针对通用目标检测场景下,现有单阶段无锚检测器识别精度低、识别困难等问题,提出一种基于改进变焦网络VFNet(VarifocalNet)的高精度目标检测算法。首先,利用循环层聚合网络(RLANet)替换VFNet用于特征提取的主干网络ResNet,循环残差连接...针对通用目标检测场景下,现有单阶段无锚检测器识别精度低、识别困难等问题,提出一种基于改进变焦网络VFNet(VarifocalNet)的高精度目标检测算法。首先,利用循环层聚合网络(RLANet)替换VFNet用于特征提取的主干网络ResNet,循环残差连接操作将前层特征汇入后续网络层中提升特征的表征能力;其次,通过带有特征对齐卷积操作的特征金字塔网络(FPN)替换原始的特征融合网络,利用可变形卷积操作在FPN上下层融合过程中实现特征对齐并优化特征表征能力;最后,使用聚焦-全局蒸馏(FGD)算法进一步提升小规模算法的检测性能。在COCO(Common Objects in Context)2017数据集上进行的评估实验结果表明,在相同训练条件下,改进后的以RLANet-50为主干的算法的均值平均精度(mAP)可以达到45.9%,与VFNet算法相比提升了4.3个百分点,而改进后的算法参数量为36.67×10^(6),与VFNet相比仅高了4×10^(6)。可见,改进后的VFNet算法在提升检测精度的同时稍微增加了参数量,说明该算法可以满足目标检测的轻量化及高精度需求。展开更多
为了实现养殖场环境下羊只个体的有效识别及对羊只姿态进行迅速判断,针对现有模型识别精度差,效率低等问题,基于自建数据集,提出基于YOLOv3的改进模型。在主干网络darknet53中,首先引入通道注意力模块压缩激励网络,增强网络对重要通道...为了实现养殖场环境下羊只个体的有效识别及对羊只姿态进行迅速判断,针对现有模型识别精度差,效率低等问题,基于自建数据集,提出基于YOLOv3的改进模型。在主干网络darknet53中,首先引入通道注意力模块压缩激励网络,增强网络对重要通道的关注度,提高网络检测精度;其次,将网络中的部分残差模块用循环特征移位聚合模块替代,以提高检测速度和检测精度;采用余弦退火动态学习率替代原有学习率,在训练过程中进行动态微调,使网络能轻松跳出局部最优解。实验结果表明:在羊只检测与姿态识别任务中,YOLOv3-SE-RE算法的平均精度(mean Average Precision,mAP)比原始YOLOv3算法的mAP提升了9.98%,同时检测速度也得到提升。展开更多
Vibrio anguillarum is an important bacterial pathogen of aquatic organisms and a significant problem in aquatic farming. The rapid detection and identification of V. anguillarum, and other pathogens that infect marine...Vibrio anguillarum is an important bacterial pathogen of aquatic organisms and a significant problem in aquatic farming. The rapid detection and identification of V. anguillarum, and other pathogens that infect marine organisms, is crucial to effective disease management. In this study, we developed a loop-mediated amplification (LAMP) assay to detect V. anguillarum in an hour in a single tube without the need for thermal cycling. Conserved regions of the metalloproteinase (empA) gene of V. anguillarum served as the targets for primer design. A fragment of the empA gene was amplified at 65℃ in the presence of the primer mixture and Bst DNA polymerase. In the optimized LAMP assay, 6.7 pg of V. anguillarum DNA could be detected. Six strains of V. anguillarum and 17 strains of non-V, anguillarum bacteria were used in this study to evaluate the species specificity of the primers. The six V. anguillarum strains gave a positive result in the LAMP assay. This method was also validated in V. anguillarum-infected fish. This LAMP method is more sensitive than PCR in the detection of V. anguillarum and shows good species specificity. The LAMP assay is therefore an effective method for the quick detection of V. anguillarum both in the laboratory and in the field.展开更多
An investigation was performed on the suitability of carbon materials, metallic lead and its alloys as substrates for zinc negative electrode in acid PbO2-Zn single flow batteries. The zinc deposition process was carr...An investigation was performed on the suitability of carbon materials, metallic lead and its alloys as substrates for zinc negative electrode in acid PbO2-Zn single flow batteries. The zinc deposition process was carried out in the mediumofl mol.L 1H2SO4 at room temperature. No maximum current appears on the potentiostatic current transients for the zinc deposition on lead and its alloys. With increasing overpotential, the progressive nucleation turns to be a 3D-instantaneous nucleation process for the resin-graphite composite. Hydrogen evolution on the graphite composite is effectively suppressed with the doping of a polymer resin. The hydrogen evolution reaction on the lead is relatively weak, while on the lead alloys, it becomes serious to a certain degree. Although the ex- change current density of zinc deposition and dissolution process on the graphite composite is relatively low, the zinc corrosion is weakened to a great extent. With the increase of deposition time, zinc deposits are more compact. The cyclings of zinc galvanostatic charge-discharge on the graphite composite provide more than 90% of coulombic and 80% of energy efficiencies, and exhibit superior cycling stability during the first 10 cycles.展开更多
Ionic liquid like 1-ethyl-3-methylimidazolium bromine ([EMIM]Br) has been used as electrolyte for the electropolymerization of O-phenylenediamine at glassy carbon electrode by cyclic voltammetry. It is found that poly...Ionic liquid like 1-ethyl-3-methylimidazolium bromine ([EMIM]Br) has been used as electrolyte for the electropolymerization of O-phenylenediamine at glassy carbon electrode by cyclic voltammetry. It is found that poly (O-phenylenediamine) film modified electrode has favorable electrochemical activity in acid solution.展开更多
