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一种应用于高通量生物检测的皮升级微反应池芯片 被引量:1
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作者 韩伟静 袁丽娜 +5 位作者 魏清泉 李运涛 任鲁风 周晓光 于军 俞育德 《中国科学:生命科学》 CSCD 北大核心 2013年第11期953-959,1-3,共7页
基于高通量皮升级微反应池的焦磷酸测序技术以其测序读长长的优势,在测序领域有着不可替代的位置.在高通量焦磷酸测序过程中,微反应池之间的光学串扰以及微反应池中的化学残留严重影响测序原始图像的信噪比,限制了测序的读长及准确性.... 基于高通量皮升级微反应池的焦磷酸测序技术以其测序读长长的优势,在测序领域有着不可替代的位置.在高通量焦磷酸测序过程中,微反应池之间的光学串扰以及微反应池中的化学残留严重影响测序原始图像的信噪比,限制了测序的读长及准确性.本研究通过在微反应池侧壁分别选择性蒸镀钛和铝金属膜,有效地将相邻微反应池之间的平均光学串扰率降低约一个数量级.此外,通过在微反应池表面蒸镀二氧化硅层,显著地改善了微反应池表面物理形貌,有效地减少了微反应池中的化学残留.表面蒸镀钛-二氧化硅的微反应池光学串扰低、化学残留少,可应用于高通量焦磷酸测序等相关领域. 展开更多
关键词 高通量分析 皮升级微反应池 表面镀膜
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新型微电极葡萄糖传感器 被引量:1
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作者 刘敬伟 边超 +2 位作者 韩泾鸿 夏善红 陈绍凤 《仪器仪表学报》 EI CAS CSCD 北大核心 2004年第z1期396-397,411,共3页
提出了一种新型的微电极葡萄糖传感器。以与IC兼容的硅作为基底材料,利用MEMS加工工艺,采用硅腐蚀及SU8微反应池方法制成了新型微电极传感器。与传统的电流型传感器相比,该传感器有较小的敏感面积(1mm×1mm),较低的检测下限(1×... 提出了一种新型的微电极葡萄糖传感器。以与IC兼容的硅作为基底材料,利用MEMS加工工艺,采用硅腐蚀及SU8微反应池方法制成了新型微电极传感器。与传统的电流型传感器相比,该传感器有较小的敏感面积(1mm×1mm),较低的检测下限(1×10-4M),较宽的检测范围(1×10-4~1×10-2M),较好的重现性与稳定性,以及易于与处理电路集成等优点。 展开更多
关键词 传感器 GOD 电极 微反应池 硅腐蚀 葡萄糖
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体硅加工微电极传感器研究
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作者 刘敬伟 边超 +3 位作者 韩泾鸿 陈绍凤 白强 夏善红 《仪器仪表学报》 EI CAS CSCD 北大核心 2004年第z3期144-147,共4页
应用新的体硅加工技术和新的电极表面修饰方法,提出了一种新型电流型微电极传感器。以与IC兼容的硅作为基底材料,利用体硅加工工艺,采用各向异性硅腐蚀及SU- 8微反应池方法制成了新型体硅加工电极。同时,铂化并聚合被首次用来作电极表... 应用新的体硅加工技术和新的电极表面修饰方法,提出了一种新型电流型微电极传感器。以与IC兼容的硅作为基底材料,利用体硅加工工艺,采用各向异性硅腐蚀及SU- 8微反应池方法制成了新型体硅加工电极。同时,铂化并聚合被首次用来作电极表面修饰。以葡萄糖检测为例,使用吡咯作为聚合材料,新传感器与传统的电流型传感器相比,有较小的敏感面积(1m m×1mm )、较大的敏感系数(39.6 4 0 n A.m M- 1 .m m- 2 )、较低的检测下限(1×10 - 4M)、较宽的检测范围(1×10 - 4~1×10 - 2 M)、较好的重现性(五次测量的RSD为3.2 % )与稳定性(温室保存一个月后活性保留95 %以上)、以及易于与处理电路集成等优点。 展开更多
关键词 传感器 电流型 电极 体硅加工 微反应池
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Hydrogen production performance of the non⁃platinum⁃based MoS_(2)/CuS cathode in microbial electrolytic cells
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作者 HAO Pingping LI Fangfang +5 位作者 WANG Yawen LI Houfen ZHANG Xiao LI Rui WANG Lei LIU Jianxin 《无机化学学报》 SCIE CAS CSCD 北大核心 2024年第9期1811-1824,共14页
MoS_(2)/CuS composite catalysts were successfully synthesized using a one-step hydrothermal method with sodium molybdate dihydrate,thiourea,oxalic acid,and copper nitrate trihydrate as raw materials.The hydrogen pro-d... MoS_(2)/CuS composite catalysts were successfully synthesized using a one-step hydrothermal method with sodium molybdate dihydrate,thiourea,oxalic acid,and copper nitrate trihydrate as raw materials.The hydrogen pro-duction performance of MoS_(2)/CuS prepared with different molar ratios of Mo to Cu precursors(n_(Mo)∶n_(Cu))as cathodic catalysts was investigated in the two-chamber microbial electrolytic cell(MEC).X-ray diffraction(XRD),X-ray pho-toelectron spectroscopy(XPS),scanning electron microscopy(SEM),transmission electron microscope(TEM),linear scanning voltammetry(LSV),electrochemical impedance analysis(EIS),and cyclic voltammetry(CV)were used to characterize the synthesized catalysts for testing and analyzing the hydrogen-producing performance.The results showed that the hydrogen evolution performance of MoS_(2)/CuS-20%(nMo∶nCu=5∶1)was better than that of platinum(Pt)mesh,and the hydrogen production rate of MoS_(2)/CuS-20%as a cathode in MEC was(0.2031±0.0237)m^(3)_(H_(2))·m^(-3)·d^(-1) for 72 h at an applied voltage of 0.8 V,which was slightly higher than that of Pt mesh of(0.1886±0.0134)m^(3)_(H_(2))·m^(-3)·d^(-1).The addition of a certain amount of CuS not only regulates the electron transfer ability of MoS_(2) but also increases the density of active sites. 展开更多
