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基质辅助激光解吸电离飞行时间质谱技术在临床感染性曲霉菌快速鉴定中的应用 被引量:2
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作者 沈松坤 陈涌泉 《国际医药卫生导报》 2018年第6期918-920,共3页
目的探讨基质辅助激光解吸电离飞行时间质谱(MALDI—TOFMS,VITEKMSV3.0)技术在临床侵袭性曲霉菌鉴定中的应用价值。方法分离来自于临床侵袭性曲霉菌感染患者标本中的曲霉菌,在不同的真菌培养条件下进行纯培养,应用MALDI—TOFMS技... 目的探讨基质辅助激光解吸电离飞行时间质谱(MALDI—TOFMS,VITEKMSV3.0)技术在临床侵袭性曲霉菌鉴定中的应用价值。方法分离来自于临床侵袭性曲霉菌感染患者标本中的曲霉菌,在不同的真菌培养条件下进行纯培养,应用MALDI—TOFMS技术对其进行鉴定,并与传统形态学鉴定技术鉴定结果进行比较分析。结果88株菌株分别在沙保弱液体培养基、沙保弱琼脂培养基和察式琼脂培养基上培养,MALDI—TOFMS技术鉴定正确率分别为95.74%、95.45%和95.17%,形态学技术鉴定正确率分别为82.10%、86.08%和81.82%,不同培养基上培养鉴定正确率差异均具有统计学意义(χ2=136.936,P=O.000;χ2=75.918,P=O.000;χ2=122.612,P=O.000);88株菌株分别培养48h、72h、96h和120h,MALDI—TOFMS技术鉴定正确率分别为90.15%、97.35%、97.35%和96.97%,形态学技术鉴定正确率分别为77.27%、82.58%、85.23%和88.26%,不同培养时间鉴定正确率差异均具有统计学意义(χ2=65.824,P=O.000;χ2=132.021,P=0.000;χ2=93.598,P=O.000;χ2=61.382,P=O.000)。结论MALDI—TOFMS技术对临床侵袭性曲霉菌鉴定的准确率高于形态学鉴定技术,可在临床侵袭性曲霉菌鉴定中推广应用。 展开更多
关键词 基质辅助激光解吸电离飞行时间质谱 MALDI-TOF MS 形态学鉴定技术 曲霉菌
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Paternity Testing of Selected Abaca (Musa textilis L. Nee) Hybrids Using Morphometric Markers
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作者 F. C. L. Zapico C. H. M. Aguilar J. M. Aujero B. Y. Disca 《Journal of Agricultural Science and Technology》 2010年第5期101-105,共5页
Investigation into the paternity of four abaca (Musa textilis L, Nee) hybrids was done to ascertain the mode of transmission of selected morpho-agronomic traits and to detect possible heterosis. In situ morphologica... Investigation into the paternity of four abaca (Musa textilis L, Nee) hybrids was done to ascertain the mode of transmission of selected morpho-agronomic traits and to detect possible heterosis. In situ morphological characterization was undertaken using twenty five qualitative and six quantitative characters. Results revealed that a great majority of the qualitative traits were shared by both parents and their hybrids. For the rest, the qualitative traits were inherited from one or the other parent though some variant phenotypes (i.e. chimerism) were also noted in the hybrids. Cases ofheterosis were also observed and this could be exploited to increase fiber yield in the hybrids. Though inconclusive due to factors such as the heterogenous nature of abaca plants in the field and the susceptibility of morphological traits to environmental fluctuations, this study has provided baseline information on abaca hybridity that can be verified using more robust technologies as molecular markers. 展开更多
关键词 Abaca (Musa textilis L. Nee) paternity testing morphological characterization heterosis.
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