In this paper, an approach to predicting randomly-shaped particle volume based on its two- Dimensional (2-D) digital image is explored. Conversion of gray-scale image of the particles to its binary counterpart is fi...In this paper, an approach to predicting randomly-shaped particle volume based on its two- Dimensional (2-D) digital image is explored. Conversion of gray-scale image of the particles to its binary counterpart is first performed using backlighting technique. The silhouette of particle is thus obtained, and consequently, informative features such as particle area, centroid and shape-related descriptors are collected. Several dimensionless parameters are defined, and used as regressor variables in a multiple linear regression model to predict particle volume. Regressor coefficients are found by fitting to a randomly selected sample of 501 panicles ranging in size from 4.75mm to 25ram. The model testing experiment is conducted against a different aggregate sample of the similar statistical properties, the errors of the model-predicted volume of the batch is within ±2%.展开更多
AIM: To improve the immunogenicity of receptor binding site of hepatitis B virus (HBV) on preS1 antigen using HBV core antigen as an immuno-carrier. METHODS: One to 6 tandem copies of HBV preS1 (21-47) fragment were i...AIM: To improve the immunogenicity of receptor binding site of hepatitis B virus (HBV) on preS1 antigen using HBV core antigen as an immuno-carrier. METHODS: One to 6 tandem copies of HBV preS1 (21-47) fragment were inserted into HBcAg at the sites of aa 78 and 82, and expressed in E.coli. ELISA, Western blot and animal immunization were used to analyze the antigenicity and immmunogenicity of purified particulate antigens. The ability to capture HBV by antibodies elicited by chimeric particles was detected with immuno-capture PCR. RESULTS: Recombinant antigens CI, CII, CIII carrying 1-3 copies of HBV preSl (21-47) individually could form virus-like particles (VLPs), similar to HBcAg in morphology. But recombinant antigens carrying 4-6 copies of HBV preSl (21-47) were poorly expressed in E.coli. Chimeric antigens were lacking of immunoreactivity with anti-HBc monoclonal antibodies (McAbs), but still reserved good immunoreactivity with anti-HBe McAbs. CI, CII, CIII could strongly react with anti-preS1 McAb, suggesting that preS1 (21-47) fragment was well exposed on the surface of chimeric VLPs. Three chimeric VLP antigens (CI, CII and CIII) could stimulate mice to produce high-level antibody responses, and their immunogenicity was stronger than non-particulate antigen 21-47*6, containing 6 copies of preS1 (21-47). Mouse antibodies to CI, CII and CIII were able to capture HBV virions in immuno-capture PCR assay in vitro. CONCLUSION: Chimeric particulate antigens of receptor binding site-core antigen of HBV can elicit strong antibody responses to preS1. They have a potential to be developed into prophylactic or therapeutic vaccines against HBV infection.展开更多
Abstract Picoplankton distribution was investigated in different water masses of the East China Sea in November, 2006 and February, 2007. The autumn and winter cruises crossed three major water masses: the coastal wa...Abstract Picoplankton distribution was investigated in different water masses of the East China Sea in November, 2006 and February, 2007. The autumn and winter cruises crossed three major water masses: the coastal water mass (CWM), the mixed water mass (MWM), which forms on the continental shelf, and the Kuroshio water mass (KWM). Picoplankton composition was resolved into four main groups by flow cytometry, namely Synechococcus, Prochlorococcus, picoeukaryotes, and heterotrophic bacteria. The average abundances of Synechococcus, picoeukaryotes, and heterotrophic bacteria were (0.63+ 10.88)~ 103, (1.61+1.16)x103, (3.39~1.27)x105 cells/mL in autumn and (6.45~8.60)x103, (3.23~2.63)x103, (3.76~1.37)x 105 cells/mL in winter, respectively. Prochlorococcus was not found in the CWM and seldom observed in surface samples in either season. However, Prochlorococcus was observed in the MWM and KWM (approximately 103 cells/mL) in both auttman and winter. Synechococcus distribution varied considerably among water masses, with the highest levels in KWM and lowest levels in CWM. The depth-averaged integrated abundance of Synechococcus was approximately 5-fold higher in KWM than in CWM, which may be due primarily to water temperature. In the MWM, Synechococcus was resolved as two subgroups; the presence of both subgroups was more common in autumn. Picoeukaryote abundance varied less among water masses than Synechococcus, and heterotrophic bacteria depth-averaged integrated abundance exhibited the smallest seasonal variations with respect to water mass. Correlation analysis showed that relationships between picoplankton abundances and environmental factors (temperature, nutrients, and chlorophyll a) differed among the three water masses, suggesting that the three water masses have different effects on picoplankton distribution (particularly Synechococcus).展开更多
