AIM： To investigate the effect of Tripterygium hyp-oglaucum Hutch （THH） on the assembly and disassembly process of tubulin and its possible mode of action.METHODS： In vitro porcine brain tubulin assembly assay was...AIM： To investigate the effect of Tripterygium hyp-oglaucum Hutch （THH） on the assembly and disassembly process of tubulin and its possible mode of action.METHODS： In vitro porcine brain tubulin assembly assay was employed to analyze the inhibitory effects of THH at different concentrations （0.05 μg/L, 0.07 μg/L, 0.09μg/L）. Colchicine （0.0025 mmol/L, 0.0050 mmol/L, 0.0075 mmol/L） was used as a positive control.RESULTS： THH could significantly inhibit the assembly of isolated porcine brain tubulin at all tested concentrations.CONCLUSION： THH is capable of inducing aneuploidy in mammals via tubulin polymerization inhibition pathway and may pose a genetic risk to human beings.展开更多
The effects of Chinese medicines on micro-circulation and blood flow velocity in arteries of the lower limbs were observed in 33 patients with arteriosclerotic obliteration(ASO).The results showed that the integral va...The effects of Chinese medicines on micro-circulation and blood flow velocity in arteries of the lower limbs were observed in 33 patients with arteriosclerotic obliteration(ASO).The results showed that the integral values of micro-circulation after treatment were obviously higher than those before treatment (P<0.05 or P<0.01).Blood flow velocity in arteries of the lower limbs determined with the color Doppler ultrasound detecting method after treatment were also obviously higher than those before treatment (P<0.05 or P<0.01).In tbis series of 33 ASO patients,the markedly effective rate was 36.36%,and the total effective rate was 63.64%.The observation indicates that the recipe prescribed according to the principle of supplementing qi and activating blood circulation can effectively improve micro-circulation in ASO patients,and accelerate blood flow in arteries of the lower limbs.展开更多
Chinese Angelica is sweet and pungent in flavour and warm in property. It has the effect of tonifying blood and activating blood circulation and is frequently applied for such gynecological diseases as irregular menst...Chinese Angelica is sweet and pungent in flavour and warm in property. It has the effect of tonifying blood and activating blood circulation and is frequently applied for such gynecological diseases as irregular menstruation and weak constitution after delivery. Therefore, Chinese Angelica has also the name of 'sacred medicament in gynecology'.展开更多
After the microbiology based on Pasteur's method and polymerase chain reaction(PCR), the diagnosis company named Dendris has proposed a third-generation of diagnosis enabling the search of a broad range of pathoge...After the microbiology based on Pasteur's method and polymerase chain reaction(PCR), the diagnosis company named Dendris has proposed a third-generation of diagnosis enabling the search of a broad range of pathogens with strong sensitivity and specificity. This extraordinary profile was possible thanks to the use of phosphorus dendrimers for which various techniques of deposition on a given support were investigated and described and analyzed in this report.展开更多
Symbionts are microorganisms residing in multicellular hosts(e.g., plants and animals), and they have been witnessed to be a rich source of diverse functional molecules. This review describes structures and biological...Symbionts are microorganisms residing in multicellular hosts(e.g., plants and animals), and they have been witnessed to be a rich source of diverse functional molecules. This review describes structures and biological activities of symbiont-derived secondary metabolites commonly referred to as "natural products", and highlights that symbiotic microbes represent an underexplored reservoir of natural products with unique scaffolds and promising significance in managing human healthcare and agricultural production.展开更多
The rapid developments of genomics and proteomics have driven the demand for multiplex and high throughput analysis of large numbers of biomolecules in the fields of medical diagnostics, drug discovery, and environmen...The rapid developments of genomics and proteomics have driven the demand for multiplex and high throughput analysis of large numbers of biomolecules in the fields of medical diagnostics, drug discovery, and environmental monitoring. Encoding the biomolecular binding events is the key technique to fulfill this demand, in which microparticles play the most important roles. This review outlines the development of multiplex and high throughput biodetections, and highlights the most recent advances in the field of encoding microparticles, together with problems that need to be resolved.展开更多
