期刊文献+
共找到6篇文章
< 1 >
每页显示 20 50 100
TaqMan PCR定量检测人类体液中的弓形虫DNA
1
作者 郭凯文 《国外医学(寄生虫病分册)》 2002年第2期86-87,共2页
该研究建立一种快速、灵敏的方法,通过使用弓形虫特异性荧光猝灭探针,以real-time PCR法来检测和定量弓形虫模板DNA。此探针是通过引物设计软件设计出的一段靶基因反义链特异性的序列,含有35个核苷酸。其原理是:探针首先与弓形虫特异性... 该研究建立一种快速、灵敏的方法,通过使用弓形虫特异性荧光猝灭探针,以real-time PCR法来检测和定量弓形虫模板DNA。此探针是通过引物设计软件设计出的一段靶基因反义链特异性的序列,含有35个核苷酸。其原理是:探针首先与弓形虫特异性引物扩增出的靶序列进行内杂交。 展开更多
关键词 弓形虫 诊断 TaqManPCR定量检测 体液 弓形虫dna
下载PDF
艾滋病患者合并弓形虫感染状况研究 被引量:13
2
作者 李晓灵 曹玲 +3 位作者 余昌秀 何盛华 蔡琳 周锐锋 《四川医学》 CAS 2012年第4期678-680,共3页
目的了解艾滋病患者合并弓形虫感染状况及其对艾滋病疾病进展的影响。方法以278例在我院住院的艾滋病确诊患者为研究对象,采集外周血,分离血浆,通过弓形虫核酸扩增(PCR)实验检测弓形虫DNA。同时检测CD+4T淋巴细胞和血浆病毒载量的水平... 目的了解艾滋病患者合并弓形虫感染状况及其对艾滋病疾病进展的影响。方法以278例在我院住院的艾滋病确诊患者为研究对象,采集外周血,分离血浆,通过弓形虫核酸扩增(PCR)实验检测弓形虫DNA。同时检测CD+4T淋巴细胞和血浆病毒载量的水平。结果在278例艾滋病患者中合并弓形虫感染者8例,感染率为2.88%,其中87.5%的患者主要存在于CD+4T淋巴细胞低于50/μl的艾滋病感染者中。合并弓形虫感染者的CD+4T淋巴细胞低于HIV单一感染者,而病毒载量则呈相反趋势。结论对艾滋病患者的管理,在监测CD+4T淋巴细胞的同时,应加强监测弓形虫的混合感染,可结合CD+4T淋巴细胞计数对患者进行预防性服药,降低混合感染造成的死亡。 展开更多
关键词 艾滋病 弓形虫dna 混合感染
下载PDF
弓形虫感染与早期先兆流产的关系
3
作者 廖丹梅 《广西医学》 CAS 2006年第11期1800-1801,共2页
关键词 早期先兆流产 弓形虫感染 先天性弓形虫 胎儿多发畸形 新生儿发育 弓形虫dna 胞内寄生 临床症状
下载PDF
Protective effect of DNA-mediated immunization with a combination of SAG1 and IL-2 gene adjuvant against infection of Toxoplasma gondii in mice 被引量:2
4
作者 陈观今 陈海峰 +2 位作者 郭虹 郑焕钦 汪琦 《Chinese Medical Journal》 SCIE CAS CSCD 2002年第10期1448-1452,共5页
OBJECTIVE: To characterize the immune response induced by SAG1 encoding plasmid combined with IL-2 gene adjuvant in mice and to assess the protective effect of this vaccination against toxoplasmosis. METHODS: Mice wer... OBJECTIVE: To characterize the immune response induced by SAG1 encoding plasmid combined with IL-2 gene adjuvant in mice and to assess the protective effect of this vaccination against toxoplasmosis. METHODS: Mice were co-injected intramuscularly with plasmid encoding Toxoplasma gondii SAG1 plus murine IL-2 expression vector at a dose of 100 microg. Booster immunizations were employed 2 more times at 3-week interval. As controls, mice were inoculated with PBS or empty plasmid pcDNA3. Humoral and cellular responses were assayed using ELISA for the determination of Ab, Ab isotype and IFN-gamma, as well as IL-4. To detect the integration and dissemination of DNA in the injected mice, PCR and in situ hybridization were performed. All mice were then infected with highly virulent RH tachyzoites of Toxoplasma gondii intraperitoneally. RESULTS: Significant increases in specific IgG levels were observed in mice after immunization three times with SAG1 expression plasmid. With respect to the IgG isotype, co-inoculation of IL-2 expression plasmid enhanced the level of IgG2a and the production of IFN-gamma. Challenging mice by vaccinating with combined plasmids with RH tachyzoites resulted in prolonged survival. CONCLUSION: Humoral and cytokine responses elicited by SAG1 DNA immunization can be modulated by co-inoculation with IL-2 expression plasmid. The use of DNA vaccine in combination with an appropriate cytokine gene to prevent T. gondii infection warrants further investigation. 展开更多
关键词 Antigens Protozoan Animals Antibodies Protozoan Cytokines Female IMMUNIZATION Immunoglobulin G Interleukin-2 MICE Protozoan Proteins Protozoan Vaccines Research Support Non-U.S. Gov't TOXOPLASMA Toxoplasmosis Animal Vaccines dna
原文传递
Immunity induced by DNA vaccine of plasmid encoding the rhoptry protein 1 gene combined with the genetic adjuvant of pcIFN-γ against Toxoplasma gondii in mice 被引量:22
5
作者 郭虹 陈观今 +2 位作者 吕芳丽 陈海峰 郑焕钦 《Chinese Medical Journal》 SCIE CAS CSCD 2001年第3期93-96,111,共5页
