Integrative analysis of bone-formation associated genes and immune cell infiltration in osteoporosis, and the prediction of active ingredients in targeted traditional Chinese medicine

Objective To explore the differential expression and mechanisms of bone formation-related genes in osteoporosis(OP)leveraging bioinformatics and machine learning methodologies;and to predict the active ingredients of targeted traditional Chinese medicine(TCM)herbs.Methods The Gene Expression Omnibus(GEO)and GeneCards databases were employed to conduct a comprehensive screening of genes and disease-associated loci pertinent to the pathogenesis of OP.The R package was utilized as the analytical tool for the identification of differentially expressed genes.Least absolute shrinkage and selection operator(LASSO)logis-tic regression analysis and support vector machine-recursive feature elimination(SVM-RFE)algorithm were employed in defining the genetic signature specific to OP.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses for the selected pivotal genes were conducted.The cell-type identification by estimating rela-tive subsets of RNA transcripts(CIBERSORT)algorithm was leveraged to examine the infiltra-tion patterns of immune cells;with Spearman’s rank correlation analysis utilized to assess the relationship between the expression levels of the genes and the presence of immune cells.Coremine Medical Database was used to screen out potential TCM herbs for the treatment of OP.Comparative Toxicogenomics Database(CTD)was employed for forecasting the TCM ac-tive ingredients targeting the key genes.AutoDock Vina 1.2.2 and GROMACS 2020 softwares were employed to conclude analysis results;facilitating the exploration of binding affinity and conformational dynamics between the TCM active ingredients and their biological targets.Results Ten genes were identified by intersecting the results from the GEO and GeneCards databases.Through the application of LASSO regression and SVM-RFE algorithm;four piv-otal genes were selected:coat protein(CP);kallikrein 3(KLK3);polymeraseγ(POLG);and transient receptor potential vanilloid 4(TRPV4).GO and KEGG pathway enrichment analy-ses revealed that these trait genes were predominantly engaged in the regulation of defense response activation;maintenance of cellular metal ion balance;and the production of chemokine ligand 5.These genes were notably associated with signaling pathways such as ferroptosis;porphyrin metabolism;and base excision repair.Immune infiltration analysis showed that key genes were highly correlated with immune cells.Macrophage M0;M1;M2;and resting dendritic cell were significantly different between groups;and there were signifi-cant differences between different groups(P<0.05).The interaction counts of resveratrol;curcumin;and quercetin with KLK3 were 7;3;and 2;respectively.It shows that the interac-tions of resveratrol;curcumin;and quercetin with KLK3 were substantial.Molecular docking and molecular dynamics simulations further confirmed the robust binding affinity of these bioactive compounds to the target genes.Conclusion Pivotal genes including CP;KLK3;POLG;and TRPV4;exhibited commendable significant prognostic value;and played a crucial role in the diagnostic assessment of OP.Resveratrol;curcumin;and quercetin;natural compounds found in TCM;showed promise in their potential to effectively modulate the bone-forming gene KLK3.This study provides a sci-entific basis for the interpretation of the pathogenesis of OP and the development of clinical drugs.

青藏高原东南缘兰坪-思茅盆地反S型构造属性的古地磁约束

兰坪—思茅(盆地)内部发育的一系列褶皱和逆冲断层组成了显著的弧形构造,其中部呈现反S形构造形态。该地区地壳构造变形方式及其演化过程与青藏高原东南缘地壳物质新生代侧向挤出逃逸密切相关,对其开展地壳构造变形方式的定量分析,具有重要的意义。本文通过兰坪—思茅盆地中部反S形构造区域四条白垩纪红层剖面的岩石磁学和古地磁学研究,获得南涧牛街地区早白垩世LN-a和LN-b剖面的特征剩磁方向分别为D_s=244.7°,I_s=-47.3°,Ks=31.6,α_(95)=16.6°(K_1j^1),和D_s=245.4°,I_s=-46.4°,K_s=52.2,α_(95)=9.4°(K_1n^2),两剖面在99%置信度下通过了Mc Fadden(1990)褶皱检验;小湾镇地区早白垩世NX-a和NX-b剖面(两套地层分属轴向不同的两褶皱)特征剩磁方向分别为D_s=244.9°,I_s=-40.2°,K_s=1067.5,α_(95)=2.3°(K_1j^1)和D_s=182.6°,I_s=-42.1°,K_s=335.2,α_(95)=3.7°(K_1j^2)。结合前人在兰坪—思茅地体中部获得的可靠古地磁数据及青藏高原东南缘区域新生代断裂活动演化过程的分析,指出兰坪—思茅盆地中部弧形反S形构造的形成和演化,以及兰坪—思茅盆地构造区内部差异性旋转变形作用主要受控于新生代时期青藏高原东南缘地壳块体侧向旋转挤出过程中的相互挤压作用和临沧花岗岩基对兰坪—思茅地体侧向顺时针旋转挤出运动的阻挡等一系列构造因素。

