银杏受精的形质变化及其种胚生长发育的候周期观察

【目的】探究银杏种实受精时的内、外部形态变化;观察种胚发育的结构及数量变化规律。【方法】在7月中旬银杏种实形态建成后,以5 d为周期连续采样,观察其受精前、中、后期的外种皮颜色变化;制作系列石蜡切片,使用光学显微镜和电子显微镜观察种胚结构的内部变化。统计分析不同时期“圆球胚、小子叶胚、大子叶胚、近成熟胚、完全成熟胚”5种类型种胚比例。【结果】五天一候的频度观察发现,银杏外种皮由绿转黄是受精的外部形质标志;外种皮由圆润变得缩皱,是胚胎开始发育的外部形态指标。8月中旬完成受精,形成合子;9月初,种胚肉眼可见,此时绝大多数为圆球胚和小子叶胚;9月下旬,小子叶胚占比最多(85%);到10月中旬,大子叶胚占比数量上升(63%);至11月上旬,近成熟胚占比为71%;到翌年1月下旬,57%已达完全成熟胚阶段。【结论】采用气象候周期采样频度,辅以光镜、电镜显微技术,首次获得了银杏在受精以及种胚始育时段种实的外部形质变化高精图;以候周期统计了种胚发育过程中相应的各种胚类型的数量比例。在研究过程中,新发现银杏种胚的子叶表面已经具气孔,中部具螺纹管胞,胚轴具维管束;首次大数据统计获得约1.5%的银杏种实有双胚发育现象,这些研究结果丰富了对银杏生殖发育的认识。

Si phase morphology and mechanical properties of ZL107 Al alloy improved by La modification and heat treatment

The phase morphology evolution during the solid solution treatment and then artificial aging of the La-modified ZL107 Al alloy was studied. The results show that when the solid solution was held at 560 ℃ for 6 h, only partial Si phase dissolved into the matrix; however, the precipitation also occurred during the artificial aging process. The precipitation process in Al-Si alloys with or without La-modification was compared. After modification and heat treatment, the mechanical properties of the alloy were greatly enhanced, due to the modification and uniform distribution of Si phase.

Design concerns of room and pillar retreat panels

Why do some room and pillar retreat panels encounter abnormal conditions？ What factors deserve the most consideration during the planning and execution phases of mining and what can be done to mitigate those abnormal conditions when they are encountered7 To help answer these questions, and to determine some of the relevant factors influencing the conditions of room and pillar （R ＆ P） retreat min- ing entries, four consecutive R ＆ P retreat panels were evaluated. This evaluation was intended to rein- force the influence of topographic changes, depth of cover, multiple-seam interactions, geological conditions, and mining geometry. This paper details observations were made in four consecutive R ＆ P retreat panels and the data were collected from an instrumentation site during retreat mining. The pri- mary focus was on the differences observed among the four panels and within the panels themselves. The instrumentation study was initially planned to evaluate the interactions between primary and secondary support, but produced rather interesting results relating to the loading encountered under the current mining conditions. In addition to the obse^ation and ｜ll^trumentation, numerical modeling was per- formed to evaluate the stress condi~!ons. Both the LaModel 3.0 and Rocscience Phase 2 programs were used to evaluate these four panels, The results of both models indicated a drastic reduction in the vertical stresses experienced in these panels due to the full extraction mining in overlying seams when compared to the full overburden load. Both models showed a higher level of stress associated with the outside entries of the panels. These results agree quite well with the observations and instrumentation studies performed at the mine. These efforts provided two overarching conclusions concerning R ＆ P retreat mine planning and execution. The first was that there are four areas that should not be overlooked during R ＆ P retreat mining： topographic relief, multiple^seaPa stress relief, stress concentrations near the gob edge, and geologic changes in the immediate roof. The second is that in order to successfully retreat an R ＆ P panel, a three-phased approach to the design and analysis of the panel should be conducted： the planning phase, evaluation phase, and monitoring phase.

Altered profiles of nuclear matrix proteins during the differentiation of human gastric mucous adenocarcinoma MGc80-3 cells

AIM： To find and identify specific nuclear matrix proteins associated with proliferation and differentiation of carcinoma cells, which will be potential markers for cancer diagnosis and targets in cancer therapy. METHODS： Nuclear matrix proteins were selectively extracted from MGcS0-3 cells treated with or without hexamethylamine bisacetamide （HMBA）, and subjected to 2-D gel electrophoresis. The resulted protein patterns were analyzed by Melanie software. Spots of nuclear matrix proteins differentially expressed were excised and subjected to in situ digestion with trypsin. Peptide masses were obtained by matrix-assisted laser-desorption/ ionization time of flight mass spectrometry （MALDI-TOFMS） analysis and submitted for database searching using Mascot tool. RESULTS： The MGc80-3 cells were induced into differentiation by HMBA. There were 22 protein spots which changed remarkably in the nuclear matrix, from differentiation of MGcS0-3 cells compared to control. Eleven of which were identified. Seven proteinsactin, prohibitin, porin 31HL, heterogeneous nuclear dbonucleoprotein A2/B1, vimentin, ATP synthase, and heat shock protein 60 were downregulated, whereas three proteins - heat shock protein gp96, heat shock protein 90-beta, and valosin-containing protein were upregulated, and the oxygen-regulated protein was only found in the differentiated MGc80-3 cells. CONCLUSION： The induced differentiation of carcinoma cells is accompanied by the changes of nuclear matrix proteins. Further characterization of those proteins will show the mechanism of cellular proliferation and differentiation, as well as cancer differentiation.

