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缺乏形态学分化的非小细胞肺癌活检标本的进一步分类:一组实用的免疫组化试剂TTF-1,Napsin A,p63和CK5/6
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作者 解建军 张仁亚 《临床与实验病理学杂志》 CAS CSCD 北大核心 2011年第5期498-498,共1页
靶向治疗的应用越来越需要非小细胞肺癌(NSCLCs)的精确分型。该研究的目的评价一组免疫组化试剂在低分化非小细胞肺癌分型中的实用价值,并将活检组织的免疫组化结果与相应的术后标本进行对照分析。39例经切除后证实的NSCLC标本,活检... 靶向治疗的应用越来越需要非小细胞肺癌(NSCLCs)的精确分型。该研究的目的评价一组免疫组化试剂在低分化非小细胞肺癌分型中的实用价值,并将活检组织的免疫组化结果与相应的术后标本进行对照分析。39例经切除后证实的NSCLC标本,活检组织中均不能进一步分型。依据WHO分类标准对肿瘤切除标本进行分类。 展开更多
关键词 非小细胞肺癌 免疫组化 活检标本 分类标准 TTF-1 形态学分化 CK5/6 试剂
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缺乏形态学分化非小细胞性肺癌的免疫组化分型 被引量:1
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作者 翁映玲 《汕头大学医学院学报》 2014年第3期159-160,174,F0002,共4页
目的:探讨免疫组化在缺乏形态学分化的非小细胞性肺癌病理分型中的作用。方法:用一组6种抗体和免疫组化EnVision二步法对40例缺乏形态学分化的非小细胞性肺癌者进行检测。结果:本组鳞状细胞癌12例,腺癌23例,神经内分泌癌5例。本组CK7(62... 目的:探讨免疫组化在缺乏形态学分化的非小细胞性肺癌病理分型中的作用。方法:用一组6种抗体和免疫组化EnVision二步法对40例缺乏形态学分化的非小细胞性肺癌者进行检测。结果:本组鳞状细胞癌12例,腺癌23例,神经内分泌癌5例。本组CK7(62.5%)和甲状腺转录因子-1(55.0%)表达阳性率最高。结论:缺乏形态学分化的非小细胞性肺癌大多为腺癌,用6种抗体组成套餐的免疫组化检测能提高肺癌病理分型的诊断准确率。 展开更多
关键词 缺乏形态学分化 非小细胞性肺癌 免疫组织化学 病理分型
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104例胃癌功能分化与组织学分化的比较研究——利用酶组化、粘液组化方法 被引量:2
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作者 辛彦 赵凤凯 +2 位作者 吴东瑛 任常山 张荫昌 《中国医科大学学报》 CAS CSCD 1993年第4期259-262,共4页
本文采用酶组织化学和粘液组织化学方法,对104例胃癌的功能分化状态进行了系统观察。根据组化功能检测结果,将本组胃癌分为五个功能分化类型:1.吸收功能分化型;2.粘液分泌功能分化型;3.吸收—粘液分泌功能双向分化型;4.特殊功能分化型;5... 本文采用酶组织化学和粘液组织化学方法,对104例胃癌的功能分化状态进行了系统观察。根据组化功能检测结果,将本组胃癌分为五个功能分化类型:1.吸收功能分化型;2.粘液分泌功能分化型;3.吸收—粘液分泌功能双向分化型;4.特殊功能分化型;5.无功能分化型。研究结果表明:相同组织学类型的胃癌具有不尽相同的功能分化特征;不同功能分化的胃癌具有不同的浸润扩散能力。结果提示:单纯形态学描述在反映胃癌的生物学特征方面有一定的局限性。有必要增加功能分化的信息以弥补其不足。 展开更多
关键词 功能分化 形态学分化 胃肿瘤
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Bioaccumulation,subcellular distribution and chemical forms of cadmium in Aster subulatus Michx.