The electro-polymerization behavior of aniline in reverse(W/O) microemulsion was investigated. The experiment results show that the cyclic voltammetry polymerization behavior of aniline in W/O microemulsion is differe...The electro-polymerization behavior of aniline in reverse(W/O) microemulsion was investigated. The experiment results show that the cyclic voltammetry polymerization behavior of aniline in W/O microemulsion is different from that in aqueous solution remarkably. With the increase of scan cycle, the oxidation potential shifts positively and the reduction potential shifts negatively, i.e., the redox potential difference increases. H+ apparent concentration affects the aniline polymerization evidently. When H+ concentration is lower than 0.08 mol/L, the electro-polymerization of aniline is difficult. With the increase of H+ concentration, the polymerization current of aniline increases gradually. Only when H+ concentration is high enough(0.5 mol/L), aniline can be well electro-polymerized. Moreover, under the same condition, the aniline polymerization current in W/O microemulsion is higher than that in aqueous solution. The scanning electron microscopy image shows that the deposited polyaniline(PANI) has uniform fiber morphology with diameter of about 100 nm. Further study result suggests that the electrochemical activity of the PANI in HCl is similar to that of the PANI prepared in aqueous solution.展开更多
Soil aggregate stability,an important index of the physical characteristics of a soil,can provide a good indication of a soil’s erodibility,and deserves special consideration in regions with cold climate.The objectiv...Soil aggregate stability,an important index of the physical characteristics of a soil,can provide a good indication of a soil’s erodibility,and deserves special consideration in regions with cold climate.The objective of this study was to study the effect of freeze-thaw on soil water-stable aggregates in the black soil region of Northeast China.Samples of a typical black soil in the region were collected to measure water-stable aggregates after freeze-thaw under different conditions(i.e.,initial moisture contents,freezethaw cycles and freezing temperatures)by wet-sieving into eight particle size groups(>10,10–6,6–5,5–3,3–2,2–1,1–0.5,and0.5–0.25 mm).Freeze-thaw had the most effect on aggregate stability when the samples had an initial moisture content of 400 g kg-1.The water-stable aggregates of the four larger particle size groups(>5,5–3,3–2,and 2–1 mm)reached a peak stability value,but those of the two smaller particle size groups(1–0.5 and 0.5–0.25 mm)reached a minimum value when the soil moisture content was 400 g kg-1.Water-stable aggregates of the four larger particle size groups decreased while those of the two smaller particle size groups increased with the increase of freeze-thaw cycles.As temperatures fell,the water-stable aggregates of the four larger particle size groups decreased while those of the two smaller particle size groups increased.展开更多
Conductive polymer coatings can boost the power storage capacity of lithiumsulfur batteries. We report here on the design and preparation--by combining a facile and green chemical deposition method with an oxidative p...Conductive polymer coatings can boost the power storage capacity of lithiumsulfur batteries. We report here on the design and preparation--by combining a facile and green chemical deposition method with an oxidative polymerization approach--of polyaniline (PANI)-modified cetyltrimethylammonium bromide (CTAB)-graphene oxide (GO)-sulfur (S) nanocomposites with significantly enhanced performance in lithium-sulfur batteries. Such conductive polymer modified CTAB-GO-S nanocomposites as sulfur cathode materials can deliver high specific discharge capacities and long-term cycling performance, i.e., -970 mAh-g-1 at 0.2 C and -715 mAh-g-1 after 300 cycles, -820 mAh.g-1 at 0.5 C and -670 mAh.g-1 after 500 cycles, -770 mAh.K at 1 C and -570 mAh.g-~ after 500 cycles. The capacity decay was as low as 0.036% per cycle at 0.5 C, and 0.051% per cycle at 1 C. Under the same condition, batteries using PANI-modified CTAB-GO-S as cathodes exhibited higher specific capacity and higher average coulombic efficiency compared with CTAB-decorated GO-S and GO--S nano- composites. The improved performance can be attributed to the lower charge transfer resistance and the alleviated dissolution of polysulfides in the PANI- modified CTAB-GO-S cathodes.展开更多