Hamatophyton from the Late Devonian of Anhui Province,South China and Evolution of Sphenophyllales

Well-preserved specimens of Hamatophyton verticillatum collected from the Upper Devonian （Famennian） Wutong Formation of Chaohu district, Anhui Province, South China, display more complete fertile axes in three orders and multiple divisions. Comparisons indicate that Hamatophyton possibly does not have palmate planate sterile leaves but hook-like linear ones with rare divisions. We propose seven definitive characters of Sphenophyllales： （1） completely whorled lateral organs; （2） sterile leaves; （3） strobili; （4） ＂sporangiophores＂ or stalks with reflexed tips bearing sporangia; （5） three- or four-ribbed primary xylem; （6） exarch maturation of primary xylem; and （7） secondary xylem. The Sphenophyllales probably originated from the Iridopteridales based on similarities in whorled lateral organs, ribbed primary xylem and peripheral protoxylem strands. In transition from Iridopteridales to Sphenophyllales, morphological changes involve partially whorled to completely whorled lateral organs, sterile ultimate appendages to leaves, and fertile ultimate appendages to ＂sporangiophores＂/stalks with bracts; anatomical modifications include configuration and maturation of primary xylem, and presence of secondary xylem.

Hypsometric properties of mountain landscape of Hunza River Basin of the Karakoram Himalaya

Within Karakoram Himalaya, Hunza River Basin(study area) is unique for a number of reasons: 1) potential impacts of highly concentrated highpitched mountains and glacial ice; 2) the glaciated portions have higher mean altitude as compared to other glaciated landscapes in the Karakoram; 3) this basin occupies varieties of both clean and debriscovered glaciers and/or ice. Therefore, it is imperative to understand the stability of topographic surface and potential implications of fluctuating glacial-ice causing variations in the movement of material from higher to lower elevations. This paper advocates landscape-level hypsometric investigations of glaciated landscape lies between 2280–7850 m elevation above sea level and non-glaciated landscape between 1461–7570 m. An attempt is made to understand intermediate elevations, which disguise the characteristics of glaciated hypsometries that are highly correlated with the Equilibrium Line Altitude(ELA). However, due to data scarcity for high altitude regions especially above 5000 m elevation, literature values for climatic conditions are used to create a relationship between hypsometry and variations in climate and ELA. The largest glaciated area(29.22%) between 5047 to 5555 m lies in the vertical regime of direct snow-accumulation zone and in the horizontal regime of net-accumulation zone(low velocity, net freezing, and no-sliding). In both landscapes, the hypsometric curves are ‘slow beginning' followed by ‘steep progress' and finally reaching a ‘plateau', reflecting the rapid altitudinal changes and the dominance of fluvial transport resulting in the denudation of land-dwelling and the transport of rock/debris from higher to lower altitudes. Reported slight differences in the average normalized bin altitudes against the cumulative normalized area between glaciated and non-glaciated landscapes are an indicator of slightly different land-forms and landform changes.

Coal matrix deformation characteristics in the process of carbon dioxide displacing different gas saturation coal-bed methane

It is fundamental that changes in coal reservoir permeability are researched, in particular, the accurate determination of variations in the coal matrix caused by CO2 replacing CH4 at different gas saturation conditions. Based on the surface free energy, the extended Langmuir isothermal adsorption model, combined with CO2 replacing CH4 in experimental trials, and calling on the more general principles and characteristics of the field, mathematical models describing the coal matrix as it undergoes different processes such as CO2 injection and desorption were established. Combined with laboratory data about CO2 replacement under different methane saturation conditions, a law governing the variations in coal matrix CO2 replacement under different methane gas saturation conditions was obtained. The results showed that： in the injection process, the coal matrix expansion rate caused by CO2 or CH4 was exponentially increased with the CO2 pressure increase, the expansion caused by CO2 was far greater than the expansion caused by CH4 in the desorption process, the coal matrix shrinkage caused by CO2 or CH4 was exponentially increased with the pressure decrease, the shrinkage caused by CO2 was larger than the shrinkage caused by CH4 under the same pressure and different gas saturation, the total shrinkage in the desorption process in the coal matrix was greater than the total expansion in the injection process. At higher gas saturations, the total coal matrix shrinkage volume exceeded the total expansion corresponding to pressure points higher in the desorption process.