Driving Behavior Shaping Model in Road Traffic System

In order to give a new way for modeling driving behavior, identifying road traffic accident causation and solving a variety of road traffic safety problems such as driving errors prevention and driving behavior analysis, a new driving behavior shaping model is proposed, which could be used to assess the degree of effect of driving error upon road traffic safety. Driver behavior shaping model based on driving reliability and safety analysis could be used to identify the road traffic accident causation, to supply data for driver's behavior training, to evaluate driving procedures, to human factor design of road traffic system.

Tribological behaviors between commercial pure titanium sheet and tools in warm forming

The tribological conditions between tools and sheet are the major factors affecting the product quality,forming limits and service life of tools in thin-walled titanium components warm forming.Using the orthogonal design based twist compression test in the temperature range of 25-300 °C,the significant factors affecting the coefficient of friction（Co F） and the influencing rules in CP-3 titanium sheet warm forming are clarified and discussed by changing tribological conditions such as tool material,lubrication,temperature and normal pressure.The results show that the significant factors affecting the Co F are lubrication,surface roughness,tool material,sliding velocity,normal pressure and temperature;compared with unlubricated condition,the graphite and Mo S2 greatly improve the friction condition and the maximum reduction of the Co F is 0.318;the Co Fs of Cr12 Mo V/CP-3 and QAl10-3-1.5/CP-3 tribo-pairs show a similar tendency：the Co Fs increase with increasing surface roughness and sliding velocity,and increase firstly then decrease with increasing normal pressure and temperature.

Advance and trend of friction study in plastic forming

Friction is a critical issue in plastic forming which influences forming force, metal flow, forming quality and service life of die. Since friction is a highly nonlinear physical phenomenon which is interactively affected by so many factors, great efforts have been made to study the friction mechanism and controlling. The research progress of friction issues in plastic forming was summarized and discussed from four aspects： testing, characterizing, modeling and optimization/controlling. Considering urgent demands for green, efficient and precise forming of high-performance, lightweight and complex components in high-tech industries such as aerospace and automotive, the trends and challenges of friction study in plastic forming were proposed.

基于高斯脉冲UWB信号特性的研究

通过对高斯形脉冲UWB信号的数学框架研究,得到改变高斯脉冲行成因子和对高斯脉冲进行微分运算可以提高脉冲峰值频率和带宽的结论,由此能够获得理想的UWB脉冲。仿真结果表明,基于高斯脉冲组合可以获得逼近FCC标准的UWB信号,为设计UWB脉冲信号提供了理论依据。

Effects of Rosiglitazone and Serum on the Expressions of PPARα and PPARγ Genes in the Induced Differentiation Process of Pig Preadipocyte

[Objective] The research aimed to discuss the effects of rosiglitazone and serum on the expressions of PPARα and PPARγ genes in the induced differentiation process of pig preadipocyte.[Method] The pig preadipocyte was separated by using the collagenase digestion method.Three kinds of different differentiation culture solutions were used to induce the differentiation of pig preadipocyte.The oil red O staining extraction method was used to contrast the influences of different differentiation culture solutions on the variation of cellular fat content in the differentiation process.Moreover,the variation trends of PPARα and PPARγ expressions in the cellular differentiation process in the different differentiation culture solutions were detected by the real-time quantification PCR.[Result] The cellular fat accumulation was the fastest in MII which contained rosiglitazone and was the slowest in MI which didn＇t contain rosiglitazone.Rosiglitazone could significantly increase the expression of PPARγ gene（P0.01）,but had the certain inhibition effect on the expression of PPARα gene,which wasn＇t significant.The serum had the extremely significant up-regulation effect on the expression of PPARγ gene（P0.01）,but had the extremely significant down-regulation effect on the expression of PPARα gene（P0.01）.[Conclusion] Rosiglitazone could greatly promote the expression of PPARγ gene,which increased the cellular fat deposition.Maybe the activator of PPARγ gene existed in the serum,and the inhibitor of PPARα gene existed simultaneously.

