在司法鉴定及与之相关的商品检验工作中,有时需要测定红色墨水中的染料组成。有些红色墨水中的红色成分由酸性大红3R 和弱酸红 A 两种染料混合配制而成,这两种染料的吸收光谱重叠,相互干扰。本文建立了多波长线性回归分光光度法同时测...在司法鉴定及与之相关的商品检验工作中,有时需要测定红色墨水中的染料组成。有些红色墨水中的红色成分由酸性大红3R 和弱酸红 A 两种染料混合配制而成,这两种染料的吸收光谱重叠,相互干扰。本文建立了多波长线性回归分光光度法同时测定红墨水中酸性大红3R 和弱酸红 A 的方法,可消除酸性大红3R 和弱酸红 A 的相互干扰,同时测定两种染料的浓度。实验测定了酸性大红3R 和弱酸红 A 在400-600nm 波长范围的吸收光谱;考察了两染料的吸光度的线性范围,进行了两染料混合标准液的吸光度加合性实验,结果表明,在450～580nm 波长区间和0-40mg/L 浓度范围内,两种染料均服从 Beer定律(r>0.996),具有良好的线性和吸光度加合性。实验选择了在波长500-530nm 区间每隔2nm 的16个波长作为测定和回归波长。考察了方法的精密度和准确度,结果测得酸性大红3R 的回收率为94.0～104.5%,RSD 为2.4～4.3%;弱酸红 A 的回收率为98.0～104.0%,RSD 为2.1～4.6%。本方法测定了红墨水样品中酸性大红3R 和弱酸红 A 的配比,结果令人满意。展开更多
[TiO2],t and pH are named as the independent variables and the percentage of decolor(DC%)as the dependent variable.A Box-Behnken design and U12(6×4×3)design with three factors were established to form the tr...[TiO2],t and pH are named as the independent variables and the percentage of decolor(DC%)as the dependent variable.A Box-Behnken design and U12(6×4×3)design with three factors were established to form the training set and the validation set,respectively.And back propagation neural network(BPNN)model is adopted in order to establish the model of photo-catalytic degradation about weak acidic dyes mixture of the GRS big red and weak acid red R.The best result shows that the correlation coefficient(R)is 0.9345 and the mean relative error between the predictive value and experimental value(MRE(%))is 3.23,for training set;the value of R is 0.9257,MRE(%)is 2.75,for the validation set.Besides,we discussed the influences of the pH,[TiO2],and t vs.DC% by the BPNN model.The optimized experimental condition obtained is pH=5.0,[TiO2]=1.50g/L,and t=40min based on the BPNN model,and combination with optimization of nonlinear constraints programming.The experimental value of DC is 99.23%,the predictive value is 98.98%,and the relative error is-0.25% between the predictive value and the experimental value,in the optimized experimental condition.Above all these indicate that the model can not only simulate the system of photo-catalytic degradation commendably but also can obtain the optimal experimental conditions.展开更多
In weak acidic medium the alkaline cationic Safranine T interacts with acidic biological rnacromolecule heparin intensively causing the change of the molecular conformation, the maximum absorption wavelength and the a...In weak acidic medium the alkaline cationic Safranine T interacts with acidic biological rnacromolecule heparin intensively causing the change of the molecular conformation, the maximum absorption wavelength and the absorption value. The optimum experimental condition was given and a new method to determine the heparin was established. The linear range was 0.05-2.0 mg/L and the correlation coefficient was 0.9974. The method has been applied to analyze the samples and the results are satisfactory. Furthermore, the interaction mechanism was indicated.展开更多
文摘在司法鉴定及与之相关的商品检验工作中,有时需要测定红色墨水中的染料组成。有些红色墨水中的红色成分由酸性大红3R 和弱酸红 A 两种染料混合配制而成,这两种染料的吸收光谱重叠,相互干扰。本文建立了多波长线性回归分光光度法同时测定红墨水中酸性大红3R 和弱酸红 A 的方法,可消除酸性大红3R 和弱酸红 A 的相互干扰,同时测定两种染料的浓度。实验测定了酸性大红3R 和弱酸红 A 在400-600nm 波长范围的吸收光谱;考察了两染料的吸光度的线性范围,进行了两染料混合标准液的吸光度加合性实验,结果表明,在450～580nm 波长区间和0-40mg/L 浓度范围内,两种染料均服从 Beer定律(r>0.996),具有良好的线性和吸光度加合性。实验选择了在波长500-530nm 区间每隔2nm 的16个波长作为测定和回归波长。考察了方法的精密度和准确度,结果测得酸性大红3R 的回收率为94.0～104.5%,RSD 为2.4～4.3%;弱酸红 A 的回收率为98.0～104.0%,RSD 为2.1～4.6%。本方法测定了红墨水样品中酸性大红3R 和弱酸红 A 的配比,结果令人满意。
文摘[TiO2],t and pH are named as the independent variables and the percentage of decolor(DC%)as the dependent variable.A Box-Behnken design and U12(6×4×3)design with three factors were established to form the training set and the validation set,respectively.And back propagation neural network(BPNN)model is adopted in order to establish the model of photo-catalytic degradation about weak acidic dyes mixture of the GRS big red and weak acid red R.The best result shows that the correlation coefficient(R)is 0.9345 and the mean relative error between the predictive value and experimental value(MRE(%))is 3.23,for training set;the value of R is 0.9257,MRE(%)is 2.75,for the validation set.Besides,we discussed the influences of the pH,[TiO2],and t vs.DC% by the BPNN model.The optimized experimental condition obtained is pH=5.0,[TiO2]=1.50g/L,and t=40min based on the BPNN model,and combination with optimization of nonlinear constraints programming.The experimental value of DC is 99.23%,the predictive value is 98.98%,and the relative error is-0.25% between the predictive value and the experimental value,in the optimized experimental condition.Above all these indicate that the model can not only simulate the system of photo-catalytic degradation commendably but also can obtain the optimal experimental conditions.
文摘In weak acidic medium the alkaline cationic Safranine T interacts with acidic biological rnacromolecule heparin intensively causing the change of the molecular conformation, the maximum absorption wavelength and the absorption value. The optimum experimental condition was given and a new method to determine the heparin was established. The linear range was 0.05-2.0 mg/L and the correlation coefficient was 0.9974. The method has been applied to analyze the samples and the results are satisfactory. Furthermore, the interaction mechanism was indicated.