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作者 LIANG Xue-lian CHEN Wei +7 位作者 JIANG Wen-yan LIAO Jie YANG Yu-xia WANG Hai-jun LI Hui-ling LU Wei-fan WANG Tian-shun XIE Hong-zhao 《南方农业学报》 CAS CSCD 北大核心 2024年第8期2386-2395,共10页
【Objective】Through analyzing the bioaccumulation capacity,subcellular distribution and chemical forms of cadmium(Cd)in Aster subulatus Michx.,this study was to provide reference for revealing the Cd tolerance mechan... 【Objective】Through analyzing the bioaccumulation capacity,subcellular distribution and chemical forms of cadmium(Cd)in Aster subulatus Michx.,this study was to provide reference for revealing the Cd tolerance mechanism of A.subulatus Michx.【Method】After cultured for 24 d under the action of Hoagland nutrient solution and gradient Cd concentrations(0,30,60 and 90 mg/L),A.subulatus Michx.were harvested,and its leaf,stem and root were treated by differential centrifugation,chemical reagent extraction,and digested with graphite digester,respectively,then the Cd content in the root,stem and leaf were determined by atomic absorption spectroscopy.【Result】The experimental results indicated that the bioaccumulation capacity of Cd in A.subulatus Michx.was root>stem>leaf,and the maximum Cd concentration in the root,stem and leaf of A.subulatus Michx.were 130.74,78.69 and 56.62 mg/kg(fresh matter),respectively.Most of Cd stored in the cell wall and the soluble fractions of the root and leaf of A.subulatus Michx.,with only a smaller portion Cd in organelle fraction.Analysis result of subcellular Cd content showed that 52.27%-58.61%of Cd for root was mainly stored in the soluble fraction,but 42.10%-63.28%of Cd for leaf was mainly stored in the cell wall fraction.The concentration of pectates and protein integrated-Cd was higher in the root and leaf compared to other chemical forms Cd.Pectates and protein integrated-Cd was the main chemical forms Cd in the root and leaf of A.subulatus Michx.,and their percentages were 68.91%-74.80%and 57.38%-83.80%,respectively.Cd treatment could significantly increase the proportion of water-soluble organic acid Cd from 13.64%to 22.72%in root and undissolved phosphate Cd from 10.02%to 32.78%in leaf with increasing Cd concentration in the culture medium.【Conclusion】The root,stem and leaf of A.subulatus Michx.has strong bioaccumulation capacity to Cd,Cd is primarily stored in the soluble fractions of the root and cell wall fractions of the leaf,and less toxic pectates and protein integrated-Cd is the main chemical forms Cd in the root and leaf of A.subulatus Michx.,this might be the main mechanism of Cd tolerance in A.subulatus Michx. 展开更多
关键词 Aster subulatus Michx. CADMIUM BIOACCUMULATION chemical form subcellular distribution
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Soil Heavy Metal Pollution Around the Dabaoshan Mine,Guangdong Province,China 被引量:68
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作者 ZHOU Jian-Min DANG Zhi +1 位作者 CAI Mei-Fang LIU Cong-Qiang 《Pedosphere》 SCIE CAS CSCD 2007年第5期588-594,共7页
Soil contamination in the vicinity of the Dabaoshan Mine, Guangdong Province, China, was studied through determi- nation of total concentrations and chemical speciation of the toxic metals, Cu, Zn, Cd, and Pb, using i... Soil contamination in the vicinity of the Dabaoshan Mine, Guangdong Province, China, was studied through determi- nation of total concentrations and chemical speciation of the toxic metals, Cu, Zn, Cd, and Pb, using inductively coupled plasma mass spectrometry. The results showed that over the past decades, the environmental pollution was caused by a combination of Cu, Zn, Cd, and Pb, with tailings and acid mine drainage being the main pollution sources affecting soils. Significantly higher levels (P ≤ 0.05) of Cu, Zn, Cd, and Pb were found in the tailings as compared with paddy, garden, and control soils, with averages of 1486, 2516, 6.42, and 429 mg kg^-1, respectively. These metals were continuously dispersed downstream from the tallings and waste waters, and therefore their concentrations in the paddy soils were as high as 567, 1 140, 2.48, and 191 mg kg^-1, respectively, being significantly higher (P ≤ 0.05) as compared with those in the garden soils. The results of sequential extraction of the above metals from all the soil types showed that the residual fraction was the dominant form. However, the amounts of metals that were bound to Fe-Mn oxides and organic matter were relatively higher than those bound to carbonates or those that existed in exchangeable forms. As metals could be transformed from an inert state to an active state, the potential environmental risk due to these metals would increase with time. 展开更多