Background:There is increasing public discussion about the escalating cost of healthcare in America.There are no published data regarding the contribution of individual surgeons’choices on the cost of uncomplicated m...Background:There is increasing public discussion about the escalating cost of healthcare in America.There are no published data regarding the contribution of individual surgeons’choices on the cost of uncomplicated minimally invasive colectomy.Methods:A review of a hospital cost-accounting database of the direct costs related to the index operation and postoperative care of all patients who underwent elective minimally invasive segmental colectomy over a 1-year period was performed.Results:A total of 111 cases were enrolled in this study,18 of which were performed robotically.The average direct cost after minimally invasive colectomy was$5536.The cost of robotic colectomy was 53%greater than laparoscopic($7806 vs$5096,p<0.001).There was no statistically significant difference in overall costs among laparoscopic cases performed by three surgeons($5099 vs$5108 vs$5055,p¼0.987).Average operating room supply costs among the three surgeons were$1236,$1105 and$1030,respectively(p¼0.067),with a standard deviation of$328(6.4%of overall cost).Conclusions:No significant difference in overall costs between surgeons was demonstrated despite varied training,experience levels and operative techniques.Total costs are relatively institutionally fixed and minimally influenced by variations in individual surgeon preferences.展开更多
Micro gas chromatography(μGC) has been continuously gaining attention since the last century owing to multiple favorable characteristics, such as its small size, low power consumption and minimal production and maint...Micro gas chromatography(μGC) has been continuously gaining attention since the last century owing to multiple favorable characteristics, such as its small size, low power consumption and minimal production and maintenance costs.μGC has the potential to provide practical solutions to emerging analytical challenges in security, health,and environment. In this review, we summarize recent advances in micro detectors for μGC, including the study of the miniaturization of conventional detectors and the development of novel detectors for μGC chromatography.展开更多
基金Supported by the National Natural Science Foundation of China,No,30560061 the International Scientific Cooperation Project of Yunnan Province.
文摘AIM： To investigate the effect of Tripterygium hyp-oglaucum Hutch （THH） on the assembly and disassembly process of tubulin and its possible mode of action.METHODS： In vitro porcine brain tubulin assembly assay was employed to analyze the inhibitory effects of THH at different concentrations （0.05 μg/L, 0.07 μg/L, 0.09μg/L）. Colchicine （0.0025 mmol/L, 0.0050 mmol/L, 0.0075 mmol/L） was used as a positive control.RESULTS： THH could significantly inhibit the assembly of isolated porcine brain tubulin at all tested concentrations.CONCLUSION： THH is capable of inducing aneuploidy in mammals via tubulin polymerization inhibition pathway and may pose a genetic risk to human beings.
文摘The effects of Chinese medicines on micro-circulation and blood flow velocity in arteries of the lower limbs were observed in 33 patients with arteriosclerotic obliteration(ASO).The results showed that the integral values of micro-circulation after treatment were obviously higher than those before treatment (P<0.05 or P<0.01).Blood flow velocity in arteries of the lower limbs determined with the color Doppler ultrasound detecting method after treatment were also obviously higher than those before treatment (P<0.05 or P<0.01).In tbis series of 33 ASO patients,the markedly effective rate was 36.36%,and the total effective rate was 63.64%.The observation indicates that the recipe prescribed according to the principle of supplementing qi and activating blood circulation can effectively improve micro-circulation in ASO patients,and accelerate blood flow in arteries of the lower limbs.
文摘Chinese Angelica is sweet and pungent in flavour and warm in property. It has the effect of tonifying blood and activating blood circulation and is frequently applied for such gynecological diseases as irregular menstruation and weak constitution after delivery. Therefore, Chinese Angelica has also the name of 'sacred medicament in gynecology'.
