With 50 large-flowered Chrysanthemum varieties from germplasm nursery of Wunaoshan Forest Farm in Macheng City as research objects, 64 morphological traits were investigated by field experiments adopting randomized bl...With 50 large-flowered Chrysanthemum varieties from germplasm nursery of Wunaoshan Forest Farm in Macheng City as research objects, 64 morphological traits were investigated by field experiments adopting randomized block design. The morphological differences were observed by uniformity analysis, variability analysis, principal component analysis and cluster analysis. The result showed that the vari- able coefficients of 59 traits were greater than 15%; the contribution rate of first seven principal components reached 81.45%; and it was found by clustering analy- sis that the 50 germplasm resources could be divided into four clusters with obvious morphological differences, and plant type could be used as an index for classifica- tion.展开更多
Five hundred samples ofDonax spp. were collected from five beaches (Khao Tao, Sea Pine Garden, Pran Buri Forest Park, Sam Roi Yod, and Sam Phraya) in Prachaupkhirikhan province, Thailand. Five morphological variable...Five hundred samples ofDonax spp. were collected from five beaches (Khao Tao, Sea Pine Garden, Pran Buri Forest Park, Sam Roi Yod, and Sam Phraya) in Prachaupkhirikhan province, Thailand. Five morphological variables of each individual were measured and analyzed to reveal morphological variation. The discriminant function analysis of morphology suggested a clear separation into two groups. The individuals from Khao Tao and Sea Pine Garden were grouped together while individuals from Pran Buri Forest Park, Sam Roi Yod and Sam Phraya were classified into the same group. The classification function of discriminant function analysis suggested that 70% of Khao Tao population, 70% of Sea Pine Garden population, 67% of Pran Buri Forest Park population, 64% of Sam Roi Yod population and 34% of Sam Phraya population of Donax spp. could be correctly reassigned by morphology. The misclassification individuals of Sam Phraya population were assigned to Pran Buri Forest Park and Sam Roi Yod population with the correct assignment 38% and 27%, respectively. The results obtained in this study based on morphological variation indicated that Khao Tao and Sea Pine Garden populations ofDonax spp. should be treated as separated units from the other three populations for conservation management. However, the result should be confirmed again as genetic level.展开更多
Truss network data were collected and investigated in order to clarify the morphological variation in populations of Coilia mystus from three Chinese estuaries. Nineteen morphometric measurements were made for each in...Truss network data were collected and investigated in order to clarify the morphological variation in populations of Coilia mystus from three Chinese estuaries. Nineteen morphometric measurements were made for each individual, and Burnaby's multivariate method was used to obtain size-adjusted shape data. The cluster analysis and discriminant analysis were used to discriminate morphological differences among populations. The results indicated that 1) the three populations were clustered into two distinct groups: the first group included Changiiang C. mystus and Zhujiang C. mystus, the last one included Minjiang C. mystus, and 2) discriminant analysis with selected 5 morphological parameters showed that the identification accuracy was between 98.7952% and 100%, and global identification accuracy was 99.2933%. Reproductive isolation and adaption to environmental condition are determinant factors for morphological variation between populations of C. mystus.展开更多
Morphological variation of snails from the genus Trochulus is so huge that their taxonomy is unclear. The greatest variability concerns forms hispidus and sericeus/plebeius, which are often considered as separate spec...Morphological variation of snails from the genus Trochulus is so huge that their taxonomy is unclear. The greatest variability concerns forms hispidus and sericeus/plebeius, which are often considered as separate species. To evidence the species barriers, we carried out crossbreeding ex- periments between these two sympatric morphs. Moreover, we compared the shell morphology of laboratory-bred offspring with their wild parents to test if the variation can be explained by the phenotypic plasticity model. We found that the two Trochulus morphs show no reproductive bar- riers. The fecundity rates, the mean clutch size, and F~ viability observed for all crosses were not significantly different. In hybrid crosses (in F2 generation), we also recorded reproduction compati- bility, similar fecundity, and hatching success as in their parents. Accordingly, phylogenetic ana- lyses revealed the significant grouping of sequences from these different morphs and supported no constrains in reproduction between them. Comparison of shell morphology between wild and laboratory samples showed that various characters appeared highly plastic. The average shell shape of the hispidus morph changed significantly from flat with wide umbilicus to elevated with narrower umbilicus such as in the sericeus/plebeius morph. All these findings indicate that the examined morphs do not represent separate biological species and the evolutionary process is not advanced enough to separate their genetic pool. Therefore, phenotypic plasticity has played a sig- nificant role in the evolution of Trochulus shell polymorphism. The two morphs can evolve inde- pendently in separate phylogenetic lineages under the influence of local environmental conditions.展开更多
基金Supported by Central Financial Forestry Science and Technology Promotion and Demonstration Project(2014HBTG07)~~
文摘With 50 large-flowered Chrysanthemum varieties from germplasm nursery of Wunaoshan Forest Farm in Macheng City as research objects, 64 morphological traits were investigated by field experiments adopting randomized block design. The morphological differences were observed by uniformity analysis, variability analysis, principal component analysis and cluster analysis. The result showed that the vari- able coefficients of 59 traits were greater than 15%; the contribution rate of first seven principal components reached 81.45%; and it was found by clustering analy- sis that the 50 germplasm resources could be divided into four clusters with obvious morphological differences, and plant type could be used as an index for classifica- tion.