Growing literature has demonstrated that exercise may be an effective prevention and treatment option for drug addiction. In the past few years, many studies have suggested that there were sex differences in all phase...Growing literature has demonstrated that exercise may be an effective prevention and treatment option for drug addiction. In the past few years, many studies have suggested that there were sex differences in all phases of drug addiction. However, very limited research has investigated sex differences in the effectiveness of exercise intervention in drug addiction and rehabilitation. In this mini review, we summarize the effect of sex on the results of using exercise to prevent and treat drug addiction. The studies we consider span various animal models and use multiple types of exercise to examine the effectiveness of exercise on the neurobiological mechanism of exercise rehabilitation. We believe that exercise as an adjuvant intervention strategy can be applied better in drug addiction prevention and recovery. Copyright 2014, Shanghai University of Sport. Production and hosting by Elsevier B.V. All rights reserved.展开更多
The Finite volume backward Euler difference method is established to discuss two-dimensional parabolic integro-differential equations.These results are new for finite volume element methods for parabolic integro-diffe...The Finite volume backward Euler difference method is established to discuss two-dimensional parabolic integro-differential equations.These results are new for finite volume element methods for parabolic integro-differential equations.展开更多
micro-electro-mechanical system (MEMS) device has the advantages of both electronic system and mechanical system. With the development of MEMS devices for satellite, it is possible to establish much lighter and smal...micro-electro-mechanical system (MEMS) device has the advantages of both electronic system and mechanical system. With the development of MEMS devices for satellite, it is possible to establish much lighter and smaller nanosatellites with higher performance and longer lifecyele. The power consumption of MEMS devices is usually much lower than that of traditional devices, which will greatly reduce the consumption of power. For its small size and simple architecture, MEMS devices can be easily integrated together and achieve redundancy. Launched on April 18, 2004, NS - 1 is a nanosatellite for science exploration and MEMS devices test. A mass of science data and images were acquired during its running. NS - 1 weights less than 25 kg. It consists of several MEMS devices, including one miniature inertial measurement unit(MIMU) , three micro complementary metal oxide semiconductor (CMOS)cameras, one sun sensor, three momentum wheels, and one micro magnetic sensor. By applying micro components based on MEMS technology, NS - 1 has made success in the experiments of integrative design, manufacture, and MEMS devices integration. In this paper, some MEMS devices for nanosatellite and picosatellite are introduced, which have been tested on NS -1 nanosatellite or on the ground.展开更多
This paper expounds upon the basic principle of scanning electron microscopy(SEM),the main features of image types,and different signals,and the applications and prospects in earth sciences research are reviewed.High-...This paper expounds upon the basic principle of scanning electron microscopy(SEM),the main features of image types,and different signals,and the applications and prospects in earth sciences research are reviewed.High-resolution field emission SEM allows observation and investigation of a very fine micro area in situ.Using low-vacuum mode SEM,geological insulating samples can be analyzed directly without coating,demonstrating the wide application prospect.Combined with backscatter detector(BSE),energy dispersal X-ray spectroscopy(EDS),cathodoluminescence spectrometry(CL),and electron back-scattering diffraction(EBSD),SEM can yield multiple types of information about geological samples at the same time,such as superficial microstructure,CL analysis,BSE image,component analysis,and crystal structure features.In this paper,we use examples to discuss the geological application of SEM.We stress that we should not only focus on the CL image analysis,but strengthen CL spectrum analyses of minerals.These results will effectively reveal the mineral crystal lattice defects and trace element composition and can help to reconstruct mineral growth conditions precisely.展开更多
基金Funded by the Zhejiang Provincial Educatrion Ministry (No.2004884), and the Scientific Research Start-up Foundation of Ningbo University (No.2004037).
