Micromolding in capillaries of a micro square array was carried out for polystyrene solution in acetone by means of swollen polydimethylsiloxane （PDMS） elastomeric stamp. The resulting micro-cubic poles were isolate...Micromolding in capillaries of a micro square array was carried out for polystyrene solution in acetone by means of swollen polydimethylsiloxane （PDMS） elastomeric stamp. The resulting micro-cubic poles were isolated and separable when the added amount of the polystyrene solution was small. Some special distorting micro-patterns in the micro-square array were observed because of shrinkage resulting from the varying evaporation rate of solvent at different places.展开更多
Ultra thin epitaxial CoSi 2 films are fabricated by solid state reaction of a deposited bilayer of Co(3nm)/Ti (1nm) on n Si(100) substrates at different temperatures.The local barrier heights of the CoSi 2/Si cont...Ultra thin epitaxial CoSi 2 films are fabricated by solid state reaction of a deposited bilayer of Co(3nm)/Ti (1nm) on n Si(100) substrates at different temperatures.The local barrier heights of the CoSi 2/Si contacts are determined by using the ballistic electron emission microscopy (BEEM) and its spectroscopy (BEES) at low temperature.For CoSi 2/Si contact annealed at 800℃,the spatial distribution of barrier heights,which have mean barrier height of 599meV and a standard deviation of 21meV,obeys the Gaussian Function.However,for a sample that is annealed at 700℃,the barrier heights of it are more inhomogenous.Its local barrier heights range from 152meV to 870meV,which implies the large inhomogeneity of the CoSi 2 film.展开更多
Considering the delay effect of initial lining and revising the Winkler elastic foundation model,an analytical approach based on Pasternak elastic foundation beam theory for pipe roof reinforcement was put forward. Wi...Considering the delay effect of initial lining and revising the Winkler elastic foundation model,an analytical approach based on Pasternak elastic foundation beam theory for pipe roof reinforcement was put forward. With the example of a certain tunnel excavation,the comparison of the values of longitudinal strain of reinforcing pipe between field monitoring and analytical approach was made. The results indicate that Pasternak model,which considers a more realistic hypothesis in the elastic soil than Winkler model,gives more accurate calculation and agrees better with the result of field monitoring. The difference of calculation results between these two models is about 7%,and Pasternak model is proved to be a better way to study the reinforcement mechanism and improve design practice. The calculation results also reveal that the reinforcing pipes act as levers,which increases longitudinal load transfer to an unexcavated area,and consequently decreases deformation and increases face stability.展开更多
The resilient modulus(MR)of subgrade soils is usually used to characterize the stiffness of subgrade and is a crucial parameter in pavement design.In order to determine the resilient modulus of compacted subgrade soil...The resilient modulus(MR)of subgrade soils is usually used to characterize the stiffness of subgrade and is a crucial parameter in pavement design.In order to determine the resilient modulus of compacted subgrade soils quickly and accurately,an optimized artificial neural network(ANN)approach based on the multi-population genetic algorithm(MPGA)was proposed in this study.The MPGA overcomes the problems of the traditional ANN such as low efficiency,local optimum and over-fitting.The developed optimized ANN method consists of ten input variables,twenty-one hidden neurons,and one output variable.The physical properties(liquid limit,plastic limit,plasticity index,0.075 mm passing percentage,maximum dry density,optimum moisture content),state variables(degree of compaction,moisture content)and stress variables(confining pressure,deviatoric stress)of subgrade soils were selected as input variables.The MR was directly used as the output variable.Then,adopting a large amount of experimental data from existing literature,the developed optimized ANN method was compared with the existing representative estimation methods.The results show that the developed optimized ANN method has the advantages of fast speed,strong generalization ability and good accuracy in MR estimation.展开更多
It is critical for the material to be of active supporting capacity before initial collapse ot mare root wltn supermgn water material backfill mining, and the maximum bending moment should be first calculated in order...It is critical for the material to be of active supporting capacity before initial collapse ot mare root wltn supermgn water material backfill mining, and the maximum bending moment should be first calculated in order to determine the initial collapse span. In the light of principal of virtual work, the simple expression of deflection, bending moment of elastic clamped plate were deduced under the condition of vertical uniform distributed load, horizontal pressure and supporting by elastic foundation, and then, the maximal bending moment expression was derived too. At the same time, the influence degree on square clamped plate by adding horizontal pressure and elastic foundation were analyzed. The results show that the effect of horizontal pressure on maximal bending moment can be ignored when the value of horizontal pressure is two orders of magni- tude less than that of coeificient of elastic stiffness existing elastic foundation.展开更多
A protocol is presented for genetically engineering loblolly pine (Pinus taeda L.) using particle bombardment. This protocol enabled the routine transformation of loblolly pine plants that were previously difficult to...A protocol is presented for genetically engineering loblolly pine (Pinus taeda L.) using particle bombardment. This protocol enabled the routine transformation of loblolly pine plants that were previously difficult to transform. Mature zygotic embryos were used to be bombarded and to generate organogenic callus and transgenic regenerated plants. Plasmid pB48.215 DNA contained a synthetic Bacillus thuringiensis (B.t.) cryIAc coding sequence flanked by the double cauliflower mosaic virus (CaMV) 35S promoter and nopaline synthase (Nos) terminator sequences, and the selectable marker gene, neomycin phosphotransferase II (nptII) controlled by the promoter of the nopaline synthase gene was introduced into loblolly pine tissues by particle bombardment. The transformed tissues were proliferated and selected by kanamycin resistance conferred by the introduced NPTII gene. Shoot regeneration was induced from the kanamycin-resistant callus, and transgenic plantlets were then produced. The presence of the introduced genes in the transgenic loblolly pine plants was confirmed by polymerase chain reactions (PCR) analysis, by Southern blot analysis, and insect feeding assays. The recovered transgenic plants were acclimatized and then established in soil.展开更多
基金supported by the National Natural Science Foundation of China(No.20374049)the Specialized Research Pund for the Doctoral Program of Higher Education(20040358018).