基金supported by the National Research Agency (Agence Nationale pour la Recherche), “BIOTECHNOLOGIES” program (ANR 2010 BIOT 004 06: Project INNODIAG to JMF)Region Midi Pyrénées (06001324 & 07006292) to RF and JMFCNRS (JPM, AMC)
文摘After the microbiology based on Pasteur's method and polymerase chain reaction(PCR), the diagnosis company named Dendris has proposed a third-generation of diagnosis enabling the search of a broad range of pathogens with strong sensitivity and specificity. This extraordinary profile was possible thanks to the use of phosphorus dendrimers for which various techniques of deposition on a given support were investigated and described and analyzed in this report.
基金supported by the National High Technology Research and Development Program of China(2013AA092901)the National Natural Science Foundation of China(81121062,21132004,81421091)
文摘Symbionts are microorganisms residing in multicellular hosts(e.g., plants and animals), and they have been witnessed to be a rich source of diverse functional molecules. This review describes structures and biological activities of symbiont-derived secondary metabolites commonly referred to as "natural products", and highlights that symbiotic microbes represent an underexplored reservoir of natural products with unique scaffolds and promising significance in managing human healthcare and agricultural production.
基金support of Grant for Teaching and Research of Excellent Young Teachers in Southeast University333 Talent Project Foundation and the Qing Lan Project of Jiangsu Province+2 种基金Jiangsu Science and Technology Department (BE2009148 & Bk2008318)the National Natural Science Foundation of China (50925309, 20703010 & 21073033)the Scientific Research Foundation of the Graduate School of Southeast University
文摘The rapid developments of genomics and proteomics have driven the demand for multiplex and high throughput analysis of large numbers of biomolecules in the fields of medical diagnostics, drug discovery, and environmental monitoring. Encoding the biomolecular binding events is the key technique to fulfill this demand, in which microparticles play the most important roles. This review outlines the development of multiplex and high throughput biodetections, and highlights the most recent advances in the field of encoding microparticles, together with problems that need to be resolved.
文摘Background:There is increasing public discussion about the escalating cost of healthcare in America.There are no published data regarding the contribution of individual surgeons’choices on the cost of uncomplicated minimally invasive colectomy.Methods:A review of a hospital cost-accounting database of the direct costs related to the index operation and postoperative care of all patients who underwent elective minimally invasive segmental colectomy over a 1-year period was performed.Results:A total of 111 cases were enrolled in this study,18 of which were performed robotically.The average direct cost after minimally invasive colectomy was$5536.The cost of robotic colectomy was 53%greater than laparoscopic($7806 vs$5096,p<0.001).There was no statistically significant difference in overall costs among laparoscopic cases performed by three surgeons($5099 vs$5108 vs$5055,p¼0.987).Average operating room supply costs among the three surgeons were$1236,$1105 and$1030,respectively(p¼0.067),with a standard deviation of$328(6.4%of overall cost).Conclusions:No significant difference in overall costs between surgeons was demonstrated despite varied training,experience levels and operative techniques.Total costs are relatively institutionally fixed and minimally influenced by variations in individual surgeon preferences.
基金the financial support from the National Natural Science Foundation of China (61674114, 91743110 and 21861132001)the National Key R&D Program of China (2017YFF0204600)+2 种基金Tianjin Applied Basic Research and Advanced Technology (17JCJQJC43600)the Foundation for Talent Scientists of Nanchang Institute for Micro-technology of Tianjin Universitythe 111 Project (B07014 and B12015)
文摘Micro gas chromatography(μGC) has been continuously gaining attention since the last century owing to multiple favorable characteristics, such as its small size, low power consumption and minimal production and maintenance costs.μGC has the potential to provide practical solutions to emerging analytical challenges in security, health,and environment. In this review, we summarize recent advances in micro detectors for μGC, including the study of the miniaturization of conventional detectors and the development of novel detectors for μGC chromatography.