浅谈沥青路面裂缝处治技术

本文介绍了沥青路面裂缝形成的原因,路面裂缝的主要形式,以及裂缝的预防。以处治S220线路面裂缝为例,重点介绍了裂缝处治的施工工艺,并提出了沥青路面裂缝处治的新方向。

Effect of friction coefficient in deep drawing of AA6111 sheet at elevated temperatures

The effect of friction coefficient on the deep drawing of aluminum alloy AA6111 at elevated temperatures was analyzed based on the three conditions using the finite element analysis and the experimental approach.Results indicate that the friction coefficient and lubrication position significantly influence the minimum thickness,the thickness deviation and the failure mode of the formed parts.During the hot forming process,the failure modes are draw mode,stretch mode and equi-biaxial stretch mode induced by different lubrication conditions.In terms of formability,the optimal value of friction coefficient determined in this work is 0.15.At the same time,the good agreement is performed between the experimental and simulated results.Fracture often occurs at the center of cup bottom or near the cup corner in a ductile mode or ductile-brittle mixed mode,respectively.

DETERMINATION OF PRESSURIZING CURVE AND COMPUTER CONTROL OF THICKNESS THINNING FOR SUPERPLASTIC BULGING

This paper proposes the assumption that the flow with viscous friction is the stretch of part of the sheet that lies along the walls of a die during the process of superplastic bulging according to superplastic flow equation and geometrical model of bulging of a sheet into a long trapezoid groove or truncated cone, by introducing the friction-factor P which describes the friction effect on the process. Also, the paper proposes the method of controlling thickness nonuniformity and develops the equipment which for uniform thickness of bulging, is automatically controlled with a computerl it also analyzes the important innuence of lubrication on thickness distribution of bulging materials. By the assumption, the relationship between bulging pressure and time is obtained in bulging of a sheet into the groove and cone, and p-t curve of multi-mould-cavity complicated bulging is discussed based on the analysis of single-mould-cavity bulging characteristics.

Antisense angiopoietin-1 inhibits tumorigenesis and angiogenesis of gastric cancer

AIM： To investigate the effect of angiopoietin-1 （Ang-1） on biological behaviors in vitro and tumorigenesis and angiogenesis in vitro of human gastric cancer cells. METHODS： Human full-length Ang-1 gene was cloned from human placental tissues by RT-PCR method. Recombinant human Ang-1 antisense eukaryotic expression vector was constructed by directional cloning, and transfected by lipofectin method into human gastric cancer line SGC7901 with high Ang-1 expression level. Inhibition efficiency was confirmed by semi- quantitive PCR and Western blot method. Cell growth curve and cell cycle were observed with MTT assays and flow cytometry, respectively. Nude mice tumorigenicity test was employed to compare in vitro tumorigenesis of cells with Ang-1 suppression. Microvessel density （MVD） of implanted tumor tissues was analyzed by immunohistochemistry for factor VIII staining. RESULTS： Full-length Ang-1 gene was successfully cloned and stable transfectants were established, namely 7Ang1- for antisense, and 7901P for empty vector transfected. 7Ang1- cells showed down-regulated Ang-1 expression, while its in vitro proliferation and cell cycle distribution were not significantly changed. In contrast, xenograft of 7Ang1- cells in nude mice had lower volume and weight than those of 7901P after 30 days＇ implantation （P〈 0.01, 293.00：1±95.54 mg vs. 624.00±77.78 mg） accompanied with less vessel formation with MVD 6.00±1.73 compared to 7901P group 8.44±1.33 （P〈 0.01）. CONCLUSION： Ang-1 may play an important role in tumorigenesis and angiogenesis of gastric cancer, and targeting its expression may be beneficial for the therapy of gastric cancer.