文摘针对通用目标检测场景下,现有单阶段无锚检测器识别精度低、识别困难等问题,提出一种基于改进变焦网络VFNet(VarifocalNet)的高精度目标检测算法。首先,利用循环层聚合网络(RLANet)替换VFNet用于特征提取的主干网络ResNet,循环残差连接操作将前层特征汇入后续网络层中提升特征的表征能力;其次,通过带有特征对齐卷积操作的特征金字塔网络(FPN)替换原始的特征融合网络,利用可变形卷积操作在FPN上下层融合过程中实现特征对齐并优化特征表征能力;最后,使用聚焦-全局蒸馏(FGD)算法进一步提升小规模算法的检测性能。在COCO(Common Objects in Context)2017数据集上进行的评估实验结果表明,在相同训练条件下,改进后的以RLANet-50为主干的算法的均值平均精度(mAP)可以达到45.9%,与VFNet算法相比提升了4.3个百分点,而改进后的算法参数量为36.67×10^(6),与VFNet相比仅高了4×10^(6)。可见,改进后的VFNet算法在提升检测精度的同时稍微增加了参数量,说明该算法可以满足目标检测的轻量化及高精度需求。
文摘为了实现养殖场环境下羊只个体的有效识别及对羊只姿态进行迅速判断,针对现有模型识别精度差,效率低等问题,基于自建数据集,提出基于YOLOv3的改进模型。在主干网络darknet53中,首先引入通道注意力模块压缩激励网络,增强网络对重要通道的关注度,提高网络检测精度;其次,将网络中的部分残差模块用循环特征移位聚合模块替代,以提高检测速度和检测精度;采用余弦退火动态学习率替代原有学习率,在训练过程中进行动态微调,使网络能轻松跳出局部最优解。实验结果表明:在羊只检测与姿态识别任务中,YOLOv3-SE-RE算法的平均精度(mean Average Precision,mAP)比原始YOLOv3算法的mAP提升了9.98%,同时检测速度也得到提升。
基金Supported by the National Basic Research Program of China (973 Program) (No. 2006CB101804)the General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China (No. 2007IK167)National Department Public Benefit Research Foundation (No. 200803012)
文摘Vibrio anguillarum is an important bacterial pathogen of aquatic organisms and a significant problem in aquatic farming. The rapid detection and identification of V. anguillarum, and other pathogens that infect marine organisms, is crucial to effective disease management. In this study, we developed a loop-mediated amplification (LAMP) assay to detect V. anguillarum in an hour in a single tube without the need for thermal cycling. Conserved regions of the metalloproteinase (empA) gene of V. anguillarum served as the targets for primer design. A fragment of the empA gene was amplified at 65℃ in the presence of the primer mixture and Bst DNA polymerase. In the optimized LAMP assay, 6.7 pg of V. anguillarum DNA could be detected. Six strains of V. anguillarum and 17 strains of non-V, anguillarum bacteria were used in this study to evaluate the species specificity of the primers. The six V. anguillarum strains gave a positive result in the LAMP assay. This method was also validated in V. anguillarum-infected fish. This LAMP method is more sensitive than PCR in the detection of V. anguillarum and shows good species specificity. The LAMP assay is therefore an effective method for the quick detection of V. anguillarum both in the laboratory and in the field.
基金Supported by the National Basic Research Program(973 Program)of China(2010CB227201)the State Key Program of National Natural Science of China(21236003)+2 种基金the National Natural Science Foundation of China(21476022)the Fundamental Research Funds for the Central Universities(JD1515 and YS1406)Beijing Higher Education Young Elite Teacher Project(YETP0509)
文摘An investigation was performed on the suitability of carbon materials, metallic lead and its alloys as substrates for zinc negative electrode in acid PbO2-Zn single flow batteries. The zinc deposition process was carried out in the mediumofl mol.L 1H2SO4 at room temperature. No maximum current appears on the potentiostatic current transients for the zinc deposition on lead and its alloys. With increasing overpotential, the progressive nucleation turns to be a 3D-instantaneous nucleation process for the resin-graphite composite. Hydrogen evolution on the graphite composite is effectively suppressed with the doping of a polymer resin. The hydrogen evolution reaction on the lead is relatively weak, while on the lead alloys, it becomes serious to a certain degree. Although the ex- change current density of zinc deposition and dissolution process on the graphite composite is relatively low, the zinc corrosion is weakened to a great extent. With the increase of deposition time, zinc deposits are more compact. The cyclings of zinc galvanostatic charge-discharge on the graphite composite provide more than 90% of coulombic and 80% of energy efficiencies, and exhibit superior cycling stability during the first 10 cycles.