关键词 chemical speciation combined pollution index (CPI) contaminated soil heavy metals
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Equilibrium morphology of gas–liquid Janus droplets: A numerical analysis of buoyancy effect 被引量:1
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作者 Shaobin Zhang Helen Yan +2 位作者 Yuhao Geng Ke Wang Jianhong Xu 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2018年第10期2121-2126,共6页
In this article, a theoretical model for predicting the equilibrium morphology of gas–liquid Janus droplets was built. Based on this model, the effects of bubble radius and volume ratio on morphology change was syste... In this article, a theoretical model for predicting the equilibrium morphology of gas–liquid Janus droplets was built. Based on this model, the effects of bubble radius and volume ratio on morphology change was systematically studied. The increase of bubble radius causes the two parts(bubble and oil drop) in Janus droplets tend to merge while the impact of volume ratio is complicated. When volume ratio increases, these two parts firstly tend to merge, then gradually separate. The accuracy of this model was verified by experimental results. 展开更多
关键词 MICROFLUIDICS Gas–liquid–liquid emulsion Janus droplets
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Sequential Extraction of Aluminum and Iron from Acidic Soils by Chemical Selective Dissolution Methods 被引量:1
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作者 HEJIZHENG A.VIOLANTE 《Pedosphere》 SCIE CAS CSCD 1998年第1期37-44,共8页
Potassium chloride, Na-pyrophosphate, CuCl2, NH4-oxalate, dithionite-citrate-bicarbonate (DCB) and Na-citrate solutions were employed to extract aluminum (Al) and iron (Fe) sequentially and separately from 15 acidic s... Potassium chloride, Na-pyrophosphate, CuCl2, NH4-oxalate, dithionite-citrate-bicarbonate (DCB) and Na-citrate solutions were employed to extract aluminum (Al) and iron (Fe) sequentially and separately from 15 acidic soils located at the Mangshan Mountains, Hunan Province, China. Many evidences showed that separate pyrophosphate extracted mainly KCI-extractable Al, organo-Al complexes and some inorganic Al compounds, whereas separate CuCl2 extracted KCl-extractable Al and some organo-Al complexes. CuCl2 extracted much less amounts of Al than pyrophosphate did from the soils. Separate oxalate did not extract all KCl-Pyrophosphate- CuCl2 -oxalate sequentially extractable Al and Fe. Also, separate DCB did not extract all KCl- pyrophosphate- CuCl2 -oxalate- DCB sequentially extractable Al. The forms of Al extracted by oxalate and DCB from the soils were majorly noncrystalline. The interlayered materials of 1.4-nm intergrade minerals of the soils were attributed mainly to hydroxy Al polymers. 展开更多
关键词 acidic soil aluminum forms chemical selective dissolution
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Taxonomy of the lesser jerboa Jaculus jaculus (Dipodidae,Rodentia) based on allozymic and morphological variation
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作者 Abderraouf BEN FALEH Abdelwaheb BEN OTHMEN Khaled SAID 《Current Zoology》 SCIE CAS CSCD 北大核心 2010年第4期421-431,共11页
The lesser Egyptian jerboa Jaculus jaculus, is a member of the subfamily Dipodinae, known to have a number of subspecies. Previous studies of J. jaculus from North Africa found various morphological forms of unknown t... The lesser Egyptian jerboa Jaculus jaculus, is a member of the subfamily Dipodinae, known to have a number of subspecies. Previous studies of J. jaculus from North Africa found various morphological forms of unknown taxonomic status, which are frequently grouped into two taxa: J. jaculus and J. deserti. We assessed the taxonomic status of this taxon in Tunisia using allozymic (phylogenetic analysis of 23 genetic loci encoding 16 enzymatic proteins) and morphological (multivariate analysis of 13 craniodental characters) analyses of 136 J. jaculus specimens collected from seven localities within its distribution range. Allozymic analysis clearly indicated the existence of two distinct groups of J. jaculus, with a mean genetic distance (D) value of 0.99 and FΩ=0.52. Individuals within the two groups did not show any obvious geographical structuration, even within the Matmata locality which represents the meeting point for the two groups where they were found in sympatry. Additionally, morphometric analysis of 13 craniodental characters was clearly separated the two groups. These results were obtained over a very restricted area of the total distribution of J. jaculus and strongly suggest the occurrence of two separate and often sympatric species within the currently accepted J.jaculus in Tunisia: J.jaculus and J. deserti 展开更多