关键词 microbial electrolysis cell hydrogen evolution reaction MoS_(2)/CuS composite catalyst Pt mesh
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大型钢铁公司生产废水的处理与回用 被引量:14
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作者 范伟 吕军 +2 位作者 陆必云 苗利利 高慧东 《中国给水排水》 CAS CSCD 北大核心 2010年第6期80-84,共5页
为减少新水取水量,降低吨钢新水耗量,实现节能减排,江阴兴澄钢铁公司根据总体规划及生产发展用水要求建设了生产废水处理与回用工程。该工程处理规模为3.6×104m3/d,采用微涡旋反应沉淀池/双层过滤器工艺,运行成本约0.33元/m3,处理... 为减少新水取水量,降低吨钢新水耗量,实现节能减排,江阴兴澄钢铁公司根据总体规划及生产发展用水要求建设了生产废水处理与回用工程。该工程处理规模为3.6×104m3/d,采用微涡旋反应沉淀池/双层过滤器工艺,运行成本约0.33元/m3,处理出水与该厂净水处理站的出水混合后作循环水补水。介绍了该工程的工艺流程、主要构筑物的设计参数及特点。 展开更多
关键词 钢铁生产废水 涡旋反应沉淀 双层过滤器
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钢铁厂综合废水处理回用工程设计 被引量:12
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作者 欧阳丽 王晓明 《中国给水排水》 CAS CSCD 北大核心 2008年第20期73-76,共4页
采用微涡流反应沉淀池与V型滤池组合工艺处理某钢铁厂综合废水。运行表明,微涡流反应沉淀池处理效果良好,能有效延长滤池的工作周期,最终出水水质可同时满足厂区回用水标准和上海市《污水综合排放标准》(DB 31/199—1997)的一级标准。
关键词 涡流反应沉淀 V型滤 废水再生回用
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A system combining microbial fuel cell with photobioreactor for continuous domestic wastewater treatment and bioelectricity generation 被引量:9
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作者 蒋海明 罗生军 +2 位作者 师晓爽 戴萌 郭荣波 《Journal of Central South University》 SCIE EI CAS 2013年第2期488-494,共7页
A coupled system consisting of an upflow membrane-less microbial fuel cell (upflow ML-MFC) and a photobioreactor was developed, and its effectiveness for continuous wastewater treatment and electricity production was ... A coupled system consisting of an upflow membrane-less microbial fuel cell (upflow ML-MFC) and a photobioreactor was developed, and its effectiveness for continuous wastewater treatment and electricity production was evaluated. Wastewater was fed to the upflow ML-MFC to remove chemical oxygen demand (COD), phosphorus and nitrogen with simultaneous electricity generation. The effluent from the cathode compartment of the upflow ML-MFC was then continuously fed to an external photobioreactor for removing the remaining phosphorus and nitrogen using microalgae. Alone, the upflow ML-MFC produces a maximum power density of 481 mW/m 3 , and obtains 77.9% COD, 23.5% total phosphorus (TP) and 97.6% NH4+-N removals. When combined with the photobioreactor, the system achieves 99.3% TP and 99.0% NH4+-N total removal. These results show both the effectiveness and the potential application of the coupled system to continuously treat domestic wastewater and simultaneously generate electricity and biomass. 展开更多
关键词 wastewater treatment microbial fuel cell PHOTOBIOREACTOR MICROALGAE BIOELECTRICITY
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一种基于SU8聚合物的基因测序芯片 被引量:2
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作者 韩伟静 魏清泉 +2 位作者 李运涛 周晓光 俞育德 《物理学报》 SCIE EI CAS CSCD 北大核心 2013年第14期496-503,共8页
基因测序技术极大地推动了生物学和医学研究的发展.结合了焦磷酸测序原理及阵列式微反应池芯片的高通量测序仪在从头测序和宏基因组测序方面有着不可替代的作用.本文首次提出并研制了一种基于SU8聚合物的基因测序芯片.选择了高传输效率... 基因测序技术极大地推动了生物学和医学研究的发展.结合了焦磷酸测序原理及阵列式微反应池芯片的高通量测序仪在从头测序和宏基因组测序方面有着不可替代的作用.本文首次提出并研制了一种基于SU8聚合物的基因测序芯片.选择了高传输效率、低耦合损耗的光纤面板作为基片,通过改善SU8均匀性及释放应力,在光纤面板上成功制备出百万数量级阵列式微反应池;设计并制作侧壁镀膜装置,实现了SU8阵列式微反应池侧壁选择性光学薄膜蒸镀,有效地提高了微反应池的光学隔离度,将相邻微反应池之间的光串扰率平均值从25%降低到了1%,满足了高通量焦磷酸测序对测序芯片独立并行传输弱光信号的要求.基于SU8聚合物的基因测序芯片制备工艺简单、成本低廉,具有良好的应用前景. 展开更多
关键词 DNA测序 SU8 微反应池 光纤面板
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Bimetal-organic framework-derived carbon nanocubes with 3D hierarchical pores as highly efficient oxygen reduction reaction electrocatalysts for microbial fuel cells 被引量:1
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作者 Neng Chen Zihan Meng +3 位作者 Rui Wang Shichang Cai Weibin Guo Haolin Tang 《Science China Materials》 SCIE EI CAS CSCD 2021年第12期2926-2937,共12页
