栗疫菌弱毒力特征的传递及其影响因素

dsRNA in Cryphonectria parasitica could be transmitted to progeny through conidia with varying efficiency in culture.Both light and prolonging cultural time could reduce the transmission efficiency,but the effect of light was more efficient.No dsRNA segments were found to be loss after 30 generations subculture,indicating that dsRNA could transmitted stably through subculture.Vegetative incompatibility was a barrier for the transfer of dsRNA from one isolate to another,and hypovirulence conversion reduced as the number of different VC genes between donor and recipient isolates increased.

栗疫病菌dsRNA的传递及其影响因素

对ｄｓＲＮＡ通过分生孢子、继代培养和菌丝融合传递的效率及其影响因素进行了研究。ｄｓＲＮＡ可通过分生孢子传递，但效果不稳定；提供光照和延长培养时间均可降低其传递效率，光照的不利影响更为明显；继代培养３０代未发现ｄｓＲＮＡ片段丢失，表明ｄｓＲＮＡ通过继代培养可稳定传递；菌丝融合传递ｄｓＲＮＡ受到营养体不亲和性的限制，但不亲和菌株间仍可获得一定的转化率，有的高达１００％；本研究使用两步转化的方法，明显地提高了弱毒力的转化率。