关键词 DIPODIDAE Jaculus jaculus TAXONOMY ALLOZYME Morphomotric analysis Tunisia
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Distribution of Chemical Forms for Co, Cr, Ni and V in Typical Soils of China 被引量:2
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作者 SHAOXIAO-HOU XINGGUANG-XI 《Pedosphere》 SCIE CAS CSCD 1993年第4期289-298,共10页
Co, Ni, Cr and V in 25 typical soils of China were fractionated into exchangeable, carbonate bound (calcareous soils), Mn oxide bound, organically bound, amorphous Fe oxide bound, crystalline Fe oxide bound and residu... Co, Ni, Cr and V in 25 typical soils of China were fractionated into exchangeable, carbonate bound (calcareous soils), Mn oxide bound, organically bound, amorphous Fe oxide bound, crystalline Fe oxide bound and residual forms using a seven-step sequential extraction procedure, so as to study the distribution of chemical forms of these metals as well as the effects of soil properties on the distribution. The results showed that most of soil Co, Ni, Cr and V were present in residual forms, and the distribution ratio averaged 48.2% for Co, 53.0% for Ni, 81.5% for Cr and 68.7% for V. The speciation of heavy metals was greatly influenced by soil physico-chemical properties and the chemistry of elements. The results also indicated that the recovery of metal elements by the sequential extraction procedure was satisfactory, with the relative difference between the sum of seven forms and the total content in soils averaging 9.5% for Co, 12.8% for Ni, 6.6% for Cr and 7.2% for V. 展开更多
关键词 distribution ratio metal element forms sequential extraction SOILS
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Research on the Structure and Properties of Mulberry Fiber
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作者 武海良 李冬梅 +2 位作者 吴长春 杨佩鹏 荆学谦 《Journal of Donghua University(English Edition)》 EI CAS 2008年第2期153-158,共6页
The chemical ingredients, morphological structure and properties of mulberry fiber have been researched. The results show that the mulberry fiber is cellulose fiber. The crystal structure of mulberry fiber is cellulos... The chemical ingredients, morphological structure and properties of mulberry fiber have been researched. The results show that the mulberry fiber is cellulose fiber. The crystal structure of mulberry fiber is cellulose I and the crystal cell belongs to the monoelinic system. The mulberry fiber crystal degree is 62.9% and orientation degree is 43.7%, the fineness of mulberry fiber is 2.3- 3.9 dtex, the length of the single fiber is 28 - 47 mm, the breaking strength of mulberry fiber is about 2.93 cN/dtex, and the initial modulus is about 76.82 cN/dtex. 展开更多
关键词 mulberry fiber chemical ingredients morphological structure
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The Morphological Analysis of Folktales of Tu Nationality in Hehuang Region in Northwest China
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作者 ZHAO Zhi-yi 《Journal of Literature and Art Studies》 2016年第11期1293-1303,共11页
The Tu nationality (also called "Tu people" or "Tus" in the paper), one of the multiple ethnic minorities in the Hehuang Region (河湟地区) between Gansu (甘肃) and Qinghai (青海) Provinces in the northwest... The Tu nationality (also called "Tu people" or "Tus" in the paper), one of the multiple ethnic minorities in the Hehuang Region (河湟地区) between Gansu (甘肃) and Qinghai (青海) Provinces in the northwest China, has no written language of their own, but has already created the abundant and varied colorful literary genres. Since the 1980s, folktales, as the main literary product popular in the region, have received literary criticisms and reviews from scholars and researchers in China, based on the socialist realism dominant in China in the 20th century that emphasized more on contents, motifs and even ideological significance. So far, no breakthroughs have been made in their studies even if some young scholars have tried from the formalist viewpoints. Therefore, this paper attempts to make a morphological analysis of the Tus' folktales by adopting Propp's theories, trying to find out the morphological narrative model beneath the language level of the folktales. Through the studies, we can see that collective awareness bonded by kinship is more emphasized in Tus' culture, and almost all tales end with good defeating evil. Therefore, the studies of these folktales will help us deep understand Tus' psychological conditions and positive cultural life. 展开更多
