In the present study,the genetic diversity of one selected strain(Pujiang No.1),two domesticated populations(GA and HX)and four wild populations(LZ,YN,SS and JL)of blunt snout bream(Megalobrama amblycephala)was analyz...In the present study,the genetic diversity of one selected strain(Pujiang No.1),two domesticated populations(GA and HX)and four wild populations(LZ,YN,SS and JL)of blunt snout bream(Megalobrama amblycephala)was analyzed using 17microsatellite markers.The results showed that an average of 4.88-7.65 number of alleles(A);an average of 3.20-5.33 effective alleles(Ne);average observed heterozygosity(Ho)of 0.6985-0.9044;average expected heterozygosity(He)of 0.6501-0.7805;and the average polymorphism information content(PIC)at 0.5706-0.7226.Pairwise FST value between populations ranged from0.0307-0.1451,and Nei’s standard genetic distance between populations was 0.0938-0.4524.The expected heterozygosities in the domesticated populations(GA and HX)were significantly lower than those found in three wild populations(LZ,SS and JL),but no difference was detected when compared with the wild YN population.Likewise,no difference was found between the four wild populations or two domesticated populations.The expected heterozygosity in Pujiang No.1 was higher than the two domesticated populations and lower than the four wild populations.Regarding pairwise FST value between populations,permutation test P-values were significant between the GA,HX and PJ populations,but not between the four wild populations.These results showed that the expected heterozygosity in the selected strain of blunt snout bream,after seven generations of selective breeding,was lower than that of wild populations,but this strain retains higher levels of genetic diversity than domesticated populations.The genetic differences and differentiation amongst wild populations,domesticated populations and the genetically improved strain of blunt snout bream will provide important conservation criteria and guide the utilization of germplasm resources.展开更多
The expressed sequence tags (ESTs) of Japanese flounder, Paralichthys olivaceus, were selected from GenBank to iden-tify simple sequence repeats (SSRs) or microsatellites.A bioinformatic analysis of 11111 ESTs identif...The expressed sequence tags (ESTs) of Japanese flounder, Paralichthys olivaceus, were selected from GenBank to iden-tify simple sequence repeats (SSRs) or microsatellites.A bioinformatic analysis of 11111 ESTs identified 751 SSR-containing ESTs, including 440 dinucleotide, 254 trinucleotide, 53 tetranucleotide, 95 pentanucleotide and 40 hexanucleotide microsatellites respec-tively.The CA/TG and GA/TC repeats were the most abundant microsatellites.AT-rich types were predominant among trinucleotide and tetranucleotide microsatellites.PCR primers were designed to amplify 10 identified microsatellites loci.The PCR results from eight pairs of primers showed polymorphisms in wild populations.In 30 wild individuals, the mean observed and expected het-erozygosities of these 8 polymorphic SSRs were 0.71 and 0.83 respectively and the average PIC value was 0.8.These microsatellite markers should prove to be a useful addition to the microsatellite markers that are now available for this species.展开更多
基金Supported by the State Scientific Basic Research Platform Program( No. 2005DKA21101)China Postdoctoral Science Foundation(No. 20080430470)+7 种基金Natural Science Foundation of Jiangsu Prov-ince of China (BK2007556)the National high-tech R&D program(863 program)(No.2006AA10Z198)Key Projects in the NationalScience &Technology Pillar Program during the Eleventh Five-Year Plan Period(No.2006BAD13B08 )Support Foundation of Chinaduring the 11th Five-Year Plan Period (No. 2008BADB2B04)BasicNatural Science Foundation for Colleges and Universities in JiangsuProvince (NK051039)Jiangsu Government Scholarship for Over-seas Studies ProjectQing Lan Project of Colleges and UniversitiesJiangsu Provincethe New Century Talent Project of Yangzhou University in China~~
基金supported by the National Natural Science Foundation of China(30630051)Doctoral Research Initial Funding from Shanghai Ocean University(A-2400-11-0186)
文摘In the present study,the genetic diversity of one selected strain(Pujiang No.1),two domesticated populations(GA and HX)and four wild populations(LZ,YN,SS and JL)of blunt snout bream(Megalobrama amblycephala)was analyzed using 17microsatellite markers.The results showed that an average of 4.88-7.65 number of alleles(A);an average of 3.20-5.33 effective alleles(Ne);average observed heterozygosity(Ho)of 0.6985-0.9044;average expected heterozygosity(He)of 0.6501-0.7805;and the average polymorphism information content(PIC)at 0.5706-0.7226.Pairwise FST value between populations ranged from0.0307-0.1451,and Nei’s standard genetic distance between populations was 0.0938-0.4524.The expected heterozygosities in the domesticated populations(GA and HX)were significantly lower than those found in three wild populations(LZ,SS and JL),but no difference was detected when compared with the wild YN population.Likewise,no difference was found between the four wild populations or two domesticated populations.The expected heterozygosity in Pujiang No.1 was higher than the two domesticated populations and lower than the four wild populations.Regarding pairwise FST value between populations,permutation test P-values were significant between the GA,HX and PJ populations,but not between the four wild populations.These results showed that the expected heterozygosity in the selected strain of blunt snout bream,after seven generations of selective breeding,was lower than that of wild populations,but this strain retains higher levels of genetic diversity than domesticated populations.The genetic differences and differentiation amongst wild populations,domesticated populations and the genetically improved strain of blunt snout bream will provide important conservation criteria and guide the utilization of germplasm resources.
基金supported by the National High Technology Research and Development Program of China (Grant No.2006AA10A404)the National Natural Science Foundation of China (Grant No.30671624)
文摘The expressed sequence tags (ESTs) of Japanese flounder, Paralichthys olivaceus, were selected from GenBank to iden-tify simple sequence repeats (SSRs) or microsatellites.A bioinformatic analysis of 11111 ESTs identified 751 SSR-containing ESTs, including 440 dinucleotide, 254 trinucleotide, 53 tetranucleotide, 95 pentanucleotide and 40 hexanucleotide microsatellites respec-tively.The CA/TG and GA/TC repeats were the most abundant microsatellites.AT-rich types were predominant among trinucleotide and tetranucleotide microsatellites.PCR primers were designed to amplify 10 identified microsatellites loci.The PCR results from eight pairs of primers showed polymorphisms in wild populations.In 30 wild individuals, the mean observed and expected het-erozygosities of these 8 polymorphic SSRs were 0.71 and 0.83 respectively and the average PIC value was 0.8.These microsatellite markers should prove to be a useful addition to the microsatellite markers that are now available for this species.
