Toxoplasma gondii is a worldwide spread protozoan and is able to infect almost all warm-blood animals. No effective drugs are available clinically on toxoplasmosis. Chinese traditional herbal medicines have provided r...Toxoplasma gondii is a worldwide spread protozoan and is able to infect almost all warm-blood animals. No effective drugs are available clinically on toxoplasmosis. Chinese traditional herbal medicines have provided remedies for many health problems. There exists a possibility that Chinese herbs may provide protection against T. gondii. This work aims to assess the protective efficacy of combined Chinese herbs against T. gondii. We screened five herbal medicines that have different pharmacological effects and combined them into a prescription according to the traditional Chinese medicine compatibility principle. The drug potential and protective efficacy were evaluated through a mouse model by determining the survival time, the parasite load in blood and tissues, the change of cell proportions in blood and histological detection. The results showed that the survival time of mice in the 500 mg Chinese herbs group and sulfadiazine group was significantly longer than that of the PBS control group. Also the parasite load in blood and tissues of 500 mg Chinese herbs and sulfadiazine groups was significantly lower than that of PBS group at 7 days post infection（dpi）, which was in accordance with the result of histological detection. Monocyte and neutrophil of infected mice were remarkably increased while lymphocyte was dramatically decreased compared to that of blank group at 7 dpi. The results demonstrated that the 500 mg dosage of our Chinese herbs could slow down the replication of T. gondii and prolong the survival time of mice and could be considered as possible candidate drug against toxoplasmosis.展开更多
Object: To show that increased and invigorated movement of qi and bloodwithin the organism and their concentration in the diseased organ is crucial for acupuncture andlimitations for acupuncture based upon Western med...Object: To show that increased and invigorated movement of qi and bloodwithin the organism and their concentration in the diseased organ is crucial for acupuncture andlimitations for acupuncture based upon Western medicine are not necessarily adequate. Methods:Symptom-related and chronoacupuncture were used within a five stages acupuncture beginning withneedling of competent pair of confluent points and followed by Zi Wu Liu Zhu chronoacupuncture.Results: After 58 symptom-related acupuncture treatments the vision increased from 10 to 70 percentand remained stable. 18 months later one Zi Wu Liu Zhu chronoacupuncture was carried out following aneedling concept of five stages. Within one hour the vision increased from 70 to 90 percent.Conclusions: Ophthalmologists should offer acupuncture to patients suffering from eye in-flammationsand retina scars. Acupuncturists should use Zi Wu Liu Zhu and powerful acupoints and acupointcombinations in order to invigorate and to move qi and blood strongly. This case shows theeffectiveness of acupuncture and chronoacupuncture in a case of retina scar caused by toxoplasmosis.Therefore, acupuncture treatment might be successfully applied in diseases where Western medicinecannot offer help.展开更多
基金supported by the Science and Technology Department of Zhejiang Province(No.2012C12009-2),China
文摘Toxoplasma gondii is a worldwide spread protozoan and is able to infect almost all warm-blood animals. No effective drugs are available clinically on toxoplasmosis. Chinese traditional herbal medicines have provided remedies for many health problems. There exists a possibility that Chinese herbs may provide protection against T. gondii. This work aims to assess the protective efficacy of combined Chinese herbs against T. gondii. We screened five herbal medicines that have different pharmacological effects and combined them into a prescription according to the traditional Chinese medicine compatibility principle. The drug potential and protective efficacy were evaluated through a mouse model by determining the survival time, the parasite load in blood and tissues, the change of cell proportions in blood and histological detection. The results showed that the survival time of mice in the 500 mg Chinese herbs group and sulfadiazine group was significantly longer than that of the PBS control group. Also the parasite load in blood and tissues of 500 mg Chinese herbs and sulfadiazine groups was significantly lower than that of PBS group at 7 days post infection（dpi）, which was in accordance with the result of histological detection. Monocyte and neutrophil of infected mice were remarkably increased while lymphocyte was dramatically decreased compared to that of blank group at 7 dpi. The results demonstrated that the 500 mg dosage of our Chinese herbs could slow down the replication of T. gondii and prolong the survival time of mice and could be considered as possible candidate drug against toxoplasmosis.
文摘Object: To show that increased and invigorated movement of qi and bloodwithin the organism and their concentration in the diseased organ is crucial for acupuncture andlimitations for acupuncture based upon Western medicine are not necessarily adequate. Methods:Symptom-related and chronoacupuncture were used within a five stages acupuncture beginning withneedling of competent pair of confluent points and followed by Zi Wu Liu Zhu chronoacupuncture.Results: After 58 symptom-related acupuncture treatments the vision increased from 10 to 70 percentand remained stable. 18 months later one Zi Wu Liu Zhu chronoacupuncture was carried out following aneedling concept of five stages. Within one hour the vision increased from 70 to 90 percent.Conclusions: Ophthalmologists should offer acupuncture to patients suffering from eye in-flammationsand retina scars. Acupuncturists should use Zi Wu Liu Zhu and powerful acupoints and acupointcombinations in order to invigorate and to move qi and blood strongly. This case shows theeffectiveness of acupuncture and chronoacupuncture in a case of retina scar caused by toxoplasmosis.Therefore, acupuncture treatment might be successfully applied in diseases where Western medicinecannot offer help.
