A bacterial cell surface display technique based on an ice nucleation protein has been employed for the development of live vaccine against viral infection. Due to its ubiquitous ability to invade host cells, Salmonel...A bacterial cell surface display technique based on an ice nucleation protein has been employed for the development of live vaccine against viral infection. Due to its ubiquitous ability to invade host cells, Salmonella typhimurium might be a good candidate for displaying viral antigens. We demonstrated the surface display of domain III of Japanese encephalitis virus E protein and the enhanced green fluorescent protein on S. typhimurium BRD509 using the ice nucleation protein. The effects of the motif in the ice nucleation protein on the effective display of integral protein were also investigated. The results showed that display motifs in the protein can target integral foreign protein on the surface of S. typhimurium BRD509. Moreover, recombinant strains with surface displayed viral proteins retained their invasiveness, suggesting that the recombinant S. typhimurium can be used as live vaccine vector for eliciting complete immunogenicity. The data may yield better understanding of the mechanism by which ice nucleation protein displays foreign proteins in the Salmonella strain.展开更多
This paper considers experimental situations where the interested effects have to be or- thogonal to a set of nonnegligible effects. It is shown that various types of orthogonal arrays with mixed strength are A-optima...This paper considers experimental situations where the interested effects have to be or- thogonal to a set of nonnegligible effects. It is shown that various types of orthogonal arrays with mixed strength are A-optimal for estimating the parameters in ANOVA high dimension model representation. Both cases including interactions or not are considered in the model. In particularly, the estimations of all main effects are A-optimal in a mixed strength （2, 2）3 orthogonal array and the estimations of all main effects and two-factor interactions in G~ x G~ are A-optimal in a mixed strength （2, 2）4 orthogonal array. The properties are also illustrated through a simulation study.展开更多
基金The Knowledge Innovation Program Key Project (KSCX1-YW-R-07)
文摘A bacterial cell surface display technique based on an ice nucleation protein has been employed for the development of live vaccine against viral infection. Due to its ubiquitous ability to invade host cells, Salmonella typhimurium might be a good candidate for displaying viral antigens. We demonstrated the surface display of domain III of Japanese encephalitis virus E protein and the enhanced green fluorescent protein on S. typhimurium BRD509 using the ice nucleation protein. The effects of the motif in the ice nucleation protein on the effective display of integral protein were also investigated. The results showed that display motifs in the protein can target integral foreign protein on the surface of S. typhimurium BRD509. Moreover, recombinant strains with surface displayed viral proteins retained their invasiveness, suggesting that the recombinant S. typhimurium can be used as live vaccine vector for eliciting complete immunogenicity. The data may yield better understanding of the mechanism by which ice nucleation protein displays foreign proteins in the Salmonella strain.
基金the National Natural Science Foundation of China under Grant Nos.11171065,11301073the Natural Science Foundation of Jiangsu under Grant No.BK20141326+1 种基金the Research Fund for the Doctoral Program of Higher Education of China under Grant No.20120092110021Scientific Research Foundation of Graduate School of Southeast University under Grant No.YBJJ1444
文摘This paper considers experimental situations where the interested effects have to be or- thogonal to a set of nonnegligible effects. It is shown that various types of orthogonal arrays with mixed strength are A-optimal for estimating the parameters in ANOVA high dimension model representation. Both cases including interactions or not are considered in the model. In particularly, the estimations of all main effects are A-optimal in a mixed strength （2, 2）3 orthogonal array and the estimations of all main effects and two-factor interactions in G~ x G~ are A-optimal in a mixed strength （2, 2）4 orthogonal array. The properties are also illustrated through a simulation study.
