FLAVONOIDS Genotype-environment interaction
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