Etiology and consequences of thrombosis in abdominal vessels

The thrombophilia which can be either congenital or acquired in adult life has major implications in the abdominal vessels. The resulting portal vein thrombosis, Budd-Chiari syndrome and mesenteric vein thrombosis have a variety of consequences ranging from acute abdomen to chronic hepatomegaly and even totally asymp- tomatic patient in whom the only finding is pancytopenia. The complications like esophageal varices, portal gastropathy, ascites, severe hypersplenism, liver failure requiring liver transplantation are well known. Interesting features of collateral venous circulation showing itself as pseudocholangiocarcinoma sign and its possible clinical reflection as cholestasis are also known from a long time. The management strategies for these complications of intraabdominal vessel thrombosis are not different from their counterpart which is cirrhotic portal hypertension, but the prognosis is unquestionably better in former cases. In this review we presented and discussed the abdominal venous thrombosis, etiology and the resulting clinical pictures. There are controversial issues both in nomenclature, and management including anticoagulation problems and follow up strategies. In light of the current knowledge, we discussed some controversial issues in literature and presented our experience and our proposals about this group of patients.

GIS-Based and Data-Driven Bivariate Landslide-Susceptibility Mapping in the Three Gorges Area, China

A detailed landslide-susceptibility map was produced using a data-driven objective bivariate analysis method with datasets developed for a geographic information system （GIS）. Known as one of the most landslide-prone areas in China, the Zhongxian-Shizhu Segment in the Three Gorges Reservoir region of China was selected as a suitable case because of the frequency and distribution of landslides. The site covered an area of 260.93 km^2 with a landslide area of 5.32 km^2. Four data domains were used in this study, including remote sensing products, thematic maps, geological maps, and topographical maps, all with 25 m × 25 m pixels. Statistical relationships for landslide susceptibility were developed using landslide and landslide causative factor databases. All continuous variables were converted to categorical variables according to the percentile divisions of seed cells, and the corresponding class weight values were calculated and summed to create the susceptibility map. According to the map, 3.6% of the study area was identified as high-susceptibility. Extremely low-, very low-, low-, and medium-susceptibility zones covered 19.66%, 31.69%, 27.95%, and 17.1% of the area, respectively. The high- and medium-hazardons zones are along both sides of the Yangtze River, being in agreement with the actual distribution of landslides.

The application of combined gravity and seismic data formation separation for revealing deep structure

In some oilfields with 3D seismic data, the deeper structure cannot be observed due to poor quality deep seismic data. Layer stripping using both seismic and gravity data is a solution for this problem but it cannot get satisfactory results because the horizontal variations in formation density are ignored. We present a variable-density formation separation technique to address this problem. Based on 3D seismic depth data and laterallyvariable density derived from 3D seismic velocity data, the upper formation gravity effect is calculated by forward modeling and removed from the Bouguer gravity. The formation-separated gravity anomaly with variable density is obtained, which mainly reflects the deeper geological structure. In block XX of North Africa, the shallow formations seismic data is excellent but the data at the top of basement is poor. The formation-separated gravity anomaly processed under the control of 3D seismic data fits well with the known seismic interpretation and wells. It makes the geological interpretation more reliable.

Mesenteric and portal vein thrombosis associated with hyperhomocysteinemia and heterozygosity for factor V Leiden mutation

A 79-year-old man was hospitalized because of worsening upper abdominal pain which started two days before admission and was continuously present. His personal and family historywas uneventful, he did not smoke and denied toxic habits or using any medications, including overthe-counter medications, herbal remedies or any vitamin supplements.

The Partial Purification of Angiogenesis Factor of Human Osteosarcoma

Objective To partially purify the angiogenesis factor of human osteosarcoma(HuOs) and study its biological features. Methods The active peptide with a molecular weight of 8000-10000 Da in the conditioned medium obtained from the cultivation of Hu-Os cells(osteoblastic osteosarcoma) was partially purified by ultrafiltration, chromatography and dialysis.The angiogenic effects of the fractions were assessed by proliferation assay of human umbilical vein and pig thoracic aorta endothelial cells. Results The chromatography fractions 4-6 could significantly promote the proliferation of the endothelial cells.Conclusion The HuOs cells could synthesize and secrete angiogenesis factor with a molecular weight of 8000-10000 Da.