Numerical Analysis of Adaptation-to-capacity Length for Fluvial Sediment Transport

Over the last several decades,various sediment transport capacity formulations have been used by geomorphologists and engineers to calculate fluvial morphological changes.However,it remains poorly understood if the adaptation to capacity could be fulfilled instantly in response to differing inflow discharges and sediment supplies,and thus if the calculation of morphological changes in rivers based on the assumed capacity status is fully justified.Here we present a numerical investigation on this issue.The distance required for sediment transport to adapt to capacity(i.e.,adaptation-to-capacity length) of both bed load and suspended sediment transport is computationally studied using a coupled shallow water hydrodynamic model,in line with varied inlet sediment concentrations.It is found that the adaptation-to-capacity length generally decreases as the Rouse number increases,irrespective of whether the inlet sediment concentration increases or reduces.For cases with vanishing inlet sediment concentration a unified relationship is found between the adaptation-to-capacity length and the Rouse number.Quantitatively,the adaptation-to-capacity length of bed load sediment is limited to tens of times of the flow depth,whilst that of suspended sediment increases substantially with decreasing Rouse number and can be up to hundreds of times of the flow depth.The present finding concurs that bed load sediment transport can adapt to capacity much more rapidly than suspended sediment transport,and it facilitates a quantitative criterion on which the applicability of bed load or suspended sediment transport capacity for natural rivers can be readily assessed.

Relationship between epithelial to mesenchymaltransition and chemoresistance of lung cancer

Objective： The aim of this study was to explore the correlation between epithelial to mesenchymal transition （EMT） and chemoresistance of non-small-cell lung cancer （NSCLC）. Methods： In vitro, the drug resistance index of cisplatin resistant lung adenocarcinoma cell line （A549/DDP） was detected by CCK-8 assay; the morphological change between A549/ DDP cells and lung adenocarcinoma cells （A549） was observed by phase contrast microscope; expression of EMT markers （including E-cadherin and vimentin） and resistance protein, excision repair cross-complementing 1 （ERCC1） was detected by immunocytochemistry. The expression of E-cadherin, vimentin and ERCC1 was investigated by immunohistochemistry in 120 cases of NSCLC, half of that were treated with pre-operative neoadjuvant chemotherapy （neoadjuvant chemotherapy group）, and the other underwent surgery alone （simple surgery group）. Results： There was a significant difference between the ICso （half maximal inhibitory concentration） of A549/DDP cells （5.20） and A549 cells （1.88） （P 〈 0.05）, and the drug resistance index of A549/DDP cells was 2.77. Compared with A549 cells, A549/DDP cells increased expression of ERCC1 （P 〈 0.05）. Moreover, A549/DDP cells showed morphological and phenotypic changes consistent with EMT： with spindle-shaped morphology, and decreased expression of E-cadherin and increased expression of vimentin. Immunohistochemistry showed significant positive correlation between the expression of ERCCI and vimentin （r = 0.496, 0.332, P 〈 0.05）, and significant negative correlation between the ERCCI and E-cadherin （r = -0.403, -0.295, P 〈 0.05） in neoadjuvant chemotherapy group and simple surgery group. In addition, compared with simple surgery group, the expression of ERCC1 （P = 0.003） and vimentin （P = 0.004） was significantly increased, and the expression of E-cadherin was decreased in neoadjuvant chemotherapy group （P = 0.032）. Cenclusion： A549/DDP cells acquired cisplatin-resistance and occurred EMT simultaneously; the phenomenon of chemoresistance and EMT was caused more easily in neoadjuvant chemotherapy group. As such, we further confirmed the close correlation between chemoresistance and EMT of NSCLC, and provided theoretical basis for the targeting therapy with EMT regulatory factor for chemoresistant NSCLC patients.

Linear and Nonlinear Optical Properties of Spherical Quantum Dots:Effects of Hydrogenic Impurity and Conduction Band Non-Parabolicity

Simultaneous effects of an on-center hydrogenic impurity and band edge non-parabolicity on intersubband optical absorption coefficients and refractive index changes of a typical GaAs/AlxGa1-xAs spherical quantum dot are theoretically investigated, using the Luttinger-Kohn effective mass equation. So, electronic structure and optical properties of the system are studied by means of the matrix diagonalization technique and compact density matrix approach, respectively. Finally, effects of an impurity, band edge non-parabolicity, incident light intensity and the dot size on the linear, the third-order nonlinear and the total optical absorption coemcients and refractive index changes are investigated. Our results indicate that, the magnitudes of these optical quantities increase and their peaks shift to higher energies as the influences of the impurity and the band edge non-parabolicity are considered. Moreover, incident light intensity and the dot size have considerable effects on the optical absorption coefficients and refractive index changes.