关键词 morphological analysis folktales of Tu nationality Hehuang Region Propp's theory creative rules collectiveness
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Effect of Long-Term in Vitro Sub-culturing on Quality Degeneration of Sweet Potato Varieties: Morpho- Anatomic Assessment and Simple Sequence Repeats Analysis
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作者 Mihiretu Cherinet Hundayehu Elsa Du Toit +3 位作者 Sunette Laurie Martin Steyn Ria Greyling Nokuthula Myeza 《Journal of Agricultural Science and Technology(A)》 2014年第10期811-821,共11页
The aim of this study was to assess the effect of long-term in vitro sub-culturing on the varietal degeneration of three sweet potato varieties, namely, Monate, Mokone and Ndou which were sub-cultured for 32, 23 and 1... The aim of this study was to assess the effect of long-term in vitro sub-culturing on the varietal degeneration of three sweet potato varieties, namely, Monate, Mokone and Ndou which were sub-cultured for 32, 23 and 12 generations, respectively. Each generation was cultured in a media which is made from 4.43 g/L Murashige and Skoog (MS), 30 g/L sucrose and 2 g/L gelrite, respectively, and grown under 16 h light and 8 h dark photoperiod for 30 d. For each generation, 45 plantlets were acclimatized for two months in a glasshouse. Data on in vitro growth performance and 11 morphological characteristics during acclimatization were recorded. Early root and shoot formation was observed after the 27th and 21st sub-cultured generations of Monate and Mokone, respectively. During acclimatization, plantlets from the same variety showed differences in morphological traits such as leaf colour, abaxial leaf pigmentation, vine pigmentation, petiole pigmentation, leaf wrinkling and flowering. However, the rate of these morphological differences is random and irrespective to increase in sub-culturing. Therefore, to understand the genetic base of these morphological variability, two plantlets from each variety were subjected to genetic analysis by using five simple sequence repeat (SSR) primers (IB-242, IB-318, IB-255F, 1B-248 and IB-255). Although SSR loci IB-255F and IB-318 could distinguish between the three varieties, there were no allelic polymorphisms detected in plantlets from the same varieties. Therefore, long-term sub-culturing do not leads to quality degeneration in the three sweet potato varieties. 展开更多
关键词 MICRO-PROPAGATION ORGANOGENESIS SUB-CULTURE true-to-type morphology polymorphism.
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Melanopsin-expressing retinal ganglion cell loss and behavioral analysis in the Thy1-CFP-DBA/2J mouse model of glaucoma 被引量:4
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作者 ZHANG Qi VUONG Helen +4 位作者 HUANG Xin WANG YanLing BRECHA Nicholas C. PU MingLiang GAO Jie 《Science China(Life Sciences)》 SCIE CAS 2013年第8期720-730,共11页
In this study, the role of melanopsin-expressing retinal ganglion cells (mRGCs) in the glaucoma-induced depressive behavioral response pattern was investigated. The CFP-D2 transgenic glaucoma animal model from five ... In this study, the role of melanopsin-expressing retinal ganglion cells (mRGCs) in the glaucoma-induced depressive behavioral response pattern was investigated. The CFP-D2 transgenic glaucoma animal model from five age groups was used in this study. Immunohistochemical labeling, quantitative analysis of mRGC morphology, open field test (OFT), and statistical analysis were used. In comparison with C57 BL/6 mice, the age-matched CFP-D2 mice had significantly elevated intraocular pressure (lOP). We observed parallel morphological changes in the retina, including a reduction in the density of cyan fluorescent protein- (CFP) expressing cells (cells mm^-2 at 2 months of age, 1309±26; 14 months, 878±30, P〈0.001), mRGCs (2 months, 48_+3; 14 months, 19±4, P〈0.001), Brn3b-expressing RGCs (2 months, 1283±80; 14 months, 950±31, P〈0.001), Brn-3b expressing mRGCs (5 months, 50.17%±5.5%; 14 months, 12.61%±3.8%, P〈0.001), and reduction in the dendritic field size of mRGCs (mm^2 at 2 months, 0.077±0.015; 14 months, 0.065±0.015, P〈0.05). CFP-D2 mice had hyperactive locomotor activity patterns based on OFT findings of the total distance traveled, number of entries into the center, and time spent in the center of the testing apparatus. The glaucoma induced hyperactive response pattern could be associated with dysfunctional mRGCs, most likely Brn-3b-positive mRGCs in CFP-D2 mice. 展开更多
