In the paper, DNA fingerprinting patterns of common vole (Microtus arvalis) were detected with HindIII, HinfI and microsatellite probe (GTG) 5 .The results showed that legible DNA fingerprints were not produced with H...In the paper, DNA fingerprinting patterns of common vole (Microtus arvalis) were detected with HindIII, HinfI and microsatellite probe (GTG) 5 .The results showed that legible DNA fingerprints were not produced with HindIII and probe (GTG) 5, however HinfI and probe (GTG)5 produced abundantly informative DNA fingerprints. DNA fingerprints were individual-specific and represented higher polymorphism in number, intensity and distribution bands among individuals. The band-sharing rates (BSR) between individuals were determined by the DNA fingerprints. The average band numbers and average band-sharing rates in fragment length region (FLR) of >2.3 kb were 29.625±5.012 and 0.388±0.083 respectively. Correlative fingerprint-data with the population were calculated from average BSR. In this study, a technical basis is established for application of multilocus DNA fingerprinting in common vole.展开更多
多种小卫星探针和微卫星探针可以用来获得家畜家禽的 DNA 指纹图谱.象人的DNA 指纹图一样,畜禽的 DNA 指纹图也具有高度的变异性和稳定的遗传性.畜禽 DNA 指纹图已广泛地用于鉴定个体、测定品种(系)的遗传纯度和品种(系)间的遗传距离、...多种小卫星探针和微卫星探针可以用来获得家畜家禽的 DNA 指纹图谱.象人的DNA 指纹图一样,畜禽的 DNA 指纹图也具有高度的变异性和稳定的遗传性.畜禽 DNA 指纹图已广泛地用于鉴定个体、测定品种(系)的遗传纯度和品种(系)间的遗传距离、监测育种及遗传操作效应、寻找重要数量性状位点的遗传标记、开发单位点高变异探针等方面.展开更多
AIM: To detect the loss of heterozygosity (LOH) frequency of microsatellite sites D9s171, D9s1604 of p16 gene and expression of hMSH2 mRNA in various differentiated types of gastric cancer, adjacent cancer tissues and...AIM: To detect the loss of heterozygosity (LOH) frequency of microsatellite sites D9s171, D9s1604 of p16 gene and expression of hMSH2 mRNA in various differentiated types of gastric cancer, adjacent cancer tissues and normal gastric mucosa.METHODS: LOH was detected by polymerase chain reaction (PCR)-denaturing polyacrylamide gel electrophoresis-silver staining. The expression of hMSH2 mRNA was examined with in situ hybridization.RESULTS: The frequency rate of LOH was significantly higher in gastric cancers than that in adjacent cancer tissues (P=0.032). No significant difference was noted among various differentiated types and various clinical stages of gastric cancers. The significantly reduced expression of hMSH2mRNA positive signal cells exhibited in gastric cancers, in comparison with that in the adjacent cancer tissues and normal gastric mucosa, respectively (P=0.001). No significant difference was noted among various clinical stages of gastric cancers (P＞0.05). The difference of positive signal cells in poorly differentiated cancers and those in well and moderately differentiated cancers were significant (P＜0.001).CONCLUSION: The frequencies of LOH in two microsatellite sites, D9s171 and D9s1604, in p16 genome were associated with development of gastric cancer and no significant correlation was demonstrated between the LOH frequency and the cell differentiated types of tumor cells or clinical stages. There was a positive relationship between the expression of hMSH2 mRNA and the differentiated types of gastric cancer.展开更多
文摘In the paper, DNA fingerprinting patterns of common vole (Microtus arvalis) were detected with HindIII, HinfI and microsatellite probe (GTG) 5 .The results showed that legible DNA fingerprints were not produced with HindIII and probe (GTG) 5, however HinfI and probe (GTG)5 produced abundantly informative DNA fingerprints. DNA fingerprints were individual-specific and represented higher polymorphism in number, intensity and distribution bands among individuals. The band-sharing rates (BSR) between individuals were determined by the DNA fingerprints. The average band numbers and average band-sharing rates in fragment length region (FLR) of >2.3 kb were 29.625±5.012 and 0.388±0.083 respectively. Correlative fingerprint-data with the population were calculated from average BSR. In this study, a technical basis is established for application of multilocus DNA fingerprinting in common vole.
文摘多种小卫星探针和微卫星探针可以用来获得家畜家禽的 DNA 指纹图谱.象人的DNA 指纹图一样,畜禽的 DNA 指纹图也具有高度的变异性和稳定的遗传性.畜禽 DNA 指纹图已广泛地用于鉴定个体、测定品种(系)的遗传纯度和品种(系)间的遗传距离、监测育种及遗传操作效应、寻找重要数量性状位点的遗传标记、开发单位点高变异探针等方面.
基金the National Natural Science Foundation of China,No.39170440
文摘AIM: To detect the loss of heterozygosity (LOH) frequency of microsatellite sites D9s171, D9s1604 of p16 gene and expression of hMSH2 mRNA in various differentiated types of gastric cancer, adjacent cancer tissues and normal gastric mucosa.METHODS: LOH was detected by polymerase chain reaction (PCR)-denaturing polyacrylamide gel electrophoresis-silver staining. The expression of hMSH2 mRNA was examined with in situ hybridization.RESULTS: The frequency rate of LOH was significantly higher in gastric cancers than that in adjacent cancer tissues (P=0.032). No significant difference was noted among various differentiated types and various clinical stages of gastric cancers. The significantly reduced expression of hMSH2mRNA positive signal cells exhibited in gastric cancers, in comparison with that in the adjacent cancer tissues and normal gastric mucosa, respectively (P=0.001). No significant difference was noted among various clinical stages of gastric cancers (P＞0.05). The difference of positive signal cells in poorly differentiated cancers and those in well and moderately differentiated cancers were significant (P＜0.001).CONCLUSION: The frequencies of LOH in two microsatellite sites, D9s171 and D9s1604, in p16 genome were associated with development of gastric cancer and no significant correlation was demonstrated between the LOH frequency and the cell differentiated types of tumor cells or clinical stages. There was a positive relationship between the expression of hMSH2 mRNA and the differentiated types of gastric cancer.