文摘Micromolding in capillaries of a micro square array was carried out for polystyrene solution in acetone by means of swollen polydimethylsiloxane （PDMS） elastomeric stamp. The resulting micro-cubic poles were isolated and separable when the added amount of the polystyrene solution was small. Some special distorting micro-patterns in the micro-square array were observed because of shrinkage resulting from the varying evaporation rate of solvent at different places.
文摘Ultra thin epitaxial CoSi 2 films are fabricated by solid state reaction of a deposited bilayer of Co(3nm)/Ti (1nm) on n Si(100) substrates at different temperatures.The local barrier heights of the CoSi 2/Si contacts are determined by using the ballistic electron emission microscopy (BEEM) and its spectroscopy (BEES) at low temperature.For CoSi 2/Si contact annealed at 800℃,the spatial distribution of barrier heights,which have mean barrier height of 599meV and a standard deviation of 21meV,obeys the Gaussian Function.However,for a sample that is annealed at 700℃,the barrier heights of it are more inhomogenous.Its local barrier heights range from 152meV to 870meV,which implies the large inhomogeneity of the CoSi 2 film.
基金Project(IRT0518) supported by the Program of Innovative Research Team of Ministry of Education of China
文摘Considering the delay effect of initial lining and revising the Winkler elastic foundation model,an analytical approach based on Pasternak elastic foundation beam theory for pipe roof reinforcement was put forward. With the example of a certain tunnel excavation,the comparison of the values of longitudinal strain of reinforcing pipe between field monitoring and analytical approach was made. The results indicate that Pasternak model,which considers a more realistic hypothesis in the elastic soil than Winkler model,gives more accurate calculation and agrees better with the result of field monitoring. The difference of calculation results between these two models is about 7%,and Pasternak model is proved to be a better way to study the reinforcement mechanism and improve design practice. The calculation results also reveal that the reinforcing pipes act as levers,which increases longitudinal load transfer to an unexcavated area,and consequently decreases deformation and increases face stability.
基金Project(51878078)supported by the National Natural Science Foundation of ChinaProject(2018-025)supported by the Training Program for High-level Technical Personnel in Transportation Industry,ChinaProject(CTKY-PTRC-2018-003)supported by the Design Theory,Method and Demonstration of Durability Asphalt Pavement Based on Heavy-duty Traffic Conditions in Shanghai Area,China。
文摘The resilient modulus(MR)of subgrade soils is usually used to characterize the stiffness of subgrade and is a crucial parameter in pavement design.In order to determine the resilient modulus of compacted subgrade soils quickly and accurately,an optimized artificial neural network(ANN)approach based on the multi-population genetic algorithm(MPGA)was proposed in this study.The MPGA overcomes the problems of the traditional ANN such as low efficiency,local optimum and over-fitting.The developed optimized ANN method consists of ten input variables,twenty-one hidden neurons,and one output variable.The physical properties(liquid limit,plastic limit,plasticity index,0.075 mm passing percentage,maximum dry density,optimum moisture content),state variables(degree of compaction,moisture content)and stress variables(confining pressure,deviatoric stress)of subgrade soils were selected as input variables.The MR was directly used as the output variable.Then,adopting a large amount of experimental data from existing literature,the developed optimized ANN method was compared with the existing representative estimation methods.The results show that the developed optimized ANN method has the advantages of fast speed,strong generalization ability and good accuracy in MR estimation.
基金Supported by the National Natural Science Foundation of China （41071273） the Special Research Fund for the Doctoral Program of Higher Education of China （200090095110002）
文摘It is critical for the material to be of active supporting capacity before initial collapse ot mare root wltn supermgn water material backfill mining, and the maximum bending moment should be first calculated in order to determine the initial collapse span. In the light of principal of virtual work, the simple expression of deflection, bending moment of elastic clamped plate were deduced under the condition of vertical uniform distributed load, horizontal pressure and supporting by elastic foundation, and then, the maximal bending moment expression was derived too. At the same time, the influence degree on square clamped plate by adding horizontal pressure and elastic foundation were analyzed. The results show that the effect of horizontal pressure on maximal bending moment can be ignored when the value of horizontal pressure is two orders of magni- tude less than that of coeificient of elastic stiffness existing elastic foundation.
文摘A protocol is presented for genetically engineering loblolly pine (Pinus taeda L.) using particle bombardment. This protocol enabled the routine transformation of loblolly pine plants that were previously difficult to transform. Mature zygotic embryos were used to be bombarded and to generate organogenic callus and transgenic regenerated plants. Plasmid pB48.215 DNA contained a synthetic Bacillus thuringiensis (B.t.) cryIAc coding sequence flanked by the double cauliflower mosaic virus (CaMV) 35S promoter and nopaline synthase (Nos) terminator sequences, and the selectable marker gene, neomycin phosphotransferase II (nptII) controlled by the promoter of the nopaline synthase gene was introduced into loblolly pine tissues by particle bombardment. The transformed tissues were proliferated and selected by kanamycin resistance conferred by the introduced NPTII gene. Shoot regeneration was induced from the kanamycin-resistant callus, and transgenic plantlets were then produced. The presence of the introduced genes in the transgenic loblolly pine plants was confirmed by polymerase chain reactions (PCR) analysis, by Southern blot analysis, and insect feeding assays. The recovered transgenic plants were acclimatized and then established in soil.
