仙人掌中一个新α-吡喃酮成分的分离与结构鉴定

目的 研究仙人掌的化学成分。方法 应用多种柱色谱方法进行分离和纯化 ,NMR和MS等波谱解析化学结构。结果 从仙人掌肉质茎的乙醇提取物中分离出 6个化合物 ,其结构分别鉴定为 :3 O 甲基异鼠李黄素 (1)、4 乙氧基 6 羟甲基 α 吡喃酮 (2 )、正十七醇 (3)、香草酸 (4)、异鼠李黄素 3 O 鼠李糖苷 (5 )和芦丁 (6 )。结论 化合物2为新化合物 ,化合物 1、3、4、5均为首次从本属植物中分离得到 ,化合物 6为本植物首次分离得到。

食用仙人掌降糖活性成分研究

目的:寻找食用仙人掌中降糖活性的成分。方法:采用硅胶柱层析、制备薄层等方法分离;按70mg.kg-1体重尾静脉注射四氧嘧啶建立小鼠糖尿病模型。分别以200,400mg.kg-1体重食用仙人掌提取物连续灌胃给药10d,于给药第10天检测各小鼠空腹血糖含量。用TLC,MS,NMR等技术对从食用仙人掌降糖有效部位分离得到的成分进行结构鉴定。结果:食用仙人掌提取物对四氧嘧啶糖尿病小鼠血糖的升高有一定的缓解作用。给药10d,给药组与阴性组的血糖值有显著性差异。从新鲜食用仙人掌的块茎中首次分离得到5个化合物,通过波谱分析鉴定为十七醇(1),β-谷甾醇(2),十八烯酸(3),胡萝卜苷(4),异鼠李黄素-3-O-β-D-芸香糖苷(5)。结论:食用仙人掌具有一定的抗糖尿病作用。

Induction of apoptosis in lung cancer cells by isorhamnetin

AIM The aim of the present study was to explore cytotoxic activity and the mechanism of tumor cell killing by isorhamnetin and to investigate the effect of isorhamnetin on tumor growth, cell prolification and apoptosis in transplantation tumor of lung cancer of Lewis cell line in C57BL/6 mice. METHODS Human A549 cells were treated with 10-320(g/ml isorhamnetin, C57BL/6 mice were subcutaneously inoculated Lewis cells 0.2ml/each (1×107cells/ml) below the right forelimb armpit and were treated with 50 (g/ml isorhamnetin isorhamnetin.The results were observed and analyzed under light-microscope, electronic microscopy, growth inhibition was analyzed by MTT, clonogenic asssays and growth curve;the apoptosis and the expression-associated genes peaks were detected with flow cytometry (FCM), DNA fragmentation, single cell gel electrophoresis (comet) assay,