文摘In this paper, an approach to predicting randomly-shaped particle volume based on its two- Dimensional (2-D) digital image is explored. Conversion of gray-scale image of the particles to its binary counterpart is first performed using backlighting technique. The silhouette of particle is thus obtained, and consequently, informative features such as particle area, centroid and shape-related descriptors are collected. Several dimensionless parameters are defined, and used as regressor variables in a multiple linear regression model to predict particle volume. Regressor coefficients are found by fitting to a randomly selected sample of 501 panicles ranging in size from 4.75mm to 25ram. The model testing experiment is conducted against a different aggregate sample of the similar statistical properties, the errors of the model-predicted volume of the batch is within ±2%.
基金Supported by the Excellent Scholar Incubation Plan of Ministry of Education, China
文摘AIM: To improve the immunogenicity of receptor binding site of hepatitis B virus (HBV) on preS1 antigen using HBV core antigen as an immuno-carrier. METHODS: One to 6 tandem copies of HBV preS1 (21-47) fragment were inserted into HBcAg at the sites of aa 78 and 82, and expressed in E.coli. ELISA, Western blot and animal immunization were used to analyze the antigenicity and immmunogenicity of purified particulate antigens. The ability to capture HBV by antibodies elicited by chimeric particles was detected with immuno-capture PCR. RESULTS: Recombinant antigens CI, CII, CIII carrying 1-3 copies of HBV preSl (21-47) individually could form virus-like particles (VLPs), similar to HBcAg in morphology. But recombinant antigens carrying 4-6 copies of HBV preSl (21-47) were poorly expressed in E.coli. Chimeric antigens were lacking of immunoreactivity with anti-HBc monoclonal antibodies (McAbs), but still reserved good immunoreactivity with anti-HBe McAbs. CI, CII, CIII could strongly react with anti-preS1 McAb, suggesting that preS1 (21-47) fragment was well exposed on the surface of chimeric VLPs. Three chimeric VLP antigens (CI, CII and CIII) could stimulate mice to produce high-level antibody responses, and their immunogenicity was stronger than non-particulate antigen 21-47*6, containing 6 copies of preS1 (21-47). Mouse antibodies to CI, CII and CIII were able to capture HBV virions in immuno-capture PCR assay in vitro. CONCLUSION: Chimeric particulate antigens of receptor binding site-core antigen of HBV can elicit strong antibody responses to preS1. They have a potential to be developed into prophylactic or therapeutic vaccines against HBV infection.
基金Supported by the National Basic Research Program of China(973 Program)(No.2011CB409804)the National Natural Science Foundation of China for Creative Research Groups(No.41121064)the Knowledge Innovation Program of China(No.KZCX2-YW-Q07-02)
文摘Abstract Picoplankton distribution was investigated in different water masses of the East China Sea in November, 2006 and February, 2007. The autumn and winter cruises crossed three major water masses: the coastal water mass (CWM), the mixed water mass (MWM), which forms on the continental shelf, and the Kuroshio water mass (KWM). Picoplankton composition was resolved into four main groups by flow cytometry, namely Synechococcus, Prochlorococcus, picoeukaryotes, and heterotrophic bacteria. The average abundances of Synechococcus, picoeukaryotes, and heterotrophic bacteria were (0.63+ 10.88)~ 103, (1.61+1.16)x103, (3.39~1.27)x105 cells/mL in autumn and (6.45~8.60)x103, (3.23~2.63)x103, (3.76~1.37)x 105 cells/mL in winter, respectively. Prochlorococcus was not found in the CWM and seldom observed in surface samples in either season. However, Prochlorococcus was observed in the MWM and KWM (approximately 103 cells/mL) in both auttman and winter. Synechococcus distribution varied considerably among water masses, with the highest levels in KWM and lowest levels in CWM. The depth-averaged integrated abundance of Synechococcus was approximately 5-fold higher in KWM than in CWM, which may be due primarily to water temperature. In the MWM, Synechococcus was resolved as two subgroups; the presence of both subgroups was more common in autumn. Picoeukaryote abundance varied less among water masses than Synechococcus, and heterotrophic bacteria depth-averaged integrated abundance exhibited the smallest seasonal variations with respect to water mass. Correlation analysis showed that relationships between picoplankton abundances and environmental factors (temperature, nutrients, and chlorophyll a) differed among the three water masses, suggesting that the three water masses have different effects on picoplankton distribution (particularly Synechococcus).
