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高效液相色谱测定水中微囊藻毒素 被引量:9
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作者 徐荣 张德明 卢益新 《净水技术》 CAS 2005年第5期63-65,共3页
应用高效液相色谱法测定水中微囊藻毒素的含量。水样经C18柱固相萃取后进行HPLC分析。通过优化固相萃取方法,采用SymmentryC18色谱柱,以甲醇:水(含0.05%三氟乙酸)=60:40(V/V)溶液为流动相,紫外238nm检测。对两种常见微囊藻毒素MC-RR、MC... 应用高效液相色谱法测定水中微囊藻毒素的含量。水样经C18柱固相萃取后进行HPLC分析。通过优化固相萃取方法,采用SymmentryC18色谱柱,以甲醇:水(含0.05%三氟乙酸)=60:40(V/V)溶液为流动相,紫外238nm检测。对两种常见微囊藻毒素MC-RR、MC-LR的检测限为0.02-0.05μg/L。实验表明该法可有效检测水样中的痕量微囊藻毒素。 展开更多
关键词 高效液相色谱法 微囊藻毒素水体 固相萃取 流动相 高效液相色谱法 微囊毒素 水样 测定 HPLC分析 固相萃取 C18柱 检测限 萃取方法
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Research on the Relationship between Three Isomers of Microcystins and Environmental Factors 被引量:3
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作者 张洪玲 蔡金傍 李文奇 《Agricultural Science & Technology》 CAS 2011年第9期1342-1346,共5页
[Objective] The relationship between three isomers of microcystins and environmental factors were studied in the fields.[Method] Three isomers of microcystins (MC-LR,RR and YR) from water of five sampling spots in a... [Objective] The relationship between three isomers of microcystins and environmental factors were studied in the fields.[Method] Three isomers of microcystins (MC-LR,RR and YR) from water of five sampling spots in a northern reservoir were observed for one year with High Performance Liquid Chromatography analytical method in order to study the relationship between three isomers and environmental factors.[Result] The three isomers of microcystins showed positive correlation with chlorophyll a;LR and YR isomers all had significant linear positive correlations with the water temperature,but the RR isomer showed no significant correlation with the water temperature;LR and YR isomers had relatively significantly correlativities with the contents of total nitrogen,nitrate nitrogen and organic nitrogen,while the RR isomer only showed a significant negative correlation with the content of nitrate nitrogen;LR and RR isomers both showed significant positive correlations with the contents of total phosphorus and organic phosphorus,while the phosphorus hardly affected the YR isomer and showed no evident correlation.[Conclusion] The relationship between three isomers of microcystins and environmental factors such as chlorophyll a,water temperature,nitrogen,phosphorus were studied and investigated the reasons,which might offered a reference for controlling the growth of blue algae in water and toxin synthesis. 展开更多
关键词 RESERVOIR MICROCYSTINS ISOMERS Environmental factors
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