Effect of basic fibroblast growth factor(bFGF) on the treatment of exposure of the orbital implants

Objective: To evaluate the efficacy and the indication of basic fibroblast growth factor (bFGF) in the treatment of exposure of orbital implants. Design: Retrospective and observational case series. Methods: We reviewed 41 patients (41 eyes) suffering exposure of orbital implants from Jan. 2000 to June 2006. The study group patients with mild exposure received com-bined treatment with bFGF and antibiotic drops, and while the control group patients with mild exposure were treated with anti-biotic drops only. The study group patients with moderate and severe exposure received combined treatment with bFGF and antibiotic drops, and after 2 months they were subjected to amniotic membrane transplantation, while the control group patients with moderate and severe exposure underwent amniotic membrane transplantation after using antibiotic drops. Observation of the growth of conjunctival epithelium and comparison of the healing rate of the two groups. Results: The healing rates of the mild, moderate and severe exposure study group were 100% and 92.3%. The healing rates of the mild, moderate and severe exposure control group were 55.6% and 66.7% respectively. The difference of the healing rates of the mild exposure study group and the control group was significant (P=0.033). And the difference of the healing rates of the moderate and severe exposure study group and the control group was not significant (P=0.167). Conclusion: bFGF may promote obviously the healing of orbital implant exposure, particularly it can be the first choice for the treatment of mild degree exposure. For the moderate and severe cases, it can be administered before surgical repair to enhance neovascularization and will tend to increase the success rate of surgical repair.

Regulated expression of TATA-binding protein-related factor 3(TRF3)during early embryogenesis

RNA polymerase （Pol） Ⅱ transcription persists in TATA-box-binding protein （TBP）^-/- mutant mouse embryos, indicating TBP-independent mechanisms for Pol Ⅱ transcription in early development. TBP-related factor 3 （TRF3） has been proposed to substitute for TBP in TBP^-/- mouse embryos. We examined the expression of TRF3 in maturing oocytes and early embryos and found that TRF3 was co-expressed with TBP in the meiotic oocytes and early embryos from the late one-cell stage onward. The amounts of TBP and TRF3 changed dynamically and correlated well with transcriptional activity. Chromatin immunoprecipitation （CHIP） assay revealed that different gene promoters in mouse embryonic stem （ES） cells recruited TRF3 and TBP selectively. Comparative analyses of TRF3 and TBP during cell cycle showed that both factors proceeded through cell cycle in a similar pace, except that TRF3 was slightly delayed than TBP in entering the nucleus when cells were exiting the M-phase. Data from expression and biochemical analyses therefore support the hypothesis that TRF3 plays a role in early mouse development. In addition, results from co-localization study suggest that TRF3 may be also involved in Pol Ⅰ transcription.

Causes Leading to Formation of Shot Cokes and Measures for Its Prevention in Super Viscous Oil Delayed Coking Unit

Shot cokes are frequently formed in the delayed coking unit treating the super viscous oil at PetroChina＇s Liaohe Petrochemical Company. Considerable work has been carded out to avoid the formation of shot cokes. The test results obtained have shown that the property of super viscous oil has played a key role in the formation of shot cokes. After adjusting and optimizing the process indices the operating regime of the delayed coking unit at a throughput of 118t/h of the super viscous oil is specified as follows： a reaction temperature of 498-502℃, a reaction pressure of 0.17-0.25 MPa, a recycle ratio of 0.5-0.6 and a fractionation tower bottom temperature of 355-365 ℃. In the meantime, the delayed coking process has adopted measures to enhance pre-fractionation of the feedstock to rationally remove light fractions and maintain a steady gas velocity in order to avoid the formation of shot cokes.

Numerical Simulation of NO_x Formation in Coal Combustion with Inlet Natural Gas Burning

A full two-fluid model of reacting gas-particle flows and coal combustion is used to simulate coal combustion with and without inlet natural gas added in the inlet. The simulation results for the case without natural gas burning is in fair agreement with the experimental results reported in references. The simulation results of different natural gas adding positions indicate that the natural gas burning can form lean oxygen combustion enviroment at the combustor inlet region and the NOx concentration is reduced. The same result can be obtained from chemical equilibrium analysis.