文摘Five hundred samples ofDonax spp. were collected from five beaches (Khao Tao, Sea Pine Garden, Pran Buri Forest Park, Sam Roi Yod, and Sam Phraya) in Prachaupkhirikhan province, Thailand. Five morphological variables of each individual were measured and analyzed to reveal morphological variation. The discriminant function analysis of morphology suggested a clear separation into two groups. The individuals from Khao Tao and Sea Pine Garden were grouped together while individuals from Pran Buri Forest Park, Sam Roi Yod and Sam Phraya were classified into the same group. The classification function of discriminant function analysis suggested that 70% of Khao Tao population, 70% of Sea Pine Garden population, 67% of Pran Buri Forest Park population, 64% of Sam Roi Yod population and 34% of Sam Phraya population of Donax spp. could be correctly reassigned by morphology. The misclassification individuals of Sam Phraya population were assigned to Pran Buri Forest Park and Sam Roi Yod population with the correct assignment 38% and 27%, respectively. The results obtained in this study based on morphological variation indicated that Khao Tao and Sea Pine Garden populations ofDonax spp. should be treated as separated units from the other three populations for conservation management. However, the result should be confirmed again as genetic level.
基金The author would like to thank Mrs. Jindi Han, Mr. Xiaoguo Li, Mr. Guomin He, Mr. Zhenran Chen, and Dr. Jixing Zou for assistance in sampling and data collection. This work was supported by the National Natural Science Foundation of China (No. 30600456), Commission of Science and Technology of Shanghai, China (No. 08391910300), Project of Keji Xing Nong of Shanghai, China (No. 2008-7-2), open project of Key Laboratory for Sustainable Utilization of Marine Fisheries Resources Ministry of Agriculture (No. Shikai-2005-06), and special research fund for the national non-profit institutes (East China Sea Fisheries Research Institute, Project No. 2008Z02).
文摘Truss network data were collected and investigated in order to clarify the morphological variation in populations of Coilia mystus from three Chinese estuaries. Nineteen morphometric measurements were made for each individual, and Burnaby's multivariate method was used to obtain size-adjusted shape data. The cluster analysis and discriminant analysis were used to discriminate morphological differences among populations. The results indicated that 1) the three populations were clustered into two distinct groups: the first group included Changiiang C. mystus and Zhujiang C. mystus, the last one included Minjiang C. mystus, and 2) discriminant analysis with selected 5 morphological parameters showed that the identification accuracy was between 98.7952% and 100%, and global identification accuracy was 99.2933%. Reproductive isolation and adaption to environmental condition are determinant factors for morphological variation between populations of C. mystus.
文摘Morphological variation of snails from the genus Trochulus is so huge that their taxonomy is unclear. The greatest variability concerns forms hispidus and sericeus/plebeius, which are often considered as separate species. To evidence the species barriers, we carried out crossbreeding ex- periments between these two sympatric morphs. Moreover, we compared the shell morphology of laboratory-bred offspring with their wild parents to test if the variation can be explained by the phenotypic plasticity model. We found that the two Trochulus morphs show no reproductive bar- riers. The fecundity rates, the mean clutch size, and F~ viability observed for all crosses were not significantly different. In hybrid crosses (in F2 generation), we also recorded reproduction compati- bility, similar fecundity, and hatching success as in their parents. Accordingly, phylogenetic ana- lyses revealed the significant grouping of sequences from these different morphs and supported no constrains in reproduction between them. Comparison of shell morphology between wild and laboratory samples showed that various characters appeared highly plastic. The average shell shape of the hispidus morph changed significantly from flat with wide umbilicus to elevated with narrower umbilicus such as in the sericeus/plebeius morph. All these findings indicate that the examined morphs do not represent separate biological species and the evolutionary process is not advanced enough to separate their genetic pool. Therefore, phenotypic plasticity has played a sig- nificant role in the evolution of Trochulus shell polymorphism. The two morphs can evolve inde- pendently in separate phylogenetic lineages under the influence of local environmental conditions.