Objective To construct the eukaryotic expression recombinant plasmid, pcIFN γ, as a genetic adjuvant and observe the immune responses elicited by pcDNA3 rhoptry protein 1 (pc ROP1) combined with pcIFN γ aga... Objective To construct the eukaryotic expression recombinant plasmid, pcIFN γ, as a genetic adjuvant and observe the immune responses elicited by pcDNA3 rhoptry protein 1 (pc ROP1) combined with pcIFN γ against Toxoplasma gondii (T gondii) infection in mice Methods A fragment of the IFN γ gene was directly inserted into the pcDNA3 plasmid and identified by two restriction endonucleases digestion pcIFN and pcROP1 DNA was injected into the left leg muscle of mice at a dosage of 100?μg, and a booster vaccination was given at the same dosage after two weeks Control groups were injected with pcDNA3 blank plasmid or normal saline At 30, 50 and 70 days after booster injection, kinetic tests were carried out: MTT assay for the proliferation response of T lymphocyte cells and the activity of NK cells, sandwich ABC ELISA for the determination of IFN γ, IL 2 and IL 10; a serum enzymetic aassay for nitric oxide (NO) in sera and ELISA for the titer of IgG antibody in sera Results The recombinant plasmid, pcIFN γ was constructed The proliferation response of spleen T lymph cells, NK cell killing activity, and serum levels of IFN γ, IL 2 and NO in mice injected with pcROP1 and pcIFN γ were higher than in those injected with pcROP1 alone There was no difference in IgG antibody levels between the two groups Conclusion The genetic adjuvant, pcIFN γ, could enhance the cellular immune response induced by DNA vaccine of pcROP1 in mice against Toxoplasma gondii infection 展开更多
关键词 Toxoplasma gondii · rhoptry protein 1 · dna vaccination · genetic adjuvant · pcIFN γ
原文传递
Protective effect of DNA-mediated immunization with a combination of SAG1 and IL-2 gene adjuvant against infection of Toxoplasma gondii in mice
6
作者 陈观今 陈海峰 +1 位作者 郭虹 郑焕钦 《Chinese Medical Journal》 SCIE CAS CSCD 2002年第10期8-12,142,共6页
To characterize the immune response induced by SAG1 encoding plasmid combined with IL 2 gene adjuvant in mice and to assess the protective effect of this vaccination against toxoplasmosis Methods Mice were co inje... To characterize the immune response induced by SAG1 encoding plasmid combined with IL 2 gene adjuvant in mice and to assess the protective effect of this vaccination against toxoplasmosis Methods Mice were co injected intramuscularly with plasmid encoding Toxoplasma gondii SAG1 plus murine IL 2 expression vector at a dose of 100 μg Booster immunizations were employed 2 more times at 3 week interval As controls, mice were inoculated with PBS or empty plasmid pcDNA3 Humoral and cellular responses were assayed using ELISA for the determination of Ab, Ab isotype and IFN γ, as well as IL 4 To detect the integration and dissemination of DNA in the injected mice, PCR and in situ hybridization were performed All mice were then infected with highly virulent RH tachyzoites of Toxoplasma gondii intraperitoneally Results Significant increases in specific IgG levels were observed in mice after immunization three times with SAG1 expression plasmid With respect to the IgG isotype, co inoculation of IL 2 expression plasmid enhanced the level of IgG2a and the production of IFN γ Challenging mice by vaccinating with combined plasmids with RH tachyzoites resulted in prolonged survival Conclusion Humoral and cytokine responses elicited by SAG1 DNA immunization can be modulated by co inoculation with IL 2 expression plasmid The use of DNA vaccine in combination with an appropriate cytokine gene to prevent T gondii infection warrants further investigation 展开更多
关键词 dna immunization · Toxoplasma gondi · surface antigen 1 (SAG1) · gene adjuvant · IL 2
全文增补中
上一页 1 下一页 到第
使用帮助 返回顶部