关键词 pigmentary glaucoma Thyl-CFP-DBA/2J mouse retinal ganglion cells melanopsin-expressing retinal ganglion cells depression
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Morphologic studies of high fall injuries 被引量:1
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作者 XIA Peng CHANG Hong-fa +4 位作者 YU Yong-min DAI Guo-xin LI Hong-wei JIANG Qiang-guo YIN Zhi-yong 《Chinese Journal of Traumatology》 CAS 2012年第6期334-337,共4页
[Abstract] Objective: By studying the mode and morphologic character of high fall injuries on the scene, and exploring the injury situation of different heights, different fall ways and postures, to provide a referen... [Abstract] Objective: By studying the mode and morphologic character of high fall injuries on the scene, and exploring the injury situation of different heights, different fall ways and postures, to provide a reference for the foren- sic identification of high fall injury. Methods: All the high fall cases were statistically analysed according to their gender, age, ground-touching posture, fall height, site and type of the injury. Results: Among 134 high fall cases, 98 were male and 36 were female with the age ranging from 2-71 years (37.6 +16.9 on average), in which, 10-60 years old group con- sisted of 110 cases (82%). Most cases fell from windows orroofs (73%) and the touching objects were cement ground or shaft bottom of elevators. Among these cases, head injury was generally serious, followed by chest and abdominal injuries. The morphologic changes depend upon the height, nature, as well as the posture at the point while the body touches the ground. Conclusion: Morphologic study of high fall injury assists medicolegal physicians to make correct identifica- tions of the cause and nature of high fall injuries. 展开更多
关键词 Wounds and injuries Forensic medicine Anatomy and histology
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Study of Substrate and Physico-Chemical Base Classification of the Rivers of Nepal
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作者 JHA Bibhuti Ranjan WAIDBACHER Herwig +1 位作者 SHARMA Subodh STRAIF Michael 《Geo-Spatial Information Science》 2010年第1期70-76,共7页
The rivers in Nepal are classified in terms of geographical regions but a more scientific classification such as on the ba-sis of morphology is clearly lacking. This study was done in 9 rivers namely Jhikhukhola of th... The rivers in Nepal are classified in terms of geographical regions but a more scientific classification such as on the ba-sis of morphology is clearly lacking. This study was done in 9 rivers namely Jhikhukhola of the Koshi system, Aandhikhola, Arungkhola, East Rapti, Karrakhola, Seti and main channel Narayani of the Gandaki system, and two independent systems within Nepal, Bagmati and Tinau. Among the morphologies, river bed or the substratum was taken as the main variable for the analysis which was categorized into 7 types as rocks, boulders, cobbles, pebbles, gravels, sand and silt. There were 23 sampling sites each with 2 stretches of around 100m in those rivers. The data were taken as a percentage, and to avoid biases it was observed visually by the same person for a complete year in every season. With 23 sites each with 2 stretches and 4 replicates corresponding to 4 seasons, there are altogether 184 observations, each termed as a case, that constitute this work. Canonical Discrimination Analysis (CDA) which is most suitable when the data pool is huge was applied to see if the rivers studied distinguish themselves in terms of its morphology. The result was remarkably successful and was close to the established regional classification of the rivers. This kind of river classification has great application in the utilization, conservation and restoration of the most important natural re-source of the country. 展开更多
关键词 Nepal rivers morphology physico-chemical parameter river classification
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