基金supported by grants from the Shanghai Science and Technology Commission(NO.13490503600)National Natural Science Foundation of China (NO.31171004)
文摘Growing literature has demonstrated that exercise may be an effective prevention and treatment option for drug addiction. In the past few years, many studies have suggested that there were sex differences in all phases of drug addiction. However, very limited research has investigated sex differences in the effectiveness of exercise intervention in drug addiction and rehabilitation. In this mini review, we summarize the effect of sex on the results of using exercise to prevent and treat drug addiction. The studies we consider span various animal models and use multiple types of exercise to examine the effectiveness of exercise on the neurobiological mechanism of exercise rehabilitation. We believe that exercise as an adjuvant intervention strategy can be applied better in drug addiction prevention and recovery. Copyright 2014, Shanghai University of Sport. Production and hosting by Elsevier B.V. All rights reserved.
基金Supported by the NSF of China(4080502090511009+2 种基金107020506070401560877001)
文摘The Finite volume backward Euler difference method is established to discuss two-dimensional parabolic integro-differential equations.These results are new for finite volume element methods for parabolic integro-differential equations.
文摘micro-electro-mechanical system (MEMS) device has the advantages of both electronic system and mechanical system. With the development of MEMS devices for satellite, it is possible to establish much lighter and smaller nanosatellites with higher performance and longer lifecyele. The power consumption of MEMS devices is usually much lower than that of traditional devices, which will greatly reduce the consumption of power. For its small size and simple architecture, MEMS devices can be easily integrated together and achieve redundancy. Launched on April 18, 2004, NS - 1 is a nanosatellite for science exploration and MEMS devices test. A mass of science data and images were acquired during its running. NS - 1 weights less than 25 kg. It consists of several MEMS devices, including one miniature inertial measurement unit(MIMU) , three micro complementary metal oxide semiconductor (CMOS)cameras, one sun sensor, three momentum wheels, and one micro magnetic sensor. By applying micro components based on MEMS technology, NS - 1 has made success in the experiments of integrative design, manufacture, and MEMS devices integration. In this paper, some MEMS devices for nanosatellite and picosatellite are introduced, which have been tested on NS -1 nanosatellite or on the ground.
基金supported by the National Natural Science Foundation of China(Grant No.41402031)
文摘This paper expounds upon the basic principle of scanning electron microscopy(SEM),the main features of image types,and different signals,and the applications and prospects in earth sciences research are reviewed.High-resolution field emission SEM allows observation and investigation of a very fine micro area in situ.Using low-vacuum mode SEM,geological insulating samples can be analyzed directly without coating,demonstrating the wide application prospect.Combined with backscatter detector(BSE),energy dispersal X-ray spectroscopy(EDS),cathodoluminescence spectrometry(CL),and electron back-scattering diffraction(EBSD),SEM can yield multiple types of information about geological samples at the same time,such as superficial microstructure,CL analysis,BSE image,component analysis,and crystal structure features.In this paper,we use examples to discuss the geological application of SEM.We stress that we should not only focus on the CL image analysis,but strengthen CL spectrum analyses of minerals.These results will effectively reveal the mineral crystal lattice defects and trace element composition and can help to reconstruct mineral growth conditions precisely.