Objective：To investigate the mechanism of the reversal effect of acupuncture on the fall of blood pressure induced by angiotensin （1-7）[Ang-（1 7）] in the caudal ventrolateral medulla （CVLM） of rats. Methods： A...Objective：To investigate the mechanism of the reversal effect of acupuncture on the fall of blood pressure induced by angiotensin （1-7）[Ang-（1 7）] in the caudal ventrolateral medulla （CVLM） of rats. Methods： Ang-（1-7） and its selective receptor antagonist （DAla7）, Ang（1-7） and Ang（779） were microinjected into the CVLM respectively to induce the change of blood pressure, during which, the concentrations of amino acid neurotransmitters were detected by means of microdialysis and high performance liquid chromatography （HPLC）combined with fluorescent detector, on the other hand, the effects of electro-acupuncture （EA） on the level of blood pressure and the concentrations of amino acid neurotransmitters were also observed. Results： Unilateral microinjection of Ang（1 7） into the CVLM could decrease the blood pressure （BP）, which was accompanied by an increase in the release of Glu and a decrease in the release of Tau in the same site; Whereas microinjection of Ang-779 into the CVLM could elevate BP, which was accompanied by a decrease in the release of Glu and an increase in the release of Tau; EA at acupoint Zusanli （ST 36） for 20 min could inhibit the fall of blood pressure induced by microinjection of Ang-（1-7） into the CVLM and the elevation of blood pressure induced by microinjection of Ang-779 into the CVLM, and in the meantime, it could inhibit the changes of the release of Glu and Tau induced by microinjection of Ang-（1-7） and Ang-779 into the CVLM. Conclusion： The reversal effect of EA on the fall or elevation of blood pressure induced by microinjection of Ang-（1-7） or Ang-779 into the CVLM might be related to the changes of Glu and Tau release.展开更多
The brainstem is a major site in the central nervous system involved in the processing of the cardiovascular reflexes such as the baroreflex and the peripheral chemoreflex. The nucleus tractus solitarius and the rostr...The brainstem is a major site in the central nervous system involved in the processing of the cardiovascular reflexes such as the baroreflex and the peripheral chemoreflex. The nucleus tractus solitarius and the rostral ventrolateral medulla are 2 important brainstem nuclei, and they play pivotal roles in autonomic cardiovascular regulation. Angiotensin II is one of the neurotransmitters involved in the processing of the known that one of the mechanisms by which angiotensin II exerts cardiovascular reflexes within the brainstem. It is well- its effect is via the activation of pathways that generate reactive oxygen species （ROS）. In the central nervous system, ROS are reported to be involved in several pathological dis- eases such as hypertension, heart failure and sleep apnea. However, little is known about the role of ROS in the processing of the cardiovascular reflexes within the brainstem. The present review mainly discussed some recent findings documenting a role for ROS in the processing of the baroreflex and the peripheral chemoreflex in the brainstem.展开更多
基金Supported by the National Nature Science Foundation(30340071)
文摘Objective：To investigate the mechanism of the reversal effect of acupuncture on the fall of blood pressure induced by angiotensin （1-7）[Ang-（1 7）] in the caudal ventrolateral medulla （CVLM） of rats. Methods： Ang-（1-7） and its selective receptor antagonist （DAla7）, Ang（1-7） and Ang（779） were microinjected into the CVLM respectively to induce the change of blood pressure, during which, the concentrations of amino acid neurotransmitters were detected by means of microdialysis and high performance liquid chromatography （HPLC）combined with fluorescent detector, on the other hand, the effects of electro-acupuncture （EA） on the level of blood pressure and the concentrations of amino acid neurotransmitters were also observed. Results： Unilateral microinjection of Ang（1 7） into the CVLM could decrease the blood pressure （BP）, which was accompanied by an increase in the release of Glu and a decrease in the release of Tau in the same site; Whereas microinjection of Ang-779 into the CVLM could elevate BP, which was accompanied by a decrease in the release of Glu and an increase in the release of Tau; EA at acupoint Zusanli （ST 36） for 20 min could inhibit the fall of blood pressure induced by microinjection of Ang-（1-7） into the CVLM and the elevation of blood pressure induced by microinjection of Ang-779 into the CVLM, and in the meantime, it could inhibit the changes of the release of Glu and Tau induced by microinjection of Ang-（1-7） and Ang-779 into the CVLM. Conclusion： The reversal effect of EA on the fall or elevation of blood pressure induced by microinjection of Ang-（1-7） or Ang-779 into the CVLM might be related to the changes of Glu and Tau release.
文摘The brainstem is a major site in the central nervous system involved in the processing of the cardiovascular reflexes such as the baroreflex and the peripheral chemoreflex. The nucleus tractus solitarius and the rostral ventrolateral medulla are 2 important brainstem nuclei, and they play pivotal roles in autonomic cardiovascular regulation. Angiotensin II is one of the neurotransmitters involved in the processing of the known that one of the mechanisms by which angiotensin II exerts cardiovascular reflexes within the brainstem. It is well- its effect is via the activation of pathways that generate reactive oxygen species （ROS）. In the central nervous system, ROS are reported to be involved in several pathological dis- eases such as hypertension, heart failure and sleep apnea. However, little is known about the role of ROS in the processing of the cardiovascular reflexes within the brainstem. The present review mainly discussed some recent findings documenting a role for ROS in the processing of the baroreflex and the peripheral chemoreflex in the brainstem.