文摘Ionic liquid like 1-ethyl-3-methylimidazolium bromine ([EMIM]Br) has been used as electrolyte for the electropolymerization of O-phenylenediamine at glassy carbon electrode by cyclic voltammetry. It is found that poly (O-phenylenediamine) film modified electrode has favorable electrochemical activity in acid solution.
基金Projects(51071067,21271069,20673036,J1210040,50473022) supported by National Natural Science Foundation of ChinaProject(2013GK3015) supported by the Science and Technology Program of Hunan Province,China
文摘The electro-polymerization behavior of aniline in reverse(W/O) microemulsion was investigated. The experiment results show that the cyclic voltammetry polymerization behavior of aniline in W/O microemulsion is different from that in aqueous solution remarkably. With the increase of scan cycle, the oxidation potential shifts positively and the reduction potential shifts negatively, i.e., the redox potential difference increases. H+ apparent concentration affects the aniline polymerization evidently. When H+ concentration is lower than 0.08 mol/L, the electro-polymerization of aniline is difficult. With the increase of H+ concentration, the polymerization current of aniline increases gradually. Only when H+ concentration is high enough(0.5 mol/L), aniline can be well electro-polymerized. Moreover, under the same condition, the aniline polymerization current in W/O microemulsion is higher than that in aqueous solution. The scanning electron microscopy image shows that the deposited polyaniline(PANI) has uniform fiber morphology with diameter of about 100 nm. Further study result suggests that the electrochemical activity of the PANI in HCl is similar to that of the PANI prepared in aqueous solution.
基金Supported by the National Natural Science Foundation of China(Nos.41071183 and 40601054)
文摘Soil aggregate stability,an important index of the physical characteristics of a soil,can provide a good indication of a soil’s erodibility,and deserves special consideration in regions with cold climate.The objective of this study was to study the effect of freeze-thaw on soil water-stable aggregates in the black soil region of Northeast China.Samples of a typical black soil in the region were collected to measure water-stable aggregates after freeze-thaw under different conditions(i.e.,initial moisture contents,freezethaw cycles and freezing temperatures)by wet-sieving into eight particle size groups(>10,10–6,6–5,5–3,3–2,2–1,1–0.5,and0.5–0.25 mm).Freeze-thaw had the most effect on aggregate stability when the samples had an initial moisture content of 400 g kg-1.The water-stable aggregates of the four larger particle size groups(>5,5–3,3–2,and 2–1 mm)reached a peak stability value,but those of the two smaller particle size groups(1–0.5 and 0.5–0.25 mm)reached a minimum value when the soil moisture content was 400 g kg-1.Water-stable aggregates of the four larger particle size groups decreased while those of the two smaller particle size groups increased with the increase of freeze-thaw cycles.As temperatures fell,the water-stable aggregates of the four larger particle size groups decreased while those of the two smaller particle size groups increased.
文摘Conductive polymer coatings can boost the power storage capacity of lithiumsulfur batteries. We report here on the design and preparation--by combining a facile and green chemical deposition method with an oxidative polymerization approach--of polyaniline (PANI)-modified cetyltrimethylammonium bromide (CTAB)-graphene oxide (GO)-sulfur (S) nanocomposites with significantly enhanced performance in lithium-sulfur batteries. Such conductive polymer modified CTAB-GO-S nanocomposites as sulfur cathode materials can deliver high specific discharge capacities and long-term cycling performance, i.e., -970 mAh-g-1 at 0.2 C and -715 mAh-g-1 after 300 cycles, -820 mAh.g-1 at 0.5 C and -670 mAh.g-1 after 500 cycles, -770 mAh.K at 1 C and -570 mAh.g-~ after 500 cycles. The capacity decay was as low as 0.036% per cycle at 0.5 C, and 0.051% per cycle at 1 C. Under the same condition, batteries using PANI-modified CTAB-GO-S as cathodes exhibited higher specific capacity and higher average coulombic efficiency compared with CTAB-decorated GO-S and GO--S nano- composites. The improved performance can be attributed to the lower charge transfer resistance and the alleviated dissolution of polysulfides in the PANI- modified CTAB-GO-S cathodes.