在丝网印刷碳电极(SPCE)表面修饰石墨烯-壳聚糖(GPCS)复合膜和CeO2-Au纳米粒子,利用CeO2-Au纳米粒子对弓形虫特异性抗原(Tg-Ag)的固定,构建了用于弓形虫IgM抗体(Tg-IgM)检测的一次性电流型免疫传感器.采用扫描电镜(SEM)和透射电镜(TEM)...在丝网印刷碳电极(SPCE)表面修饰石墨烯-壳聚糖(GPCS)复合膜和CeO2-Au纳米粒子,利用CeO2-Au纳米粒子对弓形虫特异性抗原(Tg-Ag)的固定,构建了用于弓形虫IgM抗体(Tg-IgM)检测的一次性电流型免疫传感器.采用扫描电镜(SEM)和透射电镜(TEM)对该免疫传感器的修饰进行表征,利用循环伏安法(CV)、交流阻抗法(EIS)和差分脉冲伏安法(DPV)进行电化学性能检测.响应电流与Tg-IgM的浓度在7.5×10-4～24 AU mL-1的范围内呈线性相关,检测限为4.4×10-4AU mL-1.该免疫传感器具有良好的灵敏度、特异性、稳定性和重复性.与ELISA方法相比,该方法结果可靠,孵育时间短,可用于临床上Tg-IgM的检测.展开更多
Several researchers have investigated the association of numerous opportunistic pathogens with HIV, little is documented on its association with T. gondii in our environment. We investigated the prevalence of T. gondi...Several researchers have investigated the association of numerous opportunistic pathogens with HIV, little is documented on its association with T. gondii in our environment. We investigated the prevalence of T. gondii immunoglobulins G and M （IgG and IgM） in HIV positive individuals in relation to their cluster of differentiation 4 （CD4） cells count. IgG, IgM and CD4 were assayed using enzyme immunoassay （EIA） and flowcytometry respectively. 341 HIV positive individuals were studied in the present research, 30 （8.7%） of them had T. gondii IgG and IgM, 297 subjects had CD4 cells count a range of 200-400 cells/μL, 27 （9.7%） and 2 （0.6%） of which had T. gondii IgG and IgM respectively. Of the 44 HIV positive subjects with CD4 〉 400 cells/μL, one （2.2%） was positive for T. gondii IgG. In the control group, all the 177 had CD4 〉 400 cells/μL of which, one （0.5%） had T. gondii IgG. The prevalence of T. gondii infection was significantly higher in HIV positive individuals than in controls （P 〈 0.05）. Male subjects in the age bracket 18-30 years had significantly higher prevalence when compared to other groups （P 〈 0.05）. Although the present findings revealed a low prevalence of T. gondii antibodies in HIV infection, this suggests that a differential toxoplasmosis diagnosis is also necessary in cases of encephalitis in HIV infection.展开更多
文摘在丝网印刷碳电极(SPCE)表面修饰石墨烯-壳聚糖(GPCS)复合膜和CeO2-Au纳米粒子,利用CeO2-Au纳米粒子对弓形虫特异性抗原(Tg-Ag)的固定,构建了用于弓形虫IgM抗体(Tg-IgM)检测的一次性电流型免疫传感器.采用扫描电镜(SEM)和透射电镜(TEM)对该免疫传感器的修饰进行表征,利用循环伏安法(CV)、交流阻抗法(EIS)和差分脉冲伏安法(DPV)进行电化学性能检测.响应电流与Tg-IgM的浓度在7.5×10-4～24 AU mL-1的范围内呈线性相关,检测限为4.4×10-4AU mL-1.该免疫传感器具有良好的灵敏度、特异性、稳定性和重复性.与ELISA方法相比,该方法结果可靠,孵育时间短,可用于临床上Tg-IgM的检测.
文摘Several researchers have investigated the association of numerous opportunistic pathogens with HIV, little is documented on its association with T. gondii in our environment. We investigated the prevalence of T. gondii immunoglobulins G and M （IgG and IgM） in HIV positive individuals in relation to their cluster of differentiation 4 （CD4） cells count. IgG, IgM and CD4 were assayed using enzyme immunoassay （EIA） and flowcytometry respectively. 341 HIV positive individuals were studied in the present research, 30 （8.7%） of them had T. gondii IgG and IgM, 297 subjects had CD4 cells count a range of 200-400 cells/μL, 27 （9.7%） and 2 （0.6%） of which had T. gondii IgG and IgM respectively. Of the 44 HIV positive subjects with CD4 〉 400 cells/μL, one （2.2%） was positive for T. gondii IgG. In the control group, all the 177 had CD4 〉 400 cells/μL of which, one （0.5%） had T. gondii IgG. The prevalence of T. gondii infection was significantly higher in HIV positive individuals than in controls （P 〈 0.05）. Male subjects in the age bracket 18-30 years had significantly higher prevalence when compared to other groups （P 〈 0.05）. Although the present findings revealed a low prevalence of T. gondii antibodies in HIV infection, this suggests that a differential toxoplasmosis diagnosis is also necessary in cases of encephalitis in HIV infection.
