当归芍药散对大鼠实验性牙移动后三叉神经脊髓核尾侧亚核区域中c—fos和iba-1表达的影响

【目的】探讨当归芍药散对大鼠实验性牙移动后三叉神经脊髓核尾侧亚核（SpVc）区域中致癌因子cfos和小胶质细胞膜特异性标记物iba1表达的影响。【方法】36只SD大鼠随机分为对照组（Sham组），牙齿加力组（ETM组）和当归芍药散（Danggui-Shaoyao-san ,DSS）处理组（DSS组）；分别检测在加力后不同时间点单位时间空口咀嚼时间（chewing-like jaw movements，VCM）及牙齿移动后1 d各组SpVc内fos和iba-1免疫组化及Western-blot蛋白表达的变化。【结果】ETM组大鼠VCM在加力d1时间点达到峰值，随后逐渐下降；和ETM组比较，DSS组在4 h，12 h开始减少（P〈0.05），牙移动后d1减少最明显（P〈0.01）, ETM组与同一时间点Sham组比较无显著性差异（P〉0.05）。Sham组SpVc有很少量c-fos，iba-1表达，呈阴性或浅染，胞体较小，突起表达弱，呈散在分布，形态不清晰；ETM组免疫阳性细胞表达明显增加，染色加深，胞体变大，形态清晰丰满，且分布区域向SpVc较深层趋向，DSS组SpVc区域仅有很少量c-fos，iba-1表达，免疫阳性小胶质细胞的胞体小，胞质着色较深，突起弱阳性表达。ETM组c-fos，iba-1蛋白表达均显著高于Sham组和DSS组，差异有统计学意义（P〈0.05）；而Sham组与DSS组比较无显著性差异（P〉0.05）。【结论】大鼠实验性后牙移动后局部激活SpVc区域c-fos，iba-1表达，DSS处理后可通过弱化牙齿移动后大鼠SpVc神经元和胶质细胞的激活反应，可减轻大鼠牙齿移动后自发痛。

Chemical Constituents of Angelica sinensis

Aim To investigate the active constituents responsible for thepharmacological activities of Angelica sinensis (Oliv) Diels. Methods Chromatography was used toisolate chemical components, and spectroscopy was used to identify their structures. Results Sevencompounds were isolated and their structures were identified as ferulic acid (1), conife-rylferukte(2) , bis (2-ethylhexyl) phthalate (3), dibutyl phthalate (4), lignoceric acid (5), palmitic acid(6), and Z-6, 7-cis-dihydroxyligustilide (7) Conclusion Bis (2-ethylhexyl) phthalate and dibutylphthalate were obtained from Angelica sinensis for the first time.

Optimizing medication strategies for liver cancer:unraveling the mechanisms of key drug combinations in the comprehensive guide to effective tumor prescriptions

This study delved into the formulaic patterns of liver cancer treatment outlined in“The Complete Book of Good Tumor Prescriptions”and explored the action mechanisms of core traditional Chinese medicine(TCM)combinations.Initially,liver cancer treatment formulas were extracted from the aforementioned book,and Excel 2017 was employed for calculating the frequency,properties,and meridians associated with the TCMs.IBM SPSS Modeler 18.0 was utilized for the analysis of TCM association rules,and Cytoscape 3.7.2 was employed for the visualization of these rules.Subsequently,network pharmacology was utilized to analyze the action mechanisms of core TCM combinations,leveraging TCMSP,TCMID,Genecards,DAVID,and other databases.The results revealed the inclusion of 131 prescriptions encompassing 303 TCMs.The core TCMs predominantly comprised those invigorating blood circulation and removing blood stasis,clearing heat,and tonifying deficiencies.The flavor of the TCMs was chiefly bitter or pungent,with a prevailing cold property entering the liver and spleen meridians.Association analysis indicated that the support of Angelicae sinensis radix-Trionycis carapax was the highest.Network pharmacology predictions indicated that Angelicae sinensis radix-Trionycis carapax possessed 20 target genes associated with anti-liver cancer properties,including IL-6,MAPK3,and SRC.Gene survival analysis demonstrated high expression of MAPK3 and SRC in liver cancer patients,correlating with a poor prognosis.KEGG analysis identified major anti-liver cancer pathways for Angelicae sinensis radix-Trionycis carapax,encompassing the cancer pathway,TNF signaling pathway,and prolactin signaling pathway.In summary,this study elucidated that TCMs for liver cancer treatment primarily consisted of invigorating blood circulation and removing blood stasis,antipyretic,and tonifying medicines.The core TCM pair,Angelicae sinensis radix-Trionycis carapax,appeared pivotal in this context.The underlying mechanism might involve the modulation of key genes,such as MAPK3 and SRC,and the regulation of pathways,including the cancer pathway,TNF signaling pathway,and prolactin signaling pathway.