Noble metal-free and highly efficient electrocatalytic materials with hierarchically porous structures continue to be studied for the oxygen reduction reaction(ORR) in microbial fuel cells(MFCs). We report bimetal-org... Noble metal-free and highly efficient electrocatalytic materials with hierarchically porous structures continue to be studied for the oxygen reduction reaction(ORR) in microbial fuel cells(MFCs). We report bimetal-organic framework(bi-MOF)-derived nanocubic Swiss cheese-like carbons with a novel three-dimensional hierarchically porous structure(3D Co-N-C) prepared by utilizing cetyltrimethylammonium bromide(CTAB) as a structure-directing agent to control the formation of a nanocubic skeleton, and silica spheres as a template to form a mesoporous structure. The elemental composition and chemical morphology of this material can be tuned through the Zn/Co ratio to optimize its ORR catalytic activity. The optimized 3D Co-N-C displays excellent ORR catalytic performance(half-wave potential as high as 0.754 V vs. reversible hydrogen electrode and diffusion-limiting current density of 5.576 mA cm^(-2)) in 0.01 mol L^(-1) phosphate-buffered saline(PBS electrolyte),showing it can compete with the commercial 20 wt% Pt/C catalysts. The catalytic capability and long-term durability of 3D Co-N-C as an air-filled cathode electrocatalyst in an MFC device are tested, showing that the 3D CoNC-MFC can reach a high power density of 1257 mW m^(-2) and provide a competitive voltage during a periodic feeding operation for 192 h;these values are much higher than those of the Pt/C-MFC. 展开更多
关键词 Co-N-doped carbon structure-oriented approach 3D hierarchically porous nanocubes oxygen reduction reaction microbial fuel cells
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Spontaneously producing syngas from MFC-MEC coupling system based on biocompatible bifunctional metal-free electrocatalyst 被引量:1
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作者 Wei Wang Rahul Anil Borse +1 位作者 Jiafang Xie Yaobing Wang 《Science China Materials》 SCIE EI CSCD 2021年第3期592-600,共9页
The preparation of high-value fuels and chemicals through the electrochemical carbon dioxide reduction reaction(CDRR)is of great significance to the virtuous cycle of carbon dioxide.However,due to the high overpotenti... The preparation of high-value fuels and chemicals through the electrochemical carbon dioxide reduction reaction(CDRR)is of great significance to the virtuous cycle of carbon dioxide.However,due to the high overpotential involved in this reaction,high power consumption and high-cost noble-metal-based catalysts are required for driving this process.Herein,the electrochemical CDRR was achieved on biocompatible metal-free nitrogen,phosphorus co-doped carbon-based materials(NP-C)in the microbial fuel cell-microbial electrolysis cell(MFC-MEC)coupling system.As the bioelectrochemistry in MFC supplied power to drive the electrocatalysis in MEC,syngas was spontaneously produced from this coupling system without external energy input.With the NP-C materials as the excellent bifunctional electrocatalyst for the CDRR and oxygen reduction reaction(ORR),the current density of the MEC reached−0.52 mA cm^−2,and the Faradaic efficiencies(FEs)of CO and H2 were 60%and 40%,respectively,at a load resistance of 10Ω.Moreover,the CO/H2 product ratio can be changed by adjusting the load resistance,which will widely meet various demand of syngas usage in further reactions.This study provides a spontaneous and tunable production of syngas in biogas digesters via a electrochemical strategy. 展开更多
关键词 spontaneous production SYNGAS MFC-MEC coupling system N/P-co-doped carbon electrocatalyst
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